Review
Polymer Science
Ali M. Alqahtani
Summary: This comprehensive review provides an in-depth analysis of the use of biomaterials in guided tissue and bone regeneration in dental therapeutic interventions. The review explores different types of membranes and biomaterials used in guided bone regeneration procedures, as well as the manufacturing technologies for the scaffold. The significance of guided bone regeneration and the critical role of membranes in successful bone and tissue regeneration are emphasized.
Article
Dentistry, Oral Surgery & Medicine
Mahabba M. Asad, Reem S. Abdelhafez, Raghda Barham, Maram Abdaljaleel, Ban Alkurdi, Sabal Al-Hadidi, Suzan Zalloum, Mohammad M. Ismail, Rula Buqain, Hanan Jafar, Nidaa A. Ababneh
Summary: This study aimed to regenerate the gingival connective tissue in vitro using human gingival fibroblasts cultured in three-dimensional fibrin gel scaffolds. Results showed that culturing the fibroblasts in collagen-stimulating media led to increased cellular viability, proliferation, and production of collagen and other extracellular matrix components. These findings have significant implications for further research on developing compatible scaffolds for gingival soft tissue regeneration and treatment of mucogingival deformities.
ARCHIVES OF ORAL BIOLOGY
(2023)
Article
Biophysics
Victoria L. Abdo, Lina J. Suarez, Lucca Gomes de Paula, Raphael C. Costa, Jamil Shibli, Magda Feres, Valentim A. R. Bara, Martinna Bertolini, Joao Gabriel Silva Souza
Summary: This study investigates the issue of microbial contamination of resorbable membranes, finding that different types of bacteria colonize the exposed membrane surface, thereby affecting the effectiveness of bone regeneration therapy.
COLLOIDS AND SURFACES B-BIOINTERFACES
(2023)
Review
Materials Science, Multidisciplinary
Yuwei Zhu, Lei Zhao, To Ngai
Summary: Periodontal disease can lead to severe degeneration of periodontium tissue, and the prevalence of this disease is increasing worldwide. Guided tissue regeneration (GTR) therapy with advanced multiphasic membranes/scaffolds has been successful in restoring periodontal tissues and functions. This review discusses the recent advancements in the design of multiphasic GTR membranes/scaffolds and their therapeutic efficacy in vitro and in vivo.
MACROMOLECULAR MATERIALS AND ENGINEERING
(2023)
Article
Critical Care Medicine
Alexander P. Larsson, Kristina Briheim, Victor Hanna, Karin Gustafsson, Annika Starkenberg, Hans N. Vintertun, Gunnar Kratz, Johan P. E. Junker
Summary: Transplantation of autologous keratinocytes and fibroblasts seeded on porous gelatin microcarriers stimulates re-epithelialization of wounds, offering a promising candidate for skin transplantation. Future studies will focus on evaluating long-term quality of healing following transplantation.
Article
Cell Biology
Siddharth Shanbhag, Carina Kampleitner, Niyaz Al-Sharabi, Samih Mohamed-Ahmed, Karol Ali Apaza Alccayhuaman, Patrick Heimel, Stefan Tangl, Andreas Beinlich, Neha Rana, Mariano Sanz, Einar K. Kristoffersen, Kamal Mustafa, Reinhard Gruber
Summary: Functionalizing collagen membranes (MEM) with conditioned media (CM) from human bone marrow MSC (MEM-CM) enhanced new bone formation in rat calvarial defects. Lyophilized MEM-CM (CM-LYO) showed superior radiographic and histological outcomes compared to soaking prepared MEM-CM (CM-SOAK), MEM with rat MSC (CEL), and native MEM groups. Proteomic analysis of CM-LYO revealed enriched proteins and biological processes related to bone formation.
Article
Nanoscience & Nanotechnology
Abdel H. Mahmoud, Yuanyuan Han, Renan Dal-Fabbro, Arwa Daghrery, Jinping Xu, Darnell Kaigler, Sarit B. Bhaduri, Jos Malda, Marco C. Bottino
Summary: In this study, highly innovative nanoscale beta-tricalcium phosphate (β-TCP)-laden gelatin methacryloyl/polycaprolactone (GelMA/PCL-TCP) photocrosslinkable composite fibrous membranes were fabricated via electrospinning for guided bone regeneration (GBR). The composite microfibers showed a uniform porous network and successful integration of β-TCP particles. The GelMA/PCL-TCP membranes exhibited enhanced cell attachment, proliferation, mineralization, and osteogenic gene expression in alveolar bone-derived mesenchymal stem cells (aBMSCs) compared to pure PCL and GelMA/PCL. Furthermore, the GelMA/PCL-TCP membrane promoted robust bone regeneration in rat calvarial critical-size defects, demonstrating remarkable osteogenesis compared to PCL and GelMA/PCL groups. Overall, the electrospun GelMA/PCL-TCP composite showed great potential as a promising membrane for guided bone regeneration.
ACS APPLIED MATERIALS & INTERFACES
(2023)
Article
Polymer Science
Yue Gao, Shuai Wang, Biying Shi, Yuxuan Wang, Yimeng Chen, Xuanyi Wang, Eui-Seok Lee, Heng-Bo Jiang
Summary: This review discusses the GTR/GBR materials used in bone/tissue regeneration engineering and the functional properties required for these membranes. While no single modern membrane meets all the necessary characteristics, the development of novel modified materials provides new possibilities in this field.
Article
Chemistry, Physical
Farshid Vahdatinia, Amirarsalan Hooshyarfard, Shokoofeh Jamshidi, Setareh Shojaei, Kishan Patel, Erfan Moeinifard, Rasool Haddadi, Maryam Farhadian, Leila Gholami, Lobat Tayebi
Summary: This study examined the efficacy of a 3D-printed gelatin/elastin/sodium hyaluronate membrane for guided tissue regeneration (GTR) in a critical-size periodontal canine model. The membrane showed the potential to replace collagen membranes for cost-effective GTR therapies.
Article
Nanoscience & Nanotechnology
Davide Porrelli, Mario Mardirossian, Luigi Musciacchio, Micol Pacor, Federico Berton, Matteo Crosera, Gianluca Turco
Summary: The study aimed to enhance the bioactivity of electrospun PCL membranes by coating them with bioactive lactose-modified chitosan (CTL), which can be used for the synthesis and stabilization of silver nanoparticles to provide antibacterial properties. The CTL coating improved MG63 osteoblast adhesion and proliferation, while discouraging biofilm formation by Pseudomonas aeruginosa and Staphylococcus aureus.
ACS APPLIED MATERIALS & INTERFACES
(2021)
Review
Polymer Science
Manuel Toledano-Osorio, Esther Munoz-Soto, Manuel Toledano, Marta Vallecillo-Rivas, Cristina Vallecillo, Pablo Ramos-Garcia, Raquel Osorio
Summary: This study aimed to evaluate the efficacy of palatal mucosa and polymeric soft tissue substitutes in root coverage through a literature review and meta-analysis. The results showed that both coronal advanced flap and tunnel techniques achieved similar levels of root coverage. The only difference was observed in single recessions, where the coronal advanced flap technique provided a higher percentage of root coverage. There was no difference in root coverage when using either an autograft or a polymeric substitute with the tested surgical techniques.
Review
Biochemistry & Molecular Biology
Mathilde Fenelon, Paul Galvez, Daniel Kalbermatten, Paolo Scolozzi, Srinivas Madduri
Summary: The amniotic membrane (AM) is a widely available and cost-effective tissue with unique biological properties. Advanced hybrid materials based on AMs have been developed by combining them with secondary materials. This review focuses on the increasing development of new multilayer composite-based AMs and highlights the benefits of additive manufacturing technologies and electrospinning in expanding their use for tissue engineering and clinical applications. The use of AMs and multilayer composite-based AMs in nerve regeneration is particularly emphasized.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Health Care Sciences & Services
Barbara Sterczala, Kinga Grzech-Lesniak, Olga Michel, Witold Trzeciakowski, Marzena Dominiak, Kamil Jurczyszyn
Summary: The study showed that irradiation with 635 nm and 405 nm lasers can significantly increase the proliferation of gingival fibroblasts by using different wavelengths of lasers for irradiation.
JOURNAL OF PERSONALIZED MEDICINE
(2021)
Article
Engineering, Biomedical
Yingji Mao, Yu Chen, Weifeng Li, Ying Wang, Jingjing Qiu, Yingxiao Fu, Jianzhong Guan, Pinghui Zhou
Summary: This study developed a multilayer nanofibrous membrane that mimics the endogenous cascades of bone regeneration. The membrane promotes migration and osteo-differentiation of bone marrow mesenchymal stem cells and exhibits good biocompatibility and a dual-delivery system, contributing to bone regeneration and vascularization.
ADVANCED HEALTHCARE MATERIALS
(2022)
Review
Materials Science, Multidisciplinary
Ji-Youn Kim, Jun-Beom Park
Summary: Research has shown that coating barrier membranes with different bioactive materials can improve the effectiveness of guided bone/tissue regeneration in cases of periodontal bone defects. Although clinical studies in humans are limited to date, efforts are needed to translate these research findings into clinical practice to confirm the effects of various coating materials.