Article
Microbiology
June Kim, Changwon Kim, Siin Kim, Hyotcherl Ihee, Woonsup Shin, Eui-Jin Kim, Jeong K. Lee
Summary: Rhodobacter sphaeroides mutant BF lacks BchF and accumulates Chlide a and 3V-Bchlide a. This mutant grows photoheterotrophically, implying a functional V-RC, which is confirmed by the emergence of growth-competent suppressors of BC under irradiation. The V-RC has similar electron transfer properties to the WT-RC but has slower electron transfer and a higher midpoint redox potential.
MICROBIOLOGY SPECTRUM
(2023)
Article
Biochemistry & Molecular Biology
Nikki Cecil M. Magdaong, James C. Buhrmaster, Kaitlyn M. Faries, Haijun Liu, Gregory A. Tira, Jonathan S. Lindsey, Deborah K. Hanson, Dewey Holten, Philip D. Laible, Christine Kirmaier
Summary: The study describes the substitution of all possible natural amino acids for LeuL185 near the B-side bacteriopheophytin in bacterial reaction center. Different mutations result in the presence of magnesium chlorin or bacteriochlorophyll a in the place of H-B, leading to the formation of a charge-separated state P+CB- in some cases. This finding suggests new strategies for amino acid control over pigment type at specific sites in photosynthetic proteins.
Article
Chemistry, Physical
Vytautas Bubilaitis, Olga Rancova, Darius Abramavicius
Summary: Exciton energy relaxation in a bacterial Reaction Center (bRC) pigment-protein aggregate may involve emission of high energy vibrational quanta. The study assessed this hypothesis using vibronic two-particle theory and found that specific high frequency vibrations may be responsible for the excitation relaxation process.
JOURNAL OF CHEMICAL PHYSICS
(2021)
Article
Multidisciplinary Sciences
Daniel Jun, Sylvester Zhang, Adrian Jan Grzedowski, Amita Mahey, J. Thomas Beatty, Dan Bizzotto
Summary: The use of biomacromolecules in clean alternative energies is a developing field, with potential applications in biosensors and biophotovoltaic devices. Research has shown that the assembly of bacterial photosynthetic reaction center (RC) on electrodes and its local environment significantly impact photocurrent generation, with implications for electron transfer of other biomacromolecules in sensor and photovoltaic applications.
Article
Chemistry, Physical
Owen Thwaites, Bern M. M. Christianson, Alexander J. J. Cowan, Frank Jackel, Lu-Ning Liu, Adrian M. M. Gardner
Summary: Elucidating the photosynthetic processes within RC-LH1 supercomplexes is crucial for understanding natural photosynthetic systems and developing artificial photosynthesis. This study examined the energy transfer in RC-LH1 supercomplexes of Rhodobacter sphaeroides and investigated the roles of transmembrane polypeptides PufX and PufY. The results showed that the absence of PufX increased the excitation energy transfer lifetime and distribution, while the absence of PufY led to conformational changes in LH1 subunits but did not affect the energy transfer lifetime significantly.
JOURNAL OF PHYSICAL CHEMISTRY B
(2023)
Article
Microbiology
Haoyu Wang, Xiaoling Sha, Rui Li, Yijing Li, Himel Nahreen Khaleque, Yuxiu Zhang, Tsing Bohu, Zhihui Bai, Xuliang Zhuang
Summary: This study conducted a genome-wide comparative analysis of the strain EBL0706, confirming it as a strain of Luteovulum azotoformans. The genome of EBL0706 contains 4034 protein-coding genes, including a complete photosynthetic gene cluster.
Article
Fisheries
Chewapat Saejung, Anuwat Chaiyarat, La-orsri Sanoamuang
Summary: The use of Rhodopseudomonas faecalis PA2 as a feed for fairy shrimp has been shown to enhance their growth and survival rates. Additionally, comparing different forms of the bacterial powder revealed that air-dried and freeze-dried R. faecalis PA2 are beneficial for fairy shrimp and do not affect water quality.
Article
Multidisciplinary Sciences
Mariann Kis, James L. Smart, Peter Maroti
Summary: Light-induced oxidation of the reaction center dimer and periplasmic cytochromes was studied in two anaerobic phototrophic bacteria. The research revealed the important roles of light intensity, electron transfer rate, and reduced cytochrome concentration in the oxidation process.
SCIENTIFIC REPORTS
(2022)
Article
Microbiology
Shuhei Hayashi, Yasunari Iwamoto, Yuki Hirakawa, Koichi Mori, Naoki Yamada, Takaaki Maki, Shinjiro Yamamoto, Hitoshi Miyasaka
Summary: The research on the supernatant and pellet of Rhodobacter sphaeroides showed that both fractions have growth-promoting effects on Brassica rapa var. perviridis, with the supernatant requiring high concentrations and the pellet having growth-promoting effects at low concentrations. Additionally, lipopolysaccharide (LPS) was identified as one of the active principles responsible for the plant-growth-promoting effect of PNSB, with foliar feeding of R. sphaeroides LPS significantly enhancing plant growth.
Article
Chemistry, Physical
Giordano Amoruso, Juntai Liu, Daniel W. Polak, Kavita Tiwari, Michael R. Jones, Thomas A. A. Oliver
Summary: RCs are crucial components of natural photosystems, converting solar energy into a potential difference that powers much of biology. By coupling RCs with synthetic light-harvesting materials, such as a nanoconjugate assembled from a synthetic quantum dot (QD) antenna and a tailored fluorescent RC, the efficiency of energy transfer from QDs to attached RCs can be achieved within a short timescale of 26.6 ns with high efficiency of 0.75.
JOURNAL OF PHYSICAL CHEMISTRY LETTERS
(2021)
Article
Biochemical Research Methods
Gabriella Buscemi, Massimo Trotta, Danilo Vona, Gianluca M. Farinola, Francesco Milano, Roberta Ragni
Summary: A supramolecular construct for solar energy conversion is developed by chemically bridging the reaction center (RC) from Rhodobacter sphaeroides and cytochrome c (Cyt c) proteins with a tailored organic light harvesting antenna (hCy2). The RC-hCy2-Cyt c biohybrid mimics the working mechanism of biological assemblies in bacterial cell membrane to convert sunlight into metabolic energy. The biohybrid shows significantly enhanced photoactivity and photocurrent compared to the pristine RC and equimolar mixture of unbound proteins, respectively. These results provide interesting insights into photoenzyme chemical manipulation and pave the way for new eco-sustainable systems in biophotovoltaics.
BIOCONJUGATE CHEMISTRY
(2023)
Article
Physics, Applied
E. Alfinito, R. Cataldo, L. Reggiani
Summary: Biological matter is gaining attention for its innovative features and applications in technology. This study explores the use of a photosensitive protein to build an electrical pH sensor, demonstrating the feasibility of the bio-rheostat hypothesis and supporting the development of electron devices based on biomaterials.
APPLIED PHYSICS LETTERS
(2022)
Article
Chemistry, Physical
K. Dubas, S. Szewczyk, R. Bialek, G. Burdzinski, M. R. Jones, K. Gibasiewicz
Summary: The study found that light-induced electron transfer reactions in mutant Rhodobacter sphaeroides reaction centers can result in acceleration or deceleration compared to wild-type protein. Analysis showed electron resonance between P-BA (+) and P+HA- states, forming an intermediate charged-separated state during charge recombination.
JOURNAL OF PHYSICAL CHEMISTRY B
(2021)
Article
Multidisciplinary Sciences
Laura Bracun, Atsushi Yamagata, Bern M. Christianson, Tohru Terada, Daniel P. Canniffe, Mikako Shirouzu, Lu-Ning Liu
Summary: The RC-LH1-PufX supercomplex has a unique structure with PufX acting as a molecular cross brace to reinforce the RC-LH1 structure, providing strong photosynthetic function and electron transfer.
Article
Biochemistry & Molecular Biology
I. Satoh, K. Gotou, S. Nagatsuma, K. V. P. Nagashima, M. Kobayashi, L. -j. Yu, M. T. Madigan, Y. Kimura, Z. -y. Wang-Otomo
Summary: Phospholipid-protein interactions are important for photosynthetic membranes. This study characterizes the phospholipid composition of intracytoplasmic membranes (ICM) from genetically altered Rhodobacter sphaeroides strains and finds selective interactions between the complexes and specific phospholipids. Expression of different light-harvesting (LH) complexes alters the phospholipid composition, and purified LH1-reaction center core complexes have different phospholipid compositions. The study also investigates the effects of carotenoids on phospholipid composition.
BIOCHIMICA ET BIOPHYSICA ACTA-BIOENERGETICS
(2023)
