Article
Cell & Tissue Engineering
XiaoLing Cui, XiaoTan Wang, Jie Wen, Xiao Li, Nan Li, XuXiao Hao, BaoXiang Zhao, Xunwei Wu, JunYing Miao
Summary: This study demonstrates that the small chemical molecule CPP can effectively induce the differentiation of human dermal fibroblasts (HDFs) into vascular endothelial cells (VECs). The results show that CPP treatment leads to morphological changes in HDFs and upregulation of VEC markers. Additionally, in vitro and in vivo experiments confirm that CPP-induced HDFs possess the functions of VECs. The induction of VEC differentiation by CPP is associated with the increased expression of pro-angiogenic factors.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Multidisciplinary Sciences
Yulia Solomonov, Nurit Hadad, Oleg Pikovsky, Rachel Levy
Summary: Collagen, a major protein in skin, is damaged by UV exposure, which accelerates photo-aging. Lumenato protects against collagen-3 damage caused by activated neutrophils and induces collagen-3 synthesis.
Article
Multidisciplinary Sciences
M. M. Yinhua Zhao, M. M. Yanyu Shi, Huang Lin
Summary: Recent studies suggest hypoxia promotes adipose-derived stem cells (ADSCs) to attenuate hypoxia/reoxygenation (H/R)-induced damage to human dermal microvascular endothelial cells (HDMECs). Extracellular vesicles (EVs) from ADSCs play a crucial role in regenerative medicine. This study investigated the effect of EVs from hypoxia-pretreated ADSCs on HDMECs and found that they effectively attenuate H/R-induced damage through autophagy activation and inhibiting apoptosis and oxidative stress.
Article
Biochemistry & Molecular Biology
Yoshie Umehara, Miho Takahashi, Hainan Yue, Juan Valentin Trujillo-Paez, Ge Peng, Hai Le Thanh Nguyen, Ko Okumura, Hideoki Ogawa, Francois Niyonsaba
Summary: The skin produces antimicrobial peptides that not only fight against pathogens but also have immunomodulatory functions. This study found that human beta-defensins promote the secretion of angiogenin, a potent angiogenic factor, by fibroblasts, suggesting a novel role of these peptides in angiogenesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Geriatrics & Gerontology
Yuying Guan, Jia Liu, Yakun Gu, Xunming Ji
Summary: Cerebrovascular microcirculation is crucial for brain function, and angiogenesis plays a significant role in remodeling the microcirculation. Hypoxia is an important regulator of angiogenesis, but it also negatively affects cerebral vascular tissue. Establishing a model that promotes cerebral microvasculogenesis without causing injury is essential.
Article
Cell Biology
Xiaoling Cui, Jie Wen, Nan Li, Xuxiao Hao, Shangli Zhang, Baoxiang Zhao, Xunwei Wu, Junying Miao
Summary: In this study, we identified a new inhibitor of PHD2 and demonstrated the new role of HOCI in cell differentiation. Moreover, we elucidated the mechanism by which the HOCI probe CPP induced the differentiation of HDFs into VECs.
Article
Ophthalmology
Xiaoling Shi, Nuo Dong, Qi Qiu, Shanhua Li, Jiaxing Zhang
Summary: The study demonstrates that salidroside (SAL) protects against hypoxia-induced human retinal microvascular endothelial cells (HRMECs) injury by regulating the miR-138/ROBO4 axis.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2021)
Article
Biochemistry & Molecular Biology
Bomin Son, Wesuk Kang, Soyoon Park, Dabin Choi, Taesun Park
Summary: Skin dermis aging is caused by reduced collagen synthesis, with olfactory receptors playing a role in cell survival and collagen synthesis. Among the studied ORs, OR51B5 stands out as a key player in cell survival and collagen synthesis.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Surgery
Yinhua Zhao, Yanyu Shi, Huang Lin
Summary: The study showed that co-culture with ADSCs could reduce apoptosis and enhance proliferation of HDMECs, with hypoxic preconditioning of ADSCs having a more pronounced effect. This approach increased vascular endothelial growth factor levels, demonstrating the potential to reduce I/R injury associated with skin flap transplantation surgery.
JOURNAL OF SURGICAL RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Sullim Lee, Yea Jung Choi, Chen Huo, Akida Alishir, Ki Sung Kang, Il-Ho Park, Taesu Jang, Ki Hyun Kim
Summary: Human skin is composed of the epidermis and dermis, which work together to maintain the skin's tensile strength. Skin aging, however, leads to the degradation of collagen and elastin fibers, resulting in wrinkles, pigmentation, and sagging. This study investigated natural products derived from ginkgo fruit that have protective effects against TNF-alpha-induced skin aging.
Article
Peripheral Vascular Disease
Carlotta Tacconi, Yuliang He, Luca Ducoli, Michael Detmar
Summary: Lymphatic and blood vascular endothelial cells exhibit distinct epigenetic features at the transcriptional level, with blood vascular lineage genes showing a more repressed histone composition in lymphatic ECs. DNA methylation at promoters has less impact on the differential transcriptomes of lymphatic versus blood vascular ECs. Meta-analyses identified BCL6 and MEF2C as potential transcriptional regulators governing endothelial lineage specificity.
Article
Biochemistry & Molecular Biology
Marta Karkoszka, Jakub Rok, Klaudia Banach, Justyna Kowalska, Zuzanna Rzepka, Dorota Wrzesniok
Summary: This study evaluated the influence of Meloxicam and UVAR on skin cell homeostasis. The results showed that co-treatment with Meloxicam and UVAR inhibited skin cell proliferation and caused morphological changes. Additionally, Meloxicam and UVAR disrupted redox homeostasis by lowering the intracellular level of reduced thiols. The study revealed that the phototoxic activity of Meloxicam is associated with oxidative stress induction and disruptions in cell homeostasis.
Article
Cell & Tissue Engineering
Edward X. Han, Hong Qian, Bo Jiang, Maria Figetakis, Natalia Kosyakova, George Tellides, Laura E. Niklason, William G. Chang
Summary: The Therapeutic Vascular Conduit (TVC) is a promising cell transplantation approach that utilizes an acellular vessel as a scaffold to prevent cell hypoxia. Implantation of TVCs containing erythropoietin-secreting cells can increase serum levels of erythropoietin and hemoglobin, but long-term efficacy is limited by macrophage infiltration and luminal obstruction.
NPJ REGENERATIVE MEDICINE
(2021)
Article
Cell Biology
Sandra Dienemann, Vanessa Schmidt, Tabea Fleischhammer, Julia H. H. Mueller, Antonina Lavrentieva
Summary: In vitro cultivation conditions are important for understanding cell physiology and responses to external stimuli. Oxygen concentrations are crucial in influencing cell behaviors in both 2D and 3D cultures. This study genetically modified two types of human endothelial cells and monitored their responses to different oxygen concentrations. The findings demonstrated different hypoxic signatures for these cells in both 2D and 3D culture systems.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Bairong Fang, Xiancheng Wang, Yang Sun, Xiang Xiong, Xianxi Meng, Wenbo Li, Zhongjie Yi
Summary: Flap expansion is an important method used in wound repair and organ reconstruction, but ischemic necrosis of distal skin flaps remains a complication. This study found that C-C motif chemokine ligand 2 (CCL2) and C-C motif chemokine receptor 2 (CCR2) were upregulated in reperfusion-exposed skin flap tissues. Hypoxia induced the expression of HIF-1 alpha and CCL2 in adipose-derived stem cells (ADSCs), which promoted HDMEC proliferation, migration, and tube formation. ADSCs transplantation improved flap survival and angiogenesis, and this effect was partially eliminated by CCL2 knockdown or enhanced by CCL2 overexpression in ADSCs.
JOURNAL OF PHYSIOLOGY AND BIOCHEMISTRY
(2023)