Review
Oncology
Sherrie Mikhaeel, Ehab Atallah
Summary: Tyrosine kinase inhibitor (TKI) discontinuation, also known as treatment-free remission (TFR), is an important goal in the therapy of chronic myeloid leukemia (CML) due to the impact of TKI therapy on quality of life, long-term side effects, and financial burden. TKI discontinuation is particularly crucial for young CML patients as it affects their growth and development. Multiple studies have demonstrated the safety and feasibility of attempting TKI discontinuation in patients with sustained deep molecular remission.
CLINICAL LYMPHOMA MYELOMA & LEUKEMIA
(2023)
Article
Biochemical Research Methods
Yao Zhang, Yao Liu, Lu Tan
Summary: A UHPLC-MS/MS bioassay for simultaneous determination of methotrexate, imatinib and dasatinib has been developed and fully validated, showing high precision and accuracy in therapeutic drug monitoring.
Review
Oncology
Kota Yoshifuji, Koji Sasaki
Summary: The prognosis of CML-CP has significantly improved with the introduction of imatinib. Besides imatinib, other TKIs and a novel drug asciminib are now available. Long-term TKI therapy requires individualized selection based on toxicity profile to minimize chronic toxicity and the risk of adverse events. This review summarizes the characteristics and adverse event profile of each TKI and discusses future perspectives in the treatment of CML-CP.
FRONTIERS IN ONCOLOGY
(2022)
Article
Mathematical & Computational Biology
Yuji Okamoto, Mitsuhito Hirano, Kai Morino, Masashi K. Kajita, Shinji Nakaoka, Mayuko Tsuda, Kei-ji Sugimoto, Shigehisa Tamaki, Junichi Hisatake, Hisayuki Yokoyama, Tadahiko Igarashi, Atsushi Shinagawa, Takeaki Sugawara, Satoru Hara, Kazuhisa Fujikawa, Seiichi Shimizu, Toshiaki Yujiri, Hisashi Wakita, Kaichi Nishiwaki, Arinobu Tojo, Kazuyuki Aihara
Summary: This study successfully classified CML patients with different nilotinib responses by using a dynamical model and laboratory findings. A method was proposed to identify patients who failed to achieve a treatment goal based on data collected at three initial time points only.
NPJ SYSTEMS BIOLOGY AND APPLICATIONS
(2022)
Review
Oncology
Theresa Steeb, Anja Wessely, Anne Petzold, Christoph Kohl, Michael Erdmann, Carola Berking, Markus V. Heppt
Summary: The study summarized the efficacy and safety of c-Kit inhibitors for unresectable or metastatic mucosal, acral or chronically sun-damaged melanoma. Results showed that c-Kit inhibitors are a valuable treatment option for patients with KIT mutant melanoma, with nilotinib having the highest objective response rate.
EUROPEAN JOURNAL OF CANCER
(2021)
Article
Hematology
Fadi G. Haddad, Koji Sasaki, Ghayas C. Issa, Guillermo Garcia-Manero, Farhad Ravandi, Tapan Kadia, Jorge Cortes, Marina Konopleva, Naveen Pemmaraju, Yesid Alvarado, Musa Yilmaz, Gautam Borthakur, Courtney DiNardo, Nitin Jain, Naval Daver, Nicholas J. Short, Elias Jabbour, Hagop Kantarjian
Summary: This study evaluated the outcome of TKI discontinuation in CML patients and identified factors associated with success rates of treatment-free remission (TFR). The study found that TFR is safe and feasible in patients with Ph-positive CML on TKI therapy. Achieving MR4 or MR4.5 for at least 5 years was correlated with a better outcome.
AMERICAN JOURNAL OF HEMATOLOGY
(2022)
Article
Pharmacology & Pharmacy
R. Ciftciler, I. C. Haznedaroglu
Summary: Philadelphia (Ph*)/BCR-A BL1-positive chronic myeloid leukemia (CML) is a neoplastic hematologic disorder that can be functionally cured using tyrosine kinase inhibitor (TKI) drugs. Factors such as individual characteristics, drug compliance, lifestyle preferences, comorbidities, and physician experience should be considered when selecting the appropriate first-line TKI for newly diagnosed CML patients.
EUROPEAN REVIEW FOR MEDICAL AND PHARMACOLOGICAL SCIENCES
(2021)
Article
Agriculture, Multidisciplinary
Jianhua Wang, Minmin Ge, Lirui Sun, Ishfaq Ahmed, Wenjie Li, Hang Lin, Hong Lin, Zhenxing Li
Summary: A new method for the qualitative and quantitative detection of crustacean tropomyosin in various food matrices was developed, showing accuracy and reliability.
JOURNAL OF THE SCIENCE OF FOOD AND AGRICULTURE
(2021)
Review
Oncology
Takaaki Ono
Summary: This review discusses the importance of selecting the optimal BCR-ABL1 tyrosine kinase inhibitors (TKIs) for newly diagnosed CML-CP patients, and how individualized treatment choices can be made based on factors such as patient background, treatment goals, and cost.
Article
Hematology
Sean Tracy, Hrishi Venkatesh, Can Hekim, Lynn M. Heltemes-Harris, Todd P. Knutson, Veronika Bachanova, Michael A. Farrar
Summary: Research on acute lymphoblastic leukemia patients has shown that CD4(+) T cell exhaustion may be a key factor in relapse. Combined treatment with tyrosine kinase inhibitors and anti-PD-L1 significantly improves patients’ survival rates. Depleting CD4(+) T cells prior to therapy may weaken the immune response, while treatment with anti-PD-L1 helps activate the anti-leukemia response.
Article
Medicine, Research & Experimental
Massimo Breccia, Francesca Chiodi, Aurelio Pio Nardozza, Diletta Valsecchi, Valentina Perrone, Diego Sangiorgi, Elisa Giacomini, Maria Chiara Rendace, Paola Coco, Eleonora Premoli, Luca Degli Esposti
Summary: This study analyzed the economic burden for managing patients with chronic myeloid leukemia (CML) in 2nd or > 3rd lines with tyrosine kinase inhibitors (TKIs) in Italian clinical practice. The results showed that as the treatment lines increased, healthcare consumption and costs for CML patients also increased, with hospitalizations accounting for about half of the total expenditure.
ADVANCES IN THERAPY
(2023)
Article
Environmental Sciences
Jingrong He, Li Li, Yunxiang Li, Mei Huang, Ying Zhu, Shihuai Deng
Summary: In this study, six aromatic organosulfates were synthesized and their purity and concentration were determined using high performance liquid chromatography/ultraviolet detector and high performance liquid chromatography/electrospray ionization/tandem mass spectrometry. The characteristic ion fragments were found to improve the identification and detection of target compounds.
ATMOSPHERIC ENVIRONMENT
(2022)
Article
Hematology
Yves Chalandon, Giulia Sbianchi, Luuk Gras, Linda Koster, Jane Apperley, Jenny Byrne, Urpu Salmenniemi, Henrik Sengeloev, Mahmoud Aljurf, Grzegorz Helbig, Francesca Kinsella, Goda Choi, Peter Remenyi, John A. Snowden, Marie Robin, Stig Lenhoff, Stephan Mielke, Jakob Passweg, Annoek E. C. Broers, Nicolaus Kroeger, Zeynep Arzu Yegin, Sen Mui Tan, Patrick J. Hayden, Donal P. McLornan, Ibrahim Yakoub-Agha
Summary: With the introduction of TKI, the number of allo-HCT for CP CML patients has decreased significantly. The use of 1, 2, or 3 TKI prior to allo-HCT does not affect the overall survival, progression-free survival, relapse incidence, or non-relapse mortality. However, the disease stage and KPS score significantly influence the survival and progression-free survival, emphasizing the importance of careful candidate selection and performing allo-HCT during CP1 phase.
AMERICAN JOURNAL OF HEMATOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Vanesa Escudero-Ortiz, Francisco Jose Rodriguez-Lucena, Gabriel Estan-Cerezo, Esther Mancheno-Macia, Venancio Conesa-Garcia, Ana Garcia-Monsalve, Leticia Soriano-Irigaray, Andres Navarro-Ruiz
Summary: This study aimed to develop and validate a selective and precise high performance liquid chromatography-ultraviolet method for the measurement of nilotinib in plasma from patients with chronic myeloid leukemia. The method demonstrated sensitivity, selectivity, reproducibility, and rapidity, making it suitable for pharmacokinetic studies and therapeutic drug monitoring in routine clinical practice.
Article
Chemistry, Analytical
He-Ying Liu, Qi-Zhen Cheng, Hui-Zheng Fu, Zhen-Hua Zhong, Hong-Ying Xia, Yan-Fang Guo, Shuang-Shuang Zheng, Shi-Shi Yu
Summary: In this study, five impurities in cloperastine hydrochloride bulk drug were isolated and identified, and a quantitative HPLC method was developed for detection. The structures of these impurities were characterized using various spectral data, with some identified as new isomers or intermediates of cloperastine.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Medicine, General & Internal
Jessica Cusato, Valeria Avataneo, Miriam Antonucci, Mattia Trunfio, Letizia Marinaro, Alice Palermiti, Alessandra Manca, Giovanni Di Perri, Jacopo Mula, Stefano Bonora, Antonio D'Avolio, Andrea Calcagno
Summary: Neurocognitive impairments are common in people living with HIV, and various factors, including host genetic variants and inflammatory conditions, can affect the penetration of antiretroviral drugs in the cerebrospinal fluid. This study aimed to evaluate the penetration of antiretroviral drugs in the cerebrospinal fluid based on compartmental inflammation, blood-brain barrier permeability, and genetic variants in drug transporter encoding genes.
Article
Biochemistry & Molecular Biology
Jessica Cusato, Alessandra Manca, Alice Palermiti, Jacopo Mula, Martina Costanzo, Miriam Antonucci, Francesco Chiara, Elisa Delia De Vivo, Domenico Maiese, Micol Ferrara, Stefano Bonora, Giovanni Di Perri, Antonio D'Avolio, Andrea Calcagno
Summary: This study aimed to evaluate the role of genetic polymorphisms in affecting COVID-19-related clinical features and found different genetic variants that may affect symptoms and oxygenation. Furthermore, sub-analyses considering gender and age showed a different impact of polymorphisms based on these characteristics. This is the first study highlighting the possible contribution of genetic variants in these pathways to COVID-19 clinical features.
Article
Biochemistry & Molecular Biology
Jessica Cusato, Alessandra Manca, Alice Palermiti, Jacopo Mula, Martina Costanzo, Miriam Antonucci, Mattia Trunfio, Silvia Corcione, Francesco Chiara, Elisa Delia De Vivo, Alice Ianniello, Micol Ferrara, Giovanni Di Perri, Francesco Giuseppe De Rosa, Antonio D'Avolio, Andrea Calcagno
Summary: This study aimed to quantify biomarkers involved in the MAPK pathway and clarify its possible role in affecting COVID-19-related clinical features. The results showed that MAPK-related biomarker levels were higher in SARS-CoV-2-positive participants compared to negative ones, and dyspnea was associated with PBMC C-RAF levels and ventilation type in positive participants. This may help identify COVID-19 positive participants at risk of serious complications.
Article
Chemistry, Medicinal
Alessandra Manca, Amedeo De Nicolo, Elisa Delia De Vivo, Micol Ferrara, Sharon Oh, Sahar Khalili, Niamh Higgins, Robert G. Deiss, Stefano Bonora, Jessica Cusato, Alice Palermiti, Jacopo Mula, Sara Gianella, Antonio D'Avolio
Summary: This study developed an ultra-high-performance liquid chromatography coupled with tandem mass spectrometry method for the simultaneous quantification of opioids in various tissues. Higher concentrations of opioids were observed in tissues compared to plasma, with kidney and liver showing the highest concentrations. The method proved to be suitable for pharmacological/toxicological studies using post-mortem specimens.
Article
Chemistry, Analytical
Simone Cavalera, Thea Serra, Antonio Abad-Fuentes, Josep V. Mercader, Antonio Abad-Somovilla, Fabio Di Nardo, Antonio D'Avolio, Amedeo De Nicolo, Valentina Testa, Matteo Chiarello, Claudio Baggiani, Laura Anfossi
Summary: Highly active antiretroviral therapy (HAART) can have toxic effects due to potent drugs like Tenofovir (TFV). Successful treatment relies on proper management of TFV, and therapeutic drug monitoring (TDM) of TFV concentrations in saliva can be a valuable tool for determining treatment compliance. We have developed a highly sensitive ELISA and LFIA for quantification and screening of TFV in saliva, respectively.
Editorial Material
Biology
Silvia De Francia, Francesco Chiara, Sarah Allegra
Editorial Material
Hematology
Massimo Breccia, Giuseppe Saglio
Summary: The role of IFN alpha in chronic myeloid leukaemia patients who have achieved a deep molecular response is still unclear. The study by Irani et al., which examines the effects of the drug in combination with a second-generation tyrosine kinase inhibitor, provides a foundation for further clinical studies aiming to increase the rate of DMR and potentially successful discontinuation.
BRITISH JOURNAL OF HAEMATOLOGY
(2023)
Article
Infectious Diseases
Silvia Corcione, Davide Vita, Amedeo De Nicolo, Silvia Scabini, Simone Mornese Pinna, Jessica Cusato, Mauro Mangiapia, Antonio D'Avolio, Francesco Giuseppe De Rosa
JOURNAL OF ANTIMICROBIAL CHEMOTHERAPY
(2023)
Article
Biochemistry & Molecular Biology
Sarah Allegra, Emma Dondi, Francesco Chiara, Silvia De Francia
Summary: This study examined the influence of age and sex on the levels of tyrosine kinase inhibitors in plasma and cerebrospinal fluid of patients with chronic myeloid leukaemia. Results showed an inverse correlation between age and imatinib plasma concentration, and lower dasatinib concentrations in plasma compared to cerebrospinal fluid, especially in paediatric patients. Therapeutic drug monitoring, along with tyrosine kinase inhibitors, is supported for the treatment of chronic myeloid leukaemia. Refinement of the classification of tyrosine kinase inhibitor therapeutic drug monitoring should consider factors like sex and age.
Review
Biochemistry & Molecular Biology
Fulvio Borella, Stefano Fucina, Luca Mangherini, Stefano Cosma, Andrea Roberto Carosso, Jessica Cusato, Paola Cassoni, Luca Bertero, Dionyssios Katsaros, Chiara Benedetto
Summary: Epithelial ovarian cancer (EOC) is a leading cause of cancer-related death in women, and its treatment remains challenging with poor survival rates. While the role of hormone receptors (HRs) in EOC has been actively explored, the use of hormone therapy (HT) in treating these tumors is still not well-established. Available data mostly come from retrospective series and small early clinical trials, suggesting that HT may be beneficial in adjuvant therapy, maintenance therapy, or in cases of recurrent disease, particularly for certain subtypes of EOC.
Article
Nutrition & Dietetics
Amedeo De Nicolo, Jessica Cusato, Cristina Bezzio, Simone Saibeni, Marta Vernero, Michela Disabato, Gian Paolo Caviglia, Alice Ianniello, Alessandra Manca, Antonio D'Avolio, Davide Giuseppe Ribaldone
Summary: This study found that VD supplementation, therapy with monoclonal antibodies, and the use of mesalazine may independently predict SARS-CoV-2 positivity in IBD patients. Hypertension was associated with severe disease, while a VD level higher than 30 ng/mL was associated with asymptomatic infection. These results confirm the importance of VD levels in defining the risk of COVID-19 and give encouraging data about the safety of maintaining immunomodulatory treatments for IBD during the COVID-19 pandemic.
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)