Journal
JOURNAL OF PHARMACY AND PHARMACOLOGY
Volume 61, Issue 10, Pages 1375-1382Publisher
WILEY
DOI: 10.1211/jpp/61.10.0015
Keywords
antioxidation; D-galactosamine; hypoxia-inducible factor-1 alpha; lipopolysaccharide; nitric oxide; salidroside
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Funding
- National Natural Science Foundation of China [30660225, 30711140382]
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Objectives The aim was to investigate the protective effect of salidroside isolated from Rhodiola sachalinensis A. Bor. (Crassulaceae) on D-galactosamine/lipopolysaccharide-induced fulminant hepatic failure. Methods Hepatotoxicity was induced by an intraperitoneal injection Of D-galactosamine (700 mg/kg) and lipopolysaccharide (10 mu g/kg); salidroside (20, 50 and 400 mg/kg) was administered intraperitoneally I h before induction of hepatoxicity. Liver injury was assessed biochemically and histologically. Key findings Salidroside attenuated the induced acute increase in serum aspartate aminotransferase and alanine aminotransferase activities, and levels of tumour necrosis factor-alpha levels and serum nitric oxide. It restored depleted hepatic glutathione, superoxide dismutase, catalase and glutathione peroxidase activities, decreased malondialdehyde levels and considerably reduced histopathological changes. Histopathological, immunohistochemical and Western blot analyses also demonstrated that salidroside could reduce the appearance of necrotic regions and expression of caspase-3 and hypoxia-inducible factor-1 alpha in liver tissue. Conclusions Salidroside protected liver tissue from the oxidative stress elicited by D-galactosamine and lipopolysaccharide. The hepatoprotective mechanism of salidroside appear to be related to antioxidant activity and inhibition of hypoxia-inducible factor-1 alpha.
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