Review
Physiology
Dora (Jun Ping) Xiong, James G. Martin, Anne-Marie Lauzon
Summary: Asthma is a common chronic inflammatory disease of the airways that varies in its presentation and severity. Airway smooth muscle is the major contributor to airway narrowing and asthma symptoms. Various factors can contribute to the dysfunction of airway smooth muscle. Methodological limitations in studying the airways have hindered our understanding of asthma mechanisms.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Julia Frankenberg Garcia, Andrew Rogers, Judith C. W. Mak, Andrew J. Halayko, Christopher K. M. Hui, Bingling Xu, Kian Fan Chung, Tristan Rodriguez, Charalambos Michaeloudes, Pankaj K. Bhavsar
Summary: Mitochondrial transfer from mesenchymal stem cells to airway smooth muscle cells can attenuate oxidative stress-induced damage in COPD. However, it is not known whether structural cells in the lungs can also exchange mitochondria and what role this plays in modulating bioenergetics and cellular function. Here, researchers found that airway smooth muscle cells in both healthy ex-smokers and COPD patients can exchange mitochondria, partially through extracellular vesicles. Exposure to cigarette smoke induces mitochondrial dysfunction and increases mitochondrial donation by the smooth muscle cells, suggesting it may be a stress response mechanism. Moreover, the study showed that the received mitochondria enhance mitochondrial biogenesis and respiration while reducing cell proliferation in healthy ex-smoker airway smooth muscle cells.
AMERICAN JOURNAL OF RESPIRATORY CELL AND MOLECULAR BIOLOGY
(2022)
Article
Cell Biology
Ruth M. Matthews, Eamonn Bradley, Caoimhin S. Griffin, Xin Rui Lim, Nicolas D. Mullins, Mark A. Hollywood, Fionnuala T. Lundy, Lorcan P. McGarvey, Gerard P. Sergeant, Keith D. Thornbury
Summary: Mouse bronchial smooth muscle cells express functional NaV1.7 channels that can modulate contractile activity under experimental conditions.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Multidisciplinary Sciences
Alina Tokmakova, Donghwa Kim, Brian Guthrie, Soo-Kyung Kim, William A. Goddard, Stephen B. Liggett
Summary: The 25 human bitter taste receptors (TAS2Rs) are an integrated chemosensory system expressed on taste and extra-oral cells. TAS2R14, an archetypal TAS2R, is activated by more than 150 structurally diverse agonists, which raises questions about the accommodation mechanism for these GPCRs. In this study, the researchers computationally derived the structure of TAS2R14 and identified the binding sites and energies for five highly diverse agonists. They found that the binding pocket is the same for all five agonists, and the energetics are consistent with experimental results. The researchers also discovered that TAS2R14 accommodates agonists through unique transmembrane interactions, involving the breaking of a hydrogen bond and the formation of salt bridges.
Review
Biochemistry & Molecular Biology
Luigino Calzetta, Marina Aiello, Annalisa Frizzelli, Giuseppina Bertorelli, Beatrice Ludovica Ritondo, Paola Rogliani, Alfredo Chetta
Summary: AHR is a central pathophysiological feature of asthma, involving ASM with pro-inflammatory and immunomodulatory actions. mAbs target various inflammatory mediators to modulate ASM contractility, showing potential in treating severe asthma by preventing AHR.
Article
Endocrinology & Metabolism
Michael M. Grunstein
Summary: Homeostasis is the process by which the body maintains internal stability within a narrow range. Disrupted homeostasis at the tissue or systemic level leads to disease. In asthma, the pivotal site of disrupted homeostasis is the airway smooth muscle (ASM). This article proposes a novel mechanism in which the pro-asthmatic-exposed ASM independently generates its own active glucocorticoid and uses this molecular strategy to prevent induction of the asthmatic state.
FRONTIERS IN ENDOCRINOLOGY
(2023)
Article
Plant Sciences
Yuting Long, Han Wang, Zitong Ma, Yuman Li, Zaina Ma, Ping Yu, Xiufeng Tang, Renhui Liu
Summary: This study aimed to explore the synergy of Epimedii Folium and Ligustri Lucidi Fructus (ELL) with dexamethasone (Dex) in the proliferation, apoptosis, and autophagy of airway smooth muscle cells (ASMCs). The results of the study suggest that ELL combined with Dex may regulate the proliferation of ASMCs by promoting apoptosis and autophagy and be a potential medicine for the treatment of asthma.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Miguel Angel Hernandez-Lara, Santosh K. Yadav, Sushrut D. Shah, Mariko Okumura, Yuichi Yokoyama, Raymond B. Penn, Taku Kambayashi, Deepak A. Deshpande
Summary: Airway remodeling in asthma involves the hyperproliferation of airway smooth muscle (ASM) cells. The inhibition of diacylglycerol kinase (DGK) reduces the conversion of diacylglycerol (DAG) into phosphatidic acid (PA) and inhibits ASM cell proliferation. These findings suggest that DGK may serve as a potential drug target for mitigating airway remodeling in asthma.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Immunology
Malgorzata Proboszcz, Krzysztof Goryca, Patrycja Nejman-Gryz, Tadeusz Przybylowski, Katarzyna Gorska, Rafal Krenke, Magdalena Paplinska-Goryca
Summary: The study investigated distinct disease profiles based on clinical, cellular, and molecular data from patients with mild-to-moderate obstructive pulmonary diseases. Five key variables were identified to differentiate between asthma and COPD, and cluster analysis revealed two main clusters within the combined samples of asthma and COPD patients.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Review
Physiology
Qin Yang, Wei Shi
Summary: Abnormal airway remodeling in chronic respiratory diseases is characterized by altered proliferation and differentiation of airway smooth muscle cells (ASMCs), which are difficult to restore. Understanding the regulation of airway smooth muscle growth is crucial for identifying intervention targets, including the potentially important RhoA/ROCK-MYOCD/MRTFs pathway.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2021)
Article
Physiology
Shengjie Xu, Nikhil Karmacharya, Gaoyuan Cao, Changjiang Guo, Andrew Gow, Reynold A. Panettieri, Joseph A. Jude
Summary: This article demonstrates the impact of obesity on asthma, showing that it can worsen symptoms through enhanced excitation-contraction coupling in airway smooth muscle cells. The study finds differential metabolic phenotypes between airway smooth muscle cells obtained from obese and nonobese donors, with elevated glycolysis in obese cells. Inhibition of glycolysis attenuates excitation-contraction coupling in airway smooth muscle cells, providing potential therapeutic targets for obesity-related asthma treatment.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Nutrition & Dietetics
Saira Ambreen, Sameen Fatima, Ahmed Elwakiel, Rajiv Rana, Kunal Singh, Anubhuti Gupta, Dheerendra Gupta, Hamzah Khawaja, Jayakumar Manoharan, Christian Besler, Ulrich Laufs, Shrey Kohli, Berend Isermann, Khurrum Shahzad
Summary: Diabetes mellitus is associated with accelerated atherosclerosis and impaired plaque stability, with reduced TM expression and aPC generation. Impaired TM function reduces plaque stability, a characteristic of hyperglycemia-associated plaques, suggesting the crucial role of impaired TM function in mediating diabetes-associated atherosclerosis.
Article
Critical Care Medicine
Annika W. M. Goorsenberg, Julia N. S. D'Hooghe, Karthikan Srikanthan, Nick H. T. Ten Hacken, Els J. M. Weersink, Joris J. T. H. Roelofs, Samuel Kemp, Elisabeth H. Bel, Pallav L. Shah, Jouke T. Annema, Peter Bonta
Summary: The study shows that bronchial thermoplasty can significantly reduce airway smooth muscle mass and treatment response is associated with serum IgE and eosinophil levels, rather than baseline ASM mass.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2021)
Article
Cell Biology
Santosh K. Yadav, Pawan Sharma, Sushrut D. Shah, Reynold A. Panettieri, Taku Kambayashi, Raymond B. Penn, Deepak A. Deshpande
Summary: Inhibition of DGK activates PKA signaling pathway and induces COX expression and PGE(2) production in ASM cells, leading to attenuation of ASM contraction. This novel mechanism suggests DGK as a potential therapeutic target for effective bronchoprotection in asthma.
JOURNAL OF CELLULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Nikita V. Trusov, Vladimir A. . Shipelin, Kristina V. Mzhelskaya, Ntonina A. . Shumakova, Ndrey N. Timonin, Nikolay A. . Riger, Sergey A. . A. Pryatin, Ivan V. Gmoshinski
Summary: The study investigated the effects of resveratrol on muscle tone, anxiety levels, and blood biochemical parameters in obese mice. It was found that resveratrol reduced muscle tone, lowered triglyceride levels, and may have a regulatory effect on triglyceride and cholesterol metabolism.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2021)
Article
Pharmacology & Pharmacy
Kathrin Gerlach, Stefanie Koehler-Bachmann, David Jungck, Sandra Koerber, Sarah Yanik, Heiko Knoop, Deborah Wehde, Sonja Rheinlaender, Joerg W. Walther, Juliane Kronsbein, Juergen Knobloch, Andrea Koch
EUROPEAN JOURNAL OF PHARMACOLOGY
(2015)
Article
Physiology
Juergen Knobloch, Sarah-Jane Chikosi, Sarah Yanik, Jan Rupp, David Jungck, Andrea Koch
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2016)
Article
Pharmacology & Pharmacy
Juergen Knobloch, Sarah Derya Yanik, Sandra Koerber, Erich Stoelben, David Jungck, Andrea Koch
BIOCHEMICAL PHARMACOLOGY
(2016)
Article
Pharmacology & Pharmacy
Juergen Knobloch, Yakup Yakin, Sandra Koerber, Barbara Grensemann, Zeynep Bendella, Niyazi Boyaci, Willem-Jakob Gallert, Sarah Derya Yanik, David Jungck, Andrea Koch
EUROPEAN JOURNAL OF PHARMACOLOGY
(2016)
Article
Biochemical Research Methods
Kathrin E. Witzke, Kristin Rosowski, Christian Mueller, Maike Ahrens, Martin Eisenacher, Dominik A. Megger, Juergen Knobloch, Andrea Koch, Thilo Bracht, Barbara Sitek
JOURNAL OF PROTEOME RESEARCH
(2017)
Article
Physiology
Benjamin Kalbe, Juergen Knobloch, Viola M. Schulz, Christine Wecker, Marian Schlimm, Paul Scholz, Fabian Jansen, Erich Stoelben, Stathis Philippou, Erich Hecker, Hermann Luebbert, Andrea Koch, Hanns Hatt, Sabrina Osterloh
FRONTIERS IN PHYSIOLOGY
(2016)
Article
Allergy
Juergen Knobloch, David Jungck, Catherine Charron, Erich Stoelben, Kazuhiro Ito, Andrea Koch
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2018)
Article
Pharmacology & Pharmacy
Juergen Knobloch, Chiara Wahl, Maria Feldmann, David Jungck, Justus Strauch, Erich Stoelben, Andrea Koch
BASIC & CLINICAL PHARMACOLOGY & TOXICOLOGY
(2014)
Article
Genetics & Heredity
Jurgen Knobloch, Susanne Panek, Sarah Derya Yanik, Kaschin Jamal Jameel, Zeynep Bendella, David Jungck, Paul Buerger, Eike Buelhoff, Birte Struck, Nikolaos Giannakis, Jan Rupp, Juliane Kronsbein, Marcus Peters, Andrea Koch
JOURNAL OF MOLECULAR MEDICINE-JMM
(2019)
Article
Medicine, General & Internal
Juergen Knobloch, David Jungck, Juliane Kronsbein, Erich Stoelben, Kazuhiro Ito, Andrea Koch
JOURNAL OF CLINICAL MEDICINE
(2019)
Article
Environmental Sciences
Juergen Knobloch, Swaantje Casjens, Martin Lehnert, Sarah D. Yanik, Sandra Koerber, Anne Lotz, Jan Rupp, Monika Raulf, Wolfgang Zschiesche, Tobias Weiss, Juliane Kronsbein, Andrea Koch, Thomas Bruening, Beate Pesch
ENVIRONMENTAL RESEARCH
(2020)
Article
Biochemistry & Molecular Biology
Kaschin Jamal Jameel, Willem-Jakob Gallert, Sarah D. Yanik, Susanne Panek, Juliane Kronsbein, David Jungck, Andrea Koch, Jurgen Knobloch
Summary: In smoking-induced COPD, patients with various comorbidities may have suppressed T-cell immunity, leading to a potential explanation for the association with frequent exacerbations. Different biomarkers at varying concentrations can modulate T-cell activation markers, with a clear shift towards suppressive effects, particularly for asthma- and cancer-related comorbidities.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Jonghui Kim, Karla Hegener, Claudia Hagedorn, Kaschin Jamal Jameel, Daniel Weidinger, Inga Marte Charlott Seuthe, Sabine Eichhorn, Florian Kreppel, Juergen Knobloch, Jonas Jae-Hyun Park
Summary: Epithelial cells from cryopreserved tissue showed comparable outgrowth, proliferation, and functionality to that of fresh tissue. This study established a method for long-term cryopreservation of polyp tissue, enabling the isolation and cell culture of primary epithelial cells at a later time. The availability of epithelial cells could be greatly improved by including this method in a biobank.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Medicine, Research & Experimental
J. Knobloch, D. Jungck, A. Koch
CURRENT MOLECULAR MEDICINE
(2017)
Article
Pharmacology & Pharmacy
David Jungck, Juergen Knobloch, Sandra Koerber, Yingfeng Lin, Juergen Konradi, Sarah Yanik, Erich Stoelben, Andrea Koch
JOURNAL OF PHARMACOLOGY AND EXPERIMENTAL THERAPEUTICS
(2015)