Article
Biochemistry & Molecular Biology
Stefani Fertaki, Georgios Bagourakis, Malvina Orkoula, Christos Kontoyannis
Summary: The study developed a method for measuring the particle size and morphology of active pharmaceutical ingredients (APIs) in film-coated tablets. The results showed that the manufacturing process of the formulation affected the particle size and morphology of the API.
Article
Spectroscopy
Karlis Berzins, Samuel D. L. Harrison, Claudia Leong, Sara J. Fraser-Miller, Michelle J. Harper, Aly Diana, Rosalind S. Gibson, Lisa A. Houghton, Keith C. Gordon
Summary: Raman and ATR-FTIR spectroscopy were used to analyze 208 breast milk samples as part of a larger research study. Comprehensive qualitative and quantitative analysis was carried out using chemometric methods, revealing the relationship between macronutrients and sample metadata. The study location and primary food sources were found to have a stronger impact on fat composition than its absolute content, highlighting the limitations and challenges of using spectroscopic techniques for this type of analysis.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2021)
Article
Chemistry, Analytical
Yingjie Zeng, Zi-quan Liu, Xian-guang Fan, Xin Wang
Summary: Raman spectroscopy is a sensitive and non-destructive technique for structural analysis, but its low signal-to-noise ratio makes it challenging to implement. This paper proposes a MFED model based on a convolutional neural network and multi-scale feature extraction fusion block, which improves the generalization and robustness of the model. The MFED model shows excellent denoising performance using the mixed Poisson-Gaussian noise model and multi-scale feature extraction. The results demonstrate the applicability and superiority of the proposed approach, making it promising for Raman analytics in research and practices.
MICROCHEMICAL JOURNAL
(2023)
Article
Multidisciplinary Sciences
Yiyong Guo, Bing Han, Junting Du, Shanshan Cao, Hua Gao, Ning An, Yiwei Li, Shujie An, Zengling Ran, Yue Lin, Wencai Ren, Yunjiang Rao, Baicheng Yao
Summary: This study demonstrated kilometers long graphene-coated optical fiber (GCF) with unusually high thermal diffusivity, enabling rapid thermooptical response and long-distance sensing systems, providing a novel platform for optical fiber sensing applications.
Article
Chemistry, Applied
Wei-li Wang, Jiang-long Lu, Jia-lei Gu, Li-fang Xie, Jing Chang, Bo Zou, Fang-ling Wang, Guo-kun Liu, Zhong-qun Tian
Summary: The dual functions of aconitine, with biological activity and toxicity, often lead to related food poisoning. Fast and accurate qualitative analysis is crucial for tracking the source of poisoning and administering correct treatments. Through the use of Surface-enhanced Raman spectroscopy (SERS), we have developed a highly sensitive and accurate strategy for the trace detection of three similar aconitines. This strategy shows promise for fast and on-site detection of aconitines in the field of food safety or criminal identification.
Article
Chemistry, Multidisciplinary
Nowzesh Hasan, Urna Kansakar, Eric Sherer, Mark A. DeCoster, Adarsh D. Radadia
Summary: Graphene, with its intrinsic ion sensitivity and sp(2)-hybridized carbon structure, shows promise as a channel material for ion-sensitive field-effect transistors. Coating graphene FETs with ion-selective membranes allows for selective detection of changes in ion concentrations in salt solutions. The addition of a hexagonal boron nitride multilayer between graphene and oxide further enhances ion sensitivity and selectivity, encouraging further exploration in alternative architectures and biomedical applications.
Article
Engineering, Electrical & Electronic
Yanru Bai, Clement Yuen, Quan Liu
Summary: A modified least-squares method is proposed to address challenges in quantitative spectral analysis induced by inaccuracies or variations in the spectral library, updating both the library and analyte concentrations by treating variations as individual Gaussian functions with variable parameters. The method is validated on simulated data with multiple Gaussian peaks and experimental measurements from chemical mixtures and leukemia cells.
IEEE JOURNAL OF SELECTED TOPICS IN QUANTUM ELECTRONICS
(2021)
Article
Chemistry, Medicinal
Motoki Inoue, Takumi Osada, Hiroshi Hisada, Tatsuo Koide, Toshiro Fukami, Anjan Roy, James Carriere
Summary: The study aims to develop a method to monitor cocrystal polymorphs in model tablets using transmission low-frequency Raman spectroscopy. The results show that this technique can be used to quantitatively monitor the physicochemical stability of cocrystal polymorphs.
JOURNAL OF PHARMACEUTICAL SCIENCES
(2023)
Article
Chemistry, Applied
Huanjiong An, Chen Zhai, Fan Zhang, Qianyun Ma, Jianfeng Sun, Yiwei Tang, Wenxiu Wang
Summary: Near-infrared (NIR), mid-infrared (MIR), and Raman spectroscopy combined with data fusion were used for efficient and comprehensive detection of the staling degree of Chinese steamed bread (CSB) stored for 0-16 days. Decision-level fusion achieved the best performance in quantifying the staling degree of CSB. The research has important applications for food quality, safety, and shelf-life evaluations.
Article
Chemistry, Analytical
Tingting Chen, Tianlong Zhang, Chen Niu, Ting Feng, Hongsheng Tang, Xuemei Cheng, Hua Li
Summary: In this study, simultaneous quantitative detection of multiple metallic species in single micro-sized suspended particles was achieved by combining random forest (RF) and variable selection strategies. The proposed method showed better correlation and accuracy compared to traditional techniques, suggesting its potential as a promising tool for real-time air particulate matter pollution monitoring and control in the future.
ANALYTICAL CHEMISTRY
(2022)
Article
Food Science & Technology
Panawan Pruksapha, Putthiporn Khongkaew, Chaweewan Suwanvecho, Nantana Nuchtavorn, Chutima Phechkrajang, Leena Suntornsuk
Summary: In this study, two spectroscopic methods, Fourier transform infrared (FTIR) and Raman spectroscopy, were developed for the simultaneous determination of PQ and DHA in combined tablets. Results showed that these methods could provide similar results to the HPLC-UV method, with the advantages of being fast, economical, and less labor intensive.
JOURNAL OF FOOD AND DRUG ANALYSIS
(2023)
Article
Environmental Sciences
Saurabh Prajapati, Michael Beal, Jason Maley, Markus Brinkmann
Summary: In this study, microplastic and microfiber contamination in the effluents of a wastewater treatment plant in Saskatoon, Canada was qualitatively and quantitatively characterized. Fibers accounted for the majority of synthetic microparticles, with stable concentrations across seasons. Despite relatively low concentrations per liter of effluent, the large daily discharge volume results in significant emissions of particles into the river. Environmental implications of these findings require further investigation, but they represent an important initial step in understanding microplastic contamination in the Canadian Prairies.
ENVIRONMENTAL SCIENCE AND POLLUTION RESEARCH
(2021)
Article
Pharmacology & Pharmacy
Ryo Ohashi, Tatsuo Koide, Toshiro Fukami
Summary: Transmission Raman spectroscopy (TRS) has been found to have practical prediction performance for drug content in tablets, even with variations in granulation parameters. It also shows good prediction performance for tablets manufactured at different granulation scales and thicknesses. Therefore, TRS is a versatile non-destructive quantitative analysis method that can be applied in tablet manufacturing.
EUROPEAN JOURNAL OF PHARMACEUTICS AND BIOPHARMACEUTICS
(2023)
Article
Spectroscopy
Mingdi Liu, Jichao Liu, Qiuhong Wang, Ping Song, Haichao Li, Songgu Wu, Junbo Gong
Summary: This study aimed to quantitatively analyze the low content of CFZ or Mono-CFZ in tablets using PXRD, NIR, ATR-FTIR, and Raman, and establish PLSR calibration models. NIR was found to be the most suitable method for the quantitative analysis of CFZ or Mono-CFZ in tablets.
SPECTROCHIMICA ACTA PART A-MOLECULAR AND BIOMOLECULAR SPECTROSCOPY
(2023)
Article
Cell Biology
Sergey Dubkov, Aleksei Overchenko, Denis Novikov, Vasilii Kolmogorov, Lidiya Volkova, Petr Gorelkin, Alexander Erofeev, Yuri Parkhomenko
Summary: This study presents an original technique for fabricating silver-coated pipettes and explores their use in cell analysis through surface-enhanced Raman spectroscopy and scanning ion-conducting microscopy. The results demonstrate the potential of this method for molecular composition analysis of living cells.
Article
Medicine, Research & Experimental
Harry C. Blair, Elena Kalyvioti, Nicholaos I. Papachristou, Irina L. Tourkova, Spryros A. Syggelos, Despina Deligianni, Malvina G. Orkoula, Christos G. Kontoyannis, Eleni A. Karavia, Kyriakos E. Kypreos, Dionysios J. Papachristou
LABORATORY INVESTIGATION
(2016)
Article
Plant Sciences
Georgia Tooulakou, Andreas Giannopoulos, Dimosthenis Nikolopoulos, Panagiota Bresta, Elissavet Dotsika, Malvina G. Orkoula, Christos G. Kontoyannis, Costas Fasseas, Georgios Liakopoulos, Maria I. Klapa, George Karabourniotis
Article
Food Science & Technology
Eleni Kamilari, Konstantinos Farsalinos, Konstantinos Poulas, Christos G. Kontoyannis, Malvina G. Orkoula
FOOD AND CHEMICAL TOXICOLOGY
(2018)
Article
Food Science & Technology
Zacharoula I. Linardaki, Malvina G. Orkoula, Alexandros G. Kokkosis, Fotini N. Lamari, Marigoula Margarity
FOOD AND CHEMICAL TOXICOLOGY
(2013)
Article
Biochemical Research Methods
Ioannis A. Karampas, Malvina G. Orkoula, Christos G. Kontoyannis
JOURNAL OF BIOPHOTONICS
(2013)
Article
Engineering, Biomedical
I. A. Karampas, M. G. Orkoula, C. G. Kontoyannis
JOURNAL OF MATERIALS SCIENCE-MATERIALS IN MEDICINE
(2012)
Article
Plant Sciences
Georgia Tooulakou, Dimosthenis Nikolopoulos, Elissavet Dotsika, Malvina G. Orkoula, Christos G. Kontoyannis, Georgios Liakopoulos, Maria I. Klapa, George Karabourniotis
PHYSIOLOGIA PLANTARUM
(2019)
Article
Biochemistry & Molecular Biology
Ioanna Chrisikou, Malvina Orkoula, Christos Kontoyannis
Article
Biochemistry & Molecular Biology
Michail Lykouras, Stefani Fertaki, Malvina Orkoula, Christos Kontoyannis
Article
Pharmacology & Pharmacy
Ioanna K. Siakavella, Fotini Lamari, Dimitrios Papoulis, Malvina Orkoula, Patroula Gkolfi, Michail Lykouras, Konstantinos Avgoustakis, Sophia Hatziantoniou
Article
Biochemistry & Molecular Biology
Panagiota Papaspyridakou, Michail Lykouras, Christos Kontoyannis, Malvina Orkoula
Summary: Vibrational spectroscopic techniques, particularly Raman spectroscopy, are gradually replacing chromatographic methods in the identification of ethanol and other volatile substances in body fluids. This study proposes three carriers and describes their corresponding sample preparation methods for determining ethanol in human urine samples. Among the three carriers, the last one achieves a quick, simple, and inexpensive identification of ethanol with prevention of evaporation.
Article
Chemistry, Medicinal
Michail Lykouras, Malvina Orkoula, Christos Kontoyannis
Summary: This study found that the polymorphic conversion of posaconazole from Form I to Form-S, which may affect bioavailability, is due to an interaction with water. However, the poor wettability of posaconazole Form I makes it challenging to completely wet the particles and produce pure Form-S. Therefore, for isolation, Form I should be dispersed in water and subjected to sonication for at least 10 minutes. Pure posaconazole Form-S was characterized as a hydrate form containing three water molecules per API molecule using X-ray powder diffraction (XRPD), Raman spectroscopy, attenuated total reflection (ATR) spectroscopy, thermogravimetric analysis (TGA), and optical microscopy.
Article
Chemistry, Analytical
Panagiota Papaspyridakou, Panagiota Giannoutsou, Malvina G. Orkoula
Summary: The measurement of ethanol and toxic alcohol strengths in beverages and spirits is crucial for health reasons and for identifying adulterated products. Chromatographic and spectroscopic techniques have been used, but their application is time-consuming or requires sample pretreatment. In this study, Raman spectroscopy was used for fast and non-destructive measurements. A through the container method was developed for a non-invasive analysis. The method was validated with calibration curves and used to study the concentrations of alcohol in various products.
Article
Chemistry, Analytical
Panagiota Papaspyridakou, Michail Lykouras, Malvina Orkoula
Summary: “A novel method for the detection and quantification of ethanol and methanol in human urine, saliva, and blood serum using Raman spectroscopy was developed. Calibration curves were created for each alcohol-biological fluid system to determine the detection limits. Methanol had lower detection limits, but were still below intoxication levels, while ethanol had detectable concentrations below permissible limits. Saliva showed the lowest detection limits for both alcohols.”
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2023)
Article
Biochemistry & Molecular Biology
Georgia Tooulakou, Andreas Giannopoulos, Dimosthenis Nikolopoulos, Panagiota Bresta, Elissavet Dotsika, Malvina G. Orkoula, Christos G. Kontoyannis, Costas Fasseas, Georgios Liakopoulos, Maria I. Klapa, George Karabourniotis
PLANT SIGNALING & BEHAVIOR
(2016)
Article
Chemistry, Analytical
Bingbing Liu, Jie Cao, Lu Liu, Min Zeng, Hongli Yu, Hao Wu
Summary: This study investigated the effect of processing with Chebulae Fructus on the chemical composition of Euphorbia pekinensis (EP) and found that it could alter the composition and reduce toxicity, providing a scientific basis for optimizing processing technology and handling other ethnomedicines with toxic properties.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Renuka P. Rathnasekara, Abu M. Rustum
Summary: A simple and robust HPLC method was developed for simultaneous determination of four active pharmaceutical ingredients and their degradation products. The method successfully separated the peaks of interest and demonstrated stability-indicating capability by separating the degradation products in stressed samples.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Bryan Lee, Gita Kharal, Benjamin Sreenan, Claire Lin, Ruosheng Zeng, Colin A. Fox, Patricia Ellison, Robert O. Ryan, Paul J. Brett, David Aucoin, Xiaoshan Zhu
Summary: In this study, a new type of Mn-doped nanocrystals was developed for detecting a biomarker of a fatal disease. The sensitivity of the detection was enhanced by surface treatment and time-resolved reading, achieving a detection limit comparable to enzyme-based immunoassay.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xiaoling Liu, Ting Yang, Lu Chen, Lili Lan, Guoxiang Sun, Ping Guo
Summary: In this study, the quality of Yiqing tablet was comprehensively evaluated using multi-component quantification, multi-dimensional fingerprint construction, and antioxidant activity analysis. The results showed that the sample had 25 components with antioxidant activity according to the HPLC fingerprint and most bands of FTIR also showed antioxidant activity. Based on the evaluation results of HPLC and FTIR fingerprint, all samples were classified as first level, demonstrating the consistency of sample quality. This study provides a new idea for achieving comprehensive quality evaluation of traditional Chinese medicines.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Siqi Li, Hao Guo, Yan Gao, Wen Tian, Shan Wang, Chen Shen, Lili Xu, Hailong Liu, Jinliang Zhang, Yingwu Wang
Summary: Using competitive ELISA to detect free cytokines is limited in accurately determining their real state and quantity. In this study, a novel cytokine immunoassay was developed using Rosetta molecular docking prediction technique to establish a method that can detect the true concentration of free cytokines without disrupting the dynamic equilibrium.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Changqing Yu, Tiantian Xie, Haiyan Liu, Ligai Bai
Summary: A phenyl-hybrid monolithic adsorbent with porous structure and multiple functional groups was prepared. It was used for the online extraction and quantification of coumarins in mouse plasma, showing good removal ability and selectivity.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Joao B. Pereira Jr, Vanessa S. Carvalho, William Q. Ferreira, Rennan G. O. Araujo, Sergio L. C. Ferreira
Summary: This work presents a simple, cost-effective, and environmentally friendly method for determining the content of multiple elements in medicinal herbs using ICP OES. The optimized experimental parameters result in low dissolved organic carbon content and residual acidity concentration. The accuracy of the method is confirmed through the analysis of certified reference materials, and its environmental friendliness is validated using the AGREE metric. Principal component analysis is used to classify the medicinal herbs based on their inorganic constituent concentrations.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Orwa Siddig, Chenxi Liu, Mohamed Abdulbagi, Min Song, Yu-ting Lu, Tai-jun Hang
Summary: In this study, a reliable LC-QTOF-MS method was developed to separate and characterize process-related substances and degradation products of Lurasidone hydrochloride. The results showed that Lurasidone hydrochloride exhibited instability under certain stress conditions, and several previously unidentified compounds were discovered. The study emphasizes the importance of monitoring and controlling related substances in the manufacturing processes to optimize the process and ensure quality control.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Jiaxi Fang, Luping Wang, Di Zhang, Yan Liang, Shouxin Li, Jingkui Tian, Qiang He, Juan Jin, Wei Zhu
Summary: This study reports that a novel compound called CPS-A exhibits therapeutic effects on cisplatin-induced acute kidney injury (AKI) and explores its potential mechanisms through transcriptomics and metabolomics. CPS-A improves cell viability, reduces inflammatory markers, and attenuates cisplatin-induced kidney injury. The study also identifies potential targets related to amino acid metabolism.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ricardo Conde, Nadia Oliveira, Elisabete Morais, Ana Paula Amaral, Adriana Sousa, Goncalo Graca, Ignacio Verde
Summary: Decreased levels of tyrosine, glutamate, valine, leucine, and isoleucine are associated with worsening pathological cognitive decline. Decreased levels of isobutyrate, histidine, acetone, and unknown-1 metabolite are potential biomarkers with neuroprotective and antioxidant actions for cognitive decline.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
A. L. Santunione, F. Palazzoli, P. Verri, D. Vandelli, V. Castagnetti, C. Profeta, E. Silingardi
Summary: This study describes two fatal suicide cases involving four cardiovascular drugs, and presents the concentrations and distribution of these drugs in different post-mortem specimens. The study developed a fast, sensitive, and simple method for drug detection suitable for forensic purposes.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Meiyun Shi, Xinyue Zheng, Yuncheng Ge, Ning Zhang, Luyao Yu, Xujian Duan, Yajun Liu, Hongyu Xue, Jiansong You, Lei Yin
Summary: This study investigated the cellular uptake of low, medium and high molecular weight polyethylene glycol (PEG) in MCF-7 cells using UPLC-MS/MS and CID techniques. The results showed that PEG600, PEG2K, PEG4K and PEG10K exhibited low toxicity and had limited cellular entry at concentrations of 5-1200 μg/mL. Additionally, the environmental friendliness of the developed analytical methods was evaluated.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Ruijin Geng, Mengjie Yu, Jinbiao Xu, Yuanwang Wei, Qiong Wang, Junguo Chen, Fei Sun, Kun Xu, Han Xu, Xiaohui Liu, Juhua Xiao, Xianchao Zhang, Baogang Xie
Summary: This study validated serum methionine as a potential biomarker for Kawasaki disease (KD), and found that its alteration is associated with the activation of DNMT1 in KD patients.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Eman Shawky, Rasha A. Nassra, Aliaa M. T. El-Alkamy, Shaimaa M. Sallam, Samah M. El Sohafy
Summary: This study employed network pharmacology-based analysis and reverse molecular docking to investigate the molecular targets and pathways associated with gynecological disorders and the potential therapeutic effects of fenugreek constituents. The results highlighted the potential of steroidal saponins, isoflavones, and flavones as active constituents of fenugreek with implications for lactation, reproductive processes, and estrogenic activity. Animal model study further supported the presence of these bioactive compounds and their effects on body weight, serum prolactin levels, serum estradiol levels, and ovarian histological structure.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
Article
Chemistry, Analytical
Xuelong Zhao, Ruixue Dai, Jing Wang, Liangliang Cao, Peidong Chen, Weifeng Yao, Fangfang Cheng, Beihua Bao, Li Zhang
Summary: This study identified the skin-permeable and skin-retainable components of Cayratia japonica ointment and found that flavonoids are the main active components that play a crucial role in the inflammatory and proliferative phases of wound healing.
JOURNAL OF PHARMACEUTICAL AND BIOMEDICAL ANALYSIS
(2024)
