Journal
JOURNAL OF PHYCOLOGY
Volume 46, Issue 6, Pages 1218-1228Publisher
WILEY
DOI: 10.1111/j.1529-8817.2010.00911.x
Keywords
copper; Cu; diatom; Fe; iron; kinetics; Thalassiosira; transport
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Funding
- Natural Sciences and Engineering Research Council of Canada (NSERC)
- TRIUMF
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We investigated copper (Cu) acquisition mechanisms and uptake kinetics of the marine diatoms Thalassiosira oceanica Hasle, an oceanic strain, and Thalassiosira pseudonana Hasle et Heimdal, a coastal strain, grown under replete and limiting iron (Fe) and Cu availabilities. The Cu-uptake kinetics of these two diatoms followed classical Michaelis-Menten kinetics. Biphasic uptake kinetics as a function of Cu concentration were observed, suggesting the presence of both high- and low-affinity Cu-transport systems. The half-saturation constants (K-m) and the maximum Cu-uptake rates (V-max) of the high-affinity Cu-transport systems (similar to 7-350 nM and 1.5-17 zmol center dot mu m-2 center dot h-1, respectively) were significantly lower than those of the low-affinity systems (> 800 nM and 30-250 zmol center dot mu m-2 center dot h-1, respectively). The two Cu-transport systems were controlled differently by low Fe and/or Cu. The high-affinity Cu-transport system of both diatoms was down-regulated under Fe limitation. Under optimal-Fe and low-Cu growth conditions, the K-m of the high-affinity transport system of T. oceanica was lower (7.3 nM) than that of T. pseudonana (373 nM), indicating that T. oceanica had a better ability to acquire Cu at subsaturating concentrations. When Fe was sufficient, the low-affinity Cu-transport system of T. oceanica saturated at 2,000 nM Cu, while that of T. pseudonana did not saturate, indicating different Cu-transport regulation by these two diatoms. Using CuEDTA as a model organic complex, our results also suggest that diatoms might be able to access Cu bound within organic Cu complexes.
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