Article
Chemistry, Analytical
W. Cheuquepan, S. Hernandez, M. Perez-Estebanez, L. Romay, A. Heras, A. Colina
Summary: Electrochemistry can be used to generate SERS substrates, utilizing the plasmonic properties of nanos-tructures and the chemical mechanism, with time-resolved Raman spectroelectrochemistry providing valuable information. Comparing Au and Ag metals for folic acid detection in drugs, it was found that Ag SERS substrates outperformed Au SERS substrates in complex samples.
JOURNAL OF ELECTROANALYTICAL CHEMISTRY
(2021)
Article
Electrochemistry
Sheila Hernandez, Juan Perales-Rondon, Aranzazu Heras, Alvaro Colina
Summary: EC-SOERS is a new phenomenon that enhances the Raman signal during the oxidation of a metal surface. Studies show that carbonyl and carboxyl groups are necessary to observe this phenomenon, with a delocated charge being important factor. Evaluating the enhancement factors for different molecules with highly delocated charge and a carboxyl group sheds more light on the interaction of molecules with the electrochemically generated SOERS substrates.
ELECTROCHIMICA ACTA
(2021)
Article
Chemistry, Physical
Yuenan Pan, Qi Chu, Shuang Guo, Sila Jin, Eungyeong Park, Yeonju Park, Yantao Sun, Lei Chen, Young Mee Jung
Summary: The purpose of this study is to investigate the charge transfer process and SERS enhancement mechanism in the Ag/MBA/PAN system with varying thicknesses of PAN. The results show that increasing the PAN thickness leads to higher frequency and intensity of the characteristic MBA band, as well as increased intensity of the b2 mode. The degree of CT also increases with increasing PAN thickness, due to the excited SPR from Ag. The electromagnetic enhancement mechanism at the PAN/Ag interface generates hot electrons and facilitates electron transfer in the CT process.
APPLIED SURFACE SCIENCE
(2022)
Article
Chemistry, Analytical
Elodie Dumont, Charlotte De Bleye, Gilles Rademaker, Laureen Coic, Julie Horne, Pierre-Yves Sacre, Olivier Peulen, Philippe Hubert, Eric Ziemons
Summary: A new methodology combining solid SERS substrates, a protein filtering device, and a handheld Raman analyzer enables near real-time, non-invasive, and non-destructive SERS monitoring of biological samples. This method has shown promising versatility and the capability to reduce cellular stress and contamination hazards.
Article
Spectroscopy
Tong Zhang, Shunhua Zhu, Jingjing Wang, Zhiying Liu, Mingxin Wang, Shibao Li, Qingli Huang
Summary: Fabricating sensitive, stable and low-cost nanomaterials for glucose detection is crucial for disease diagnosis and monitoring. In this study, a nanocomposite (polypyrrole bridging GO@Au@MnO2) was developed as a novel surface-enhanced Raman scattering (SERS) nanoprobe for quantitative glucose detection in trace serum.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Review
Chemistry, Multidisciplinary
Ilya A. Milekhin, Alexander G. Milekhin, Dietrich R. T. Zahn
Summary: This study presents the optical response of spherical CdSe nanocrystals using SERS and TERS techniques, achieving significant enhancement of optical phonons by selecting appropriate plasmonic substrates. Combining Raman scattering with atomic force microscopy offers nanoscale spatial resolution for investigating phonon properties. Gap-mode TERS further enhances Raman scattering of CdSe NCs' optical phonon modes with nanometer spatial resolution, providing new pathways for optical characterization and phonon spectrum details at the nanometer scale of single semiconductor nanostructures.
Article
Physics, Multidisciplinary
Guanzhou Lin, Bingquan Yang, Chenglong Zhang, Jia Zhu, Yang Wang, Liye Li, Shisong Xiong, Jinwen Zhang, Zhimei Qi, Wengang Wu
Summary: In this study, a simple composite substrate combining a metal metasurface and silver nanoparticles was developed to enhance the performance of surface-enhanced Raman scattering (SERS) sensing. The composite substrate showed high enhancement factor, good stability, and reproducibility, enabling the detection of trace substances.
Article
Chemistry, Multidisciplinary
Myoung Gyu Kim, Miyeon Jue, Kwan Hee Lee, Eun Yeong Lee, Yeonjeong Roh, Minju Lee, Hyo Joo Lee, Sanghwa Lee, Huifang Liu, Bonhan Koo, Yoon Ok Jang, Eui Yeon Kim, Qiao Zhen, Sung-Han Kim, Jun Ki Kim, Yong Shin
Summary: The study introduces a deep learning assisted ZnO-Au-SERS-based direct amplification (ZADA) system for rapid, sensitive molecular diagnostics. The system can amplify target nucleic acids directly on the SERS surface, eliminating the need for post-amplification hybridization and Raman reporters. Clinical validation with COVID-19 samples yielded high sensitivity and specificity. The integration of a deep learning program further enhanced sensitivity and specificity to 100%.
Article
Spectroscopy
Soogeun Kim, Young Jin Kim, Ayoung Bang, Wansun Kim, Samjin Choi, Hee Joo Lee
Summary: The study investigated the impact of Raman excitation wavelengths on the surface-enhanced Raman spectroscopy (SERS) for the identification of nontuberculous mycobacteria (NTM). By comparing SERS spectra with three commonly used excitation wavelengths, it was found that NTM species could be distinguished with statistical differences. Utilizing principal components-linear discriminant analysis and leave-one-out cross validation, the study achieved identification accuracies for six NTM species using aromatic amino acid biomarkers at different excitation wavelengths.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Weixing Gao, Weiman Duan, Dandan Peng, Juan Li, Zhangmei Hu, Dongmei Wang, Zhengjun Gong, Meikun Fan
Summary: The complexity of substances in environmental water bodies is leading to severe ecotoxicological problems. Evaluating water quality accurately based on the species and concentration of chemicals alone is difficult due to the intricate interaction among various chemicals. Therefore, the acute toxicity evaluation of water is of significant importance in environmental monitoring, especially in emergency situations. This study proposes a SERS approach to evaluate the acute toxicity of fresh water for the first time, using indole as an indicator. It demonstrates the feasibility of using SERS-based microbial sensors to assess acute toxicity in water.
MICROCHEMICAL JOURNAL
(2023)
Article
Materials Science, Multidisciplinary
Junxiang Xiang, Yunxia Wang, Yupeng Wu, Qi Peng, Langquan Shui, Wengen Ouyang, Tao Ding, Ze Liu
Summary: A cost-effective approach for fabricating highly ordered metallic pillar arrays in centimeter scale has been developed using superplastic nanomolding and anodic aluminum oxide templates. This method enables surface-enhanced Raman scattering with an enhancement factor of approximately 10^6 and provides guidance for quantitative analysis on the sub-micrometer scale.
ADVANCED MATERIALS TECHNOLOGIES
(2022)
Review
Chemistry, Analytical
Dan Li, Ling Xia, Gongke Li
Summary: This paper reviews the classification and applications of nanozymes in surface-enhanced Raman scattering (SERS), highlighting their importance in disease diagnosis, food safety, and environmental safety. The practical shortcomings of nanozymes in SERS applications are also discussed, and suggestions for further research are provided.
Article
Spectroscopy
Mengmeng Zheng, Yamin Lin, Jianshu Xu, Jiamin Gao, Wei Gong, Shusen Xie, Yun Yu, Juqiang Lin
Summary: This study compared electroporation-assisted and ultrasound-assisted delivery methods for surface-enhanced Raman scattering (SERS) detection, finding that the two methods have different capabilities in delivering nanoparticles into living cells. Ultrasound-assisted SERS showed better sensitivity, specificity, and accuracy in discriminating and identifying drug-treated degranulated mast cells compared to electroporation-assisted SERS.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2022)
Review
Chemistry, Physical
Ryan D. Norton, Hoa T. Phan, Stephanie N. Gibbons, Amanda J. Haes
Summary: This review discusses the recent advances in quantitative SERS detection, focusing on intermolecular interactions, surface selection rules, and target molecule solubility and accessibility. The impacts of surface selection rules and enhancement mechanisms on the activation and deactivation of normal Raman modes in SERS are explored. The review also summarizes the experimental conditions that can be used to tune the molecular affinity to and density near SERS substrates.
ANNUAL REVIEW OF PHYSICAL CHEMISTRY
(2022)
Article
Chemistry, Applied
Jie He, Hongxia Li, Lulu Zhang, Xiao Zhi, Xin Li, Xin Wang, Zijian Feng, Guangxia Shen, Xianting Ding
Summary: This study demonstrated an easy-prepared and low-cost surface enhanced Raman Scattering (SERS) chip for the quantitative testing of carbendazim in Chinese tea, which offers more aggregation-induced hotspots, strong 3D synergetic effects, and reduces costs without impacting performance.
Article
Instruments & Instrumentation
Xinchen Deng, Kirsty Milligan, Ramie Ali-Adeeb, Phillip Shreeves, Alexandre Brolo, Julian J. Lum, Jeffrey L. Andrews, Andrew Jirasek
Summary: Raman spectroscopy, when combined with GBR-NMF-RF framework, can be a powerful tool in monitoring radiation response profiles and biochemical dynamics in different molecular histotypes of irradiated breast cancer cells. The framework yields high accuracy, sensitivity, and specificity in classifying molecular histotypes and assessing the importance of Raman biochemical basis spectra for each classification test. High contributions from glycogen and lipids were found to be important in molecular histotype classifications.
APPLIED SPECTROSCOPY
(2022)
Article
Chemistry, Physical
Nathan C. Lindquist, Ariadne Tuckmantel Bido, Alexandre G. Brolo
Summary: This paper investigates the mechanism of fluctuations in Surface Enhanced Raman Spectroscopy (SERS) signals and finds that the underlying source of these fluctuations is a complex interplay of different effects. High-speed SIF activity is present in both dry and wet nanoparticle environments, and a simple model is proposed to explain the contributions to the fluctuations.
JOURNAL OF PHYSICAL CHEMISTRY C
(2022)
Article
Chemistry, Analytical
Pearl Thompson, Ana Livia de Carvalho Bovolato, Gisela Ibanez-Redin, Alexandre G. Brolo
Summary: A rapid lateral flow screening tool using saliva samples and cell phone quantification has been developed, providing sensitive detection of IgG antibodies against SARS-CoV-2 within 10 minutes.
Article
Chemistry, Analytical
Ariadne Tuckmantel Bido, Alexandre G. Brolo, Arash Azarakhshi
Summary: Surface-enhanced Raman scattering (SERS) is a sensitive spectroscopic technique, but its reproducibility has been an issue in analytical chemistry applications. This study demonstrates that reliable quantification of SERS immunoassay at low concentrations is possible with careful experimental design and appropriate data analysis statistics.
ANALYTICAL CHEMISTRY
(2022)
Article
Chemistry, Multidisciplinary
Minh-Hai Tran, Ian Booth, Bruno G. daFonseca, Peter Berrang, Jeremy E. Wulff, Alexandre G. Brolo
Summary: An economical and facile method has been developed to synthesize a precursor for carbon films and materials. This precursor can be easily coated onto substrates and converted into a graphitic-like structure after mild thermal treatment. This method allows for the coating of glass surfaces of different shapes and forms.
Article
Chemistry, Multidisciplinary
Makayla M. Schmidt, Emily A. Farley, Marit A. Engevik, Trey N. Adelsman, Ariadne Tuckmantel Bido, Nathan D. Lemke, Alexandre G. Brolo, Nathan C. Lindquist
Summary: The concept of plasmonic hotspots is central to nanophotonics, specifically in surface-enhanced Raman scattering (SERS). SERS signals from single molecules often exhibit fluctuations, challenging the concept of static hotspots. Recent experiments have shown SERS intensity fluctuations (SIFs) to occur over a wide range of timescales due to various physical mechanisms causing SERS and the dynamic nature of light-matter interaction. This study presents a high-speed acquisition system that captures SERS spectra with microsecond time resolution, revealing that high-speed SIF events occur with relatively equal probability over a broad spectral range.
Article
Instruments & Instrumentation
Kirsty Milligan, Kendra Scarrott, Jeffrey L. Andrews, Alexandre G. Brolo, Julian J. Lum, Andrew Jirasek
Summary: Raman spectroscopy is a useful tool for obtaining biochemical information, but interpreting the data can be challenging. Our group has demonstrated a non-negative matrix factorization framework as an alternative to dimensionality reduction techniques for Raman spectroscopy data related to radiation response monitoring. We evaluated the accuracy of the model in reconstructing mixture solutions of known concentrations and considered factors such as spectral bases, noise thresholds, and different biochemical groups. Overall, the model performed well and solid bases spectra were comparable to solution bases spectra. The inclusion of an unconstrained component did not significantly affect the deconstruction, as long as all biochemicals were included in the model.
APPLIED SPECTROSCOPY
(2023)
Article
Chemistry, Analytical
Moyra F. Vieira, Ana Livia de Carvalho Bovolato, Bruno G. da Fonseca, Celly M. S. Izumi, Alexandre G. Brolo
Summary: A biosensor was developed for directly detecting human immunoglobulin G (IgG) and adenosine triphosphate (ATP) using gold nanoparticles/polystyrene-b-poly(2-vinylpyridine) (AuNP/PS-b-P2VP) nanocomposites. The substrates were functionalized with carboxylic acid groups to bind anti-IgG and anti-ATP and detect IgG and ATP. SEM images showed gold nanoparticles adsorbed onto a porous thin film. UV-VIS and SERS were used to characterize substrate functionalization and antibody-target interaction. PCA discriminated between samples before and after affinity tests. The biosensor was sensitive to IgG concentrations with a limit of detection of 1 μg/mL.
Article
Chemistry, Multidisciplinary
Tiago S. Pacheco, Zelia M. C. Ludwig, Victor H. Oliveira, Ingrid D. Barcelos, Rafael L. de Souza, Edinei C. Paiva, Maximiliano D. Martins, Flavia C. Marques, Gustavo F. S. Andrade, Santunu Ghosh
Summary: In this study, we synthesized and studied the spectroscopic properties of (NH4)2(SO4)2Y(H2O)6 (Y = Ni, Mg) crystals doped with AgNO3 or H3BO3. The effects of dopants on the vibrational modes of the ligands NH4 and SO4, complexes Mg(H2O)6 and Ni(H2O)6, and H2O molecules present in these crystals were investigated using Raman and infrared spectroscopies. The presence of Ag and B dopants and their influence on the crystal lattice were identified through band analysis. Thermogravimetric measurements showed an increase in the initial temperature of crystal degradation due to the presence of dopants. Raman spectroscopy of the crystal residues after thermogravimetric measurements provided insights into the degradation processes occurring after crystal pyrolysis.
Article
Spectroscopy
Bruno G. daFonseca, Sapanbir S. Thind, Ian Booth, Alexandre G. Brolo
Summary: Principal component analysis (PCA) and linear discriminant analysis (LDA) were used to classify different types of carbon material based on their Raman spectra, providing an avenue for automated classification of carbon materials using Raman spectroscopy and machine learning.
JOURNAL OF RAMAN SPECTROSCOPY
(2023)
Article
Chemistry, Multidisciplinary
Minh-Hai Tran, Ian Booth, Arash Azarakhshi, Peter Berrang, Jeremy Wulff, Alexandre G. Brolo
Summary: This paper introduces a new method for large-scale synthesis of graphene that minimizes the creation of structural defects. The method involves hydrogen functionalization of graphite followed by thermal dehydrogenation, and it is demonstrated to be effective for the preparation of high-quality graphene films.
Article
Chemistry, Analytical
Ariadne Tuckmantel Bido, Katherine J. I. Ember, Dominique Trudel, Madeleine Durand, Frederic Leblond, Alexandre G. Brolo
Summary: The SARS-CoV-2 pandemic has posed a challenge in containing the spread of the virus, especially in low and middle-income countries due to limited access to screening tests. In this study, a low-cost biosensor device based on localized surface plasmon resonance (LSPR) was developed for the screening of SARS-CoV-2. The sensor showed high sensitivity and specificity in detecting the virus in saliva samples, providing a potential solution for screening challenges in underserved communities.
ANALYTICAL METHODS
(2023)
Article
Chemistry, Multidisciplinary
Makayla M. Schmidt, Emily A. Farley, Marit A. Engevik, Trey N. Adelsman, Ariadne Tuckmantel Bido, Nathan D. Lemke, Alexandre G. Brolo, Nathan C. Lindquist
Summary: The concept of plasmonic hotspots plays a crucial role in the field of nanophotonics, particularly in surface-enhanced Raman scattering (SERS). Recent experiments have shown that these hotspots exhibit significant fluctuations at different timescales, which are likely caused by the complex interplay of multiple effects. This study presents a high-speed acquisition system that captures the dynamic processes of SERS fluctuations and finds that these fluctuations occur over a wide spectral range.
Article
Chemistry, Physical
Flavia C. Marques, Raisa Siqueira Alves, Diego Pereira dos Santos, Gustavo F. S. Andrade
Summary: The present study reports a direct approach to generate efficient hot spots using a nonresonant molecule bound to the inner part or hot spots that can increase the SERS sensitivity to obtain spectra of one and a few molecules. The study also investigates the self-assembly process of AgNP triggered by the 4-MBA adsorbate bonded to the Ag surface, and explores the SERS intensification of 4-MBA in low concentrations.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2022)
Article
Chemistry, Analytical
Xinchen Deng, Kirsty Milligan, Alexandre Brolo, Julian J. Lum, Jeffrey L. Andrews, Andrew Jirasek
Summary: The use of Raman spectroscopy combined with a data analytical framework allows for the monitoring of biochemical responses and spatial correlation with hypoxic regions in radiation therapy. This provides a deeper understanding of individual biochemical dynamics in radiation responses, which can be applied in cancer therapy.
