Article
Immunology
Bo Zhang, Guanyin Zhu, Junqi Liu, Chenghao Zhang, Ke Yao, Xinqi Huang, Xiao Cen, Zhihe Zhao
Summary: The study revealed the cellular heterogeneity of rat gingiva associated with periodontitis and diabetes. It showed that diabetes led to changes in stromal and epithelial cells, promoting immune cell recruitment and pro-inflammatory phenotypes. The study also found enhanced infiltration of neutrophils and M1 macrophages in periodontitis lesions, along with increased cell-cell communications between stromal/epithelial cells and immune cells.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Immunology
Tianle Xu, Hao Zhu, Run Liu, Xinyue Wu, Guangjun Chang, Yi Yang, Zhangping Yang
Summary: The study demonstrates the inhibitory effect of caffeic acid on bacterial growth from clinical mastitis samples, biofilm formation of E. coli, and adherence of E. coli on bMECs. Caffeic acid was also found to attenuate proinflammatory and oxidative responses in E. coli-infected cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Review
Dentistry, Oral Surgery & Medicine
Ana M. Chang, Nutthapong Kantrong, Richard P. Darveau
Summary: The coordination between oral commensal bacteria and the host innate immune response is crucial for maintaining oral homeostasis. Commensal bacteria are speculated to play a host protective role in maintaining health, although little is known about their specific contributions to oral health.
PERIODONTOLOGY 2000
(2021)
Article
Dentistry, Oral Surgery & Medicine
H. Sharawi, O. Heyman, G. Mizraji, Y. Horev, A. Laviv, L. Shapira, S. Yona, A. H. Hovav, A. Wilensky
Summary: Dendritic cells (DCs) play a crucial role in immunity and tolerance induction, including in oral mucosa homeostasis. This study identified four subsets of DCs in healthy gingiva and found dysregulation of gingival DC subsets in periodontal patients, with an impact on cytokine expression. Smoking was also shown to reduce gingival LCs in healthy individuals, potentially contributing to the severity of periodontitis in smokers. These findings lay the groundwork for understanding the role of human DCs in oral immunity and the modulation of immunity in periodontitis.
JOURNAL OF DENTAL RESEARCH
(2021)
Article
Multidisciplinary Sciences
Kumar Sachin Singh, Rishabh Sharma, Poli Adi Narayana Reddy, Prashanthi Vonteddu, Madeline Good, Anjana Sundarrajan, Hyeree Choi, Kar Muthumani, Andrew Kossenkov, Aaron R. Goldman, Hsin-Yao Tang, Maxim Totrov, Joel Cassel, Maureen E. Murphy, Rajasekharan Somasundaram, Meenhard Herlyn, Joseph M. Salvino, Farokh Dotiwala
Summary: Isoprenoids are essential for maintaining cellular functions and IspH is a crucial enzyme in their synthesis pathway. Through structure-guided design, a class of IspH inhibitors with nanomolar potency were identified, showing efficacy against multidrug-resistant bacteria while being relatively non-toxic to mammalian cells. These prodrugs also induce expansion and activation of human V gamma 9V delta 2 T cells, suggesting a potential strategy to limit antibiotic-resistant bacterial populations.
Review
Immunology
Jing Zhang, Ling Chen, Yang Yang, Xiao Liu, Yuan Yuan, Shi-rong Song, Yue Zhao, Jian Mao
Summary: The purpose of this study was to compare the clinical and laboratory characteristics of late-onset sepsis (LOS) caused by Gram-negative and Gram-positive bacteria in preterm neonates and evaluate the diagnostic accuracy of biomarkers. The results showed that LOS caused by Gram-negative bacteria in preterm neonates had higher levels of CRP and lower platelet counts. Therefore, platelet count and CRP values may be useful biomarkers to differentiate sepsis caused by Gram-negative and Gram-positive bacteria in preterm neonates.
INTERNATIONAL IMMUNOPHARMACOLOGY
(2023)
Article
Dentistry, Oral Surgery & Medicine
M. Arce, M. Rodriguez-Pena, J. Espinoza-Arrue, R. A. Godoy, M. Reyes, T. Kajikawa, T. Greenwell-Wild, G. Hajishengallis, L. Abusleme, N. Moutsopoulos, N. Dutzan
Summary: Periodontitis is a common inflammatory disease characterized by tissue destruction. This study found that cytokines such as IL-6 and IL-23, which are associated with tissue destruction, use a signaling pathway mediated by STAT3. STAT3 activation was found to be increased in epithelial cells and T cells during periodontitis.
JOURNAL OF DENTAL RESEARCH
(2023)
Review
Immunology
Mi Zhou, Dana T. Graves
Summary: Periodontitis is caused by an immune response to bacterial dysbiosis, leading to bone resorption and limited bone repair processes.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Food Science & Technology
Zonghong Luo, Guanhong Chang, Yang Liu, Ke Ni, Ting Zhou, Xin Lv, Jiangtao Yu, Junqing Bai, Xin Wang
Summary: This study evaluated the effect of electron beam irradiation on the inactivation of Gram-negative bacteria and the destruction of extracellular polymers (EPS) in biofilms. The results showed that electron beam irradiation significantly reduced the number of viable bacteria both in vitro and on the surface of seafood samples, and it damaged the plasma membrane of bacterial cells by increasing reactive oxygen species (ROS) levels. Furthermore, the nucleic acids of the bacteria were also significantly damaged, while the primary protein structure remained unchanged.
LWT-FOOD SCIENCE AND TECHNOLOGY
(2022)
Article
Infectious Diseases
Antonella Migliaccio, Maria Stabile, Maria Bagattini, Maria Triassi, Rita Berisio, Eliana De Gregorio, Raffaele Zarrilli
Summary: Resveratrol can reverse tolerance and restore susceptibility to CHX and BZK in the majority of microorganisms causing HAI. The efflux pump inhibitor reduced the MIC and MBC of CHX and BZK in most strains, while resveratrol dose-dependently decreased the MIC and MBC of the biocides.
Review
Gastroenterology & Hepatology
Yingping Xiao, Huicong Zou, Jingjing Li, Tongxing Song, Wentao Lv, Wen Wang, Zhenyu Wang, Shiyu Tao
Summary: Quorum sensing is a vital molecular signaling mechanism in bacteria that regulates microbial community behavior and host cell function. Blocking quorum sensing signaling molecules may present opportunities for combating diseases.
Article
Microbiology
M. Znaidia, Y. de Souza-Angelo, S. Letoffe, I. Staropoli, L. Grzelak, J. M. Ghigo, O. Schwartz, N. Casartelli
Summary: Microbial translocation is associated with systemic immune activation in HIV-1 disease. Some bacteria can activate T cells and enhance HIV-1 replication, suggesting a role of bacterial factors in promoting T cell activation and HIV-1 replication.
MICROBIOLOGY SPECTRUM
(2023)
Article
Dentistry, Oral Surgery & Medicine
Fabio R. M. Leite, Rodrigo Lopez, Holger J. Moller, Gustavo G. Nascimento
Summary: This study aimed to describe cytokine profiles in periodontal healing after periodontal therapy in smokers. The results suggest that different smoking trajectories have distinct effects on cytokine expression, which in turn influence periodontal repair.
JOURNAL OF PERIODONTOLOGY
(2023)
Review
Cell Biology
He Liu, Zhen Hu, Mengyang Li, Yi Yang, Shuguang Lu, Xiancai Rao
Summary: G(+) bacterial infections pose a significant burden and require urgent development of antimicrobial agents due to the prevalence of multidrug-resistant G(+) bacteria. Endolysins, phage-encoded enzymes, are promising alternatives for combating resistance due to their low bacterial resistance. This review classifies and summarizes the mechanisms, efficacy, and advantages of endolysins as antibacterial drug candidates targeting G(+) bacteria. Safety, challenges, and potential solutions are also addressed, with indications that endolysin-based drugs will be approved in the near future.
JOURNAL OF BIOMEDICAL SCIENCE
(2023)
Article
Chemistry, Multidisciplinary
Guocheng Xie, Sijia Gao, Jinzhao Ou, Meng Zhu, Man Wu, Xiaoyan Ju, Zhuang Li, Ye Tian, Zhongwei Niu
Summary: By conjugating peptides onto tobacco mosaic virus, the resulting nanoparticles show significantly enhanced antibacterial effects against Gram-negative bacteria. The high osmotic pressure and reactive oxygen species generated by the peptide-TMV cause the death of Escherichia coli, while also downregulating biofilm-related genes to inhibit biofilm formation. This study opens up new possibilities for combating Gram-negative bacteria-related infections.
Article
Microbiology
Akira Horiuchi, Eitoyo Kokubu, Takenobu Warita, Kazuyuki Ishihara
Article
Biochemistry & Molecular Biology
Kazuki Izawa, Kazuko Okamoto-Shibayama, Daichi Kita, Sachiyo Tomita, Atsushi Saito, Takashi Ishida, Masahito Ohue, Yutaka Akiyama, Kazuyuki Ishihara
Summary: The study conducted a metagenomic analysis of subgingival plaque samples from Japanese individuals with and without periodontitis, revealing significant differences in the composition of Bacteroides and Mycobacterium genera between healthy and diseased sites, as well as in the relative abundance of functional gene categories such as carbohydrate metabolism and amino acid metabolism. These results provide important insights into the dysbiosis-induced changes in taxonomic and functional gene category abundance during periodontal disease progression.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Dentistry, Oral Surgery & Medicine
Saki Nakane, Kentaro Imamura, Rio Hisanaga, Kazuyuki Ishihara, Atsushi Saito
Summary: The study demonstrated that CTLA-4-Ig can effectively inhibit bone resorption in periodontitis by affecting osteoclast differentiation and activity, potentially through the regulation of the NF-kappa B pathway and PP2A expression.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Dentistry, Oral Surgery & Medicine
Masahiro Shimogishi, Takayasu Watanabe, Masaki Shibasaki, Takahiko Shiba, Keiji Komatsu, Takashi Nemoto, Kazuyuki Ishihara, Yoshio Nakano, Takanori Iwata, Shohei Kasugai, Ichiro Nakagawa
Summary: The study found that the bacterial composition in the peri-implant sulcus was similar among patients at 1 and 2 weeks post-uncovering, with predominance of Firmicutes and Proteobacteria. However, the composition became more diverse and significantly different at 4 and 6 weeks. This suggests that the peri-implant microbiota is established early after implant uncovering, but changes over time and varies between patients.
JOURNAL OF PERIODONTAL RESEARCH
(2021)
Article
Immunology
Eitoyo Kokubu, Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Shuichi Nakamura, Kazuyuki Ishihara
Summary: The study demonstrates that dentilisin plays a role in the crawling-dependent surface spreading of T. denticola by affecting its motility speed and efficiency, as evidenced by the smaller colony area, lower crawling velocity, and reduced crawling efficiency observed in dentilisin-deficient strains compared to wild-type and Msp mutant strains.
MICROBIOLOGY AND IMMUNOLOGY
(2021)
Article
Microbiology
Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Eitoyo Kokubu, Kazuyuki Ishihara
Summary: Among the bacteria inhabiting the human oral cavity, Capnocytophaga ochracea plays a significant role in the early phase of dental plaque formation on tooth surfaces. Its response to oxidative stress may be linked to the hydrogen peroxide-sensing transcriptional regulator OxyR.
Article
Dermatology
Yurie Akiyama, Masaki Minabe, Junichiro Inada, Takeshi Nomura, Shinichi Takahashi, Kazuyuki Ishihara, Michiyoshi Kouno
Summary: The study found that the oral microbiome may influence the clinical course of patients with palmoplantar pustulosis (PPP) after treatment of dental focal infections, with improved patients showing higher oral microbial diversity. These findings could serve as a predictive factor for clinical responsiveness to dental focal infection treatment in PPP patients.
JOURNAL OF DERMATOLOGICAL SCIENCE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Eitoyo Kokubu, Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Kazuyuki Ishihara
Summary: This study investigated the effects of T. denticola virulence factors dentilisin and Msp on epithelial cells. The results showed that T. denticola disrupts the function of epithelial cells by hydrolyzing intercellular junction proteins and focal adhesion proteins, inhibiting cell migration and healing.
BULLETIN OF TOKYO DENTAL COLLEGE
(2022)
Article
Dentistry, Oral Surgery & Medicine
Yuri Miyai-Murai, Kazuko Okamoto-Shibayama, Toru Sato, Yuichiro Kikuchi, Eitoyo Kokubu, Jan Potempa, Kazuyuki Ishihara
Summary: The Msp protein complex and the serine protease dentilisin are the best-characterized virulence factors in Treponema denticola, the major etiological agent of chronic periodontitis. In addition, the cysteine protease dentipain also contributes to pathogenicity, but its secretion, processing, cellular localization, and role in T. denticola virulence are not fully understood. This study found that dentipain is located in the outer sheath derived from T. denticola dentilisin- and Msp-deficient mutants, suggesting that dentilisin and Msp are involved in the secretion and maturation of dentipain. Inactivation of the dentipain gene slowed the growth of T. denticola, and the mutant strain showed altered gene expression, increased hydrophobicity, reduced autoaggregation activity, and decreased adhesion to IgG.
MOLECULAR ORAL MICROBIOLOGY
(2023)
Article
Microbiology
Yukiko Yamaguchi-Kuroda, Yuichiro Kikuchi, Eitoyo Kokubu, Kazuyuki Ishihara
Summary: This study investigated the effect of diffusible signaling molecules from P. gingivalis on F. nucleatum and revealed that these molecules induce metabolic changes in F. nucleatum, including an increase in polysaccharide synthesis and a reduction in de novo synthesis of purine and pyrimidine. These changes may accelerate biofilm formation by F. nucleatum with P. gingivalis and could serve as potential therapeutic targets for preventing dysbiosis.
JOURNAL OF ORAL MICROBIOLOGY
(2023)
Article
Medicine, General & Internal
Takahiko Shiba, Takayasu Watanabe, Keiji Komatsu, Tatsuro Koyanagi, Takashi Nemoto, Yujin Ohsugi, Yasuyuki Michi, Sayaka Katagiri, Yasuo Takeuchi, Kazuyuki Ishihara, Takanori Iwata
Summary: A successful case of non-surgical treatment for peri-implantitis with horizontal bone resorption in a 68-year-old male patient was reported, showing decreased probing depth and recovery of bacterial composition at both sites. The clinical condition remained stable for 7 years after treatment.
SAGE OPEN MEDICAL CASE REPORTS
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kazuko Okamoto-Shibayama, Takenobu Warita, Eitoyo Kokubu, Daichi Kita, Yuichiro Kikuchi, Kazuyuki Ishihara
Summary: The study found that the hyalin-like protein in Capnocytophaga ochracea is involved in gliding activity, but its relationship with biofilm formation is not significant.
BULLETIN OF TOKYO DENTAL COLLEGE
(2021)
Article
Dentistry, Oral Surgery & Medicine
Kazuko Okamoto-Shibayama, Ayumi Yoshida, Kazuyuki Ishihara
Summary: This study demonstrated that resveratrol has inhibitory effects on the formation of Candida albicans biofilms at developmental, sustained, and mature stages. The antifungal activity of resveratrol shows potential as a treatment and prevention tool against Candida albicans by inhibiting the growth of existing or forming biofilms.
BULLETIN OF TOKYO DENTAL COLLEGE
(2021)
Article
Microbiology
Kazuyuki Ishihara, Yuki Arai, Yuichiro Kikuchi, Kazuko Okamoto-Shibayama, Eitoyo Kokubu, Seikou Shintani
JOURNAL OF ORAL MICROBIOLOGY
(2020)
Article
Dentistry, Oral Surgery & Medicine
Yutaroh Satoh, Kazuyuki Ishihara
JOURNAL OF ORAL BIOSCIENCES
(2020)