Journal
JOURNAL OF PHARMACOLOGICAL SCIENCES
Volume 110, Issue 3, Pages 245-250Publisher
JAPANESE PHARMACOLOGICAL SOC
DOI: 10.1254/jphs.09R03FM
Keywords
allergic dermatitis; histamine; picryl chloride; 12-O-tetradecanoylphorbol 13-acetate; thymic stromal lymphopoietin; allergic inflammation
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We established a novel allergic dermatitis model I'll Mouse ear lobes in which antigen-nonspecific inflammation was induced by painting 12-O-tetradecanoylphorbol 13-acetate (TPA) between sensitization and challenge with picryl chloride (PiCl). Tills model has all advantage for analyzing atopic dermatitis-like inflammation within a short period. Analysis of the time Course changes in the PiCl-induced swelling showed that the allergic Inflammation was shifted from a delayed-type response to a biphasic response consisting of a weak immediate-phase response and a late-phase response by painting with TPA. The application of TPA increased the PiCl-induced infiltration of eosinophils and mast cells at the inflammatory site and shifted the cytokine milieu from Th1 to Th2. The expression of the Th2-inducing cytokine thymic stromal lymphopoietin (TSLP) mRNA was also increased by TPA. These findings suggested that the induction of antigen-nonspecific inflammation by TPA before the antigen challenge enhanced the Th2 response and modified the PiCl-induced delayed type-hypersensitivity. Using this model, we clarified that histamine plays significant roles in the early-phase swelling via H-1 receptors and the late-phase swelling via H-3/H-4 receptors. Thus, we Suggested the usefulness of the combined treatment with an H, antagonist and an H-4 antagonist for the suppression of atopic dermatitis.
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