Article
Chemistry, Analytical
Youli Tian, Yiren Cao, Genhan Zha, Ke-Er Chen, Muhammad Idrees Khan, Jicun Ren, Weiwen Liu, Yuxing Wang, Qiang Zhang, Chengxi Cao
Summary: This study proposes a marker-free isoelectric focusing method that uses a convolutional neural network model to efficiently and accurately classify isoelectric focusing patterns. A dataset of 1449 isoelectric focusing patterns was collected and experimental pH gradient drift was introduced to enhance the model's robustness. With simple preprocessing, rapid inference speed (only 50 ms), and a high accuracy of 97.1%, the CNN model outperformed traditional algorithms in simultaneously identifying meat species and cuts, indicating its great potential for large-scale isoelectric focusing analyses of complex protein samples when combined with microstrip isoelectric focusing.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Multidisciplinary
Yera Ussembayev, Filip Beunis, Lucas Oorlynck, Mohammadreza Bahrami, Filip Strubbe, Kristiaan Neyts
Summary: This study observed single binding-unbinding events occurring at the surface of suspended nanoparticles in water and achieved elementary charge resolution. The discovery offers prospective routes for studying the dynamics of various chemical and biological phenomena on the nanoscale.
Article
Medical Laboratory Technology
Junko Fujihara, Yoshikazu Takinami, Yasuyuki Kawai, Kaori Kimura-Kataoka, Haruo Takeshita
Summary: The study found significantly higher serum cfDNA concentrations in postmortem subjects compared to healthy controls and patients with cardiac disease, especially in those who died from asphyxia. Fragment larger than 10,000 bp was rare in postmortem samples, while a high concentration of 150-200 bp fragments was characteristic of postmortem subjects.
CLINICA CHIMICA ACTA
(2021)
Article
Chemistry, Multidisciplinary
Taehoon Sung, Min-Kyu Song, Se-Yeon Jung, Sein Lee, Young-Woong Song, Solah Park, Jang-Yeon Kwon
Summary: This research presents a method to reduce the resistance of amorphous indium-gallium-zinc-oxide (a-IGZO) using a vacuum-free solution-based metallization (VSM) process. By dipping the a-IGZO into trimethyl aluminium (TMA) solution, the conductivity of a-IGZO can be significantly enhanced. Self-aligned top-gate (SATG) a-IGZO thin-film-transistors (TFTs) were successfully fabricated using the VSM process, and the mechanism was explained using X-ray photoelectron spectroscopy (XPS).
Article
Chemistry, Analytical
Steven Doria, Jarad Yost, Zachary Gagnon
Summary: The ability to preconcentrate, separate, and purify biomolecules is important for portable diagnostic tools. A new technique called teichophoresis (TPE) is presented for the concentration and separation of proteins and nucleic acids. TPE does not require a leading electrolyte and allows for continuous biomolecular concentration. It is biocompatible, requires low voltage, and produces no significant pH changes.
Article
Chemistry, Multidisciplinary
Sebastiano Caccamo, Rosaria Anna Puglisi
Summary: This study presents a molecular doping method using phosphoric acid as a dopant precursor, which allows for higher predetermined surface density and electrical carrier doses compared to traditional methods using organic molecules as precursors.
Article
Mathematics, Applied
Georg Schluechtermann, Michael Wibmer
Summary: This paper derives a free analogue of the Euler-Maruyama method (fEMM) for numerically approximating solutions of free stochastic differential equations (fSDEs). By applying the theory of multiple operator integrals, a free Ito <^>\ formula is derived and iterated to define fEMM. Strong and weak convergence in the fSDE setting are considered and convergence orders are proven. Numerical examples support the theoretical results and showcase solutions where no analytical solution is available.
SIAM JOURNAL ON NUMERICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Tianyu Yan, Huaizhong Zhang, Kristen A. Fichthorn
Summary: In this study, two variants of replica-exchange molecular dynamics (MD) simulations were used to investigate the minimum free-energy shapes of Ag nanocrystals containing 100-200 atoms. A shape intermediate between Dh and Ih, called Dh-Ih, was discovered with distinct structural signatures and magic sizes. The shapes of the nanocrystals were found to be influenced by the chemical identities of the solution-phase molecules, and changes in shape were observed with the addition or removal of single atoms or at certain critical sizes and temperatures.
Article
Biochemical Research Methods
Lindsay Schneider, Anubhav Tripathi
Summary: This research presents a new method for identifying and separating target DNA of the same size based on targeted sequences. The method allows for the evaluation of multiple target analytes in a sample without fluorescence detection. The method utilizes microfluidic electrophoresis to separate different 150 bp target sequences into different sizes. It has the potential to be used for mutation detection and multiplexed analysis.
ANALYTICAL BIOCHEMISTRY
(2022)
Article
Multidisciplinary Sciences
Anne-Lise Marie, Somak Ray, Alexander R. Ivanov
Summary: Alterations of protein glycosylation can be used as sensitive and specific disease biomarkers. The authors developed a label-free workflow using high sensitivity capillary zone electrophoresis-mass spectrometry (CZE-MS) for analyzing native underivatized N-glycans. This method provides a significant increase in signal intensity and allows for highly sensitive analysis of minute sample amounts.
NATURE COMMUNICATIONS
(2023)
Article
Biochemical Research Methods
P. Layton Ashmore, Florentino Valdez, James F. Harbertson, Roger B. Boulton, Thomas S. Collins
Summary: A novel method using CE-UV/vis was developed for the determination of free SO2 in wine and cider. The CE method showed significant differences with conventional methods, especially in the presence of anthocyanins. The CE method was found to be rapid, sensitive, robust, and repeatable, overcoming the issue of over-reporting free SO2 in pigmented samples.
JOURNAL OF CHROMATOGRAPHY A
(2023)
Article
Astronomy & Astrophysics
Giovanni Montani, Roberta Chiovoloni
Summary: Through the WKB characterization and the dynamics of the inhomogeneous mixmaster, this study explores the emergence of nonsingular generic cosmological solutions in the Universe, suggesting the possibility of acquiring nonsingular classical behavior while retaining microscopic quantum degrees of freedom.
Article
Polymer Science
Yi-Ju Chen, Hsiu-Yu Yu
Summary: We conducted molecular dynamics simulations to investigate the impact of enthalpic interaction on the static and dynamic properties of solvent-free polymer brushes. The results showed that a less favorable enthalpic interaction led to a more stretched chain configuration, lower inter-brush penetration, and faster relaxation. Additionally, the molecular absorption was found to be driven by the release of entropic frustration and the chemical affinity. The distribution function of solute within the inter-wall space indicated that solute-polymer mixing occurred preferentially in the middle of the gap when the enthalpic interaction was more favorable. The brush profiles restrained the molecular diffusion perpendicular to the grafting wall, with higher solute mobility observed when the attraction from the brush was weaker.
Article
Chemistry, Analytical
Nobuyuki Futai, Yuto Fukazawa, Tomohiro Kashiwagi, Shogo Tamaki, Riho Sakai, Catherine A. Hogan, Kanagavel Murugesan, Ashwin Ramachandran, Niaz Banaei, Juan G. Santiago
Summary: This study presents a method for the high-efficiency separation and extraction of short cfDNA fragments, using a millimeter-scale fluidic device to extract the fragments into solid agarose gel slabs from liquid human plasma samples. The method demonstrates high sensitivity in cfDNA detection.
ANALYTICA CHIMICA ACTA
(2022)
Article
Mathematics, Applied
Zhaohua Gong, Chongyang Liu, Kok Lay Teo, Song Wang, Yonghong Wu
Summary: The main purpose of this study is to develop a numerical solution method for solving a class of nonlinear free final time fractional optimal control problems, and the effectiveness of the developed algorithm is demonstrated through numerical simulations.
APPLIED MATHEMATICS AND COMPUTATION
(2021)
