Journal
JOURNAL OF PHARMACEUTICAL SCIENCES
Volume 100, Issue 5, Pages 1637-1642Publisher
WILEY-BLACKWELL
DOI: 10.1002/jps.22407
Keywords
gene delivery; plasmid DNA; cell-penetrating peptides; A549 cells; stability; nanoparticles; stability; cytotoxicity
Funding
- NCRR NIH HHS [P20 RR016443] Funding Source: Medline
- NIAMS NIH HHS [R03 AR054035] Funding Source: Medline
- NIGMS NIH HHS [T32 GM008359, T32 GM08359-11] Funding Source: Medline
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Drug delivery strategies using cell-penetrating peptides (CPPs) have been widely explored to improve the intracellular delivery of a large number of cargo molecules. Electrostatic complexation of plasmid DNA using CPPs has been less explored due to the relatively large complexes formed and the low levels of gene expression achieved when using these low-molecular-weight polycations as DNA condensing agents. Here, condensing nascent CPP polyplexes using CaCl2 produced small and stable nanoparticles leading to gene expression levels higher than observed for control polyethylenimine gene vectors. This simple formulation approach showed negligible cytotoxicity in A549 lung epithelial cells and maintained particle size and transfection efficiency even in the presence of serum. (C) 2010Wiley-Liss, Inc. and the American Pharmacists Association J Pharm Sci 100:1637-1642, 2011
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