Liquid chromatography/tandem mass spectrometry study of anti-inflammatory activity of Plantain (Plantago L.) species

To evaluate anti-inflammatory activity of selected Plantago species (P lanceolata L and P major L.) an optimized in vitro test for deter mination of cyclooxygenase-1 (COX-1) and 12-lipoxygenase (12-LOX) inhibition potency was undertaken By using intact cell system (platelets) as a source of COX-1 and 12-LOX enzymes and highly sensitive and specific LC-MS/MS technique for detection of main arachidonic acid metabolites formed by COX-I and 12-LOX, this test provides efficient method for evaluation of anti-inflammatory potential of plant extracts and isolated compounds Our results validated the well-known COX-1 inhibitor y activity of P lanceolata and P major methanol extracts (concentration required for 50% inhibition (IC(50)) was 2 00 and 065 mg/ml. respectively). Furthermore, 12-LOX inhibitory activity of examined extracts was reported for the first time (IC(50) = 0 75 and 1.73 mg/ml for P. lanceolata and P major, respectively) Although renowned inhibitor s. such as acetylsalicylic acid and quercetin showed higher activity, this study verifies P lanceolata and P major as considerable anti-inflammatory agents. (C) 2010 Elsevier B V All rights reserved