Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
Volume 104, Issue 3, Pages 405-413Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotobiol.2011.04.008
Keywords
Chlorophenols; Lysozyme; Fluorescence; Interaction
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Funding
- Fund for Excellent Innovation Team of Jiangsu Provincial Department of Education [2009-23]
- National Natural Science Foundation of China [30570218]
- Natural Science Foundation of Education Department of Jiangsu Province [07KJA18017]
- Educational Bureau [09KJD150007]
- Scientific Foundation of Yancheng Teachers University [333]
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The binding interactions of lysozyme with 2-chlorophenol, 2,4-dichlorophenol, 2,4,6-trichlorophenol and pentachlorophenol were investigated by UV-vis absorption, CD, fluorescence, synchronous fluorescence, and three-dimensional fluorescence spectra techniques under physiological pH 7.40. The binding constants, quenching mechanism, and the number of binding sites were determined by the quenching of lysozyme fluorescence in presence of chlorophenols. H-bonds and hydrophobic interactions played major roles in stabilizing the chlorophenols-lysozyme complex. The distances r between chlorophenols and lysozyme were calculated to be 1.94 nm, 2.75 nm, 3.54 nm, and 3.76 nm for 2-CP, 2,4-DCP, 2,4,6-TCP, and PCP, respectively. The effects of chlorophenols on the conformation of lysozyme were analyzed using CD, synchronous fluorescence and three-dimensional fluorescence spectra. (C) 2011 Elsevier B.V. All rights reserved.
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