Review
Cell Biology
Yuka Nagata, Ryo Suzuki
Summary: Mast cells (MCs) have different roles in allergic responses, and the interactions between antigens and immunoglobulin E (IgE) can trigger diverse responses in MCs. The clustering of high-affinity IgE receptor Fc epsilon RI is crucial for signal transduction and determines the quality of MC responses. Antigen properties precisely regulate Fc epsilon RI dynamics, which can result in different outcomes by switching the intracellular signaling pathway, suggesting that antigen properties can control MC responses both qualitatively and quantitatively.
Article
Immunology
Mohammed Zghaebi, Maria Byazrova, Sabine Flicker, Sergio Villazala-Merino, Nicholas J. Campion, Victoria Stanek, Aldine Tu, Heimo Breiteneder, Alexander Filatov, Musa Khaitov, Verena Niederberger-Leppin, Julia Eckl-Dorna, Rudolf Valenta
Summary: A new sequential gating strategy was developed to detect cells bearing the IgE BCR on their surface, using a monoclonal anti-IgE antibody. This strategy allowed for the clear identification of allergen-specific IgE(+) memory B cells and plasma cells in human PBMCs, offering new insights into allergic responses.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Immunology
Yuping Wang, Tengkai Wang, Meijuan Cai, Shuzhen Zhu, Lijun Song, Qian Wang
Summary: The study found that in patients with SLE, the expression of Fc epsilon RI alpha increased while the level of anti-IgE decreased. Additionally, in the CHO3D10 cell line, Fc epsilon RI alpha was found to exist in both monomer and various polymer forms. Mast cell-associated Fc epsilon RI alpha plays a role in the inflammatory lesions of SLE.
IMMUNITY INFLAMMATION AND DISEASE
(2022)
Article
Cell Biology
Ashley Wagner, Syed Benazir Alam, Marianna Kulka
Summary: This study compared the response of different mast cell models to common stimuli and found that factors such as source, age, and biological sex play an important role in phenotypic outcomes and may explain the variability in their function.
CELLULAR IMMUNOLOGY
(2023)
Article
Multidisciplinary Sciences
Donald MacGlashan
Summary: This study found that released FceRI-alpha accounted for 7% of total surface FceRI before the reaction, with a molecular weight of approximately 54 kD. The release of FceRI-alpha was found to be independent of internalized processing and starting density of FceRI, and was more effective with non-IgE-dependent stimuli.
Article
Biochemistry & Molecular Biology
Tsubasa Ashikari, Masakazu Hachisu, Kazuki Nagata, Daisuke Ando, Yuki Iizuka, Naoto Ito, Kandai Ito, Yuki Ikeda, Hiroki Matsubara, Takuya Yashiro, Kazumi Kasakura, Chiharu Nishiyama
Summary: Mast cells play a significant role in IgE-mediated immunoresponses, and salicylaldehyde, identified as an inhibitor of mast cell activation, shows potential as an antiallergic compound.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Allergy
Kirti J. Johal, Kristin L. Chichester, Eric T. Oliver, Kelly C. Devine, Anja P. Bieneman, John T. Schroeder, Donald W. MacGlashan, Sarbjit S. Saini
Summary: The study suggests that the baseline basophil count and functional phenotype may predict responsiveness to omalizumab in treating chronic spontaneous urticaria. However, changes in basophil IgE-based histamine release, surface IgE, or Fc epsilon RI do not correlate with the kinetics of improvement in clinical symptoms during therapy.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Sathisha Upparahalli Venkateshaiah, Madhavi Rayapudi, Hemanth Kumar Kandikattu, Chandra Sekhar Yadavalli, Anil Mishra
Summary: This study demonstrates that IL-15 responsive T cells and IgE receptors can serve as novel non-invasive biomarkers for distinguishing between EoE and GERD. Additionally, analysis of blood mRNA levels reveals certain receptors significantly reduced in EoE patients, which can be used as predictors for EoE.
CLINICAL IMMUNOLOGY
(2021)
Article
Immunology
Robert W. E. Crozier, Val A. Fajardo, Adam J. MacNeil
Summary: This study aimed to evaluate the potential therapeutic target of GSK3 in allergic inflammation. The results revealed that GSK3 inhibition reduced ROS production and degranulation, and decreased the production/release of pro-inflammatory mediators. Additionally, CHIR99021-mediated GSK3 inhibition altered the pro-inflammatory phenotype of mast cells.
EUROPEAN JOURNAL OF IMMUNOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Greer K. Arthur, Glenn Cruse
Summary: Mast cells are immune cells involved in allergic inflammatory response. This review focuses on IgE-mediated activation and discusses the structure and function of Fc epsilon RI, as well as the role of Fc epsilon RI beta in regulating mast cell function and signaling. Current and emerging approaches to target IgE and Fc epsilon RI signaling are discussed, along with the potential therapeutic use of alternative splicing of Fc epsilon RI beta.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Review
Immunology
Kang Chen, Yujing Hao, Mauricio Guzman, Genxia Li, Andrea Cerutti
Summary: An increasing number of human diseases, including allergies, infections, inflammation, and cancer, involve basophils. Basophils, traditionally viewed as rare leukocytes present only in the circulation, have recently emerged as important players in immune responses. Their functions are regulated by immunoglobulins, allowing them to integrate diverse adaptive and innate immunity signals. This article discusses recent advances in understanding antibody-mediated basophil responses and proposes strategies for treating basophil-associated disorders.
TRENDS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Martin Metz, Gordon Sussman, Remi Gagnon, Petra Staubach, Tonny Tanus, William H. Yang, Jeremy J. Lim, Holly J. Clarke, Joshua Galanter, Leslie W. Chinn, Tom Chu, Anastasia Teterina, Tracy Burgess, D. James Haddon, Timothy T. Lu, Marcus Maurer
Summary: The study investigated the efficacy of Fenebrutinib in treatment of chronic spontaneous urticaria patients, showing dose-dependent improvements in disease activity, especially in patients with refractory type IIb autoimmunity. This supports the potential use of BTK inhibition in antihistamine-refractory CSU.
Article
Allergy
Cynthia Kanagaratham, Tahereh Derakhshan, Yasmeen S. El Ansari, Kameryn N. Furiness, Eleanor Hollers, Mats Keldsen, Hans C. Oettgen, Daniel F. Dwyer
Summary: In this study, the inhibitory effects of IgG:Fc?RIIb signaling on MC responses to IgE were assessed using complementary transcriptomic and functional approaches. The results showed that IgG signaling via Fc?RIIb has a suppressive effect on IgE-induced MC transcription, especially genes related to bone marrow-derived MCs activation. These findings are important for understanding the regulatory mechanisms of IgG on IgE-induced MC responses.
JOURNAL OF ALLERGY AND CLINICAL IMMUNOLOGY
(2023)
Article
Immunology
Satomi Mishima, Marin Sakamoto, Hikaru Kioka, Yuka Nagata, Ryo Suzuki
Summary: This study comprehensively examined the role of VAMP3 in mast cell function and the molecular mechanisms underlying it. The results suggest that VAMP3 mediates granule fusion and promotes granule exocytosis in mast cells. The loss of VAMP3 disrupts plasma membrane homeostasis and enhances Fc epsilon RI-mediated signal transduction. VAMP3 plays a multifunctional role in complex secretory responses in mast cells.
FRONTIERS IN IMMUNOLOGY
(2022)
Article
Critical Care Medicine
Angelica Tiotiu, Yusef Badi, Nazanin Zounemat Kermani, Marek Sanak, Johan Kolmert, Craig E. Wheelock, Philip M. Hansbro, Sven-Erik Dahlen, Peter J. Sterk, Ratko Djukanovic, Yike Guo, Sharon Mumby, Ian M. Adcock, Kian Fan Chung
Summary: By analyzing gene signatures of MC activation, we can identify phenotypes of severe asthma and indicate that MCs can have distinct transcriptional phenotypes associated with specific clinical phenotypes.
AMERICAN JOURNAL OF RESPIRATORY AND CRITICAL CARE MEDICINE
(2022)
Review
Biochemistry & Molecular Biology
Jwala Sivaccumar, Annamaria Sandomenico, Luigi Vitagliano, Menotti Ruvo
Summary: This study traces the most significant findings that have been awarded over the years, reports on important clinical applications, and highlights the latest emerging therapeutic trends in monoclonal antibodies.
CURRENT MEDICINAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Mariarosaria Conte, Rosanna Palumbo, Alessandra Monti, Elisabetta Fontana, Angela Nebbioso, Menotti Ruvo, Lucia Altucci, Nunzianna Doti
Summary: The AIF/CypA complex has lethal activity in rodent models of acute brain injury, but an AIF peptide has been found to restore cell survival and prevent brain injury. This study shows that the peptide can also protect against apoptosis in PD cells, suggesting the AIF/CypA complex as a potential target for developing new PD therapies.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Andrea Arena, Eugenia Belcastro, Antonella Accardo, Annamaria Sandomenico, Olivia Pagliarosi, Elisabetta Rosa, Stefania Petrini, Libenzio Adrian Conti, Ezio Giorda, Tiziana Corsetti, Riccardo Schiaffini, Giancarlo Morelli, Alessandra Fierabracci
Summary: Autoimmune endocrine disorders such as type 1 diabetes and thyroiditis currently rely on hormone replacement therapy, highlighting the need for personalized immunotherapeutic strategies targeting T and B lymphocytes. This study improved upon a personalized immunotherapeutic approach by functionalizing liposomes with Rituximab antibody, specifically targeting B lymphocytes in conditions like type 1 diabetes. The results showed that these functionalized liposomes had a significant inhibitory effect on the function of B lymphocytes, suggesting potential applicability for personalized immunotherapy in other autoimmune disorders where B lymphocytes are the primary pathogenic immune cells.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Marta Gogliettino, Emanuela Iaccarino, Carmela Fusco, Andrea Caporale, Menotti Ruvo, Gianna Palmieri, Ennio Cocca
Summary: APEH is a crucial protease involved in protein degradation, and the presence of sulphoxide-modified methionines plays an important role in its endopeptidase activity.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Biochemistry & Molecular Biology
Pasqualina Liana Scognamiglio, Caterina Vicidomini, Francesco Fontanella, Claudio De Stefano, Rosanna Palumbo, Giovanni N. Roviello
Summary: Benzofuran derivatives have potential as drugs due to their ability to interact with nucleic acids, interfere with amyloid peptide aggregation, and affect cancer cell cycles. This study investigated the binding ability of two benzofuran compounds (BF1 and BDF1) with bovine serum albumin as a model protein. The results showed that BF1 had a higher affinity for protein binding, leading to changes in protein structure and thermal stability compared to BDF1.
Article
Biology
Donata Molisso, Mariangela Coppola, Martina Buonanno, Ilaria Di Lelio, Simona Maria Monti, Chiara Melchiorre, Angela Amoresano, Giandomenico Corrado, John Paul Delano-Frier, Andrea Becchimanzi, Francesco Pennacchio, Rosa Rao
Summary: Prosystemin is not only a precursor of Systemin peptide, but also contains other biologically active regions. Experimental results show that truncated Prosystemin can promote defense barriers in tomato plants through a hormone-independent defense pathway, and provide protection against pests and pathogens.
Article
Biochemistry & Molecular Biology
Marian Vincenzi, Flavia Anna Mercurio, Concetta Di Natale, Rosanna Palumbo, Luciano Pirone, Sara La Manna, Daniela Marasco, Emilia Maria Pedone, Marilisa Leone
Summary: This study describes the design and functional evaluation of KRI3 analogues that bind to Ship2-Sam. The importance of aromatic interactions for the association with Ship2-Sam is highlighted. Additionally, preliminary cell-based assays demonstrate the cellular uptake capability of KRI3.
BIOORGANIC CHEMISTRY
(2022)
Editorial Material
Biochemistry & Molecular Biology
Simona Maria Monti, Giuseppina De Simone, Emma Langella
Review
Chemistry, Medicinal
Rosanna Palumbo, Daniela Omodei, Caterina Vicidomini, Giovanni N. Roviello
Summary: Willardiine is a nonprotein amino acid that acts as a partial agonist of ionotropic glutamate receptors, specifically the non-NMDA receptors of L-glutamate. Its analogues and derivatives have been extensively studied for their binding affinities and effects on brain regions. Ligands for AMPA and kainate receptors are of particular interest as potential neurodrugs due to their role in neurological diseases. Willardiine and its analogues also hold significance in the field of peptide chemistry, specifically for nucleopeptide development and various biotechnological applications.
Article
Chemistry, Analytical
Sarassunta Ucci, Sara Spaziani, Giuseppe Quero, Patrizio Vaiano, Maria Principe, Alberto Micco, Annamaria Sandomenico, Menotti Ruvo, Marco Consales, Andrea Cusano
Summary: Lab-on-fiber (LoF) optrodes offer advantages for real-time and label-free detection of biomarkers. Improving immobilization strategies using hinge carbohydrates significantly enhances the detection capability. By optimizing and validating the LoF platform, detection of the cancer-related biomarker Cripto-1 has been greatly improved.
Article
Biochemistry & Molecular Biology
Annamaria Sandomenico, Alessia Ruggiero, Emanuela Iaccarino, Angela Oliver, Flavia Squeglia, Miguel Moreira, Luciana Esposito, Menotti Ruvo, Rita Berisio
Summary: CD59 is an abundant protein in humans that protects cells from damage by inhibiting the complement system. It inhibits the assembly of the Membrane Attack Complex (MAC) and is incorporated by pathogenic viruses like HIV-1 to escape complement-mediated virolysis. CD59 is also overexpressed in cancer cells to resist complement attack. In this study, bioinformatics and computational tools were used to identify CD59 interactions and design paratope-mimicking peptides that target CD59.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Review
Biochemistry & Molecular Biology
Monica-Cornelia Sardaru, Narcisa-Laura Marangoci, Rosanna Palumbo, Giovanni N. Roviello, Alexandru Rotaru
Summary: In this article, recent advancements in fluorescent and Raman imaging using oligodeoxyribonucleotide (ODN)-based nanoparticles and nanostructures are reviewed, which show promise as effective tools for live-cell analysis. These nanodevices can be used to investigate a vast number of biological processes occurring at various levels, and have contributed to significant advancements in understanding the role of specific analytes in pathological processes and diagnosing health conditions. The literature reports predict that the development of ODN-based fluorescent and Raman probes will continue in the near future, leading to novel applications in therapeutic and diagnostic strategies.
Article
Biology
Emma Langella, Davide Esposito, Simona Maria Monti, Claudiu T. Supuran, Giuseppina De Simone, Vincenzo Alterio
Summary: Carbonic anhydrases are enzymes involved in an important physiological reaction of converting CO2 to bicarbonate ion. They play crucial roles in various physiological and pathological processes in humans, making them potential targets for therapeutic interventions. This study investigates a class of carbonic anhydrase inhibitors, providing insights into their mechanism of action and potential for drug design.
Article
Chemistry, Medicinal
Jwala P. Sivaccumar, Emanuela Iaccarino, Angela Oliver, Maria Cantile, Pierpaolo Olimpieri, Antonio Leonardi, Menotti Ruvo, Annamaria Sandomenico
Summary: Drug development has shifted toward personalized treatments targeting specific therapeutic targets in individual patients. Monoclonal antibodies (mAbs) and their surrogates, such as Fabs, are increasingly used for their ability to bind and inhibit therapeutic targets. In this study, a recombinant humanized Fab of a murine mAb (3D1) was prepared and characterized, showing potential for targeting Nodal-positive cancer cells.
Article
Chemistry, Multidisciplinary
Pasqualina Liana Scognamiglio, Claudia Riccardi, Rosanna Palumbo, Thomas F. Gale, Domenica Musumeci, Giovanni N. Roviello
Summary: The design, synthesis and characterization of a novel nucleoamino acid derivative named TrpT were described. The self-assembly of TrpT into supramolecular networks in aqueous solution was demonstrated. TrpT nanoaggregates showed good stability, spherical-shaped assemblies, and the ability to bind curcumin. TrpT also interacted with the serum protein BSA and showed high stability in human serum.
JOURNAL OF NANOSTRUCTURE IN CHEMISTRY
(2023)