Article
Plant Sciences
Zigang Liu, Xiaoyun Dong, Xiaodong Cao, Chunmei Xu, Jiaping Wei, Guoqiang Zhen, Jinxiong Wang, Hui Li, Xinlin Fang, Yin Wang, Hai Yan, Chao Mi, Caixia Zhao, Wenbo Mi
Summary: This study identified the genetic mechanism underlying the cold tolerance of winter rapeseed (Brassica napus) and discovered QTLs and candidate genes related to winter survival. These results are important for improving the cold tolerance and overwintering success of winter oilseed rape.
JOURNAL OF PLANT PHYSIOLOGY
(2022)
Article
Plant Sciences
Ali Raza, Wei Su, Ziqi Jia, Dan Luo, Yi Zhang, Ang Gao, Muhammad Azhar Hussain, Sundas Saher Mehmood, Yong Cheng, Yan Lv, Xiling Zou
Summary: Trehalose acts as a growth modulator and improves cold stress tolerance in rapeseed seedlings. The study found that exogenous trehalose mitigates the adverse effects of cold stress on rapeseed seedlings, increasing their survival rate. Trehalose regulates antioxidant and osmotic balance, participates in metabolism and signaling networks to enhance cold stress tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Lei Yan, Liu Zeng, Ali Raza, Yan Lv, Xiaoyu Ding, Yong Cheng, Xiling Zou
Summary: This study aimed to investigate the beneficial role of exogenous inositol in improving cold stress tolerance in rapeseed. The results revealed that inositol positively regulated cold tolerance by increasing calcium ion influx in rapeseed. The inositol-Ca2+ pathway was found to play a key role in cold tolerance. This study provides new insights into the mechanism of rapeseed cold tolerance and introduces a feasible method for improving rapeseed cold tolerance.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Wenbo Mi, Zigang Liu, Jiaojiao Jin, Xiaoyun Dong, Chunmei Xu, Ya Zou, Mingxia Xu, Guoqiang Zheng, Xiaodong Cao, Xinling Fang, Caixia Zhao, Chao Mi
Summary: The study compared two winter rapeseed cultivars, NS and NF, under cold stress and found that NS exhibited higher antioxidant activity and osmotic adjustment ability, and less plasma membrane injury compared to NF. Proteomic analysis revealed that NS accumulated proteins related to ROS scavenging more rapidly in lysine degradation, phenylalanine, tyrosine, and tryptophan, flavonoid biosynthesis, and ubiquinone and other terpenoid-quinone biosynthesis pathways, enhancing cold tolerance and minimizing ROS damage.
Article
Genetics & Heredity
Zaoxia Niu, Lijun Liu, Jinli Yue, Junyan Wu, Wangtian Wang, Yuanyuan Pu, Li Ma, Yan Fang, Wancang Sun
Summary: Glutathione transferases (GSTs) are the largest gene family in plants and play significant roles in regulating plant growth, development, and stress response. In this study, the researchers identified a total of 70 BraGSTs genes in the Brassica rapa species. They found that these genes were involved in the low-temperature response process of plants and were unevenly distributed across the chromosomes. The BraGSTF2 gene exhibited the highest level of expression and was found to enhance cold stress tolerance in B. rapa through its impact on antioxidant enzyme activity and osmolyte content.
Article
Biochemistry & Molecular Biology
Dan Luo, Ali Raza, Yong Cheng, Xiling Zou, Yan Lv
Summary: In this study, it was found that the transcript level of BnaMYBL17 was induced by cold stress in rapeseed. Functional analysis revealed that overexpression of BnaMYBL17 increased sensitivity to freezing stress. Transcriptionomic analysis identified 14,298 differentially expressed genes and 1321 candidate target genes related to freezing response. Verification experiments confirmed that BnaMYBL17 affects the promoter of BnaPLC1, BnaFLZ8, and BnaKOIN genes. Overall, BnaMYBL17 acts as a transcriptional repressor in regulating certain genes related to growth and development during freezing stress, providing valuable targets for molecular breeding to enhance freezing tolerance in rapeseed.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Engineering, Biomedical
Shraddha Chakraborty, Charlotte Luchena, Jonathan J. Elton, Marcel P. Schilling, Markus Reischl, Margaux Roux, Pavel A. Levkin, Anna A. Popova
Summary: In this study, a method called "Cells-to-cDNA on Chip" was developed to isolate mRNA from live cells and convert it to cDNA in individual droplets, enabling cell screening, phenotypic microscopy-based assessments, and gene expression analysis on an open DMA platform. This method provides a basis for obtaining detailed information about molecular dynamics in cultured cells.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Plant Sciences
Sameer H. Qari, Muhammad Umair Hassan, Muhammad Umer Chattha, Athar Mahmood, Maria Naqve, Muhammad Nawaz, Lorenzo Barbanti, Maryam A. Alahdal, Maha Aljabri
Summary: Cold stress is a major constraint for plant growth and development. The application of melatonin, a critical bio-stimulant, has been found to improve plant performance under cold stress by maintaining membrane integrity, water uptake, photosynthetic efficiency, and antioxidant activities.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Plant Sciences
Yi Zhang, Ali Raza, He Huang, Wei Su, Dan Luo, Liu Zeng, Xiaoyu Ding, Yong Cheng, Zhaofeng Liu, Quanan Li, Yan Lv, Xiling Zou
Summary: In this study, 35 Lhcb genes (BnLhcbs) were identified in the rapeseed genome, which play essential roles in photosynthesis and stress response. The BnLhcb3.4 gene was significantly induced in the tolerant rapeseed cultivar and down-regulated in the sensitive cultivar. Overexpression of BnLhcb3.4 gene enhanced the freezing tolerance of transgenic Arabidopsis and altered the expression of ABA-responsive genes.
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Article
Biochemistry & Molecular Biology
Xiaoyun Dong, Zigang Liu, Jiaping Wei, Guoqiang Zheng, Hui Li, Ying Wang, Haiyan Tian, Junmei Cui, Zefeng Wu, Xiaodong Cao, Chunmei Xu
Summary: BrAFP1 effectively prevents the recrystallization and growth of ice crystals. Its expression level determines the resistance of winter turnip rape plants to freezing-induced damage. This study investigates the activity of BrAFP1 promoters in different cold-tolerant rapeseed cultivars.
Article
Plant Sciences
Su Yang, Jialuo Chen, Yonghe Ding, Qian Huang, Guangna Chen, Zaid Ulhassan, Ji'an Wei, Jian Wang
Summary: This study conducted a comprehensive survey of the LOR gene family in Brassica napus and evaluated their responses to salinity and ABA stress. The results showed that BnLORs are involved in processes such as light response, hormone response, low temperature response, heat stress response, and dehydration response. Most BnLORs showed inducibility and exhibited tissue-specific expression patterns. This study enhances our understanding of the B. napus LOR gene family and provides valuable information for stress-resistant breeding.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Agronomy
Qingsong Zuo, Jingjing You, Long Wang, Jingdong Zheng, Jing Li, Chen Qian, Guobing Lin, Guang Yang, Suohu Leng
Summary: This study investigated the effects of different sowing density levels on agronomic traits and survival rate of mechanized transplanting of rapeseed seedlings. The results showed that high sowing density increased plant height but decreased leaf area, collar diameter, biomass accumulation, root to shoot ratio, and seedling fullness, which collectively reduced the survival rate after mechanized transplanting. However, a sowing density of 696 seeds per tray was recommended, as it produced high-quality seedlings suitable for mechanized transplanting with a high survival rate.
Article
Oncology
Manizheh Sajadi, Mohammad Fazilti, Habibollah Nazem, Mohammad Mahdevar, Kamran Ghaedi
Summary: This study analyzed the abnormal expression of transcription factors in colorectal cancer (CRC) and their impact on patient prognosis and related pathways. The findings showed that certain transcription factors were associated with poor prognosis, while others were associated with good prognosis. A predictive model based on the expression of these transcription factors accurately predicted patient survival rates. Additionally, co-expression network analysis revealed the involvement of these transcription factors in metastatic pathways and apoptotic pathways.
CANCER CELL INTERNATIONAL
(2022)
Article
Biochemistry & Molecular Biology
Wei Li, Xuemin Huai, Peitao Li, Ali Raza, Muhammad Salman Mubarik, Madiha Habib, Sajid Fiaz, Binbin Zhang, Jun Pan, Rao Sohail Ahmad Khan
Summary: This study identified 25 BnGPX genes in rapeseed through genome-wide analysis, revealing the evolutionary history and characteristics of GPX genes in plants, as well as their essential roles in antioxidant defense, hormone response, and abiotic stress conditions.
Article
Biochemical Research Methods
Pontus Eriksson, Nour-al-dain Marzouka, Gottfrid Sjodahl, Carina Bernardo, Fredrik Liedberg, Mattias Hoglund
Summary: In this study, we evaluated the behavior of gene-pair-based single sample predictors (SSPs) in gene expression-based multiclass prediction tasks. We found that these methods showed excellent performance, with high accuracy and informative prediction scores, in many expression-based classification tasks. However, the compatibility of gene-pair-based predictors with new datasets still needs to be verified.
Article
Plant Sciences
Yu Jeong, Claudia Corvalan, Soon Il Kwon, Sunghwa Choe
JOURNAL OF PLANT BIOLOGY
(2015)
Article
Plant Sciences
Yu Jeong Jeong, Slki Park, Su Jeoung Suh, Soon Il Kwon, Richard Cha, Yoong Eun Kim, Sunghwa Choe
JOURNAL OF PLANT BIOLOGY
(2016)
Article
Plant Sciences
Bokyung Kim, Mi Kwon, Jihyun Jeon, Burkhard Schulz, Claudia Corvalan, Yu Jeong Jeong, Sunghwa Choe
JOURNAL OF PLANT BIOLOGY
(2014)
Article
Biochemistry & Molecular Biology
Yu Jeong Jeong, Yun Shang, Beg Hab Kim, Sun Young Kim, Jae Hyo Song, June Seung Lee, Myeong Min Lee, Jianming Li, Kyoung Hee Nam
MOLECULES AND CELLS
(2010)
Article
Plant Sciences
Bokyung Kim, Yu Jeong Jeong, Claudia Corvalan, Shozo Fujioka, Seoae Cho, Taesung Park, Sunghwa Choe
Article
Biotechnology & Applied Microbiology
Yu Jeong Jeong
PLANT BIOTECHNOLOGY REPORTS
(2020)
Article
Plant Sciences
Yu Jeong Jeong, June Seung Lee, Dong-Gwan Kim
Summary: The study found that BRFP1 and BRFP2 are involved in the BR signaling pathway, affecting plant growth and development by regulating the stability of BIN2 protein.
JOURNAL OF PLANT BIOLOGY
(2022)
Article
Agronomy
HS Lee, EJ Kwon, SY Kwon, YJ Jeong, EM Lee, MH Jo, HS Kim, IS Woo, A Shinmyo, K Yoshida, SS Kwak
MOLECULAR BREEDING
(2003)
Article
Plant Sciences
SY Kwon, YJ Jeong, HS Lee, JS Kim, KY Cho, RD Allen, SS Kwak
PLANT CELL AND ENVIRONMENT
(2002)