Article
Pharmacology & Pharmacy
Henry Danahay, Sarah Lilley, Kathryn Adley, Holly Charlton, Roy Fox, Martin Gosling
Summary: Niclosamide and benzbromarone have been considered as clinical candidates for the treatment of airway diseases due to their activity as inhibitors of the calcium activated chloride channel TMEM16A. However, this study reveals that both compounds have off-target effects and also questions the classification of niclosamide as a TMEM16A blocker. The study further suggests a potential role of TMEM16A in the regulation of epithelial ion transport and mucus secretion in the airway.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Multidisciplinary Sciences
Li Cao, I-Chun Chen, Zhen Li, Xiaowei Liu, Muhammad Mubashir, Reham Al Nuaimi, Zhiping Lai
Summary: This study reports highly crystalline covalent organic framework (COF) membranes that can switch ion selectivity by controlling pH, mimicking neural signal transduction in vivo. The switchable selectivity originates from differentiated coordination interactions between ions and amino acids. These membranes have great potential for biomimetic applications in vitro.
NATURE COMMUNICATIONS
(2022)
Article
Medicine, Research & Experimental
Viktor N. Tomilin, Kyrylo Pyrshev, Anna Stavniichuk, Naghmeh Hassanzadeh Khayyat, Guohui Ren, Oleg Zaika, Sherif Khedr, Alexander Staruschenko, Fang C. Mei, Xiaodong Cheng, Oleh Pochynyuk
Summary: Epac1 and Epac2 have similar but nonredundant roles in renal sodium handling. Inhibition of Epac signaling could be instrumental in the treatment of hypertensive states associated with ENaC overactivation.
Review
Physiology
Wei Zhou, Tong Yu, Yu Hua, Yapeng Hou, Yan Ding, Hongguang Nie
Summary: The balance of gas exchange and lung ventilation is crucial for maintaining body homeostasis. Ion channels and transporters in respiratory epithelial cells play a vital role in maintaining the capacity of liquid layer on the cell surface and providing an immune barrier. However, hypoxia in harmful environments or pathological conditions can disrupt the ion transport function, leading to respiratory diseases. Understanding the relationship between hypoxia and ion transport dysfunction is important for the prevention and treatment of respiratory diseases.
AMERICAN JOURNAL OF PHYSIOLOGY-LUNG CELLULAR AND MOLECULAR PHYSIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Mandy Laube, Ulrich H. Thome
Summary: Albumin enhances epithelial Na+ transport and barrier integrity by activating the PI3K/AKT/SGK1 pathway.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Thamer A. Alsufayan, Evan J. Myers, Bianca N. Quade, Clayton T. Brady, Aniko Marshall, Nayem Haque, Michael E. Duffey, Mark D. Parker
Summary: This study investigated the activity of NBCe1 in the kidneys and colon, as well as the impact of its phosphorylation state on activity. It was found that Ser982 is more phosphorylated in murine kidneys compared to colons. While the phosphorylation status of Ser982 correlates with NBCe1 activity, it is not a key determinant of the transporter stoichiometry.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Biochemistry & Molecular Biology
Lei Zhang, Xueqi Wang, Jingxin Chen, Shaohu Sheng, Thomas R. Kleyman
Summary: Using crosslinking and voltage clamp techniques, we identified specific intersubunit contacts in ENaC that modulate channel activity. Crosslinking between specific residues in the palm and thumb domains of different subunits activated or inhibited ENaC activity. These findings suggest the presence of microswitches at specific subunit interfaces that play a role in ENaC gating and regulation.
JOURNAL OF BIOLOGICAL CHEMISTRY
(2023)
Article
Cell Biology
Shujie Shi, Teresa M. Buck, Andrew J. Nickerson, Jeffrey L. Brodsky, Thomas R. Kleyman
Summary: The mammalian paraoxonases (PONs) play a role in protecting against oxidative stress. Among the PON family members, PON2 and PON3 negatively regulate the expression of ENaC, an ion channel involved in salt and water balance. This study showed that PON2 functions as an ER chaperone to monitor the biogenesis of ENaC and directs the channel for ER-associated degradation, leading to reduced channel surface expression and functional activity.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2022)
Article
Engineering, Chemical
Qing Zhu, Yahua Liu, Peipei Zuo, Yu Dong, Zhengjin Yang, Tongwen Xu
Summary: Researchers have developed a sodium selective isoporous membrane derived from lyotropic liquid crystals, which exhibits excellent Na+ selectivity and ion transport properties. This highly selective membrane could play a crucial role in high precision separation and the design of new generation ion selective membranes.
JOURNAL OF MEMBRANE SCIENCE
(2022)
Article
Urology & Nephrology
Bidisha Saha, Waheed Shabbir, Enzo Takagi, Xin-Peng Duan, Deise Carla Almeida Leite Dellova, John Demko, Anna Manis, Dominique Loffing-Cueni, Johannes Loffing, Mads Vaarby Sorensen, Wen-Hui Wang, David Pearce
Summary: The mTORC2-SGK1-Nedd4-2-ENaC signaling axis plays a crucial role in the rapid renal responses to increased plasma potassium concentration. Other downstream targets of this signaling pathway, such as PKC and Akt, are not acutely affected, and ROMK and BK channels are not activated. These findings provide new insights into the signaling network and ion transport systems underlying renal responses to potassium in vivo.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Peripheral Vascular Disease
Brandon M. Blobner, Annet Kirabo, Ossama B. Kashlan, Shaohu Sheng, Donna K. Arnett, Lewis C. Becker, Eric Boerwinkle, Jenna C. Carlson, Yan Gao, Richard A. Gibbs, Jiang He, Marguerite R. Irvin, Sharon L. R. Kardia, Tanika N. Kelly, Charles Kooperberg, Stephen T. McGarvey, Vipin K. Menon, May E. Montasser, Take Naseri, Susan Redline, Alexander P. Reiner, Muagututi'a S. Reupena, Jennifer A. Smith, Xiao Sun, Dhananjay Vaidya, Karine A. Viaud-Martinez, Daniel E. Weeks, Lisa R. Yanek, Xiaofeng Zhu, Ryan L. Minster, Thomas R. Kleyman
Summary: This study found an association between low-frequency and rare variants in ENaC subunits and blood pressure and kidney function.
Article
Biochemistry & Molecular Biology
Stefano Stabilini, Anna Menini, Simone Pifferi
Summary: TMEM16F is involved in various physiological processes and acts as both a Ca2+-dependent phospholipid scramblase and a Ca2+-activated ion channel. The ion selectivity of TMEM16F-mediated current depends on the recording configuration, with higher permeability for Na+ in inside-out experiments and a slight preference for Cl over bar permeation in whole-cell recordings.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Neurosciences
Sabrina Noel, Nathalie Servel, Aurelie Hatton, Anita Golec, Mayuree Rodrat, Demi R. S. Ng, Hongyu Li, Iwona Pranke, Alexandre Hinzpeter, Aleksander Edelman, David N. Sheppard, Isabelle Sermet-Gaudelus
Summary: This study investigates the relationship between genotype, phenotype, and CFTR function using human nasal epithelial cells, revealing differences in CFTR-mediated currents based on residual or minimal CFTR function. The findings suggest that CFTR expression and function are not affected by conditional reprogramming culture, and highlight the correlation between CFTR-mediated Cl- currents in hNE cells and sweat Cl- concentration, emphasizing the value of these cells in CFTR research and therapeutic testing.
JOURNAL OF PHYSIOLOGY-LONDON
(2022)
Review
Biochemistry & Molecular Biology
Oscar Brenes, Michael Pusch, Fernando Morales
Summary: Myotonia congenita is a hereditary muscle disease caused by mutations in the CLCN1 gene, leading to abnormal function of the chloride ion channel and resulting in muscle hyperexcitability and other symptoms. This review examines over 350 reported mutations and analyzes their effects on the structure and function of the chloride ion channel.
Review
Neurosciences
Stefan Gruender, Audrey Ortega Ramirez, Gaspar Jekely
Summary: DEG/ENaCs interact with neuropeptides via different structural bases and origins, where RFamide neuropeptides activate FaNaCs in invertebrates and modulate ASICs in vertebrates. Dynorphin opioid peptides, on the other hand, stabilize the closed state of ASICs. Despite similar peptide sequences, peptides interacting with DEG/ENaCs from different phyla are evolutionarily unrelated to each other.
JOURNAL OF PHYSIOLOGY-LONDON
(2023)
Article
Oncology
Keita Katsurahara, Atsushi Shiozaki, Toshiyuki Kosuga, Michihiro Kudou, Katsutoshi Shoda, Tomohiro Arita, Hirotaka Konishi, Shuhei Komatsu, Takeshi Kubota, Hitoshi Fujiwara, Kazuma Okamoto, Mitsuo Kishimoto, Eiichi Konishi, Yoshinori Marunaka, Eigo Otsuji
ANNALS OF SURGICAL ONCOLOGY
(2020)
Review
Cell Biology
Atsushi Shiozaki, Yoshinori Marunaka, Eigo Otsuji
Summary: Ion and water channels play a role in the function of cancer cells, including esophageal and gastric cancer cells. The expression and regulation of these channels and transporters could be potential targets for cancer management.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Review
Biochemistry & Molecular Biology
Yoshinori Marunaka
Summary: Type 2 diabetes mellitus is a common lifestyle-related disease caused by overeating and lack of exercise, leading to metabolic disorders. Hyperglycemia and insulin resistance are main symptoms, with the instability of interstitial fluid pH potentially affecting insulin binding affinity.
BIOCHEMICAL SOCIETY TRANSACTIONS
(2021)
Article
Biophysics
Shigekuni Hosogi, Yoshinori Marunaka, Eishi Ashihara, Tadaaki Yamada, Ayumi Sumino, Hideo Tanaka, Leonardo Puppulin
Summary: A newly designed nanosensor anchored to the outer cell surface can quantify H2O2 at nanometer distance, providing important information for studying cell signaling. The nanosensor is built upon gold nanoparticles functionalized with a H2O2-sensitive boronate compound and probed using surface enhanced Raman spectroscopy (SERS).
BIOSENSORS & BIOELECTRONICS
(2021)
Article
Biochemistry & Molecular Biology
Yoshinori Marunaka, Katsumi Yagi
Summary: This study investigates the intracellular protein trafficking processes using mathematical equations, exploring the implications of real and complex roots on the protein quantities in different states. Real roots correspond to monotonic changes, while complex roots lead to oscillatory changes, indicating the essential role of complex numbers in oscillatory phenomena in bodily and cellular functions.
COMPUTATIONAL AND STRUCTURAL BIOTECHNOLOGY JOURNAL
(2021)
Article
Physiology
Saori Tanaka, Shigenori Ito, Chikao Shimamoto, Hitoshi Matsumura, Toshio Inui, Yoshinori Marunaka, Takashi Nakahari
Summary: This study demonstrates that NO production is enhanced by AA via PPAR alpha in ACh-stimulated antral mucous cells, and the production of AA is regulated by cPLA2 activation. Additionally, indomethacin enhances Ca2+-regulated exocytosis by increasing NO synthesis through PPAR alpha activation.
EXPERIMENTAL PHYSIOLOGY
(2021)
Article
Endocrinology & Metabolism
Wataru Aoi, Masayo Iwasa, Yoshinori Marunaka
Summary: Dietary flavonoid intake is associated with the regulation of nutrient metabolism and has been shown to lower the risk of metabolic and cardiovascular diseases. Studies have also demonstrated that flavonoids can improve energy expenditure and glucose tolerance. Furthermore, some flavonoids may regulate thermogenesis and feeding behavior through modulating the autonomic and central nervous systems.
Article
Biochemistry & Molecular Biology
Yoshinori Marunaka, Katsumi Yagi, Noboru Imagawa, Hironori Kobayashi, Masaru Murayama, Asami Minamibata, Yoshiaki Takanashi, Takashi Nakahari
Summary: The study suggests that serum Cl- concentration may be a more effective marker for detecting metabolic conditions compared to HCO3- concentration. Cl- concentration is positively correlated with age and negatively correlated with FBS and HbA1c, especially in individuals with high FBS/HbA1c levels.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cell Biology
Kotoku Kawaguchi, Shogo Nakayama, Daichi Saito, Haruka Kogiso, Kasane Yasuoka, Yoshinori Marunaka, Takashi Nakahari, Shinji Asano
Summary: Mucociliary clearance is a major defense mechanism of the airway epithelium, and ezrin plays an important role in regulating the beating of airway ciliary cells by promoting the apical surface localization of β(2)AR.
JOURNAL OF CELL SCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Yuko Kuwahara, Kohei Takahashi, Miho Akai, Ikuo Kato, Takaharu Kozakai, Shinji Asano, Toshio Inui, Yoshinori Marunaka, Atsukazu Kuwahara
Summary: Xenin-25 has various physiological functions in the gastrointestinal tract, including ion transport and motility. It shows sequence homology with neurotensin, and the minimal biological domain required for inducing ion transport in the rat ileum contains the C-terminal pentapeptide. Furthermore, Arg at position 21 is crucial for retaining the biological activity of Xenin-25.
Article
Multidisciplinary Sciences
Shigeru Shinomoto, Yasuhiro Tsubo, Yoshinori Marunaka
Summary: Cardiac disorders are common and serious, so continuous monitoring of cardiac status is crucial. A newly developed automated system for alerting individuals about cardiac disorders solely by measuring heart periods is described in this paper. The study found that local variation metric is more efficient than conventional heart rate variability metrics for alerting cardiac disorders, and a newly introduced local-global variation metric has superior capacity for discriminating between different types of cardiac problems.
SCIENTIFIC REPORTS
(2022)
Article
Environmental Sciences
Mami Ishida, Keiichi Matsuzaki, Hitoshi Suzuki, Yusuke Suzuki, Takashi Kawamura, Yoshinori Marunaka, Taku Iwami
Summary: Persistent hematuria is associated with the presence of hematuria and proteinuria suggestive of glomerulonephritis, but not with eGFR deterioration. Higher frequency of preceding hematuria is associated with a greater risk of hematuria 5 years later.
INTERNATIONAL JOURNAL OF ENVIRONMENTAL RESEARCH AND PUBLIC HEALTH
(2022)
Review
Biochemistry & Molecular Biology
Yoshinori Marunaka
Summary: Insulin resistance is a factor in the development of type 2 diabetes and Alzheimer's disease. Understanding the mechanisms of insulin resistance is crucial for preventing these diseases. The body's pH environment plays a role in regulating hormone action and maintaining homeostasis. Diet therapies can improve insulin resistance by raising the pH of interstitial fluid with weak organic acids and promoting their absorption in the gut.
Review
Physiology
Yoshinori Marunaka
Summary: This article reviews the various roles of Cl- in the body, including its involvement in cellular functions, cell proliferation, taste receptors, ciliary motility, and its relationship with H+.
JOURNAL OF PHYSIOLOGICAL SCIENCES
(2023)
Article
Physiology
Yuko Kuwahara, Ikuo Kato, Toshio Inui, Yoshinori Marunaka, Atsukazu Kuwahara
Summary: Xenin25 has an inhibitory effect on the spontaneous circular muscle contractions in the rat distal colon, with postinhibitory spontaneous contractions at a higher frequency. The mechanism involves activation of neuronal NTSR1, transmitters from VIP and apamin-sensitive NO neurons, and NK1R to modulate the muscle contractions. Xenin25 is considered an important modulator of postprandial circular muscle contraction in the distal colon.
PHYSIOLOGICAL REPORTS
(2021)
