Article
Agronomy
Axelle Koreki, Severine Michel, Caroline Lebeaux, Lidwine Trouilh, Christophe Delye
Summary: This study investigates the prevalence, mode of evolution, and spread of resistance-endowing ALS alleles in corn poppy populations in France. With the use of massively parallel amplicon sequencing, it was found that mutant ALS alleles have complex geographic distributions, which could be attributed to large population sizes, multiple independent mutation events, and human-mediated spread of resistance. The study also reveals that mutant ALS alleles are associated with resistance to the synthetic auxin 2,4-D.
PEST MANAGEMENT SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Jia-hui Li, Yan Wang, Yun-peng Wu, Ran-hong Li, Shuang Liang, Jing Zhang, Yong-gang Zhu, Bei-jie Xie
Summary: Based on the pharmacophore binding strategy and the principle of bioelectronic isobaric, a series of novel thiourea compounds containing aromatic-substituted pyrimidines were designed and synthesized using the sulfonylurea bridge as the parent structure. Some compounds showed good herbicidal activity, especially compounds 4d and 4f, which exhibited higher comparative activity than the commercial herbicide bensulfuron-methyl. The preliminary structure-activity relationship (SAR) and in vivo AHAS enzyme activity inhibition experiments further confirmed the activity of these compounds.
PESTICIDE BIOCHEMISTRY AND PHYSIOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Maria Barco-Antonanzas, Miriam Gil-Monreal, Mikel Eceiza, Mercedes Royuela, Ana Zabalza
Summary: This study characterized the resistance mechanisms and metabolism of a multiple resistant population of Amaranthus palmeri to glyphosate and pyrithiobac. The results showed that glyphosate resistance was due to EPSPS gene amplification, while resistance to pyrithiobac was related to point mutations in the ALS gene sequence. The study also found additional effects of EPSPS gene amplification and ALS mutations on the expression of genes in certain metabolic pathways. The physiological response of the resistant population to glyphosate and pyrithiobac was lower compared to the sensitive population.
Article
Agronomy
Yi Cao, Xinxin Zhou, Zhaofeng Huang
Summary: The study revealed the molecular basis for resistance of Amaranthus retroflexus to ALS-inhibiting herbicides, with Trp-574-Leu and Gly-654-Tyr substitutions in ALS contributing to broad-spectrum resistance. The Gly-654-Tyr substitution, previously unreported, conferred high resistance to multiple classes of ALS-inhibiting herbicides in the R-Tyr654 population. Despite this resistance, effective control of the population was achieved with herbicides targeting other sites of action.
PEST MANAGEMENT SCIENCE
(2022)
Article
Plant Sciences
Shmuel Galili, Joseph Hershenhorn, Marvin Edelman, Vladimir Sobolev, Evgeny Smirnov, Orit Amir-Segev, Aharon Bellalou, Evgenia Dor
Summary: Chickpea is an important crop in Israel, but sensitive to imidazolinone herbicides. A chickpea line resistant to these herbicides was developed using chemical mutagenesis, with a point mutation detected in one of the genes encoding the AHAS catalytic subunit. The mutation provided resistance to a specific group of herbicides and showed single-gene semi-dominant inheritance pattern, as confirmed by genetic transformation in tobacco plants.
Article
Plant Sciences
Diego M. Chiapinotto, Luis A. Avila, Carlos E. Schaedler, Sandra M. Mathioni, Claudia Oliveira, Vivian E. Viana, Edinalvo R. Camargo
Summary: This study aimed to identify mutations conferring ALS resistance and the involvement of a metabolic-mediated resistance mechanism in rice flatsedge. The GR(50) values of the resistant population were 400 to >4,000 times higher than those of the susceptible population. The main resistance mechanism was identified as a specific mutation in the ALS gene.
ADVANCES IN WEED SCIENCE
(2023)
Article
Biochemistry & Molecular Biology
Lucie Meyer, Fanny Pernin, Severine Michel, Geraldine Bailly, Bruno Chauvel, Valerie Le Corre, Christophe Delye
Summary: Assessing the capacity of weeds to develop herbicide resistance before it occurs in the field is crucial for effective chemical weed control. This study used herbicide selection and controlled crosses to accelerate the evolution of resistance to two commonly used herbicides in Ambrosia artemisiifolia. The results showed that non-target-site-based resistance (NTSR) to herbicides was significantly increased in just two generations of selection. NTSR to the tested herbicides emerged and was reinforced in subsequent generations. Further bioassays confirmed the emergence of resistance to imazamox and/or tribenuron in multiple A. artemisiifolia populations. NTSR was found to be the predominant type of resistance, with only one population showing a mutant ALS allele in addition to NTSR. This study highlights the importance of pre-emptive assessment of resistance evolution in weed populations.
Article
Biology
Rui Zhang, Sha Chen, Xiangbing Meng, Zhuangzhuang Chai, Delin Wang, Yuge Yuan, Kunling Chen, Linjian Jiang, Jiayang Li, Caixia Gao
Summary: Utilizing genome editing, various missense mutations were created in rice to confer tolerance to different herbicides, with a novel triple mutant showing high tolerance to all tested herbicides. Field trials suggested that these mutated varieties have potential practical applications in weed control.
SCIENCE CHINA-LIFE SCIENCES
(2021)
Article
Biochemical Research Methods
Xian Hui, Jin-Meng Tian, Xin Wang, Zhen-Qian Zhang, Ya-Mei Zhao, Wen-Yun Gao, Heng Li
Summary: A new HPLC method was developed to comprehensively analyze the reactions catalyzed by AHAS/ALS. Compared to the classic Bauerle UV assay, the HPLC method provides a more precise analysis of enzyme characteristics and can measure the formation of products.
ANALYTICAL BIOCHEMISTRY
(2023)
Article
Plant Sciences
Vanessa Vital Silva, Rafael Mendes, Andreia Suzukawa, Fernando Adegas, Francismar Marcelino-Guimaraes, Rubem Oliveira
Summary: The study confirmed cross-resistance of a weed population in Brazil to ALS inhibitors and identified a mutation in the ALS gene. This is the first study in Brazil to identify a target-site change as a survival mechanism in Erigeron sumatrensis for resistance to ALS-inhibiting herbicides.
Article
Plant Sciences
Kashaf Zafar, Muhammad Zuhaib Khan, Imran Amin, Zahid Mukhtar, Mehak Zafar, Shahid Mansoor
Summary: In this study, template-directed CRISPR-Cas9 system was established in Basmati rice, leading to the development of herbicide tolerance by introducing a point mutation in the Acetolactate Synthase gene.
Article
Plant Sciences
Candelario Palma-Bautista, Jose G. Vazquez-Garcia, Maria D. Osuna, Blanca Garcia-Garcia, Joel Torra, Joao Portugal, Rafael De Prado
Summary: A study in southern Spain found that Sinapis alba populations have developed resistance to the acetolactate synthase-inhibiting herbicide tribenuron-methyl. The resistant populations showed significantly higher resistance to tribenuron-methyl compared to the susceptible population. A mutation in the ALS gene and P450-mediated metabolism were identified as contributing factors to the resistance. Alternative chemical control methods were effective in controlling both the resistant and susceptible populations.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Agronomy
Lei Chen, Gang Gu, Chengxu Wang, Zhufeng Chen, Wei Yan, Man Jin, Gang Xie, Junli Zhou, Xing Wang Deng, Xiaoyan Tang
Summary: Herbicide resistance is valuable for integrated weed management in agriculture, particularly in rice fields where weedy rice poses a challenge. This study obtained three mutant variants of the OsALS gene in rice through ethyl methyl sulfonate mutagenesis, with W-548 M mutant showing high levels of resistance to a broad spectrum of herbicides. The stable inheritance of herbicide resistance in rice lines makes W-548 M mutation a valuable resource for breeding herbicide resistant rice and weed management.
Article
Agronomy
Valeria E. Palmieri, Clarisa E. Alvarez, Hugo R. Permingeat, Valeria E. Perotti
Summary: This study compared various mutations of the Amaranthus palmeri ALS gene in vitro and found that certain mutations led to a decrease in catalytic efficiency and resistance to herbicides. The A122S mutation provided cross-resistance to different families of ALS inhibitors.
PEST MANAGEMENT SCIENCE
(2022)
Article
Agronomy
Barbara Wrzesinska, Tadeusz Praczyk
Summary: Centaurea cyanus, a weed species commonly found in fields with cereals, sugar beet, and maize, shows resistance mainly against acetolactate synthase (ALS) inhibitors. Genetic variability in ALS sequence has been observed among resistant plants, with four amino acid sequence changes identified in some resistant plants compared to susceptible ones. Further research is needed to confirm the significance of these mutations in herbicide resistance development.
Article
Biochemistry & Molecular Biology
Paweena Chuenwarin, Azumi Shimazaki, Masanori Shimizu, Yuko Kobayashi, Masakazu Katsumata, Hirokazu Kobayashi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2016)
Article
Biochemistry & Molecular Biology
Aftab Ahmad, Izumi Kaji, Yukiko Murakami, Nana Funato, Takeshi Ogawa, Masanori Shimizu, Yasuo Niwa, Hirokazu Kobayashi
BIOSCIENCE BIOTECHNOLOGY AND BIOCHEMISTRY
(2009)
Article
Agriculture, Multidisciplinary
Hiroyuki Sakakibara, Takeshi Ogawa, Akiharu Koyanagi, Saori Kobayashi, Toshinao Goda, Shigenori Kumazawa, Hirokazu Kobayashi, Kayoko Shimoi
JOURNAL OF AGRICULTURAL AND FOOD CHEMISTRY
(2009)
Article
Biochemistry & Molecular Biology
Mika Hayashi-Tsugane, Masahiko Maekawa, Qian Qian, Hirokazu Kobayashi, Shigeru Iida, Kazuo Tsugane
JOURNAL OF GENETICS AND GENOMICS
(2011)
Article
Biotechnology & Applied Microbiology
Kiyoshi Kawai, Koichiro Kaku, Norihiko Izawa, Masanori Shimizu, Hirokazu Kobayashi, Tsutomu Shimizu
PLANT BIOTECHNOLOGY
(2010)
Article
Plant Sciences
Masanori Shimizu, Maki Goto, Moeko Hanai, Tsutomu Shimizu, Norihiko Izawa, Hirosuke Kanamoto, Ken-Ichi Tomizawa, Akiho Yokota, Hirokazu Kobayashi
Article
Multidisciplinary Sciences
Katsuhiro Nakanishi, Sanshiro Narimatsu, Shiori Ichikawa, Yuki Tobisawa, Kohta Kurohane, Yasuo Niwa, Hirokazu Kobayashi, Yasuyuki Imai
Article
Multidisciplinary Sciences
Aftab Ahmad, Yasuo Niwa, Shingo Goto, Kyoko Kobayashi, Masanori Shimizu, Sohei Ito, Yumiko Usui, Tsutomu Nakayama, Hirokazu Kobayashi
Article
Multidisciplinary Sciences
Aftab Ahmad, Yasuo Niwa, Shingo Goto, Takeshi Ogawa, Masanori Shimizu, Akane Suzuki, Kyoko Kobayashi, Hirokazu Kobayashi
Article
Multidisciplinary Sciences
Masanori Shimizu, Hideki Kato, Takeshi Ogawa, Aya Kurachi, Yoshiko Nakagawa, Hirokazu Kobayashi
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2010)
Article
Multidisciplinary Sciences
Katsuhiro Nakanishi, Shota Morikane, Shiori Ichikawa, Kohta Kurohane, Yasuo Niwa, Yoshihiro Akimoto, Sachie Matsubara, Hayato Kawakami, Hirokazu Kobayashi, Yasuyuki Imai
SCIENTIFIC REPORTS
(2017)
Article
Biochemistry & Molecular Biology
Katsuhiro Nakanishi, Noriko Mogi, Yuki Kikuchi, Minami Matsuda, Takeshi Matsuoka, Kotome Shiina, Shota Morikane, Kohta Kurohane, Yasuo Niwa, Hirokazu Kobayashi, Yasuyuki Imai
Summary: The study demonstrates that plant-derived secretory component can enhance protease-resistance of SIgA, potentially contributing to its oral application. The reconstituted SIgA with plant-derived SC showed increased resistance to pepsin and trypsin, suggesting a promising alternative for efficient production of orally applicable SIgA.
PLANT MOLECULAR BIOLOGY
(2021)
Article
Chemistry, Medicinal
Shigenori Kumazawa, Satomi Kubota, Haruna Yamamoto, Naoki Okamura, Yasumasa Sugiyama, Hirokazu Kobayashi, Motoyasu Nakanishi, Toshiro Ohta
NATURAL PRODUCT COMMUNICATIONS
(2013)
Article
Biochemistry & Molecular Biology
Mika Hayashi-Tsugane, Masahiko Maekawa, Hirokazu Kobayashi, Shigeru Iida, Kazuo Tsugane
GENES & GENETIC SYSTEMS
(2011)