Article
Polymer Science
Ali Dehshahri, Bahman Khalvati, Zahra Taheri, Farshad Safari, Reza Mohammadinejad, Abolfazl Heydari
Summary: This study evaluated the transfection efficiency and cytotoxicity of N-[(2-hydroxy-3-trimethylammonium)propyl] chitosan salt (HTCS) as a gene carrier. HTCS was synthesized and used for the delivery of the interleukin-12 (IL-12) gene plasmid. The results showed that HTCS could effectively condense the IL-12 plasmid into polyplexes and demonstrated higher hIL-12 production compared to CS and polyethylenimine polyplexes in cell transfection experiments.
Article
Pharmacology & Pharmacy
Spela Kos, Masa Bosnjak, Tanja Jesenko, Bostjan Markelc, Urska Kamensek, Katarina Znidar, Urska Matkovic, Andrej Rencelj, Gregor Sersa, Rosana Hudej, Aneja Tuljak, Matjaz Peterka, Maja Cemazar
Summary: IL-12 is a key cytokine for antitumor activity, and localized delivery systems like gene electrotransfer have shown positive results in clinical trials in the USA. To meet EU regulatory requirements, a clinical grade antibiotic resistance gene-free plasmid encoding IL-12 has been developed and tested in vitro for biological activity, expression level, and plasmid copy number, setting the stage for further in vivo testing and potential human clinical trials.
Article
Multidisciplinary Sciences
Urska Kamensek, Andrej Rencelj, Tanja Jesenko, Tinkara Remic, Gregor Sersa, Maja Cemazar
Summary: This study aimed to evaluate the maintenance of IL-12 plasmids without antibiotic resistance gene in bacterial culture and their transfection efficiency after gene electrotransfer. The results demonstrated that these plasmids can be stably maintained in bacteria and provide sufficient IL-12 expression, showing potential for further in vivo evaluation studies.
Article
Immunology
Katarzyna Wegierek-Ciura, Jagoda Mierzejewska, Agnieszka Szczygiel, Joanna Rossowska, Anna Wroblewska, Marta Switalska, Tomasz M. Goszczynski, Bozena Szermer-Olearnik, Elzbieta Pajtasz-Piasecka
Summary: This study aimed to determine whether administering HES-MTX before immunotherapy with anti-IL-10R antibodies would change the effectiveness of dendritic cell vaccines that overproduce proinflammatory cytokines IL-12, IL-15, or IL-18. The results showed that using the HES-MTX nanoconjugate created optimal conditions for the effective action of these vaccines in a murine colon carcinoma model. The treatment schedule may be promising in anticancer therapy.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Zhendong Ding, Mengmiao Mo, Kai Zhang, Yongguang Bi, Fansheng Kong
Summary: In this study, PC-CS-NPs were successfully prepared and demonstrated sustainable release of proanthocyanidins, excellent scavenging ability of free radicals, and inhibitory effects on bacterial growth. The findings suggest that the use of chitosan nanoparticles can enhance the bioactivity of proanthocyanidins.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Article
Pharmacology & Pharmacy
Fatemeh Soltani, Hossein Kamali, Abbas Akhgari, Mahboobeh Ghasemzadeh Rahbardar, Hadi Afrasiabi Garekani, Ali Nokhodchi, Fatemeh Sadeghi
Summary: In this study, chitosan nanoparticles loaded with budesonide were prepared using the ionic gelation technique to improve the delivery of orally administered nanoparticles to the colon. The size, zeta potential, encapsulation efficiency, shape, and drug release of the nanoparticles were investigated. The nanoparticles were then pelletized using the extrusion-spheronization method and coated with enteric and time-dependent polymers for colon-specific delivery. The formulations were tested for their anti-inflammatory effect in rats with induced colitis, and the relapse of colitis after treatment discontinuation was also monitored. The results showed that the pelletized budesonide nanoparticles released the drug steadily throughout the gastrointestinal tract, leading to long-lasting anti-inflammatory effects.
Article
Polymer Science
Renato Nunes, Ana Sofia Serra, Aiva Simaite, Angela Sousa
Summary: Nucleic acid vaccines are a fast, safe, cost-effective, and efficient technology for combating viral infections. Developing methods to protect, carry, and deliver nucleic acids is crucial for ensuring their effectiveness.
Article
Oncology
Yosuke Sato, Yu Fu, Hong Liu, Min Young Lee, Michael H. Shaw
Summary: By analyzing the tumor immune characteristics of two murine colorectal cancer models, CT-26 and Colon 26, we identified their different sensitivity to anti-PD-1 therapy, with CT-26 showing more infiltrated immune cells. Genomic/transcriptomic analyses revealed differences in the Wnt pathway activity between the two models, suggesting a potential link to anti-PD-1 response.
Article
Chemistry, Multidisciplinary
Rahaf M. Ahmad, Yaser E. Greish, Hesham F. El-Maghraby, Loay Lubbad, Yahia Makableh, Fayez T. Hammad
Summary: Chitosan-alginate nanoparticles loaded with Nerolidol were successfully fabricated and characterized, showing potential encapsulation and delivery using an in vitro model. Various techniques including Fourier transform infrared spectroscopy, scanning and transmission electron microscopy were used for nanoparticle characterization.
Article
Pharmacology & Pharmacy
Yan Mu, Xiao-He Ren, Di Han, Ying-Ying Guan, Pei-Ling Liu, Si-Xue Cheng, Hong Liu
Summary: The combined delivery of siRNA and pIL-12 effectively inhibits hepatitis B virus, reverses virus-induced immunosuppression, activates antiviral immunity, and hinders the progression of HBV-induced hepatocellular carcinoma.
Article
Biochemistry & Molecular Biology
Renas Rzgar Jalal, Twana Mohammed M. Ways, Mahmoud H. Abu Elella, Diyar Ahmed Hassan, Vitaliy V. Khutoryanskiy
Summary: Mucoadhesive nanoparticles based on methacrylated chitosan (MeCHI) were designed and compared with unmodified chitosan nanoparticles for the delivery of ciprofloxacin. The MeCHI nanoparticles showed smaller particle size, lower polydispersity index, higher encapsulation efficiency, and sustained drug release compared to their chitosan counterpart. The mucoadhesion study also demonstrated that the ciprofloxacin-loaded MeCHI nanoparticles had better retention on the mucosal surface.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2023)
Article
Biochemistry & Molecular Biology
Ezgi Kizilkonca, Emrah Torlak, F. Bedia Erim
Summary: The effects of cerium oxide nanoparticles on chitosan-based films were investigated, showing enhanced antibacterial activity. These composite films, based on chitosan, exhibit flexibility, antibacterial efficacy, and good mechanical strength, making them suitable for packaging or coating materials.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2021)
Review
Chemistry, Physical
Anton R. Egorov, Anatoly A. Kirichuk, Vasili V. Rubanik, Vasili V. Rubanik Jr, Alexander G. Tskhovrebov, Andreii S. Kritchenkov
Summary: This comprehensive review explores the methods of chitosan extraction, its antibacterial properties, and its applications in various sectors. It investigates the relationship between chitosan's properties and antibacterial activity, and highlights the potential of chitosan-based materials. However, the challenges of optimizing chitosan production for sustainability and efficiency remain to be addressed.
Article
Pharmacology & Pharmacy
Shafi Ullah, Asif Nawaz, Arshad Farid, Muhammad Shahid Latif, Muhammad Fareed, Shakira Ghazanfar, Charis M. Galanakis, Abdulhakeem S. Alamri, Majid Alhomrani, Syed Mohammed Basheeruddin Asdaq
Summary: The study aimed to embed folate-modified chitosan 5FU nanoparticles into calcium alginate beads for colon-targeted delivery. The results showed that the swelling, erosion, and water uptake properties of FA-CS-5FU-NP-Bf beads were significantly higher than those of FA-CS-5FU-NP-Bo beads at simulated intestinal pH.
Article
Materials Science, Multidisciplinary
Bin Wang, Haomin Wang, Zhi Wang, Jie Tang, Xianting Yuan, Ying Zhang, Hongjun Chen, Wenjie Yu, Meng Song
Summary: Disease transmission and cross-infection caused by drug-resistant bacteria are serious threats to public health. Therefore, the development of new antibacterial materials that do not induce drug resistance is of great importance. In this study, an organic/inorganic antibacterial composite with a contact/release mechanism was successfully synthesized, showing good bactericidal effects and low cytotoxicity.
MATERIALS TODAY COMMUNICATIONS
(2023)
Review
Medicine, Research & Experimental
Shaho Ghahremani Dehbokri, Nazila Alizadeh, Alireza Isazadeh, Amir Baghbanzadeh, Soheil Abbaspour-Ravasjani, Khalil Hajiasgharzadeh, Behzad Baradaran
Summary: Breast cancer is a prevalent and deadly disease, and its incidence rate is increasing globally. Conventional treatment approaches, such as surgery, radiation therapy, and chemotherapy, often yield unsatisfactory outcomes. Therefore, developing innovative strategies, such as immunotherapy, is crucial for effective breast cancer therapy. The blockade of immune checkpoints, particularly cytotoxic T lymphocyte-associated antigen-4 (CTLA-4), has shown promising results in cancer immunotherapy. The use of anti-CTLA-4 antibodies alone or in combination with other therapeutic agents enhances the tumor-suppressive effects of the immune system and improves cancer prognosis. Immune checkpoint inhibitors hold great potential for breast cancer treatment. This review discusses the role of CTLA-4 and its therapeutic potential in breast cancer therapeutics.
CURRENT MOLECULAR MEDICINE
(2023)
Article
Pharmacology & Pharmacy
Monireh Khordadmehr, Hamed Ezzati, Amirali Shahbazfar, Farinaz Jigari-Asl, Behzad Baradaran, Elham Baghbani, Saeed Noorolyai
Summary: The overexpression of miR-451a can enhance the chemosensitivity of breast cancer cells to carboplatin therapy, which may provide a beneficial strategy for future cancer therapy.
PHARMACEUTICAL SCIENCES
(2023)
Review
Oncology
Samane Abbasi, Milad Asghari Totmaj, Masoumeh Abbasi, Saba Hajazimian, Pouya Goleij, Javad Behroozi, Behrouz Shademan, Alireza Isazadeh, Behzad Baradaran
Summary: Over the last decade, novel therapeutic approaches like CAR-T-cell immunotherapy have transformed the treatment perspective of human malignancies. However, this therapy is associated with side effects and faces challenges in manufacturing, engineering, applications, and toxicities. Further studies are needed to enhance efficacy and minimize adverse events.
Article
Oncology
Farid Ghorbaninezhad, Hajar Alemohammad, Basira Najafzadeh, Javad Masoumi, Mahdi Abdoli Shadbad, Mohammad Shahpouri, Hossein Saeedi, Omid Rahbarfarzam, Behzad Baradaran
Summary: Dexosomes released by dendritic cells can stimulate CD8+ and CD4+ T cell responses, leading to effective anti-tumoral immune responses. Combining dexosomes vaccination with other treatment approaches can significantly increase tumor-specific T cell responses.
Article
Oncology
Elaheh Mohammadali, Reza Safaralizadeh, Arash Poursheikhani, Tooraj Asvadi, Behzad Baradaran, Shahram Teimourian
Summary: This study aimed to evaluate the expression of lncRNA TMPO-AS1 in Iranian patients with gastric cancer (GC). Results showed that TMPO-AS1 was overexpressed in cancerous tissues and could serve as a biomarker to distinguish tumor tissues from normal ones. However, further research is needed to confirm its potential application in the diagnosis, prognosis, and treatment of GC.
INTERNATIONAL JOURNAL OF CANCER MANAGEMENT
(2023)
Article
Oncology
Seyed Samad Hosseini, Paria Nazifi, Mohammad Amini, Felor Zargari, Amir Hossein Yari, Behzad Baradaran, Soltanali Mahboob, Ahad Mokhtarzadeh
Summary: The study showed that crocin decreases cell viability in colorectal cancer cells by upregulating miR-143/145 expression and decreasing KRAS and RREB1 expression dose-dependently. Furthermore, crocin treatment also led to the inactivation of AKT by decreasing its phosphorylation, suggesting a potential inhibitory effect on CRC cell proliferation.
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2023)
Article
Oncology
Behnaz Mansoori, Shiva Kiani, Alireza Ashrafi Mezajin, Pouyan Zandi, Homadokht Banaie, Davoud Rostamzadeh, William C. Cho, Pascal H. G. Duijf, Behzad Mansoori, Behzad Baradaran
Summary: MiR-143-5p expression is lower in breast cancer tissues compared to normal tissues and low expression is associated with poor prognosis in ER-positive breast cancer patients. Restoration of miR-143-5p suppresses metastasis, reduces cell proliferation, stemness, and promotes apoptosis in ER-positive breast cancer cells through direct targeting of HMGA2. Restoring miR-143-5p could be a promising therapeutic approach for ER-positive breast cancer.
CLINICAL BREAST CANCER
(2023)
Review
Multidisciplinary Sciences
Omid Rahbar Farzam, Niloofar Mehran, Farzaneh Bilan, Ehsan Aghajani, Reza Dabbaghipour, Ghazale Asemani Shahgoli, Behzad Baradaran
Summary: Colorectal cancer, a common malignancy with high mortality, can be effectively treated using imaging-guided photothermal therapy combined with nanotechnology. This approach minimizes side effects and improves treatment outcomes, making it significant for colorectal cancer treatment.
Article
Rheumatology
Roghayeh Tofigh, Mohammadali Hosseinpourfeizi, Reza Safaralizadeh, Sepideh Ghoddusifar, Behzad Baradaran
Summary: This study identifies NEAT1, GAS5, and GAPLINC as promising serological biomarkers in rheumatoid arthritis (RA). The reduced expression of NEAT1 and GAS5 suggests their potential roles as diagnostic and prognostic markers, while the significantly elevated average expression of GAPLINC in RA patients' serum samples indicates its potential as a biomarker candidate for disease stratification. TMEVPG1, on the other hand, displayed unaltered expression levels in RA samples.
CURRENT RHEUMATOLOGY REVIEWS
(2023)
Article
Biology
Rozhin Dabbaghi, Reza Safaralizadeh, Shima Rahmani, Nesa Barpour, Mohammadali Hosseinpourfeizi, Ali Rajabi, Behzad Baradaran
Summary: This study investigates the role of T-bet, IFN-gamma, and MEG3 in the pathogenesis of multiple sclerosis (MS) and their potential as diagnostic and therapeutic targets. The results show that T-bet expression is elevated in treatment-naive relapsing-remitting MS (RRMS) patients compared to healthy individuals. RRMS patients treated with different disease modifying therapies (DMTs) exhibit downregulated T-bet and MEG3 expression levels, with more pronounced effects in females. The correlation between T-bet and MEG3 and between IFN-gamma and T-bet is observed in healthy individuals, while a strong positive correlation is found between MEG3 and IFN-gamma in RRMS patients treated with Glatiramer Acetate (GA). Remarkably, RRMS patients treated with Dimethyl Fumarate (DMF) show a significant positive correlation between T-bet and MEG3. These findings suggest the diagnostic potential of T-bet in RRMS and indicate the need for further exploration of the interplay between MEG3, T-bet, and IFN-gamma in RRMS patients.
BMC RESEARCH NOTES
(2023)
Review
Materials Science, Biomaterials
Zahra Ghahramanipour, Shiva Alipour, Javad Masoumi, Arman Rostamlou, Amirhossein Hatami-Sadr, Javad Ahmadian Heris, Bahar Naseri, Mahdi Jafarlou, Behzad Baradaran
Summary: A functional immune system is crucial for protecting us from infections, tumors, or autoimmune disorders. Micronutrients, particularly vitamins, play important roles in maintaining immune system balance. This review focuses on the roles of vitamins (D, E, A, C) and dendritic cells' subsets in immune processes, including functions, maturation, and cytokine production. Current studies show significant benefits of these vitamins, such as the control of dendritic cells' function and maturation by vitamin E, and the immunoregulatory and anti-inflammatory role of vitamin D. The metabolite of vitamin A, retinoic acid, influences T cell differentiation, and vitamin C has antioxidant effects on dendritic cells. The correlation between vitamin levels and allergic diseases or autoimmunity disorders is also discussed based on previous research.
Article
Oncology
Mohammad Heiat, Ehsan Rezaei, Javad Gharechahi, Masoumeh Abbasi, Javad Behroozi, Mohammad Ali Abyazi, Behzad Baradaran
Summary: Silencing SIX4 inhibits the survival, migration, and invasion ability of pancreatic cancer cells, increases apoptosis and autophagy rates, and reduces mitochondrial membrane potential. Furthermore, knockdown of SIX4 affects the expression of genes related to apoptosis and metastasis. Overall, SIX4 plays a critical role in the development of pancreatic cancer.
Review
Oncology
Najibeh Shekari, Dariush Shanehbandi, Tohid Kazemi, Habib Zarredar, Behzad Baradaran, Seyed Amir Jalali
Summary: This article summarizes current evidence on V-domain immunoglobulin suppressor of T cell activation (VISTA), its related ligands, their interactions and effects, as well as preclinical and clinical targeting agents. Investigating VISTA ligands helps understand their functions and roles, and may facilitate the discovery of alternative therapeutic approaches targeting the VISTA pathway.
CANCER CELL INTERNATIONAL
(2023)
Article
Genetics & Heredity
Masoumeh Hajizadeh, Farnaz Hajizadeh, Sevil Ghaffarei, Mohammad Amin Doustvandi, Khadijeh Hajizadeh, Seyyed Mohammad Yaghoubi, Fateme Mohammadnejad, Nadia Allahyarzadeh Khiabani, Pegah Mousavi, Behzad Baradaran
Summary: This review summarizes the role and potential efficacy of miRNAs in promoting and inhibiting cell proliferation and apoptosis in HCC, as well as the role of miRNAs in therapy resistance in HCC.
Article
Medicine, Research & Experimental
Maryam Tohidast, Neda Memari, Mohammad Amini, Seyed Samad Hosseini, Asiyeh Jebelli, Mohammad Amin Doustvandi, Behzad Baradaran, Ahad Mokhtarzadeh
Summary: This study demonstrates that the combination of miR-145 and paclitaxel can inhibit cell proliferation and migration in prostate cancer cells, and enhance chemosensitivity. This combination therapy may be a promising approach for the treatment of prostate cancer.
IRANIAN JOURNAL OF BASIC MEDICAL SCIENCES
(2023)