Review
Biochemistry & Molecular Biology
Robert J. J. Asaro, Elisabetta Profumo, Brigitta Buttari, Pedro Cabrales
Summary: The presence of red blood cells (RBCs) in the vasculature and their reactivity have sparked discussions about their role(s) in health and disease. These roles are linked to the development of adhesiveness and eventual clearance by macrophages in the spleen. This review explores the mechanisms involved and provides novel perspectives for identifying RBC adhesiveness.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Chemistry, Physical
Haitao Wang, Dongdong Zhang, Yiping Zhao, Manman Xie
Summary: This study aimed to improve the adsorption and removal function of attapulgite (ATP) for phenol by synthesizing a cationic surfactant Gemini and modifying ATP. The modified ATP showed better dispersibility and abundant active cationic groups on the surface. The positively charged modified ATP with a hydrophobic surface was able to interact with the phenol molecules through 7C-7C interaction and electrostatic attraction. The adsorption behavior followed the Freundlich isotherm model and the modified ATP showed good reusability.
COLLOIDS AND SURFACES A-PHYSICOCHEMICAL AND ENGINEERING ASPECTS
(2022)
Article
Polymer Science
Annelore Aerts, Sean J. D. Lugger, Johan P. A. Heuts, Rint P. Sijbesma
Summary: A new mechanophore for polyurethane thermoplastic elastomers based on ion-paired complexes is developed, where the incorporation of HEPTS leads to changes in fluorescence emission color. Stretching the solid polymer results in dissociation of HEPTS aggregates and a shift in fluorescence emission color.
MACROMOLECULAR RAPID COMMUNICATIONS
(2021)
Article
Biology
Prabuddho Mukherjee, Kamendra Kumar, Bincy Babu, Jubilee Purkayastha, Sudhir Chandna
Summary: This study identifies alterations in expression levels of RBC membrane associated proteins following gamma-irradiation in rats, suggesting their potential as radiation exposure biomarkers. Through two-dimensional electrophoresis analysis, several differentially expressed proteins were identified, with peroxiredoxin-2 (PRDX2) and 26S proteasome regulatory subunit RPN11 (PSMD14) showing the most significant over-expression. These findings provide important insights for further research on using RBC membrane associated proteins as radiation biomarkers.
INTERNATIONAL JOURNAL OF RADIATION BIOLOGY
(2023)
Article
Biochemical Research Methods
Madhumita, Sushmita Paul
Summary: Identification of cancer subtypes is crucial for understanding tumor heterogeneity. This study proposes a novel method that combines feature weighting and similarity network fusion to identify cancer subtypes. The method is applied to three different cancer datasets and compared with existing methods, demonstrating its effectiveness.
IEEE-ACM TRANSACTIONS ON COMPUTATIONAL BIOLOGY AND BIOINFORMATICS
(2022)
Review
Physiology
Sebastian Himbert, Maikel C. Rheinstadter
Summary: Red blood cells, the most abundant cell type in the human body, have a simple structure and are both flexible and resistant to shear stress. The composition and organization of their outer shell play a crucial role in their mechanical properties. Current research indicates that the structure of the cell membrane is patchy, with liquid ordered and disordered lipid and peptide domains. Contrary to previous beliefs, the membrane is surprisingly soft, potentially due to the interaction between polyunsaturated lipids and cholesterol.
FRONTIERS IN PHYSIOLOGY
(2022)
Article
Biochemical Research Methods
Christina Vanhinsbergh, Elliot C. Hook, Nicola Oxby, Mark J. Dickman
Summary: In this study, a method for analyzing closely related oligonucleotides and oligonucleotide manufacturing impurities was developed by optimizing different 1D-LC methods and coupling two orthogonal chromatographic modes in a 2D-LC workflow. The method resulted in an increased orthogonality and a reduction in co-elution, and the application of a UV based reference mapping strategy reduced the analytical complexity and reliance on mass-based detection methods.
JOURNAL OF CHROMATOGRAPHY B-ANALYTICAL TECHNOLOGIES IN THE BIOMEDICAL AND LIFE SCIENCES
(2023)
Article
Biology
Stephanie W. Tam, Kate Huffer, Mufeng Li, Kenton J. Swartz
Summary: P2X receptor channels are trimeric ATP-activated ion channels expressed in neuronal and non-neuronal cells. Seven subtypes have been identified in mammals that can form both homomeric and heteromeric channels. The recent study demonstrates that ions can enter or exit the internal pore through lateral fenestrations, which play a critical role in determining the ion selectivity of P2X receptor channels.
Article
Biochemistry & Molecular Biology
Abhinav Parashar, Vivian David Jacob, Daniel Andrew Gideon, Kelath Murali Manoj
Summary: The high ATP concentration in erythrocytes may be catalyzed by hemoglobin serving as a ‘murzyme’, explaining discrepancies in previous findings. Adenosine nucleotide and 2,3-bisphosphoglycerate binding sites on Hb allow interaction with superoxide, potentially providing a new mechanism for ATP synthesis. The new hypothesis for Hb binding affinities and lower affinity for 2,3-BPG in fetal Hb compared to adults may have significant implications in physiology and pathologies like sickle cell anemia and thalassemia.
JOURNAL OF BIOMOLECULAR STRUCTURE & DYNAMICS
(2022)
Article
Biotechnology & Applied Microbiology
Swe Soe Maung Ye, Sangho Kim
Summary: Red blood cell aggregation occurs under low flow conditions, and plasma-mediated aggregation involves cross-bridging mechanisms. Fibrinogen plays a dominant role in promoting aggregate formation, and specific and non-specific cross-bridging have different effects on aggregation and disaggregation.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Leonid Livshits, Gregory Barshtein, Dan Arbell, Alexander Gural, Carina Levin, Helene Guizouarn
Summary: Red blood cell (RBC) transfusion is common in medicine but can have negative side effects. Cell quality is crucial for transfusion effectiveness and safety. Research suggests that high sugar concentration may damage red blood cells.
Article
Cell Biology
Zhitong Ge, Penghui Feng, Zijuan Zhang, Jianchu Li, Qi Yu
Summary: This study identified differential metabolites between IVL patients and healthy controls, with hypoxanthine and glycerophosphocholine potentially serving as protective factors, while acetylcarnitine and hydrocortisone as risk indicators for IVL development. These findings suggest a potential role of these hub metabolites as novel biomarkers for predicting the prognosis or progression of IVL.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Wuan-Geok Saw, Khoa Cong Minh Le, Joon Shin, Jes Hui Min Kwek, Chui Fann Wong, Priya Ragunathan, Tuck Choy Fong, Volker Mueller, Gerhard Grueber
Summary: This study investigates the self-inhibition mechanism of ATP hydrolysis in A. baumannii F-1-ATPase (AbF(1)-ATPase) by generating and purifying a recombinant AbF(1)-ATPase and analyzing its structure. The results reveal that the C-terminal domain of subunit epsilon (Ab epsilon) plays a major role in regulating ATP hydrolysis in AbF(1)-ATPase. The findings have important implications for understanding the regulation of ATP hydrolysis.
Review
Pharmacology & Pharmacy
Francesco Di Virgilio, Valentina Vultaggio-Poma, Alba Clara Sarti
Summary: Ion channels, especially P2X receptors, play crucial roles in tumor development, closely related to extracellular ATP, and could be key regulators of tumor growth.
BIOCHEMICAL PHARMACOLOGY
(2021)
Review
Physiology
Gregory Barshtein, Ivana Pajic-Lijakovic, Alexander Gural
Summary: Red blood cells' deformability plays a crucial role in adapting to flow conditions, with high deformability facilitating flow through small blood vessels. Loss of deformability may lead to cell rigidity and dysfunction.
FRONTIERS IN PHYSIOLOGY
(2021)
Article
Pharmacology & Pharmacy
Remigius U. Agu, Jared Mactavish, Pollen K. Yeung, Syed Ali Imran
EXPERT OPINION ON DRUG DELIVERY
(2016)
Article
Biochemistry & Molecular Biology
Jordan J. Bartlett, Purvi C. Trivedi, Pollen Yeung, Petra C. Kienesberger, Thomas Pulinilkunnil
BIOCHEMICAL JOURNAL
(2016)
Article
Pharmacology & Pharmacy
Michelle Amoako-Tuffour, Pollen K. Yeung, Remigius U. Agu
ACTA PHARMACEUTICA
(2009)
Article
Pharmacology & Pharmacy
Tiam Feridooni, Chris Mac Donald, Di Shao, Pollen Yeung, Remigius U. Agu
ACTA PHARMACEUTICA
(2013)
Article
Biotechnology & Applied Microbiology
Lauren C. Klein, Pollen K. Yeung, Jason N. Berman
Article
Medicine, Research & Experimental
Yixian Li, Juan Zhou, Ian Burkovskiy, Pollen Yeung, Christian Lehmann
MEDICAL HYPOTHESES
(2019)
Article
Pharmacology & Pharmacy
Ayah Shakshuki, Pollen Yeung, Remigius U. Agu
JOURNAL OF THE AMERICAN PHARMACISTS ASSOCIATION
(2019)
Review
Biochemistry & Molecular Biology
Pollen K. Yeung, Shyam Sundar Kolathuru, Sheyda Mohammadizadeh, Fatemeh Akhoundi, Brett Linderfield
Article
Biochemistry & Molecular Biology
Pollen K. F. Yeung, Zhaolin Xu, Dena Seeto
Article
Pharmacology & Pharmacy
Pollen K. E. Yeung, Angelita Alcos, Jinglan Tang, Ban Tsui
BIOPHARMACEUTICS & DRUG DISPOSITION
(2007)
Article
Pharmacology & Pharmacy
Pollen K. F. Yeung, Joe D. Feng, Debbra Fice
JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
(2006)
Article
Biotechnology & Applied Microbiology
PK Yeung
CURRENT OPINION IN MOLECULAR THERAPEUTICS
(2003)
Article
Pharmacology & Pharmacy
PKF Yeung, A Jamieson, GJ Smith, D Fice, PT Pollak
INTERNATIONAL JOURNAL OF PHARMACEUTICS
(2000)
Article
Pharmacology & Pharmacy
Pollen K. F. Yeung, Carrie Ferguson, Ameer Jarrar, Brian King, Mary L. Li
JOURNAL OF PHARMACY AND PHARMACEUTICAL SCIENCES
(2007)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)