Journal
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
Volume 88, Issue -, Pages 423-428Publisher
ELSEVIER
DOI: 10.1016/j.jpba.2013.09.028
Keywords
Needle extraction; Breath analysis; Very volatile organic compounds; Sample preparation; Gas chromatography
Categories
Funding
- Japan Society for the Promotion of Science (JSPS)
- Grants-in-Aid for Scientific Research [23500779, 24651187, 24612002] Funding Source: KAKEN
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A novel needle-type sample preparation device was developed for the determination of very volatile organic compounds (VVOCs) in gaseous samples by gas chromatography-mass spectrometry (GC-MS). Two types of activated-carbon-based sorbents, Carbopack X and a carbon molecular sieve (CMS), were investigated as the extraction medium. A double-bed-type extraction needle showed successful extraction and desorption performance for all investigated VVOCs, including acetaldehyde, isoprene, pentane, acetone, and ethanol. Sensitive and reliable determination of VVOCs was achieved by systematically optimizing several desorption conditions. In addition, the effects of sample humidity on the extraction and desorption of analytes were investigated with the needle-type extraction devices. Only the CMS packed extraction needle was adversely affected by sample humidity during the desorption process; on the other hand the double-bed-type extraction needle was unaffected by sample humidity. Finally, the developed double-bed-type extraction needle was successfully applied to the analysis of breath VVOCs of healthy subjects. (C) 2013 Elsevier B.V. All rights reserved.
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