Article
Chemistry, Physical
Thu Thi-Yen Le, Siam Hussain, Ruey-Yug Tsay, Boris A. Noskov, Alexander Akentiev, Shi-Yow Lin
Summary: This study demonstrates a more precise and accurate method for measuring the equilibrium surface tension (ST) of bovine serum albumin (BSA) in aqueous solutions. Regardless of the BSA concentration, the ST remains constant at the later stage. These results are further confirmed by a perturbation process. The findings indicate that the equilibrium ST of BSA solutions remains essentially unchanged over a wide range of BSA concentration.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Biochemistry & Molecular Biology
Blaz Zdovc, Matej Jaklin, Barbara Hribar-Lee, Miha Luksic
Summary: The pharmaceutical design of protein formulations aims at maximizing efficiency by increasing protein concentration while minimizing viscosity. The study found that the viscosity of BSA in an aqueous 20 mM acetate buffer increases with protein concentration but decreases with higher temperatures, with the viscosity decreasing by almost 60% between 5 and 45 degrees Celsius. While the modified Arrhenius theory provided a quantitative agreement with the experimental results, a hard-sphere model only offered a qualitative description.
Article
Chemistry, Physical
Juan C. Sanabria, Carmen M. Romero
Summary: The adsorption dynamics of bovine serum albumin in water and aqueous solutions of alkylammonium salts were studied. The results showed that the adsorption kinetics in the solutions can be described by a three-step model, with diffusion-controlled transport of the protein in the initial stage, followed by adsorption and penetration as well as rearrangement at the air-liquid interface. The analysis indicated that the second step is the slowest in the overall adsorption process.
JOURNAL OF MOLECULAR LIQUIDS
(2022)
Article
Chemistry, Physical
Marcia M. S. Alves, Joao M. M. Araujo, Ivo C. Martins, Ana B. Pereiro, Margarida Archer
Summary: The study investigates the interaction of fluorinated ionic liquids with bovine serum albumin (BSA), demonstrating the potential application value of fluorinated ionic liquids in stabilizing and structurally affecting BSA.
JOURNAL OF MOLECULAR LIQUIDS
(2021)
Article
Biochemistry & Molecular Biology
Attila Forgacs, Madalina Ranga, Istvan Fabian, Jozsef Kalmar
Summary: Mesoporous silica aerogels have potential applications in various fields, and understanding their interactions with biomolecules is crucial for optimal performance. This study investigated the interactions between bovine serum albumin (BSA) and silica aerogels, revealing a multi-step binding mechanism and the influence of pH on their interaction strength.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Food Science & Technology
Lei Xu, Hu Hou, Brian Farkas, Kevin M. Keener, Allen L. Garner, Bernard Tao
Summary: This study investigated the effects of high voltage atmospheric cold plasma on bovine serum albumin, leading to structural alterations, protein precipitation, and peptide bond cleavage. The findings suggest that interactions between HVACP-induced reactive species and proteins can introduce significant changes to protein structure and composition in aqueous conditions.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2021)
Article
Materials Science, Multidisciplinary
Amin Imani, Mohammadali Shahsavari, R. K. Singh Raman, Edouard Asselin
Summary: The study investigates the effects of albumins from bovine and chicken egg sources on the biodegradation of commercially pure Mg in a saline environment. It reveals that albumins from different sources have varying influences on the degradation behavior of Mg, emphasizing the importance of considering the source of albumin for in vitro assessment of Mg-based biomaterials.
Article
Biochemistry & Molecular Biology
Md. Anamul Hoque, Mohammad Idrish Ali, Malik Abdul Rub, Marzia Rahman, Shahed Rana, Mohammad Majibur Rahman, Dileep Kumar, Naved Azum, Abdullah M. Asiri, Mohammed Abdullah Khan
Summary: In this study, the association behavior of bovine serum albumin (BSA) and cetyl-trimethylammonium bromide (CTAB) was investigated using the conductivity method. The association phenomenon was detected by changes in conductivity and various physico-chemical properties. The interaction between BSA and CTAB was influenced by temperature and hydration extent. The binding interactions were believed to involve H-bonding, ion-dipole, and hydrophobic interactions.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Chemistry, Multidisciplinary
Mengmeng Zhou, Yutong Bi, Haijun Zhou, Xiaoqi Chen, Fen Zhang, Yantao Li, Xiongwei Qu
Summary: The aggregation behavior between P(AA-co-OMA) and BSA was studied, revealing optimal composite conditions and a reliance on hydrophobic interactions.
Article
Engineering, Chemical
Marcelo da Silva Pedro, Luiz Andre Fialho Oliveira, Carlos Eduardo de Araujo Padilha, Everaldo Silvino dos Santos, Jackson Araujo de Oliveira, Domingos Fabiano de Santana Souza
Summary: The study investigated the effects of flow patterns on the partitioning of biomolecules using microdevices in ATPE. Results showed that the droplet flow pattern exhibited better recovery and partition coefficient for BSA, while for ampicillin, the extraction performance was almost independent of the flow patterns.
SEPARATION AND PURIFICATION TECHNOLOGY
(2021)
Article
Chemistry, Physical
Chuanli Wu, Wenhui Liang, Jingjing Fan, Yuxian Cao, Ping Wu, Chenxin Cai
Summary: Proteins with small size and favorable electron transport properties are suitable for bioelectronic devices and offer a solution for miniaturization. Modulating the electron transport band gaps of proteins is experimentally challenging but significant. This study presents a method for regulating the band gaps of bovine serum albumin (BSA) through its interaction with hemin, demonstrating the potential for tuning protein characteristics for nanoscale bioelectronic devices.
ACTA PHYSICO-CHIMICA SINICA
(2021)
Article
Chemistry, Multidisciplinary
Ana F. C. S. Rufino, Mafalda R. Almeida, Mukesh Sharma, Joao A. P. Coutinho, Mara G. Freire
Summary: With a novel ionic-liquid-based aqueous biphasic system, efficient extraction and separation of bovine serum albumin (BSA) from bovine serum was achieved, with a recovery yield of 85.6% and purity of 61.2% under optimal conditions. The system shows potential as a platform for purifying BSA from serum samples and can be applied to other valuable proteins.
APPLIED SCIENCES-BASEL
(2022)
Article
Chemistry, Physical
Van Nho Tran, Johann Eduardo Maradiaga Rivas, Siam Hussain, Wen-Chi Tseng, Alexander Akentiev, Boris Noskov, Giuseppe Loglio, Shi-Yow Lin
Summary: The dynamic and equilibrium surface tensions of Human Serum Albumin (HSA) and Bovine Serum Albumin (BSA) solutions were compared in this study, along with the dilational modulus of the adsorbed films. The results showed that HSA had a faster relaxation in dynamic surface tension compared to BSA, while the equilibrium surface tension remained constant for both HSA and BSA at different concentrations. The dilational modulus of the films exhibited similar concentration dependency for HSA and BSA.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Chemistry, Physical
Jingyi Zhang, Xianjun Fu, Changling Yan, Gongke Wang
Summary: This study investigated the morphology-dependent interaction between silver nanoparticles (AgNPs) and proteins. The results showed that the particle size, shape, and dispersion of AgNPs significantly influenced the interaction with proteins. Protein coronas were formed on silver nanospheres and nanorods, leading to slightly enlarged outer sizes. In the presence of proteins, silver nanotriangles gradually transformed into nanodisks. These results indicate that the formation of a protein corona and the aggregation behaviors of AgNPs are markedly determined by their inherent morphologies.
Article
Spectroscopy
Md. Zahirul Kabir, Hafsa Tayyab, Cem Erkmen, Sevinc Kurbanoglu, Saharuddin B. Mohamad, Bengi Uslu
Summary: The interactive association between the antifungal drug climbazole (CBZ) and the carrier protein bovine serum albumin (BSA) was investigated using fluorescence, absorption spectroscopy, and in silico techniques. The results confirmed the formation of a complex between CBZ and BSA. Hydrophobic forces, van der Waals forces, and hydrogen bonds were found to play key roles in stabilizing the complex. The binding of CBZ to BSA occurred near Sudlow's sites I and II, with a greater preference for site II.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Thermodynamics
Daniele Sonaglioni, Elpidio Tombari, Simone Capaccioli
Summary: Fast scanning calorimetry is a useful experimental technique that can suppress reorganization processes. This paper proposes a new method for estimating the dynamical thermal lag and provides a novel interpretation of heat flow losses due to the sample. The use of glass to liquid transition is also shown as a new method to reveal static thermal gradients.
THERMOCHIMICA ACTA
(2023)
Article
Polymer Science
Isala Dueramae, Taisei Ishiyama, Ayumi Torigaki, Shinya Nakano, Kaito Sasaki, Rio Kita, Naoki Shinyashiki, Shin Yagihara, Yukiteru Katsumoto, Masaru Yoneyama
Summary: The dielectric relaxation of poly-(N-isopropylacrylamide) (PNiPAM) in 1,4-dioxane as a low-polarity solvent was investigated under various conditions. The study found that the relaxation is composed of two components: rotational motion of side groups at around 100 MHz and micro-Brownian motion of PNiPAM chain at around 1 MHz. These processes can be described by two Cole-Cole equations. The relaxation times of these processes showed temperature dependence, as evidenced by the activation energies obtained from the Arrhenius plot. Additionally, asymmetric relaxation was observed in the MHz region of PNiPAM in different solvents, suggesting a deeper understanding of PNiPAM's molecular dynamics.
Article
Polymer Science
Shin Yagihara, Seiei Watanabe, Yuta Abe, Megumi Asano, Kenta Shimizu, Hironobu Saito, Yuko Maruyama, Rio Kita, Naoki Shinyashiki, Shyamal Kumar Kundu
Summary: This paper presents a universal water structure analysis of several gels with different structures and gelation mechanisms including polymer gels, supramolecular gels composed of surfactant micelles, and cement gels. The results show that the hydrogen bond network (HBN) of water molecules in these gels undergoes changes at a molecular level, with a decrease in fractal dimension as cross-linking increases, leading to the fragmentation of collective water structures. The tau-beta diagrams analysis method proposed in this paper can be widely applied to characterize diverse gel structures and evaluate gelation processes.
Article
Polymer Science
Kenta Shimizu, Fumiya Abe, Yasuhiro Kishi, Rio Kita, Naoki Shinyashiki, Shin Yagihara
Summary: There are diverse gel materials used in various fields and their gelation mechanisms are extremely varied. In the case of hydrogels, it is challenging to understand the complex molecular mechanisms due to the interaction of water molecules through hydrogen bonding as solvents. This study used broadband dielectric spectroscopy (BDS) to elucidate the molecular mechanism of the structural formation of fibrous supramolecular gel by a low molecular weight gelator, N-oleyl lactobionamide/water mixture.
Article
Chemistry, Physical
Federico Caporaletti, Simone Capaccioli, Dimitrios Bessas, Aleksander I. Chumakov, Alessandro Martinelli, Giulio Monaco
Summary: The JG relaxation is closely connected to the structural relaxation in supercooled liquids, and investigating its microscopic properties is crucial for understanding the glass-transition. In this study, we used time-domain interferometry to probe the microscopic density fluctuations of the van der Waals glass-former cumene. Our results show that the molecules involved in the JG relaxation undergo a restricted motion, leading to local cage-breaking events at the characteristic time-scale for molecular re-orientations.
JOURNAL OF MOLECULAR LIQUIDS
(2023)
Article
Biology
Kahori Furuhata, Haruchika Masuda, Atsuko Sato, Kumiko Miyata, Naoki Shinyashiki, Rio Kita, Kotaro Imagawa, Tadashi Akamatsu, Shin Yagihara
Summary: This study examined the in vivo water structure dynamics (WSD) between physiological and pathophysiological statuses. The results showed that tumor-bearing mice exhibited aberrant WSD compared to healthy mice. Dielectric spectroscopy may provide novel pathophysiological perspectives in tumor-bearing living bodies.
Article
Chemistry, Physical
Balachandar Vijayakumar, Masanobu Takatsuka, Kaito Sasaki, Rio Kita, Naoki Shinyashiki, Shin Yagihara, Sampathkumar Rathnasabapathy
Summary: A broadband dielectric spectroscopy study was conducted to investigate the dielectric relaxation of ice in a partially crystallized poly(N-isopropylacrylamide) microgel aqueous suspension. Two relaxation processes were observed, one associated with the local chain motion of the microgel and interfacial polarization, and the other associated with ice. The temperature dependence of the ice relaxation time was found to be independent for concentrations ≥10 wt% and the influence of different polymer water mixtures on ice crystallization was studied.
PHYSICAL CHEMISTRY CHEMICAL PHYSICS
(2023)
Article
Polymer Science
Fatima Ezzahra Bouharras, Massimiliano Labardi, Elpidio Tombari, Simone Capaccioli, Mustapha Raihane, Bruno Ameduri
Summary: The dielectric properties of PVDF-g-BT core-shell structured nanocomposites obtained by RAFT polymerization were investigated. The dielectric constant increased with the addition of BT, exhibiting a larger increase than predicted by usual modeling methods for composite materials, due to the effect of the interfacial core-shell structure. PVDF's known dielectric relaxations were observed in both the neat polymer and its nanocomposites, unaffected by the presence of nanoparticles. A relaxation process due to interfacial polarization was found at higher temperatures, attributed to the high crystallinity of the materials produced by RAFT.