Journal
JOURNAL OF PHYSICAL CHEMISTRY C
Volume 112, Issue 49, Pages 19163-19167Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp806649a
Keywords
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Funding
- U.S. Army Research Office [W911NF-07-1-0409]
- NTH [EB-001307, GM85555]
- Duke GPNANO
- NSF (IGERT [DGE-0221632]
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A simple, practical, and model-free method is presented, by which kinetic data can be extracted from variable loading-rate single molecule dynamic force spectroscopy experiments. The constant sampling rate of digital acquisition facilitates the sorting of multiple force curves into histograms that reflect the collective force-time history of the bonds of interest. Combining the force-time history with similar histograms of force-induced events, in particular bond rupture, gives a direct measure of rate vs applied force. The utility of the method is demonstrated through its application to simulated data.
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