Article
Biotechnology & Applied Microbiology
George V. Sharonov, Oksana V. Nekrasova, Ksenia S. Kudryashova, Mikhail P. Kirpichnikov, Alexey V. Feofanov
Summary: Developing targeted therapy requires screening drug candidates for their affinity and selectivity for a certain binding site, which is achieved through a screening assay for receptor binding using a hybrid channel tagged with a fluorescent protein. By transforming E. coli into spheroplasts, the channel becomes accessible for soluble compounds, enabling the evaluation of Kv channel blockers for high throughput screening. This method offers potential for multiplexing, scaling and automation, and shows promising results in measuring binding constants and displacement of fluorescently labeled blockers.
BIOENGINEERING-BASEL
(2021)
Article
Chemistry, Physical
Brankica Jankovic, Jeannette Ruf, Claudio Zanobini, Olga Bozovic, David Buhrke, Peter Hamm
Summary: The phototriggered unbinding of the intrinsically disordered S-peptide from the RNase S complex was studied using transient IR spectroscopy across a wide range of time scales. The experiment revealed a full sequence of events including unfolding of the helical structure of the S-peptide, peptide unbinding, and structural response of the S-protein. The binding mechanism of S-peptide was classified as induced fit, while the structural response of the S-protein was better described as conformational selection.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemistry & Molecular Biology
Rym ElFessi, Oussema Khamessi, Najet Srairi-Abid, Jean-Marc Sabatier, Jan Tytgat, Steve Peigneur, Riadh Kharrat
Summary: Bot33 is a new short polypeptide purified from the venom of Buthus occitanus tunetanus scorpion. It has potential therapeutic effects and displays a different mode of action on ion channels compared to other scorpion toxins, which is worth further exploration.
Article
Chemistry, Multidisciplinary
Zhaowen Dong, J. Terence Blaskovits, Farzaneh Fadaei-Tirani, Rosario Scopelliti, Andrzej Sienkiewicz, Clemence Corminboeuf, Kay Severin
Summary: The study found that mesoionic imidazolylidenes bearing a diphenyltriazinyl (Dpt) substituent exhibit improved pi-acidic properties compared to Arduengo-type N-heterocyclic carbenes. This discovery was supported by computational analysis and experimental evidence. The new carbene was utilized to stabilize a low-valent paramagnetic tin compound, demonstrating its usefulness.
CHEMISTRY-A EUROPEAN JOURNAL
(2021)
Article
Chemistry, Multidisciplinary
Carlos de la Cruz, Roberto Sanz, Anisley Suarez, Edgar Ventosa, Rebeca Marcilla, Andreas Mavrandonakis
Summary: This article utilizes density functional theory calculations to screen around 100 phenazine derivatives in aqueous media and identifies crucial positions for functionalization with multiple hydroxy groups to design new anolytes. The combination of experimental and computational methods guides the development of a new molecule with a record low reversible redox potential as a potential anolyte for aqueous redox flow batteries.
Article
Mechanics
Yuan-Heng Zhang, Huan-Feng Duan, Xu-Feng Yan, Alessandro Stocchino
Summary: This paper presents an experimental study on the impact of variable solid volume fractions and aspect ratios on the flow behavior and wake structure of a centre-channel rectangular porous patch. The study establishes the importance of aspect ratio in wake vortex generation. By elongating the patch, a vortex street can be generated due to the promotion of transverse velocity gradient in the wake region. The paper also proposes non-dimensional threshold maps to express the combined effects of solid volume fraction and aspect ratio on the initiation of the wake vortex street.
JOURNAL OF FLUID MECHANICS
(2023)
Article
Chemistry, Multidisciplinary
Wentian Zou, Liuzhou Gao, Jia Cao, Zhenxing Li, Guoao Li, Guoqiang Wang, Shuhua Li
Summary: The mechanistic details of the hydroboration reactions of imines and the efficient catalysis of BH3 . SMe2 for these reactions have been investigated through computational and experimental studies.
CHEMISTRY-A EUROPEAN JOURNAL
(2022)
Article
Energy & Fuels
Laleh Rezaeian Kouchi, Seyyed Mohammad Ghoreishi, Mohammad Reza Rahimpour
Summary: This research aimed to simulate the CO2 capture efficiency and flux from a gas stream using aqueous potassium glycinate and potassium glutaminate solutions in a rotating liquid sheet contactor. The study found that using potassium glycinate solution could achieve a separation efficiency of over 95%, with an increase in gas flow rate leading to higher CO2 capture.
Article
Biochemistry & Molecular Biology
Lukas Petera, Klaudia Mrazikova, Lukas Nejdl, Kristyna Zemankova, Marketa Vaculovicova, Adam Pastorek, Svatopluk Civis, Petr Kubelik, Alan Heays, Giuseppe Cassone, Jiri Sponer, Martin Ferus, Judit Sponer
Summary: The research demonstrates that thymine can be synthesized from formamide in the presence of catalysts, and the presence of formic acid can also facilitate the conversion of uracil to thymine in the absence of catalysts. Experimental results and quantum chemical modeling support the feasibility of this reaction mechanism.
Article
Materials Science, Multidisciplinary
Felix Hehnen, Gabi Wendt, Jens Schaller, Paul Geus, Joachim Villwock, Ulrich Kertzscher, Leonid Goubergrits
Summary: CECs, as reliable biomarkers for cardiovascular diseases, face challenges in detection reliability. To address this issue, a BMProbe for in vivo isolation of CECs is proposed, with experimental and numerical studies conducted to investigate cell attachment under different flow conditions.
ADVANCED ENGINEERING MATERIALS
(2022)
Article
Materials Science, Ceramics
Davide Lenaz, Stefano Giovanoni, Francesco Parrino, Danilo Bersani, Filippo Parisi
Summary: This study aims to investigate the evolution of glauconites with different levels of maturity. Tests were conducted on glauconitic grains from the Belluno basin in Italy using electron microprobe, Raman, and Thermogravimetric analysis. The results show that Raman spectra and TGA measurements are sensitive to the maturity level of glauconites.
CERAMICS INTERNATIONAL
(2023)
Article
Chemistry, Physical
Zhenghai Yang, Bing-Jian Sun, Chao He, Shane Goettl, Yu-Ting Lin, Agnes H. H. Chang, Ralf Kaiser
Summary: This study explores the reaction dynamics of silicon hydrides using crossed molecular beam techniques, providing insights into the chemical bonding properties and isomerization processes of silicon hydrides at the molecular level.
JOURNAL OF PHYSICAL CHEMISTRY A
(2021)
Article
Chemistry, Multidisciplinary
Lilian Bossuet, El Mehdi Benhani
Summary: This paper explores the feasibility and potential threats of inside-SoC cache attacks using the cache coherency mechanism of a complex heterogeneous SoC-FPGA, providing proof of two cache timing attacks and a cache-based covert channel for hidden communication.
APPLIED SCIENCES-BASEL
(2021)
Review
Biochemistry & Molecular Biology
Germana Cocozza, Stefano Garofalo, Riccardo Capitani, Giuseppina D'Alessandro, Cristina Limatola
Summary: The research interest in microglia's role in neurodegenerative diseases is increasing rapidly, with a focus on the importance of potassium channels in modulating microglial functions in diseases like Alzheimer's, Parkinson's, and ALS. The discovery of specific biomarkers of microglia phenotype may have wide applications in human diseases and could lead to tailored therapeutic interventions.
Article
Environmental Sciences
Bestami Tasar, Fatih Unes, Ercan Gemici, Martina Zelenakova
Summary: Regulation structures such as submerged vane are necessary to mitigate environmental damage caused by increased flooding in rivers. This study experimentally investigated the effect of 3-array submerged vane structures on flow velocities near the outer bank. Results from computational fluid dynamics (CFD) were verified and found to closely match the experimental results. The 3-array submerged vane with a 3-vane structure reduced flow velocities by 16-27% behind the vane, and the mean velocity along the depth decreased by 14-21%.
Article
Biochemistry & Molecular Biology
Daniela Glatzova, Harsha Mavila, Maria Chiara Saija, Tomas Chum, Lukasz Cwiklik, Tomas Brdicka, Marek Cebecauer
Summary: The research found that proline and glycine residues in the transmembrane segment of LAT can affect its localization and function by inducing a kink in the helix, and further reduce dependence on palmitoylation by replacing them with aliphatic amino acids.
Article
Biochemistry & Molecular Biology
Adela Tiffner, Romana Schober, Carmen Hoeglinger, Daniel Bonhenry, Saurabh Pandey, Victoria Lunz, Matthias Sallinger, Irene Frischauf, Marc Fahrner, Sonja Lindinger, Lena Maltan, Sascha Berlansky, Michael Stadlbauer, Rainer Schindl, Rudiger Ettrich, Christoph Romanin, Isabella Derler
Summary: This study demonstrates that the opening of Orai1 channel is regulated by a series of gating checkpoints, with locally induced changes spreading omnidirectionally within the complex. Loss-of-function mutations were found to dominate over gain-of-function mutations within and adjacent to the Orai subunit. Furthermore, salt-bridge interactions between Orai subunits were identified as a core element for channel opening.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2021)
Article
Immunology
Azadeh Nikouee, Matthew Kim, Xiangzhong Ding, Yuxiao Sun, Qun S. Zang
Summary: The study demonstrated that activation of Beclin-1 improves outcomes in pneumonia-induced sepsis by enhancing autophagy, reducing systemic infection and inflammation, and improving lung pathology. This suggests that targeting Beclin-1 activation may have therapeutic potential for sepsis.
FRONTIERS IN CELLULAR AND INFECTION MICROBIOLOGY
(2021)
Article
Chemistry, Physical
Mirza Wasif Baig, Marek Pederzoli, Mojmir Kyvala, Lukasz Cwiklik, Jiri Pittner
Summary: Halogenated and alkylated BODIPY derivatives are studied as potential photosensitizers for photodynamic therapy. Results show that derivatives containing bromine atoms have stronger spin-orbit couplings, although they are similar to alkylated derivatives at local minima. Both bromine atoms and alkyl moieties reduce the HOMO/LUMO gap, aiding in the efficiency of the photosensitizers.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Chemistry, Physical
Petr Pospisil, Lukasz Cwiklik, Jan Sykora, Martin Hof, Gregory M. Greetham, Michael Towrie, Antonin Vlcek
Summary: The excited-state properties and dynamics of two 6-(dimethylamino)-2-acylnaphthalene dyes were studied using picosecond time-resolved IR spectroscopy in solvents of different polarity. The results showed a transition from an initial excited state to a distinct structure in polar/H-bonding solvents, indicating a significant role in observing dynamic fluorescence Stokes shift.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Cell Biology
Matthew Kim, Azadeh Nikouee, Yuxiao Sun, Qing-Jun Zhang, Zhi-Ping Liu, Qun Sophia Zang
Summary: The study suggests that the Parkin/PINK1 mitophagy pathway is involved in the regulation of cardiac mitochondria-associated membranes (MAMs) during endotoxemia. However, the W402A point mutation is not sufficient to alleviate endotoxemia-induced cardiomyopathy.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Kamila Riedlova, Tereza Dolejsova, Radovan Fiser, Lukasz Cwiklik
Summary: This study investigates the mechanism of action of bio-inspired peptides originating from colicins on bacterial membranes using a combination of molecular simulations and experiments. The results show that these peptides are stable in lipid membranes and can induce membrane defects, water permeation, and membrane poration. Experimental assays confirm the ability of the H1 peptide to disrupt membranes. These findings are important for understanding the antimicrobial mechanisms of natural bacteriocins and developing small bio-inspired peptides that can disrupt bacterial membranes.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOMEMBRANES
(2022)
Article
Biochemistry & Molecular Biology
Martin Schicht, Kamila Riedlova, Mercedes Kukulka, Wenyue Li, Aurelius Scheer, Fabian Garreis, Christina Jacobi, Friedrich Paulsen, Lukasz Cwiklik, Lars Braeuer
Summary: The ocular surface requires a stable tear film and local immune defense mechanisms to protect the eye. This study focuses on surfactant protein G (SP-G) and its potential role in wound healing and reducing tear film surface tension.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cardiac & Cardiovascular Systems
Matthew Kim, Azadeh Nikouee, Raymond Zou, Di Ren, Zhibin He, Ji Li, Lu Wang, Danijel Djukovic, Daniel Raftery, Hayley Purcell, Daniel Promislow, Yuxiao Sun, Mohammad Goodarzi, Qing-Jun Zhang, Zhi-Ping Liu, Qun Sophia Zang
Summary: This study demonstrates that TB-peptide, through activating Beclin-1, provides cardiac protection against endotoxemia in both young and aged populations. Metabolomics analysis suggests that the protective effect of TB-peptide, independent of age, is associated with reprogramming of energy production via glucose and amino acid metabolisms.
JOURNAL OF THE AMERICAN HEART ASSOCIATION
(2022)
Article
Pharmacology & Pharmacy
Kamila Riedlova, Maria Chiara Saija, Agnieszka Olzynska, Piotr Jurkiewicz, Philippe Daull, Jean-Sebastien Garrigue, Lukasz Cwiklik
Summary: Benzalkonium chloride (BAK) compounds are commonly used as preservatives and stabilizers in topical ophthalmic products. In chronic eye conditions, the adverse effects of BAKs can accumulate, making preservative-free eye drops preferable. However, certain longer-chain BAKs, like cetalkonium chloride, have therapeutic functions in promoting wound healing and tear film stability.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Nanoscience & Nanotechnology
Paulina Chytrosz-Wrobel, Monika Golda-Cepa, Piotr Kubisiak, Waldemar Kulig, Lukasz Cwiklik, Andrzej Kotarba
Summary: The therapeutic function of biomaterial surface can be improved by the controlled release of biologically active molecules. Ultrasonic-assisted formation of nanoparticles with controlled size and morphology can be used for such functionalization. This study reported the synthesis of fluorouracil nanoparticles, a bioactive molecule used in anticancer therapy, and revealed the molecular-level picture of the cavitation bubble interface where the enrichment of fluorouracil molecules takes place. The proposed mechanism can be applied to other biologically relevant molecules, suggesting that the sonochemical method can be used for the controlled formation of their nanoparticles. The results indicate the potential practical implications of tailoring the surface of polymeric biomaterials via the embedment of nanoparticles as drug delivery systems.
ACS APPLIED NANO MATERIALS
(2023)
Article
Pharmacology & Pharmacy
Kamila Riedlova, Maria Chiara Saija, Agnieszka Olzynska, Katarina Vazdar, Philippe Daull, Jean-Sebastien Garrigue, Lukasz Cwiklik
Summary: The tear film lipid layer (TFLL) may serve as a reservoir for latanoprost, facilitating its prolonged release and potentially optimizing glaucoma treatment. These findings have significant implications for the development of new drug delivery systems targeting the TFLL.
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2023)
Article
Materials Science, Biomaterials
Paulina Chytrosz-Wrobel, Monika Golda-Cepa, Kamil Drozdz, Jakub Rysz, Piotr Kubisiak, Waldemar Kulig, Monika Brzychczy-Wloch, Lukasz Cwiklik, Andrzej Kotarba
Summary: This study investigates the effect of oxygen plasma treatment on the physicochemical properties of polyurethane surfaces and characterizes the polyurethane-water interfaces in detail through experiments and molecular dynamics simulations.
ACS BIOMATERIALS SCIENCE & ENGINEERING
(2023)
Meeting Abstract
Biophysics
Agnieszka Olzynska, Hanna Korolainen, Ilpo Vattulainen, Waldemar Kulig, Lukasz Cwiklik
BIOPHYSICAL JOURNAL
(2022)
Meeting Abstract
Biophysics
Maria Chiara Saija, Lukasz Cwiklik
BIOPHYSICAL JOURNAL
(2022)