Article
Chemistry, Multidisciplinary
Yuji Takaoka, Shohei Esaki, Koichi Sakaguchi, Tomotsumi Fujisawa, Masashi Unno, Shintaro Morisada, Keisuke Ohto, Hidetaka Kawakita
SEPARATION SCIENCE AND TECHNOLOGY
(2020)
Review
Biochemistry & Molecular Biology
Takayuki Shimizu, Shinji Masuda
JOURNAL OF BIOCHEMISTRY
(2020)
Article
Chemistry, Physical
Chihiro Azai, Jiro Harada, Shogo Fujimoto, Shinji Masuda, Daisuke Kosumi
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Haruya Inago, Ryoichi Sato, Shinji Masuda
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2020)
Article
Multidisciplinary Sciences
Mai Duy Luu Trinh, Daichi Miyazaki, Sumire Ono, Jiro Nomata, Masaru Kono, Hiroyuki Mino, Tatsuya Niwa, Yuki Okegawa, Ken Motohashi, Hideki Taguchi, Toru Hisabori, Shinji Masuda
Summary: The study identified a chloroplast protein named TCR, which is involved in electron flow downstream of PSI, contributing to P700 oxidation. Loss of TCR resulted in significant growth reduction in the Arabidopsis pgr5-tcr double mutant, with abnormal oxidation and reduction in the plastoquinone pool.
Article
Plant Sciences
Mina Goto, Akira Oikawa, Shinji Masuda
Summary: The Arabidopsis ppGpp-overproducing mutant shows a larger biomass than wild type under nitrogen-limiting conditions by modulating amino-acid metabolism. The regulatory nucleotide ppGpp controls gene expression and enzyme activities, but the mechanisms behind its influence on plant growth and biomass are still unclear. The accumulation of ppGpp affects amino-acid and protein homeostasis, facilitating growth under nitrogen-limiting conditions.
Article
Plant Sciences
Mai Duy Luu Trinh, Akira Hashimoto, Masaru Kono, Shinichi Takaichi, Yoichi Nakahira, Shinji Masuda
Summary: Research has shown that controlling pH homeostasis in the chloroplast is crucial for regulating photosynthesis and other metabolic processes in plants. By studying ycf10 tobacco mutants, it was found that ycf10 may play a role in light-induced optimization of plastidial pH, which is important for photosynthesis.
Article
Plant Sciences
Kazuhiro Ito, Doshun Ito, Mina Goto, Sae Suzuki, Shinji Masuda, Koh Iba, Kensuke Kusumi
Summary: Guanosine tetraphosphate (ppGpp) is involved in regulating chloroplast gene expression in plants, affecting photosynthesis and plant growth. In rice, ppGpp inhibits GTP biosynthesis enzyme GKpm, and overexpression of ppGpp synthase RSH3 enhances chlorosis and lowers photosynthetic rates. The basal ppGpp level in rice during leaf development appears to be determined by the opposing activities of multiple RSH enzymes.
PLANT AND CELL PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kasane Suzuki, Shinji Masuda
Summary: The control of pH in chloroplasts is crucial for regulating photosynthesis, but the specific regulatory mechanisms of H+ homeostasis in chloroplasts are not fully understood. Recent research suggests that the cyanobacterial PxcA homolog DLDG1 is involved in controlling plastidial pH. By examining various double mutants lacking specific non-photochemical quenching (NPQ)-related proteins, it was found that PsbS functions upstream of DLDG1, PGR5 affects NPQ independently, and FLAP1 and DLDG1 regulate ΔpH independently.
Article
Plant Sciences
Masataka Inazu, Takanari Nemoto, Yuto Omata, Sae Suzuki, Sumire Ono, Yuri Kanno, Mitsunori Seo, Akira Oikawa, Shinji Masuda
Summary: ppGpp functions as a secondary messenger in bacteria and chloroplasts, impacting gene expression, metabolism, and plant defense.
PLANT AND CELL PHYSIOLOGY
(2023)
Article
Multidisciplinary Sciences
Takayuki Shimizu, Tomoaki Ida, Giuliano T. Antelo, Yuta Ihara, Joseph N. Fakhoury, Shinji Masuda, David P. Giedroc, Takaaki Akaike, Daiana A. Capdevila, Tatsuru Masuda
Summary: Sulfide plays crucial roles in physiological activities, and recent studies have shown its endogenous generation and metabolism into polysulfides inside cells. However, the relationship between polysulfide metabolism and sensing mechanisms is not well understood. This study investigates the role of polysulfide-metabolizing enzyme SQR on cellular polysulfide speciation and transcriptional regulation in Rhodobacter capsulatus. The results reveal a novel association between polysulfide metabolism and sensing mechanisms inside cells.
Article
Biotechnology & Applied Microbiology
Yuki Sugimoto, Shinji Masuda
Summary: Phototaxis is a phenomenon where cyanobacteria move towards a light source, controlled by PixD and PixE in cyanobacterium. PixE influences the direction of movement and localizes in protein complexes in membrane fractions, controlling the activity of PilB1 to regulate pilus motility.
JOURNAL OF GENERAL AND APPLIED MICROBIOLOGY
(2021)
Article
Plant Sciences
Takayuki Shimizu, Yuuki Hayashi, Munehito Arai, Shawn E. McGlynn, Tatsuru Masuda, Shinji Masuda
Summary: The study revealed that SqrR can bind heme, reducing its DNA-binding affinity and altering its regulatory activity. Changes in intracellular heme concentration are associated with a significant reduction in SqrR-mediated transcriptional repression.
PLANT AND CELL PHYSIOLOGY
(2021)
Article
Biology
Doshun Ito, Hinata Kawamura, Akira Oikawa, Yuta Ihara, Toshio Shibata, Nobuhiro Nakamura, Tsunaki Asano, Shun-Ichiro Kawabata, Takashi Suzuki, Shinji Masuda
COMMUNICATIONS BIOLOGY
(2020)
Article
Plant Sciences
Annik Jakob, Hiroshi Nakamura, Atsuko Kobayashi, Yuki Sugimoto, Annegret Wilde, Shinji Masuda
PLANT AND CELL PHYSIOLOGY
(2020)