Article
Multidisciplinary Sciences
Yasunori Saitoh, Namiki Mitani-Ueno, Keisuke Saito, Kengo Matsuki, Sheng Huang, Lingli Yang, Naoki Yamaji, Hiroshi Ishikita, Jian-Ren Shen, Jian Feng Ma, Michihiro Suga
Summary: The authors have shown the crystal structure of rice Lsi1 and identified a unique hydrophilic selectivity filter composed of five residues. This structure provides a solid basis for the highly selective activity of Lsi1 in silicon uptake, contributing to secure and sustainable rice production.
NATURE COMMUNICATIONS
(2021)
Review
Biochemistry & Molecular Biology
Fabio Herrera, Olga Bondarenko, Sergii Boryshpolets
Summary: Fish spermatozoa adapt to environmental changes to maintain cell structure and functional integrity, which is essential for their motility and successful fertilization. Specific water channels may play a role in cell volume regulation and motility. Changes in external osmolality can impact sperm function and quality.
FISH PHYSIOLOGY AND BIOCHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Bijay Kumar Behera, Janmejay Parhi, Budheswar Dehury, Ajaya Kumar Rout, Ananya Khatei, Asem Lembika Devi, Sagar Chandra Mandal
Summary: This study aimed to elucidate the molecular mechanism of water transportation in AQP1 from walking catfish. Computational modelling and molecular dynamics simulation were used to explore the structural dynamics of CbAQP1. Unique structural dynamics and lipid binding sites were identified, providing insights into the transportation of water and small molecules in high osmotic environments.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Plant Sciences
Mengmeng Liu, Janusz J. Zwiazek
Summary: This study found that the oxidative burst had a decisive impact on the hydraulic recovery of plants upon re-aeration after root hypoxia. The gradual recovery of root hydraulic conductivity (Lpr) was accompanied by up-regulation of aquaporins and activation of antioxidant enzymes. The ability of plants to recover from waterlogging was partly determined by their ability to handle oxidative stress caused by both hypoxia event and the burst of reactive oxygen species (ROS).
ENVIRONMENTAL AND EXPERIMENTAL BOTANY
(2022)
Review
Plant Sciences
Vadim Volkov, Heiner Schwenke
Summary: This review summarizes the current knowledge on root exudation phenomenon, highlighting the changes in understanding over time due to experimental opportunities and new ideas from various knowledge areas. Recent advances in ion and water transport research at the molecular level suggest potential future directions for understanding root exudation.
Article
Developmental Biology
Bryan A. McLendon, Avery C. Kramer, Heewon Seo, Robert C. Burghardt, Fuller W. Bazer, Guoyao Wu, Gregory A. Johnson
Summary: This study determined the expression of four aquaporin proteins in the porcine uterus and placenta and found that pigs can potentially use these proteins to transport water between the endometrial bloodstream and allantoic fluid. This finding may explain the mechanism for changing volumes of allantoic fluid and hydration of allantoic connective tissues during pregnancy.
Article
Biochemistry & Molecular Biology
Meng Yang, Jingbo Liu, Yajuan Li, Qi Yang, Xuanting Liu, Chunmei Liu, Sitong Ma, Boqun Liu, Ting Zhang, Hang Xiao, Zhiyang Du
Summary: This study successfully developed an oral co-delivery system for egg white derived peptides (EWDP) and curcumin using a self-assembled amphiphilic system, showing ideal colloidal properties and encapsulation capacity at pH 2.0-7.0. Results indicated that hydrogen bonding and hydrophobic interaction were the main driving force for the formation of amphiphilic nanoparticles, which exhibited superior bioavailability compared to simple mixtures and conventional curcumin inclusion complexes.
Article
Plant Sciences
Suan Chin Wong, Martin J. Canny, Meisha Holloway-Phillips, Hilary Stuart-Williams, Lucas A. Cernusak, Diego A. Marquez, Graham D. Farquhar
Summary: Investigating the water saturation level within leaves reveals a mechanism for maintaining photosynthesis and vascular flow in dry conditions. Accurate values of humidity inside the substomatal cavity are needed to understand plant physiology and climate. This study found that as the difference in saturation water vapor concentration between the substomatal cavity and the air increases, the saturation edge retreats into the intercellular air spaces, accompanied by the closure of mesophyll aquaporins.
Article
Plant Sciences
Alicia Pou, Charles Hachez, Valentin Couvreur, Laurie C. Maistriaux, Ahmed Ismail, Francois Chaumont
Summary: The regulation of root nitrogen acquisition through mass flow involves the coordinated regulation of root hydraulic properties and stomatal conductance. This study examined the short-term effect of high nitrogen availability on hydraulic parameters and aquaporin regulation in maize genotypes. Results showed that some genotypes had a rapid increase in root hydraulic conductivity under high nitrogen treatment, while others did not. There was a significant correlation between root hydraulic conductivity and cortex cell hydraulic conductivity. However, gas exchange parameters showed genotype-specific variations that were not always correlated with root hydraulic parameters. The protein abundance of plasma membrane PIP aquaporins was correlated with the rapid variation in root hydraulic conductivity in one genotype, suggesting posttranslational mechanisms in the regulation of root hydraulic parameters in response to high nitrogen.
PHYSIOLOGIA PLANTARUM
(2022)
Review
Neurosciences
Paul J. Donaldson, Rosica S. Petrova, Nikhil Nair, Yadi Chen, Kevin L. Schey
Summary: The ocular lens is an important determinant of overall vision quality. It operates an internal microcirculation system to maintain its transparency and refractive properties. Water flow generates a pressure gradient, regulated by a dual feedback system, that determines the lens geometry and refractive index gradient. Aquaporin water channels and TRPV1/4 channels play roles in mediating lens water fluxes and membrane trafficking, affecting lens water content and pressure. Extrinsic modulation of lens water flow may alter lens refractive properties.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Biochemistry & Molecular Biology
Hooman Hadidi, Reza Kamali, Alireza Binesh
Summary: In this article, molecular dynamics were used to study the gating mechanism of Aquaporin-2 channels, identifying the important role of the selectivity filter conformation in regulating water permeation. The narrow conformation of the selectivity filter was found to hinder water molecule transport, suggesting its crucial role in modulating water permeation.
PROTEINS-STRUCTURE FUNCTION AND BIOINFORMATICS
(2021)
Article
Physics, Multidisciplinary
C. J. Moll, J. Versluis, H. J. Bakker
Summary: By studying the bending mode, we can obtain structural and quantitative information about both the surface and the bulk of water.
PHYSICAL REVIEW LETTERS
(2021)
Article
Economics
Mike G. Tsionas, Dionisis Philippas, Nikolaos Philippas
Summary: This paper proposes a multivariate asymmetric stochastic volatility approach to detect and measure herding behavior in asset returns. Applying this approach to the constituents of the S&P 500 energy sector, the findings reveal valuable information about herding detection and its relationship with asset returns' co-movements and volatility. The paper also examines the influence of macroeconomic indicators' uncertainty on the common factors of herding detection.
Article
Chemistry, Analytical
Qun Ma, Rongsheng Wang, Pengcheng Gao, Yu Dai, Fan Xia
Summary: The nanofluidic ionic signal is influenced by the interactions between ion species and the surface charge, surface wettability, and pore diameter of nanofluidic membranes. However, the impact of surface wettability on the ionic detection signal remains poorly understood. In this study, the effect of surface wettability on the ionic signal for the detection of hydrophobic drug molecules was investigated, and it was found that this signal could be tuned by light or the presence of certain ions. The regulation mechanism primarily relies on specific hydrophobic interactions, leading to a more sensitive ionic signal compared to traditional nanofluidic membranes.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Physical
Zeinab Rahimi, Amir Lohrasebi
Summary: This study investigates the effects of constant and GHz electric fields on the dynamics of glycerol and water molecules inside the AQP7 channel. The presence of the 0.2 mV/nm and 10 GHz fields reduces the free energy profile of glycerol permeation. Additionally, the electric field of 0.2 mV/nm assists water transport through the channel without significant effects on channel stability. These findings shed light on how these fields can alter the normal functioning of protein channels, potentially contributing to disease development or treatment.
EUROPEAN PHYSICAL JOURNAL E
(2023)
Article
Neurosciences
Soren N. Andreassen, Trine L. Toft-Bertelsen, Jonathan H. Wardman, Rene Villadsen, Nanna MacAulay
Summary: This study provides important molecular tools for studying pathologies related to disturbed brain fluid homeostasis by analyzing the transcriptome of the choroid plexus in rats. The results show similarities in transport protein transcript abundance between species and provide a list of potential transport mechanisms and regulatory candidates involved in cerebrospinal fluid secretion.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Article
Neurosciences
Trine Lisberg Toft-Bertelsen, Dagne Barbuskaite, Eva Kjer Heerfordt, Sara Diana Lolansen, Soren Norge Andreassen, Nina Rostgaard, Markus Harboe Olsen, Nicolas H. Norager, Tenna Capion, Martin Fredensborg Rath, Marianne Juhler, Nanna MacAulay
Summary: The study reveals that a serum lipid can promote excessive CSF secretion and subsequent brain water accumulation by acting on TRPV4, which may be a potential pharmacological target for pathologies involving brain water accumulation.
FLUIDS AND BARRIERS OF THE CNS
(2022)
Article
Neurosciences
Nina Rostgaard, Markus Harboe Olsen, Maud Ottenheijm, Lylia Drici, Anja Hviid Simonsen, Peter Plomgaard, Hanne Gredal, Helle Harding Poulsen, Henrik Zetterberg, Kaj Blennow, Steen Gregers Hasselbalch, Nanna MacAulay, Marianne Juhler
Summary: This study aimed to compare the molecular composition of cerebrospinal fluid (CSF) obtained from the ventricular and the lumbar compartments and evaluate the feasibility of using lumbar CSF as a proxy for ventricular CSF. Results showed differences in protein composition and distribution of clinically employed biomarkers between the two compartments. However, for certain proteins and biomarkers, lumbar CSF can be reliably used as a proxy for ventricular CSF if a lumbar/cranial index has been established. It is important to verify the compartmental preference before extrapolating from lumbar CSF to ventricular fluid.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Neurosciences
Pooya Razzaghi Khamesi, Vasileios Charitatos, Eva K. K. Heerfordt, Nanna MacAulay, Vartan Kurtcuoglu
Summary: A computational model was developed to examine the role of the standing osmotic gradient mechanism in cerebrospinal fluid (CSF) production by the ventricular choroid plexus (ChP). The model suggests that the standing osmotic gradients have minimal effect on CSF production and are unlikely to be the main drivers.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Neurosciences
Jonathan H. Wardman, Mette N. Jensen, Soren N. Andreassen, Bjarne Styrishave, Jens E. Wilhjelm, Alexandra J. Sinclair, Nanna MacAulay
Summary: Idiopathic intracranial hypertension (IIH) is characterized by increased intracranial pressure, impaired vision, and headache. This study investigated the role of obesity and hormonal perturbations in IIH pathophysiology using rat models. The results suggest that obesity-induced androgen dysregulation may contribute to the disease mechanism of IIH.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Neurosciences
Annette Buur Steffensen, Beatriche Louise Edelbo, Dagne Barbuskaite, Soren Norge Andreassen, Markus Harboe Olsen, Kirsten Moller, Nanna MacAulay
Summary: Maintaining intracranial pressure (ICP) within the physiological range is crucial for proper brain function. Fluctuations in ICP during the light-dark phase cycle may complicate diagnosis and treatment choice for patients with pressure-related disorders. These fluctuations are likely caused by circadian or sleep-wake cycle-mediated modulation of cerebrospinal fluid (CSF) flow dynamics, which may also play a role in diurnal regulation of brain waste clearance.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Biochemistry & Molecular Biology
Nina Rostgaard, Markus Harboe Olsen, Tenna Capion, Nanna MacAulay, Marianne Juhler
Summary: This study aimed to identify inflammatory biomarkers in cerebrospinal fluid (CSF) that can predict the development of post-hemorrhagic hydrocephalus (PHH) and shunt-dependency, as well as functional outcome in patients with subarachnoid hemorrhage (SAH). Proximity extension assay (PEA) was used to analyze CSF samples from 31 SAH patients, and 7 markers were identified as predictors of shunt dependency and 4 markers as predictors of functional outcome. These inflammatory biomarkers hold potential as predictive tools in the clinic.
Article
Biochemistry & Molecular Biology
Sara Diana Lolansen, Nina Rostgaard, Tenna Capion, Nicolas H. Norager, Markus Harboe Olsen, Marianne Juhler, Tiit Illimar Mathiesen, Nanna MacAulay
Summary: The molecular mechanisms underlying the development of posthemorrhagic hydrocephalus (PHH) remain unclear, but elevated cerebrospinal fluid (CSF) osmolality is not the cause of PHH. The pathological CSF accumulation observed in these patients is instead attributed to other pathological alterations associated with the abnormal presence of blood within the CSF compartments following subarachnoid hemorrhage (SAH).
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Neurosciences
Sara Diana Lolansen, Dagne Barbuskaite, Fenghui Ye, Jianming Xiang, Richard F. Keep, Nanna MacAulay
Summary: This study elucidated the molecular mechanisms underlying the development of hydrocephalus in spontaneously hypertensive rats (SHRs), and found that it is not associated with elevated intracranial pressure (ICP) and does not require increased cerebrospinal fluid (CSF) secretion or inefficient CSF drainage. This type of hydrocephalus is not life-threatening and occurs due to unknown disturbances in CSF dynamics.
FLUIDS AND BARRIERS OF THE CNS
(2023)
Article
Biochemistry & Molecular Biology
Trine L. Toft-Bertelsen, Soren Norge Andreassen, Nina Rostgaard, Markus Harboe Olsen, Nicolas H. Norager, Tenna Capion, Marianne Juhler, Nanna Macaulay
Summary: The lipidomic signature of CSF in patients with subarachnoid hemorrhage suggests dysregulation of lipids and potential biomarkers for future treatment options.
Review
Clinical Neurology
Shai David Ben-Shoshan, Sara Diana Lolansen, Tiit Illimar Mathiesen, Nanna Macaulay
Summary: This systematic review aims to explore the molecular mechanisms underlying posthemorrhagic hydrocephalus (PHH) by evaluating existing literature on increased CSF secretion and impaired CSF absorption. The results suggest that the pathological accumulation of cerebrospinal fluid in PHH may arise from a combination of increased secretion and impaired absorption, which may manifest at different time scales following a hemorrhagic event.
ACTA NEUROCHIRURGICA
(2023)
Article
Multidisciplinary Sciences
Beatriche Louise Edelbo, Soren Norge Andreassen, Annette Buur Steffensen, Nanna Macaulay, Christian Metallo
Summary: The cerebrospinal fluid (CSF) provides mechanical protection for the brain and serves as a brain dispersion route for nutrients, hormones, and metabolic waste. CSF secretion rate is elevated in the dark phase and shows similarities in day-active humans and nocturnal rats, suggesting circadian regulation. Transcriptomic and metabolomic analysis reveals significant fluctuation in CSF metabolites and differential expression of choroid plexus genes between light and dark phases, indicating light-dark phase-mediated changes in rat choroid plexus and its surrounding CSF. These findings provide insights into the molecular pathways governing CSF dynamics and potential applications in pathology.
Article
Cell Biology
Nanna Macaulay, Trine L. Toft-Bertelsen
Summary: The choroid plexus is a crucial tissue in the brain that secretes cerebrospinal fluid and maintains electrolyte balance. It is regulated by various membrane transporters and plays a vital role in controlling potassium levels in the brain. This dual function of the choroid plexus is important for overall brain function.
Article
Clinical Neurology
Nina Rostgaard, Markus Harboe Olsen, Sara Diana Lolansen, Nicolas Hernandez Norager, Peter Plomgaard, Nanna Macaulay, Marianne Juhler
Summary: By using a proteomic approach, this study aimed to identify potential protein biomarkers in cerebrospinal fluid (CSF) that can differentiate between obstructive and communicating hydrocephalus, as well as predict surgical outcomes. The analysis identified several proteins with differential abundance in CSF from hydrocephalus patients compared to control subjects, and identified ten proteins that can predict response to surgery in obstructive hydrocephalus.
ACTA NEUROCHIRURGICA
(2023)
Meeting Abstract
Neurosciences
Dennis B. Jensen, Dagne Barbuskaite, Nanna MacAulay
FLUIDS AND BARRIERS OF THE CNS
(2022)