Article
Chemistry, Analytical
Yan Wang, Jing-jing Zhang, Zi-xia Wang, Fang Cui, Qian-Nian Zhang, Ping-ping Song, Bing Li, Zhuo-shi Tang, Fang-di Hu, Xiao-feng Shi
Summary: In this study, we investigated the comprehensive chemical characterization of Codonopsis Radix and its processed products using metabolomics and glycomics. The results showed significant chemical changes in the processed Codonopsis Radix, including color and odor. By analyzing differential compounds and chemical mechanisms, we provided a chemical basis for the application of Codonopsis Radix decoction pieces of various types.
JOURNAL OF SEPARATION SCIENCE
(2022)
Review
Plant Sciences
Minmin Li, Huajuan Jiang, Yule Hao, Kequn Du, Hongling Du, Chuan Ma, He Tu, Yao He
Summary: Radix Rehmanniae (RR) is a traditional Chinese herbal medicine with clinical value and rich resources. It has multiple pharmacological actions and can be used to treat various diseases. RR can be processed into two different products, which have significant changes in properties, taste, efficacy, clinical application, chemical composition, and pharmacological action.
JOURNAL OF ETHNOPHARMACOLOGY
(2022)
Article
Plant Sciences
Hui Zhu, Yating Zhang, Yu Duan, Ke Pei, Sicong Tu, Yijing Chen, Hao Cai
Summary: This study investigates the effects of vinegar-processing and compatibility on the pharmacokinetics of active compounds in Sinisan, a classical formula for the treatment of depression. The results show that vinegar-processing and compatibility can influence the pharmacokinetic profiles of active compounds in Sinisan and may be related to the regulation of hepatic drug metabolizing enzymes.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Yanyan Qian, Li Wang, Zhiqiang Zhang, Xinxin Li, Chaofei Niu, Xiao Li, Erjuan Ning, Bingji Ma
Summary: In this study, crude heteropolysaccharides were extracted from four forms of Rehmannia glutinosa, and their physicochemical properties, blood-enriching and immunomodulatory activities were analyzed. The results showed that these heteropolysaccharides had good thermal stability and could improve blood enrichment and immune function, as well as stimulate mRNA levels of tumor necrosis factor and interleukins. Furthermore, different heteropolysaccharides from R. glutinosa exhibited variations in immunomodulatory activities, providing experimental evidence for their potential applications in pharmaceuticals and functional foods.
PROCESS BIOCHEMISTRY
(2022)
Article
Chemistry, Analytical
Ming Zhou, Wei Zheng, Xinguang Sun, Ming Yuan, Jie Zhang, Xiaojuan Chen, Kate Yu, Baolin Guo, Baiping Ma
Summary: This study used ultra high-performance liquid chromatography and multivariate statistical analysis to analyze the chemical components of different parts of Epimedium herb from three species, and identified potential chemical markers for differentiation. The results showed clear differences in the distribution of chemical components with high content in leaves, stems, and rhizomes, providing a foundation for accurately distinguishing different plant parts of Epimedium herb.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Spectroscopy
Tian Jia-yi, Ma Fang, Han Ling-yu
Summary: This study used ATR-FTIR and ion chromatography to investigate the differences in chemical constituents among fresh Radix Rehmanniae (RR), crude RR, and processed RR. The results showed that polysaccharides in fresh RR were hydrolyzed into oligosaccharides or monosaccharides during processing, and the positions and intensities of characteristic peaks of saccharides changed. Quantitative analysis revealed that the contents of different monosaccharides and oligosaccharides varied among the three types of RR extracts.
SPECTROSCOPY AND SPECTRAL ANALYSIS
(2022)
Article
Integrative & Complementary Medicine
Gu Min, Yuan Yi-Ping, Qin Zi-Nan, Xu Yan, Shi Nan-Nan, Wang Yan-Ping, Zhai Hua-Qiang, Qian Zhong-Zhi
Summary: This study aimed to establish a comprehensive method for the quality evaluation and control of Raw Rehmanniae Radix (RRR) pieces based on their chemical constituents, appearance traits, and origins. Through quantitative analysis of the chemical constituents and appearance traits of RRR pieces from different provinces, as well as utilizing correlation analysis, principal components analysis, cluster analysis, and t-test, the study showed that the combined method could effectively distinguish RRR pieces with different qualities, providing a basic reference for the quality control of Chinese herbal medicine.
CHINESE JOURNAL OF NATURAL MEDICINES
(2021)
Article
Chemistry, Multidisciplinary
Dewei Luo, Mingzhen He, Junmao Li, Hui Du, Qiping Mao, Na Pei, Guoyue Zhong, Hui Ouyang, Shiling Yang, Yulin Feng
Summary: This study established a method for evaluating the chemical composition and metabolic differences between Chinese official rhubarb (COR) and Tibetan rhubarb (TR). Significant chemical differences were found between COR and TR, providing valuable insights for the development of their varieties and clinical applications.
ARABIAN JOURNAL OF CHEMISTRY
(2021)
Article
Microbiology
Yuping Chen, Siyuan Song, Anmei Shu, Liping Liu, Jinjin Jiang, Ming Jiang, Qin Wu, Huiqin Xu, Jihu Sun
Summary: Research shows that the Traditional Chinese Medicine pair Radix Rehmanniae and Cornus Officinalis can protect against diabetes-induced testicular damage by modulating gut microbiota. This protection may be mediated by the butyric acid/GLP/GLP-1R pathway.
FRONTIERS IN MICROBIOLOGY
(2022)
Article
Integrative & Complementary Medicine
Shujuan Xue, Yu Fu, Xiaoya Sun, Suiqing Chen
Summary: This study investigated the chemical compositions of distillates obtained from different processing cycles of processed Rehmanniae Radix. Variations in the composition of the distillates were observed. By using GC-MS and LC-MS, a total of 13 and 21 compounds were identified, including organic acids, furans, alcohols, iridoid glycosides, phenylpropanoid glycosides, and saccharides. The content of compound 2,5-hydroxymethylfurfural and furans increased gradually with steaming time, while other compounds exhibited a negative trend or fluctuation. Iridoid glycosides and phenylpropanoid glycosides, which were unstable and easily degraded, showed a decreasing concentration with increased steaming times. These findings provide important references for the processing methods, quality evaluation, and rational clinical application of processed Rehmanniae Radix and its distillate.
EVIDENCE-BASED COMPLEMENTARY AND ALTERNATIVE MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Lele Li, Zhixia Chang, Keyu Wei, Yi Tang, Zhao Chen, Hongli Zhang, Yang Wang, Heyun Zhu, Bo Feng
Summary: This study proposed a new method for discriminating and quantifying black ginseng. The black ginseng samples were divided into incomplete and complete black ginseng based on accurate quantification of six ginsenosides. Ultrahigh-performance liquid chromatography-quadrupole-time of flight/mass spectrometry (UPLC-Q-TOF/MS) combined with a multivariate statistical analysis strategy was then employed to differentiate the two groups.
Article
Food Science & Technology
Lan Yang, Rongrong Li, Xuemei Qin, Zhenyu Li
Summary: This study compared cultivated Astragali Radix (AR-C) and wild/semi-wild Astragali Radix (AR-W) using ultra-high-performance liquid chromatography coupled with hybrid triple quadrupole time-of-flight mass spectrometry. Chemical investigation of Astragali Radix led to the tentative annotation of 196 compounds, and further analysis showed that 55 compounds had higher contents in the wild/semi-wild Astragali Radix. Thirteen compounds showed FC values higher than 3, and one of them could be used as a marker for discrimination of AR-C and AR-W. The study also found differences in the substitution of certain compounds between the two types of Astragali Radix.
EUROPEAN FOOD RESEARCH AND TECHNOLOGY
(2022)
Article
Chemistry, Applied
Liuwei Zhao, Caijun Ren, Xiaofeng Xue, Huanxian Lu, Kai Wang, Liming Wu
Summary: This study characterized monofloral safflower honey (MSH) from a metabolomic perspective and proposed safflomin A as a reliable chemical marker for its authentication. Commercial non-safflower honeys did not test positive for safflomin A, indicating its potential as a fingerprinting chemical marker for safflower honey authentication.
Article
Chemistry, Applied
Xiaokang Liu, Yunlong Guo, Guangzhi Cai, Jiyu Gong, Yang Wang, Shuying Liu
Summary: An UHPLC-Q-Orbitrap/MS-based metabolomics method was used to study the chemical diversity of Schisandra chinensis fructus and its processed products. The study found clear classification among the four Schisandra products and identified 28 marker compounds. These results are important for the quality evaluation of Schisandra products.
NATURAL PRODUCT RESEARCH
(2022)
Article
Plant Sciences
Lung-Shuo Wang, Po-Jen Chen, Wen-Chi Cheng, Yu-Chia Chang, Mohamed El-Shazly, Lo-Yun Chen, Bo-Rong Peng, Chun-Han Su, Pei-Tzu Yen, Tsong-Long Hwang, Kuei-Hung Lai
Summary: This study applied the multi-informative molecular networking (MIMN) approach to construct the anti-inflammatory metabolomic pattern of Scrophularia ningpoensis Hemsl. The compounds characterized by cinnamic acid glycosides showed higher bioactivity and were confirmed as chemical markers for S. ningpoensis. The correct selection of markers will contribute to the precise analysis and classification of medicinal plants.
FRONTIERS IN PLANT SCIENCE
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)