Article
Biochemistry & Molecular Biology
Luke B. Allen, Karoly Mirnics
Summary: De novo sterol synthesis plays a critical role in maintaining brain homeostasis. However, certain medications, including beta-blockers, can inhibit this process and potentially harm brain health. This study found that two commonly used beta-blockers, metoprolol and nebivolol, significantly increased the production of the highly oxidizable cholesterol precursor 7-dehydrocholesterol in vitro. Follow-up experiments in mice also showed elevated levels of 7-dehydrocholesterol in the brain and other organs of the offspring when the mother was exposed to metoprolol during pregnancy. These findings suggest that the use of metoprolol during pregnancy may have detrimental effects on the development of offspring, and alternative beta-blockers should be considered.
Article
Biochemical Research Methods
Yu Zhang, Jian He, Xiao Tang, Zunjian Zhang, Yuan Tian
Summary: A highly sensitive LC-MS/MS method was developed and applied to simultaneously detect SV and SVA in beagle plasma for an absolute bioavailability study. The method demonstrated good accuracy, precision, extraction recovery, matrix effect, and stability. The absolute bioavailability of SV and SVA in beagles were 2.97% and 25.40%, respectively.
BIOMEDICAL CHROMATOGRAPHY
(2022)
Article
Biochemistry & Molecular Biology
Alessandro Di Giorgi, Giorgia Sprega, Lourdes Poyatos, Esther Papaseit, Clara Perez-Mana, Annagiulia Di Trana, Maria Rosaria Vari, Francesco Paolo Busardo, Simona Pichini, Simona Zaami, Alfredo Fabrizio Lo Faro, Magi Farre
Summary: The aim of this study was to determine the excretion of methylone and its metabolites in sweat after controlled doses of 50, 100, 150, and 200 mg of methylone were ingested by twelve healthy volunteers in a clinical trial. Methylone and its metabolites, 4-hydroxy-3-methoxy-N-methylcathinone (HMMC) and 3,4-methylenedioxycathinone (MDC), were detected in sweat patches using liquid chromatography-tandem mass spectrometry. Methylone and MDC were found in sweat after 2 hours and reached their highest concentration at 24 hours following the administration of doses. However, HMMC was not detectable at any time interval. Sweat has proven to be a suitable matrix for determining the concentration of methylone and its metabolites in clinical and toxicological studies, indicating recent drug consumption.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Lourdes Poyatos, Alfredo Fabrizio Lo Faro, Diletta Berardinelli, Giorgia Sprega, Sara Malaca, Simona Pichini, Marilyn A. Huestis, Esther Papaseit, Clara Perez-Mana, Francesco Paolo Busardo, Magi Farre
Summary: This study defined the pharmacokinetics of methylone and HMMC in human plasma for the first time and validated a new LC-MS/MS method. Methylone exhibited dose-proportional pharmacokinetics, while HMMC showed faster kinetics compared to methylone.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Chemistry, Physical
Agata Kowalczyk, Bozena Zgardzinska, Karol Osipiuk, Katarzyna Jedruchniewicz, Katarzyna Tyszczuk-Rotko, Magdalena Gozdziuk, Haitao Wang, Bozena Czech
Summary: Water is essential for life on Earth, but the increasing global population has led to higher water consumption and increased production of wastewater. Current wastewater treatment techniques are not efficient enough to remove pharmaceuticals, highlighting the need for innovative approaches. This study investigates the photocatalytic oxidation of beta-blockers using modified TiO2 photocatalysts. The results show that solely relying on pollutant concentration reduction is insufficient for determining treatment method safety and additional ecotoxicity testing is necessary.
Article
Chemistry, Analytical
Yanfei Chen, Zhaoliang Hu, Wenzhu Qi, Shuxiao Gao, Jing Jiang, Shixiao Wang, Lei Xu, Xin Xu, Min Song, Taijun Hang
Summary: The study demonstrates that there are interactions between Chinese herbal drugs and chemotherapy drugs, and inappropriate combinations should be avoided to prevent potential adverse drug reactions.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2021)
Article
Biochemistry & Molecular Biology
Xin Shen, Gaofu Li, Libin Wang, Huijin Yu, Lei Zhou, Huifang Deng, Ningning Wang, Chengcai Lai, Wei Zhou, Yue Gao
Summary: This study evaluated the pharmacokinetics of acipimox in rats under simulated high altitude hypoxia conditions. A sensitive LC-MS/MS method was established for quantification of acipimox in rat plasma and tissue homogenate. The results showed significant differences in the pharmacokinetics parameters of acipimox between normoxic and hypoxic rats. Changes in lipid metabolism-related proteins and free fatty acid levels were also observed.
Article
Chemistry, Multidisciplinary
Xucong Huang, Zhenghua Wu, Yanchao Liu, Mengqi Jia, Wenjuan Zhao, Shuowen Wang, Xinhui Jiang, Yuefen Lou, Guorong Fan
Summary: A simple, sensitive, and rapid LC-MS/MS method was developed for the simultaneous determination of BIC, M7, and M8 active metabolites in SD rat plasma. The method showed excellent linearity and accuracy for quantitation in plasma samples. Pharmacokinetic analysis revealed improved parameters in the BIC-NPs group compared to the BIC group, with a significant increase in AUC(0-t) for BIC and a double peak phenomenon observed for M7.
ARABIAN JOURNAL OF CHEMISTRY
(2023)
Article
Chemistry, Analytical
Yixin Hu, Jianyuan Wu, Xin Jiang, Guiying Chen, Yang Zhang, Luqin Si, Hongliang Jiang, Jiangeng Huang, Jianying Huang
Summary: A rapid, simple and sensitive LC-MS/MS method was developed and validated to quantify abiraterone and its metabolites in human plasma. The method showed good accuracy, precision and stability, making it suitable for pharmacokinetic studies.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2022)
Article
Chemistry, Analytical
Omnia A. El-Naem, Christine M. El-Maraghy
Summary: This work developed an LC-MS/MS method for the simultaneous analysis of ranitidine and metronidazole in plasma, with a simple protein precipitation procedure for extraction and effective chromatographic separation on a C-18 column. The method showed good sensitivity and accuracy, and could be applied for bioavailability studies.
ANALYTICAL METHODS
(2021)
Article
Biochemical Research Methods
Xiao Tang, Yu Zhang, Yue Yi Zhang, Zunjian Zhang, Yuan Tian
Summary: This study developed an LC-MS/MS method for simultaneous determination of CLP, CLPCA, 2-O-CLP, SV, and SVA in beagle plasma. The results showed that combined administration of SV and CLP affected the metabolic rate of the drugs and their metabolites.
BIOMEDICAL CHROMATOGRAPHY
(2023)
Article
Pharmacology & Pharmacy
Tianming Dai, Weifan Jiang, Min Wang, Zizheng Guo, Renke Dai
Summary: The study utilized a two-period cross-over design to investigate the bioequivalence of gefitinib in dogs. Although the mean values of the two groups were similar, they did not meet the bioequivalence criteria. ANOVA revealed a significant difference between the two periods.
EUROPEAN JOURNAL OF PHARMACEUTICAL SCIENCES
(2021)
Article
Pharmacology & Pharmacy
Linhu Ye, Lei Cheng, Yan Deng, Sen Wang, Xinyu Wu, Shuiping Ou, Qi Chang, Xinqian Zhao, Wen Zhou, Jinghua Yu, Zuqiang Wu
Summary: Licorice (Glycyrrhiza uralensis Fisch) is widely used in China as a food and herbal medication. The present study investigated the pharmacokinetic profile of Glycycoumarin (GCM), a major coumarin in licorice, in rats. The results showed that GCM was rapidly absorbed and transformed into its conjugated metabolites, with low bioavailability and widespread distribution in various tissues except the brain.
FRONTIERS IN PHARMACOLOGY
(2023)
Article
Pharmacology & Pharmacy
Shuning Li, Zhenyao Lu, Li Jiao, Ran Zhang, Yu Hong, Jiye Aa, Guangji Wang
Summary: A surrogate analyte D-4-cystine was used to determine the levels of endogenous cystine in mice, showing the pharmacokinetics of D-4-cystine and the impact of oral and injected D-4-cystine on endogenous cystine levels. The study found that the absolute bioavailability of cystine increased with dose, with different distribution patterns observed between intravenous and intragastric administration of D-4-cystine.
JOURNAL OF PHARMACEUTICAL ANALYSIS
(2021)
Article
Food Science & Technology
Xiaoyang Ma, Lianzhong Luo, Emad Karrar, Lingyu Zhang, Jian Li
Summary: This study investigates the differences in the digestion, absorption, and metabolism of lignans and their glucosides. The results show that pinoresinol has a faster absorption rate and higher conversion to active metabolites compared to its monoglycoside, pinoresinol-4-O-beta-D-glucopyranoside.
MOLECULAR NUTRITION & FOOD RESEARCH
(2023)
Article
Plant Sciences
Senthamil Selvan Perumal, Sanmuga Priya Ekambaram, T. Dhanam
PHARMACEUTICAL BIOLOGY
(2017)
Review
Chemistry, Medicinal
Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Ajay Balakrishnan
PHYTOTHERAPY RESEARCH
(2016)
Article
Biotechnology & Applied Microbiology
Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Umayal Annamalai
Review
Chemistry, Multidisciplinary
E. Sanmuga Priya, P. Senthamil Selvan
ARABIAN JOURNAL OF CHEMISTRY
(2017)
Article
Immunology
Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Selvaranjani Pavadai
INFLAMMOPHARMACOLOGY
(2017)
Article
Medicine, Legal
Sanmuga Priya Ekambaram, Keerthi B. Babu, Senthamil Selvan Perumal, Durgalakshmi Rajendran
REGULATORY TOXICOLOGY AND PHARMACOLOGY
(2018)
Article
Chemistry, Multidisciplinary
Perumal Moorthy, Sanmuga Priya Ekambaram, Senthamil Selvan Perumal
ARABIAN JOURNAL OF CHEMISTRY
(2019)
Article
Immunology
Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Thamizharasi Erusappan, Aswini Srinivasan
INFLAMMOPHARMACOLOGY
(2020)
Article
Biochemistry & Molecular Biology
Aswini Srinivasan, Sanmuga Priya Ekambaram, Senthamil Selvan Perumal, Jenifer Aruldhas, Thamizharasi Erusappan
JOURNAL OF FOOD BIOCHEMISTRY
(2020)
Article
Biochemistry & Molecular Biology
Aswini Srinivasan, Jenifer Aruldhas, Senthamil Selvan Perumal, Sanmuga Priya Ekambaram
Summary: The study showed that PCA-AX-L and PCA-AX-K extracted from millet seeds have different immunomodulatory effects, with PCA-AX-L acting as an immune stimulant and PCA-AX-K as an immunomodulator. By activating the ERK and NF-kappa B signaling pathways, PCA-AX-L regulates the immune response in RAW 264.7 cells.
JOURNAL OF FOOD BIOCHEMISTRY
(2021)
Review
Cell Biology
P. Sivasakthi, E. Sanmuga Priya, P. Senthamil Selvan
Summary: Rheumatoid arthritis is a chronic inflammatory autoimmune disease with unclear etiology that involves multiple immune signaling pathways and cytokine imbalance. Phytochemicals hold potential in treating RA and may overcome the limitations of conventional therapies.
INFLAMMATION RESEARCH
(2021)
Article
Biochemistry & Molecular Biology
Premnath Sakthivel, Indrajith Sakthivel, Sivasakthi Paramasivam, Senthamil Selvan Perumal, Sanmuga Priya Ekambaram
Summary: Rheumatoid arthritis (RA) is a severe inflammatory disease that causes joint swelling, stiffness and pain, posing a significant global threat. Damage-associated molecular patterns (DAMPs) are endogenous danger molecules that are released during cell injury or death, activating various inflammatory diseases. EDA-fibronectin (Fn) is a DAMP molecule responsible for triggering RA through its interaction with TLR4. This study aimed to reveal the interaction of other PRRs with EDA-Fn in RA through computational methods and identified TLR2, TLR5, and RAGE as potential PRRs.
APPLIED BIOCHEMISTRY AND BIOTECHNOLOGY
(2023)
Article
Immunology
S. Vigneshwaran, K. Maharani, P. Sivasakthi, P. Senthamil Selvan, S. D. Saraswathy, E. Sanmuga Priya
Summary: The study found that Tragia involucrata has potential anti-inflammatory effects and can reduce symptoms of arthritis and restore normal tissue structure. Further analysis revealed the presence of flavonoids such as gallic acid, rutin, and quercetin in the plant, which showed potent anti-inflammatory potential. These findings suggest that Tragia involucrata could be a potential therapeutic candidate for treating inflammatory diseases.
INFLAMMOPHARMACOLOGY
(2023)
Article
Plant Sciences
Sivasakthi Paramasivam, Senthamil Selvan Perumal
Summary: The anti-osteoporotic effect of Methanolic extract of Oldenlandia umbellata (MOU) was evaluated in MG-63 cells and RANKL-stimulated RAW 264.7 cells. MOU promoted osteoblast differentiation and inhibited osteoclast formation through regulating multiple signaling pathways. These findings highlight the potential of MOU as a therapeutic agent for the treatment of osteoporosis.
JOURNAL OF ETHNOPHARMACOLOGY
(2023)
Article
Chemistry, Medicinal
Sivasakthi Paramasivam, Senthamil Selvan Perumal
Summary: This study computationally screened the phytochemical constituents of Oldenlandia umbellata Linn against the therapeutic targets of osteoporosis and validated the results using molecular dynamics simulation. The results showed that deacetylasperuloside, a compound found in Oldenlandia umbellata Linn, exhibited good drug-likeness and bioactive scores and could potentially bind with the molecular targets of osteoporosis. Molecular dynamic simulation further confirmed the stability of the ligand-protein complexes involving deacetylasperuloside.
PHARMACOGNOSY MAGAZINE
(2023)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)