Journal
JOURNAL OF PHYSICAL CHEMISTRY B
Volume 114, Issue 30, Pages 9912-9919Publisher
AMER CHEMICAL SOC
DOI: 10.1021/jp104167h
Keywords
-
Categories
Funding
- Fundacaopara a Ciencia e a Tecnologia (FCT), Portugal [POCI/QUI/56585/04, POCI/BIA-PRO/57263/04, SRFH/BPD/27128/2006, SRFH/BPD/44428/2008]
- European Commission [QLK3-CT-2000-00640]
Ask authors/readers for more resources
Thermal folding/unfolding kinetics of wild-type ubiquitin (wt-UBQ) was studied in a wide time range, from microseconds to seconds, by combining rapid-mixing T-jump and laser T-jump with fluorescence detection (MTJ-F and LTJ-F, respectively) to monitor the fluorescence changes of Tyr-59 located on the 3(10)-helix. The kinetics occurs exclusively in the millisecond to second time range, and the decays are strictly single exponential. From global analysis of folding and unfolding decays, the k(f) and k(u) values were determined, without use of the equilibrium constant K. The activation enthalpy of folding is negative (Delta H-f(#)(T-m) = -10.8 kcal/mol), but the free energy of the transition state is substantially larger than that of the unfolded state (Delta G(f)(#)(T-m) = 7.6 kcal/mol >> RTm). Thus, wt-UBQ behaves as a two-state folder, when folding is monitored by the fluorescence of Tyr-59. The observation of kinetics on the microsecond time scale, when folding is monitored by the disruption of hydrogen bonds between beta-strands, using nonlinear infrared spectroscopy of the amide I vibrations (LTJ-DVE) [Chung, H. S.; Tokmakoff, A. Proteins: Struct., Funct., Bioinf 2008, 72, 474-487], seems to result from the fact that MTJ-F monitors the effective unfolding (backbone exposure to water) of the thermally excited protein alone, while LTJ-DVE also monitors preliminary events (hydrogen-bond breaking) and thermal re-equilibration of the thermally excited protein.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available