Journal
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY A-CHEMISTRY
Volume 277, Issue -, Pages 75-81Publisher
ELSEVIER SCIENCE SA
DOI: 10.1016/j.jphotochem.2013.12.012
Keywords
Molecular probe; Fluorescence bioimaging; Gram seed sprouts; Intracellular detection; Zinc translocation
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Funding
- Department of Science and Technology, India [SERB/F/2408/2012-13]
- IIT Mandi
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Three TRIS-based molecular probes, L1/L2/L3, were screened for their ability to detect Zn2+ at nanomolar level. Keeping in mind the criteria for a molecular probe to be utilized as bioimaging material, L2 was chosen as model compound for biological studies due to its higher excitation/emission wavelengths. While investigating the potential of L2, intracellular Zn2+ was successfully imaged using fluorescence microscopy. As the fluorescence emission of L2-Zn2+ ensemble was stable for longer duration, more than 3 h, L2 was successfully utilized in in vivo imaging of Zn2+ in a whole plant (Peperomia pellucida) in real-time. Further to its in vivo imaging capability, 12 was found to be successful in imaging zinc pool of gram seed sprouts. (C) 2013 Elsevier B.V. All rights reserved.
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