Journal
JOURNAL OF PHYCOLOGY
Volume 45, Issue 2, Pages 517-521Publisher
WILEY-BLACKWELL PUBLISHING, INC
DOI: 10.1111/j.1529-8817.2009.00651.x
Keywords
cyanobacteria; DNA; fluorometry; humics; lake water; microcystin; PEG; PVPP; sediment; xanthogenate
Categories
Funding
- St. Johns River Water Management District
Ask authors/readers for more resources
DNA isolated from environmental samples often contains enzyme inhibitors disruptive to downstream molecular applications. Most of the existing methods of cyanobacterial DNA isolation do not effectively eliminate these inhibitors from sediment samples or cells collected from freshwater ecosystems. We describe improved methods based on the xanthogenate-SDS nucleic acid isolation (XS) method of Tillett and Neilan (2000). Our improved methods provided high-quality cyanobacterial DNA that could be amplified in PCR and digested with a restriction enzyme. Results were superior to several commercial kits. The DNA yield was also similar to that obtained via the standard XS method. These methods should provide valuable new tools for the expanded application of molecular genetics to limnological and oceanographic research.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available