Article
Cell Biology
Rong Huang, Liyan Sui, Cong Fu, Yanhui Zhai, Xiangpeng Dai, Sheng Zhang, Ziyi Li
Summary: Inhibition of HDAC11 significantly affects meiosis by altering acetylation levels of key molecules, leading to disrupted maturation of porcine oocytes and compromised subsequent embryo development.
Article
Plant Sciences
Jinping Cheng, Linhao Xu, Valentin Berger, Astrid Bruckmann, Chao Yang, Veit Schubert, Klaus D. Grasser, Arp Schnittger, Binglian Zheng, Hua Jiang
Summary: This study reveals that H3K9 demethylases IBM1 and JMJ27 cooperatively regulate meiotic progression and protect protein-coding genes from ectopic H3K9me2 modification in Arabidopsis thaliana. They also interact with the cohesin complex cofactor PDS5, independently of H3K9 demethylation, to regulate male meiosis and gene expression.
Review
Plant Sciences
Lu Xiong, Wenguan Zhou, Paloma Mas
Summary: The circadian clock and light signaling pathways are closely interconnected, and chromatin remodeling plays a crucial role in regulating transcription timing and light-dependent responses.
CURRENT OPINION IN PLANT BIOLOGY
(2022)
Article
Plant Sciences
Hong-Sheng Liao, Chien-Chih Yang, Ming-Hsiun Hsieh
Summary: The study reveals that HDA15-mediated histone modification regulates anthocyanin accumulation in response to N deficiency and other stresses in Arabidopsis. Different stresses activate anthocyanin biosynthesis through distinct regulatory modules.
JOURNAL OF EXPERIMENTAL BOTANY
(2022)
Article
Genetics & Heredity
Yongtao Han, Amira Haouel, Elisabeth Georgii, Santiago Priego-Cubero, Christoph J. Wurm, Daniel Hemmler, Philippe Schmitt-Kopplin, Claude Becker, Joerg Durner, Christian Lindermayr
Summary: Histone deacetylation by HD2A and HD2B regulates gene expression and ABA biosynthesis, leading to enhanced drought resistance in Arabidopsis. The hd2ahd2b mutant displays hypersensitivity to exogenous ABA and shows upregulation of ABA-responsive genes and H4K5ac levels. HD2A/HD2B directly bind to specific ABA-responsive genes and repress ABA biosynthesis by deacetylating H4K5ac at NCED9. These findings highlight the important role of HD2A and HD2B in ABA signaling and their negative regulation in drought resistance.
Article
Cell Biology
Hao Qin, Yi Qu, Yi-Feng Yuan, Yang-Yang Li, Jie Qiao
Summary: This study revealed that RBM14 is down-regulated in oocytes from old mice and shows overlapped localization patterns with alpha-tubulin in different stages of meiosis. Knockdown of RBM14 may result in symmetric division of oocytes, spindle defects, and chromosome abnormalities. Additionally, RBM14 interacts with endogenous alpha-tubulin and modulates oocyte meiotic maturation by affecting alpha-tubulin acetylation.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Cell Biology
Joanna Moronczyk, Agnieszka Braszewska, Barbara Wojcikowska, Karolina Chwialkowska, Katarzyna Nowak, Anna M. Wojcik, Miroslaw Kwasniewski, Malgorzata D. Gaj
Summary: Somatic embryogenesis (SE) is a complex process involving the reprogramming of plant somatic cells, and histone acetylation plays a crucial role in regulating the expression of key transcription factor genes during SE. This study investigated the global and gene-specific changes in histone acetylation in Arabidopsis explants undergoing SE and identified H3 and H4 acetylation as potential regulators of critical TF genes. The findings also revealed that HDA19 negatively affects SE by regulating LEC1, LEC2, and BBM, and provided evidence of HDA19's involvement in histone acetylation-mediated regulation of LEC2 during SE.
Article
Cell Biology
Fabien Moretto, N. Ezgi Wood, Minghao Chia, Cai Li, Nicholas M. Luscombe, Folkert J. van Werven
Summary: In this study, it is found that the transcription levels of the noncoding region IRT2 in S. cerevisiae can regulate opposing chromatin and transcription states, thus affecting the expression of the IME1 promoter and meiotic entry. The findings illustrate how intergenic transcription is crucial in controlling local chromatin state, gene expression, and cell fate outcomes.
Article
Biochemistry & Molecular Biology
Lele Song, Xinyi Yao, Hangpeng Li, Bo Peng, Alan P. Boka, Yiman Liu, Guochao Chen, Zhenyang Liu, Kaeli M. Mathias, Lingbo Xia, Qinglan Li, Mustafa Mir, Yuanyuan Li, Haitao Li, Liling Wan
Summary: Growing evidence suggests the presence of transcriptional condensates on chromatin, but their formation mechanisms and functional significance are still unclear. This study reveals that mutations in the histone acetylation reader ENL can lead to aberrant condensate formation, which drives oncogenic gene activation in human cancer.
Editorial Material
Endocrinology & Metabolism
Natalie Niemi
Summary: Previous studies suggested that acetylation of mitochondrial proteins occurs non-enzymatically, but a new research challenges this notion by demonstrating the involvement of a histone acetyltransferase in enhancing mitochondrial metabolism through acetylating a complex IV assembly factor.
Article
Plant Sciences
Yongtao Han, Elisabeth Georgii, Santiago Priego-Cubero, Christoph J. Wurm, Patrick Huether, Gregor Huber, Robert Koller, Claude Becker, Joerg Durner, Christian Lindermayr
Summary: Seed dormancy is a crucial developmental transition that affects plant adaptation and survival. In this study, it was found that the loss of function of two plant-specific histone deacetylases, HD2A and HD2B, enhanced seed dormancy in Arabidopsis. Silencing of HD2A and HD2B resulted in hyperacetylation of the DOG1 locus and increased DOG1 expression during seed maturation and imbibition. The study also revealed the interaction between HD2A, HD2B, HSI2, and HSL1. Overall, these findings suggest that HSI2 and HSL1 may recruit HD2A and HD2B to negatively regulate DOG1 expression and reduce seed dormancy, thus affecting seed development and germination.
FRONTIERS IN PLANT SCIENCE
(2023)
Review
Biochemistry & Molecular Biology
Kwon Young Chou, Jun-Yeong Lee, Kee-Beom Kim, Eunjeong Kim, Hyun-Shik Lee, Hong-Yeoul Ryu
Summary: The budding yeast Saccharomyces cerevisiae is a widely used model system for studying histone modifications and chromatin states. Recent advances in biotechnology have allowed for the detection and quantification of protein post-translational modifications, leading to the identification of new modification sites in histones. Understanding the cellular functions of these new histone markers is crucial for unraveling epigenetic mechanisms and their impact on biological processes.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2023)
Article
Plant Sciences
Miao Zhang, Feng-Quan Tan, Yan-Jie Fan, Ting-Ting Wang, Xin Song, Kai-Dong Xie, Xiao-Meng Wu, Fan Zhang, Xiu-Xin Deng, Jude W. Grosser, Wen-Wu Guo
Summary: Polyploidization leads to novel phenotypes, and the reprogramming of lysine acetylation plays an important role in trait evolution during this process, by regulating enzyme activity and protein abundance.
Article
Multidisciplinary Sciences
Longfei Zhu, Nadia Fernandez-Jimenez, Maja Szymanska-Lejman, Alexandre Pele, Charles J. Underwood, Heidi Serra, Christophe Lambing, Julia Dluzewska, Tomasz Bieluszewski, Monica Pradillo, Ian R. Henderson, Piotr A. Ziolkowski
Summary: This study reveals the importance of the SMC5/6 complex in ensuring the proper progress of meiotic recombination in plants, showing the impact of SNI1 mutations and interactions with other genes on crossover distribution.
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2021)
Article
Plant Sciences
Chae Jin Lim, Ki Suk Park, Akhtar Ali, Junghoon Park, Seung Min Ryou, Mingzhe Shen, Haris Ali Khan, Zein Eddin Bader, Shah Zareen, Min Jae Bae, Jong Hyoo Choi, Zheng-Yi Xu, Jose M. Pardo, Dae-Jin Yun
Summary: The Arabidopsis HOS15/PWR/HDA9 repressor complex negatively regulates flowering time by deacetylating the AGL19 promoter, leading to early flowering.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Oncology
Giuseppina Bruno, Valeria Li Bergolis, Annamaria Piscazzi, Fabiana Crispo, Valentina Condelli, Pietro Zoppoli, Francesca Maddalena, Michele Pietrafesa, Guido Giordano, Danilo Swann Matassa, Franca Esposito, Matteo Landriscina
Summary: The study reveals the role of the TRAP1 gene in the adaptive response of human colorectal cancer cells to hypoxia. TRAP1 is involved in regulating HIF-1α stabilization, glycolytic metabolism, and gene expression reprogramming. It also affects glucose uptake, lactate production, and ribosome biogenesis through the mTOR pathway.
INTERNATIONAL JOURNAL OF ONCOLOGY
(2022)
Article
Oncology
Donatella Malanga, Carmelo Laudanna, Teresa Mirante, Fabiana Colelli, Simona Migliozzi, Pietro Zoppoli, Gianluca Santamaria, Luca Roberto, Carmela De Marco, Marzia Scarfo, Donatella Montanaro, Orlando Paciello, Serenella Papparella, Chiara Mignogna, Alfonso Baldi, Giuseppe Viglietto
Summary: The gain-of-function mutation AKT1E17K is demonstrated to be a bona fide oncogene for mammary epithelium, capable of efficiently initiating breast cancer in mice. The AKT1E17K strain may serve as an appropriate model for human basal-like breast cancer and may aid in the identification of novel therapies specific to this type of tumor.
Article
Plant Sciences
Molla F. Mengist, Hamed Bostan, Domenico De Paola, Scott J. Teresi, Adrian E. Platts, Gaetana Cremona, Xinpeng Qi, Ted Mackey, Nahla Bassil, Hamid Ashrafi, Lara Giongo, Rubina Jibran, David Chagne, Luca Bianco, Mary A. Lila, Lisa J. Rowland, Marina Iovene, Patrick P. Edger, Massimo Iorizzo
Summary: Understanding the recombination behavior in polyploidy species is crucial for genetic discoveries. This study focused on blueberry, a tetraploid species, and investigated its genetic behavior using cytogenetics and high-density genetic maps. The study revealed the presence of an autotetraploid behavior and a translocation affecting chromosome pairing and recombination. No other structural genomic divergences were detected, providing valuable resources for future genetic and comparative genomic studies.
Review
Agronomy
Serena Varotto, Tamar Krugman, Riccardo Aiese Cigliano, Khalil Kashkush, Ankica Kondic-Spika, Fillipos A. Aravanopoulos, Monica Pradillo, Federica Consiglio, Riccardo Aversano, Ales Pecinka, Dragana Miladinovic
Summary: Crop wild relatives (CWRs) possess epigenetic diversity that can contribute novel epialleles for crop improvement. Conservation and monitoring of epigenotypes can help preserve agrobiodiversity. Integrating epigenetics into breeding programs using advanced tools is a promising approach.
THEORETICAL AND APPLIED GENETICS
(2022)
Article
Pharmacology & Pharmacy
Ester Pagano, Barbara Romano, Donatella Cicia, Fabio A. Iannotti, Tommaso Venneri, Giuseppe Lucariello, Maria Francesca Nani, Fabio Cattaneo, Paola De Cicco, Maria D'Armiento, Marcello De Luca, Ruggiero Lionetti, Stefania Lama, Paola Stiuso, Pietro Zoppoli, Geppino Falco, Silvia Marchiano, Stefano Fiorucci, Raffaele Capasso, Vincenzo Di Marzo, Francesca Borrelli, Angelo A. Izzo
Summary: TRPM8 is overexpressed in colorectal cancer and its high expression is associated with lower survival rate of patients and hyperactivation of related signaling pathways. TRPM8 may serve as a prognostic marker and potential drug target for colorectal cancer.
BRITISH JOURNAL OF PHARMACOLOGY
(2023)
Article
Agriculture, Dairy & Animal Science
Giorgia Gioacchini, Sara Filippi, Rossella Debernardis, Luca Marisaldi, Riccardo Aiese Cigliano, Oliana Carnevali
Summary: This study aimed to evaluate the cross-talk among metabolism, stress response, immune system and reproduction in immature and mature female swordfish. Transcriptomic and histological analysis revealed that mature females have compromised detoxification capacity and immune system, indicating higher susceptibility to environmental stress and pollutants during the reproductive season.
Article
Agriculture, Dairy & Animal Science
Monica Ambrosio, Francesca Paola Nocera, Francesca Garofalo, Pasquale De Luca, Alex Grinberg, Luisa De Martino
Summary: The occurrence of Staphylococcus microti (S. microti) in milk and milking parlor equipment samples of an Italian buffalo farm was assessed in this study. The results showed that S. microti was commonly found in buffalo milk and on milking parlor surfaces, with 20.4% of the isolates exhibiting multidrug resistance. This study highlights the importance of milking parlor environmental quality in the spread of S. microti and the microbial communities in milk.
Article
Biochemistry & Molecular Biology
Ciro Gianmaria Amoroso, Daniela D'Esposito, Riccardo Aiese Cigliano, Maria Raffaella Ercolano
Summary: This study analyzed and compared the transcriptomic responses of resistant and susceptible tomato genotypes to multiple biotic and abiotic stresses. Many genes involved in defense mechanisms were identified, including those encoding for TFs, phytohormones, and participating in signaling and cell wall metabolic processes. A total of 1474 DEGs were commonly found between biotic and abiotic stress, with 67 DEGs involved in response to at least four different stresses. The detected genes responsive to multiple stress could be further studied and utilized to enhance plant tolerance through biotechnological approaches.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Andrology
Celia Corral-Vazquez, Joan Blanco, Riccardo Aiese Cigliano, Sarrate Zaida, Francesca Vidal, Ester Anton
Summary: The study used Next Generation Sequencing (NGS) to characterize the viral and bacterial RNA cargo of human sperm cells from healthy fertile donors. RNA-seq raw data from 12 sperm samples were aligned to microbiome databases and analyzed using GAIA software. The results showed common microbiome patterns among the samples and identified viruses and bacteria that make up the human sperm microbiome.
SYSTEMS BIOLOGY IN REPRODUCTIVE MEDICINE
(2023)
Article
Agriculture, Dairy & Animal Science
Anna Maria Sutera, Francesca Arfuso, Giuseppe Tardiolo, Valentina Riggio, Francesco Fazio, Riccardo Aiese Cigliano, Andreu Paytuvi, Giuseppe Piccione, Alessandro Zumbo
Summary: This study investigated the potential impact of a co-feed liquid whey-integrated diet on the fecal microbiota of crossbred pigs. The results showed that the supplementation with liquid whey affected the diversity and abundance of the bacterial community in the pig's feces. Furthermore, the correlation analysis suggested that the identified differentially abundant genera were associated with the physiological parameters of the pigs.
Article
Agriculture, Dairy & Animal Science
Giuseppe Tardiolo, Orazio Romeo, Alessandro Zumbo, Marco Di Marsico, Anna Maria Sutera, Riccardo Aiese Cigliano, Andreu Paytuvi, Enrico D'Alessandro
Summary: Liquid feeding is an alternative method in swine production that utilizes low-cost liquid by-products to reduce environmental impact. The study investigated the fecal microbiota of the Nero Siciliano pig breed fed a liquid whey-supplemented diet. The results showed changes in the abundance of certain bacterial genera, indicating a potential effect of the diet on the microbial community.
Review
Engineering, Marine
Viviana Di Tuccio, Pasquale De Luca, Giovanna Romano
Summary: The sea urchin embryo is a widely used model system to study biological processes and investigate molecular and cellular responses to external stress factors. Programmed cell death (PCD) is a regulated molecular mechanism that plays a central role in tissue rearrangement and development, especially during metamorphosis, and is activated in response to abiotic stress. This review summarizes the current knowledge about the involvement of PCD processes in sea urchin embryos exposed to pollutants and provides information about the occurrence of physiological apoptosis during development.
JOURNAL OF MARINE SCIENCE AND ENGINEERING
(2023)
Article
Genetics & Heredity
Bruna De Felice, Concetta Montanino, Claudia Pinelli, Massimo Nacca, Pasquale De Luca
Summary: Palmoplantar keratoderma is a group of skin diseases characterized by thickening of the palms and soles. Various genetic mutations, such as KRT9, KRT1, AQP5 and SERPINB 7, have been identified as triggers for palmoplantar keratoderma. In this study, a family with palmoplantar keratoderma caused by autosomal dominant KRT1 mutations was analyzed for KRT1 genetic sequence, telomerase activity, and miR-21 expression. The patients showed thickening of the skin on their hands and feet, KRT1 mutations, and high expression levels of hTERT, hTR, and miR-21, indicating aberrant proliferation and inflammation.
Article
Microbiology
Erika Arcadi, Emanuela Buschi, Eugenio Rastelli, Michael Tangherlini, Pasquale De Luca, Valentina Esposito, Rosario Calogero, Franco Andaloro, Teresa Romeo, Roberto Danovaro
Summary: Current knowledge of the microbial diversity of shallow-water hydrothermal vents is limited. This study used 16S rRNA gene metabarcoding to investigate the bacteria and archaea in seawater and sediments of three shallow-water hydrothermal systems. The results showed significant differences in microbial diversity between seawater and sediments. Additionally, the benthic microbial assemblages differed markedly among the three areas, reflecting the different hydrothermal activities. Overall, this study suggests that hydrothermal vent dynamics contribute to an increase in microbial diversity in this unique system.
Article
Medicine, Research & Experimental
Ilaria Laurenzana, Luciana De Luca, Pietro Zoppoli, Giovanni Calice, Alessandro Sgambato, Angelo Michele Carella, Antonella Caivano, Stefania Trino
Summary: The methylation profile of HSPCs following AHSCT was analyzed and found to undergo changes that may be useful in transplant diagnostics. The methylation signature was also found to be related to patient outcome. This study suggests that the methylation profile of HSPCs can provide prognostic information for engraftment success and graft failure prediction in AHSCT.
CLINICAL AND EXPERIMENTAL MEDICINE
(2023)
