Article
Materials Science, Multidisciplinary
Bo Kyeong Yoon, Tun Naw Sut, Ki Yeol Yoo, Seung Hwa Lee, Youngkyu Hwang, Joshua A. Jackman, Nam-Joon Cho
Summary: A streamlined ELISA strategy using lipid nanoparticles as a blocking agent was developed, showing fewer processing steps, quicker protocol time, and superior detection performance compared to conventional BSA blocking. This method has been successfully applied to COVID-19 antibody detection and is broadly applicable for rapid ELISA diagnostics development.
APPLIED MATERIALS TODAY
(2021)
Article
Multidisciplinary Sciences
Youhee Heo, Kwanwoo Shin, Min Cheol Park, Ji Yoon Kang
Summary: This report introduces a method for enhancing the sensitivity of chemifluorescence-based ELISA, using photooxidation-induced fluorescence amplification (PIFA). PIFA utilizes autocatalytic photooxidation to amplify the fluorescent product, improving detection sensitivity. The method requires minimal modifications to the fluorescence reader and is compatible with conventional ELISA assays.
SCIENTIFIC REPORTS
(2021)
Article
Chemistry, Analytical
Jie Fei, Jiaqun Jiang, Yuchen Bai, Weilin Wu, Xiangjun Zhao, Wenbo Yu, Kai Wen, Xuezhi Yu, Jianzhong Shen, Zhanhui Wang
Summary: This study demonstrates the importance of careful hapten design and extensive antibody screening in the development of sandwich immunoassays for small molecules, using ABM as an example. Through combinatorial associations of antibody pairs, a sandwich immunoassay for ABM was successfully established.
ANALYTICAL CHEMISTRY
(2023)
Article
Chemistry, Applied
Long Xu, Fanfan Yang, Alberto C. P. Dias, Xiaoying Zhang
Summary: Sunset yellow is a commonly used food colorant, but excessive and illegal use may pose health risks. Researchers have applied quantum dots in the analysis of sunset yellow and developed two new detection methods. These methods are efficient, highly sensitive, and capable of distinguishing sunset yellow from analogues.
Article
Chemistry, Analytical
Hui Li, Menglu Li, Yucai Yang, Fei Wang, Feng Wang, Chao Li
Summary: A direct method has been described to establish the correlation between non-nucleic acid analytes and the CRISPR/Cas12a system using rationally designed DNA strands, enabling ultrasensitive detection of biomarkers from different species. The signal output is highly optional and the sensing principle is akin to ELISA, allowing for direct application on the currently available ELISA platform, further facilitating its use in medical diagnostics.
ANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Jijing Wang, Susanna L. Lundstrom, Sven Seelow, Sergey Rodin, Zhaowei Meng, Juan Astorga-Wells, Qinyu Jia, Roman A. Zubarev
Summary: A novel monoclonal antibody 1A3 with excellent specificity to isoAsp in the functionally important domain of HSA was developed, leading to the quantification of isoAsp occupancy in 100 healthy plasma samples. These results suggest the potential of isoAsp measurements for supplementary AD diagnostics and assessing the freshness of stored donor blood for transfusion suitability.
Article
Biochemical Research Methods
Supaluk Krittanai, Rattanathorn Choonong, Khunkhang Butdapheng, Jakkrit Jabsanthia, Seiichi Sakamoto, Waraporn Putalun
Summary: This study aimed to develop an enzyme-linked immunosorbent assay (ELISA) using a glabridin-specific antibody. The glabridin-bovine serum albumin conjugates were injected into mice to produce hybridomas, and a highly specific antibody against glabridin was produced. The developed ELISA method showed high sensitivity and specificity for quantifying glabridin in plant materials and human serum samples.
PHYTOCHEMICAL ANALYSIS
(2023)
Article
Chemistry, Multidisciplinary
Jianlei Shen, Bo Situ, Xianchao Du, Zhiming Wang, Rong Hu, Baixue Li, Anjun Qin, Ben Zhong Tang
Summary: This study presents a strategy to improve the detection sensitivity and broaden the dynamic range of enzyme-linked immunosorbent assay (ELISA) by replacing traditional chromogenic reagents with an aggregation-induced emission luminogen (AIEgen). The developed AIE-ELISA showed a lower limit of detection and a broader dynamic range compared to single-mode ELISA in the detection of prostate-specific antigen (PSA), allowing for differentiation of minor PSA differences in patient's serum. The AIE-ELISA demonstrated simpler experimental operation, faster enzyme response speed, and better photostability than traditional chromogenic reagents, making it highly promising in the fields of immunoassay, immunohistochemistry, and immunocytochemistry.
Article
Food Science & Technology
Xiya Zhang, Mingyue Ding, Chensi Zhang, Yexuan Mao, Youyi Wang, Peipei Li, Haiyang Jiang, Zhanhui Wang, Xuezhi Yu
Summary: The researchers developed a rapid icELISA method for monitoring brevetoxin levels in oysters, providing a useful tool for the detection of these toxins in shellfish samples.
Article
Chemistry, Analytical
Hinata Maeno, Satoshi Ogata, Tetsuhide Shimizu, Ming Yang
Summary: The authors have developed a film-stacked structure using polyethylene terephthalate (PET) films, which can be combined with 96-well microplates for biochemical analysis. By inserting this structure into a well and rotating it, convection flow is generated to enhance chemical reactions between molecules. However, only a part of the solution circulates into the narrow gaps between the films, resulting in lower reaction efficiency. In this study, unsteady rotation is applied to improve analyte transport into the gaps using secondary flow generated on the rotating disk surface. Finite element analysis is used to optimize the rotation conditions and evaluate the molecular binding ratio.
Article
Plant Sciences
Lucia Vieira Hoffmann, Amanda Alves Branquinho, Paulo Augusto Vianna Barroso, Maite F. S. Vaslin
Summary: By developing monoclonal and polyclonal antibodies based on coat protein peptides, an efficient ELISA detection method was established to detect the cotton blue disease virus in cotton and weeds. The study has the potential to guide the development of other diagnostic procedures.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Parasitology
Phattharaphon Wongphutorn, Chanika Worasith, Kulthida Y. Kopolrat, Chutima Homwong, Jiraporn Sithithaworn, Chatanun Eamudomkarn, Anchalee Techasen, Patcharaporn Tippayawat, Opal Pitaksakurat, Nuttanan Hongsrichan, Thomas Crellen, Paiboon Sithithaworn
Summary: Detection of specific IgG4 in urine can achieve similar diagnostic accuracy to serum for strongyloidiasis. This suggests that urine IgG4 can be used as a valid diagnostic marker.
PARASITES & VECTORS
(2023)
Article
Chemistry, Applied
Shunsuke Fujii, Tomoe Ohta, Riho Ehama, Mizuki Irikida, Shuichi Nomura, Yukihiro Shoyama, Takuhiro Uto
Summary: This study developed a simple and reliable method for the detection and quantification of FMN in food samples, and successfully applied it in cell-based assays.
Article
Biochemistry & Molecular Biology
Masamitsu Harada, Jun Nagai, Riho Kurata, Xiaofeng Cui, Takayuki Isagawa, Hiroaki Semba, Yasuhiro Yoshida, Norihiko Takeda, Koji Maemura, Tomo Yonezawa
Summary: The RE-1 silencing factor is a fundamental repressor element in regulating neuronal genes, interacting with Sin3B to maintain neuronal robustness. Through surface plasmon resonance, a direct interaction and competitive binding of two PAH1 peptides were confirmed. A novel high-quality TR-FRET assay and an interaction assay based on SPR were successfully established.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Agriculture, Dairy & Animal Science
Aicha Yasmine Bellatreche, Riad Bouzid, Amandine Blaizot, Dominique Aubert, Radu Blaga, Khatima Ait-Oudhia, Delphine Le Roux
Summary: Toxoplasmosis is a zoonotic disease caused by the protozoan Toxoplasma gondii, affecting most warm-blooded animals. Assessing the seroprevalence of T. gondii in different animal species gives a good estimate of the global circulation of the parasite and the risk for human infections. This study compared two methods for detecting T. gondii antibodies in hunting dogs and found a high prevalence of infection. It emphasizes the importance of studying T. gondii infections in companion animals to assess the risk for human populations.
Article
Chemistry, Multidisciplinary
Xiaozhou Luo, Tao Liu, Ying Wang, Haiqun Jia, Yuhan Zhang, Dawna Caballero, Juanjuan Du, Rongsheng E. Wang, Danling Wang, Peter G. Schultz, Feng Wang
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2015)
Article
Chemistry, Multidisciplinary
Yu Cao, Jun Y. Axup, Jennifer S. Y. Ma, Rongsheng E. Wang, Seihyun Choi, Virginie Tardif, Reyna K. V. Lim, Holly M. Pugh, Brian R. Lawson, Gus Welzel, Stephanie A. Kazane, Ying Sun, Feng Tian, Shailaja Srinagesh, Tsotne Javahishvili, Peter G. Schultz, Chan Hyuk Kim
ANGEWANDTE CHEMIE-INTERNATIONAL EDITION
(2015)
Article
Chemistry, Multidisciplinary
Tao Liu, Guangsen Fu, Xiaozhou Luo, Yan Liu, Ying Wang, Rongsheng E. Wang, Peter G. Schultz, Feng Wang
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2015)
Article
Chemistry, Multidisciplinary
Rongsheng E. Wang, Tao Liu, Ying Wang, Yu Cao, Jintang Du, Xiaozhou Luo, Vishal Deshmukh, Chan Hyuk Kim, Brian R. Lawson, Matthew S. Tremblay, Travis S. Young, Stephanie A. Kazane, Feng Wang, Peter G. Schultz
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2015)
Editorial Material
Biotechnology & Applied Microbiology
Renhe Liu, Rongsheng E. Wang, Feng Wang
EXPERT OPINION ON BIOLOGICAL THERAPY
(2016)
Article
Multidisciplinary Sciences
Xiaozhou Luo, Claudio Zambaldo, Tao Liu, Yuhan Zhang, Weimin Xuan, Chen Wang, Sean A. Reed, Peng-Yu Yang, Rongsheng E. Wang, Tsotne Javahishvili, Peter G. Schultz, Travis S. Young
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Multidisciplinary Sciences
Rongsheng E. Wang, Ying Wang, Yuhan Zhang, Chase Gabrelow, Yong Zhang, Victor Chi, Qiangwei Fu, Xiaozhou Luo, Danling Wang, Sean Joseph, Kristen Johnson, Arnab K. Chatterjee, Timothy M. Wright, Van T. B. Nguyen-Tran, John Teijaro, Argyrios N. Theofilopoulos, Peter G. Schultz, Feng Wang
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2016)
Article
Biochemistry & Molecular Biology
Rongsheng E. Wang, Raj K. Pandita, Jianfeng Cai, Clayton R. Hunt, John-Stephen Taylor
Article
Medicine, Research & Experimental
R. E. Wang, Y. Zhang, L. Tian, W. Cai, J. Cai
CURRENT MOLECULAR MEDICINE
(2013)
Review
Chemistry, Multidisciplinary
Rongsheng E. Wang, Frankie Costanza, Youhong Niu, Haifan Wu, Yaogang Hu, Whitney Hang, Yiqun Sun, Jianfeng Cai
JOURNAL OF CONTROLLED RELEASE
(2012)
Article
Medicine, Research & Experimental
Youhong Niu, Ge Bai, Haifan Wu, Rongsheng E. Wang, Qiao Qiao, Shruti Padhee, Robert Buzzeo, Chuanhai Cao, Jianfeng Cai
MOLECULAR PHARMACEUTICS
(2012)
Article
Chemistry, Organic
Ge Bai, Shruti Padhee, Youhong Niu, Rongsheng E. Wang, Qiao Qiao, Robert Buzzeo, Chuanhai Cao, Jianfeng Cai
ORGANIC & BIOMOLECULAR CHEMISTRY
(2012)
Article
Oncology
Rongsheng E. Wang, Youhong Niu, Haifan Wu, Yaogang Hu, Jianfeng Cai
ANTI-CANCER AGENTS IN MEDICINAL CHEMISTRY
(2012)
Article
Biochemical Research Methods
Ling Tian, Rongsheng E. Wang, Ying Fei, Yie-Hwa Chang
JOURNAL OF BIOMOLECULAR SCREENING
(2012)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)