Determination of Glucose Using Pseudobienzyme Channeling Based on Sugar-Lectin Biospecific Interactions in a Novel Organic-Inorganic Composite Matrix

This work presents the synthesis of gold/platinum hybrid nanostructure supported on silica nanofibers (GP-SNFs) and then a novel strategy to fabricate pseudobienzyme channeling sensor based on sugar-lectin biospecific interactions for sensitive determination of glucose. First, prussian blue (PB) was deposited on the glassy carbon electrode as a redox probe. Then, a porous organic conducting polymer containing abundant amino groups (a derivative of 3,4,9,10-perylenetetracarboxylicdianhydride, was abbreviated as PTC-NH2) was coated on the surface of the PB film, which could not only prevent the leakage of the PB efficiently, but also provide an interface of abundant amino-groups to assemble GP-SNFs for the immobilization of concanavalin A (Con A). Finally, the glucose oxidase (GOD) was attached on the electrode surface through the strong biological affinity links between Con A and sugar chains intrinsically. Some important parameters of the sensor such as the catalytic rate constant (k(s)), the charge transfer coefficient (alpha), the surface coverage, and the apparent Michaelis-Menten constant (K-M(app)) were evaluated and discussed in detail. Furthermore, the proposed biosensor possessed high sensitivity and stability for the detection of glucose, and the linear response range from 3.0 x 10(-6) to 2.3 x 10(-3) M and the detection limit of 1.0 x 10(-6) M.