Article
Environmental Sciences
Kurt A. Gust, Karl J. Indest, Guilherme Lotufo, Steven J. Everman, Carina M. Jung, Mark L. Ballentine, Allison V. Hoke, Bintu Sowe, Aarti Gautam, Rasha Hammamieh, Qing Ji, Natalie D. Barker
Summary: The study suggests that munition exposure can alter tadpole skin microbiome composition and affect the transcriptional profiles in the amphibian host, potentially impacting the host's health and immune status. The findings indicate direct effects of munitions exposure on the skin microbiome, as well as possible indirect effects on microbial flora. The research highlights the importance of understanding the potential impacts of munition exposure on amphibian health and disease resistance.
ENVIRONMENTAL RESEARCH
(2021)
Article
Ecology
Alexander J. Douglas, Laura A. Hug, Barbara A. Katzenback
Summary: Different bacterial skin microbiome compositions were found in North American wood frogs based on seasons and locations, with no significant effect of sex on microbiota diversity observed. The study indicates significant seasonal variation in wood frog skin microbiome structure, while the average diversity per frog remained consistent.
Article
Biochemistry & Molecular Biology
Wenjie Wang, Wanqing Yang, Shouying Du, Xinping Xi, Chengbang Ma, Lei Wang, Mei Zhou, Tianbao Chen
Summary: Bioactive proteins secreted by amphibian skin glands exhibit antimicrobial and antibiofilm activities, offering potential for treating antibiotic-resistant infections. The antimicrobial peptide temporin-FL and its analogues show potent activity against MRSA, with enhanced efficiency and lower cytotoxicity on mammalian cells. These peptides disrupt bacterial membranes, block biofilm formation, and provide insights for developing new drugs for MRSA infections.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2021)
Article
Immunology
Han Liu, Xiaopu Guo, Tangwei Yi, Yihan Zhu, Xinyi Ren, Renxian Guo, Yi Dai, Shaohui Liang
Summary: The tree frog Hyla annectans in the southwestern plateau area of China may possess novel peptides with anti-inflammatory and antioxidant properties, which can protect the skin from UV-induced damage by modulating stress signaling pathways.
FRONTIERS IN IMMUNOLOGY
(2021)
Article
Biochemistry & Molecular Biology
Chun Xie, Yan Fan, Saige Yin, Yilin Li, Naixin Liu, Yixiang Liu, Longjun Shu, Zhe Fu, Yinglei Wang, Yue Zhang, Xiaojie Li, Ying Wang, Jun Sun, Xinwang Yang
Summary: A novel amphibian-derived peptide OS-LL11 has been found to exhibit anti-photodamage activity by maintaining skin health and protecting against UV radiation damage through various mechanisms.
JOURNAL OF PHOTOCHEMISTRY AND PHOTOBIOLOGY B-BIOLOGY
(2021)
Article
Agriculture, Dairy & Animal Science
Haijun Wei, Longwu Geng, Xinchi Shang, Guo Hu, Peixian Luan, Wei Xu
Summary: The background color has an impact on the welfare of captive brown frogs. The study found that brown frogs prefer black backgrounds, regardless of feeding and time of day. Therefore, it is recommended to use more black backgrounds for captive brown frogs to provide a suitable living environment.
APPLIED ANIMAL BEHAVIOUR SCIENCE
(2023)
Article
Environmental Sciences
C. E. Robinson, C. K. Elvidge, R. A. Frank, J. V. Headley, L. M. Hewitt, A. G. Little, S. A. Robinson, V. L. Trudeau, I. J. Vander Meulen, D. M. Orihel
Summary: This study investigates the effects of organic compounds in oil sands process-affected water on wood frogs. The results show that these compounds have no significant effect on mating behavior and fertility, but they decrease offspring viability and development, which may impact the recruitment of native amphibian populations.
ENVIRONMENTAL POLLUTION
(2023)
Article
Developmental Biology
Svetlana A. Kaurova, Victor K. Uteshev, Andrew B. Gapeyev, Natalia V. Shishova, Edith N. Gakhova, Robert K. Browne, Ludmila I. Kramarova
Summary: The successful cryopreservation of testicular spermatozoa of the common frog Rana temporaria was described, with results showing a significant decrease in quality after 4 days of storage when treated with DMF. DMSO had a toxic effect on the spermatozoa. Low levels of DNA fragmentation were observed.
REPRODUCTION FERTILITY AND DEVELOPMENT
(2021)
Review
Cell Biology
Igor M. Souza-Silva, U. Muscha Steckelings, Kasper Bostlund Assersen
Summary: This article reviews the role of two vasoactive peptides, adiponectin and bradykinin, in skin homeostasis. Adiponectin, mainly found in fat depots, can exert paracrine effects in various skin cells. The potential involvement of adiponectin in skin disorders such as psoriasis, atopic dermatitis, and melanogenesis is discussed. The kallikrein-kinin system, including bradykinin, is also found expressed in the skin and may contribute to wound healing, psoriasis, and melanoma.
AMERICAN JOURNAL OF PHYSIOLOGY-CELL PHYSIOLOGY
(2023)
Article
Biology
Myra C. Hughey, Robin Warne, Alexa Dulmage, Robyn E. Reeve, Grace H. Curtis, Kourtnie Whitfield, Danna M. Schock, Erica Crespi
Summary: This study investigated how increasing salinization and nutritional algae influenced gut bacterial assembly, host physiology, and responses to ranavirus exposure in larval wood frogs. The results showed that elevating salinity and supplementing with algae increased larval growth and ranavirus loads. However, larvae given algae did not exhibit stress responses observed in larvae fed a basic diet, indicating a potentially adaptive stress response reversal. Algal supplementation also reduced gut bacterial diversity, with higher relative abundances of Firmicutes associated with host metabolism and endocrine function regulation.
PHILOSOPHICAL TRANSACTIONS OF THE ROYAL SOCIETY B-BIOLOGICAL SCIENCES
(2023)
Article
Biochemical Research Methods
Tatiana Yu. Samgina, Irina D. Vasileva, Polonca Trebse, Gregor Torkar, Alexey K. Surin, Zhaowei Meng, Roman A. Zubarev, Albert T. Lebedev
Summary: Skin secretion of amphibians contains peptides that play a crucial role in defending against pathogens and predators. In this study, mass spectrometry was used to analyze the skin peptidome of Rana arvalis species collected in Central Slovenia and compare it with the peptidome of the Moscow population. Several novel peptides were identified, and their potential as biomarkers was suggested. The study highlights the effectiveness of using MS3 tools in proteomics/peptidomics.
JOURNAL OF THE AMERICAN SOCIETY FOR MASS SPECTROMETRY
(2022)
Article
Environmental Sciences
Elena Adams, Verena Gerstle, Tobias Schmitt, Carsten A. Bruhl
Summary: The study found that co-formulants and adjuvants in pesticide formulations can affect aquatic and terrestrial toxicity for amphibians, with different substances having varying effects on larvae and juveniles. It is important to consider the presence and amount of co-formulants and adjuvants in environmental risk assessments for amphibians.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Review
Cell Biology
Xiakun Wang, Hongcheng Duan, Min Li, Wei Xu, Lin Wei
Summary: Wound-healing-promoting peptides derived from amphibians, particularly salamanders and frogs, have been characterized and shown to effectively accelerate skin wound healing and photodamage repair. They promote the proliferation and migration of keratinocytes and fibroblasts, recruit immune cells to the wound site, and regulate immune responses, thereby playing a crucial role in wound healing.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Physics, Atomic, Molecular & Chemical
T. Y. Samgina, I. D. Vasilieva, A. Yu Kozhevnikov, Zhaowei Meng, R. A. Zubarev, A. T. Lebedev
Summary: Amphibians, the oldest class of vertebrates, possess a unique weapon in stress conditions - a peptide cocktail secreted by their skin glands. These peptides have a potential as future pharmaceuticals due to their different mechanism of interaction with pathogenic microbiota compared to modern antibiotics. High resolution tandem mass spectrometry was used to establish the skin peptidome of Rana temporaria frog population from Arkhangelsk, Russia. The analysis revealed 34 sequenced peptides, including five novel ones. Temporins were identified as the most variable peptide family responsible for the adaptation of Rana temporaria to their habitat conditions. The EThcD MS3 fragmentation method demonstrated its excellent ability to analyze intact disulfide-containing peptides, cyclizing peptides, and isomeric residues.
INTERNATIONAL JOURNAL OF MASS SPECTROMETRY
(2023)
Article
Biochemistry & Molecular Biology
Lei Zhang, Guangshuai Liu, Tian Xia, Xiufeng Yang, Guolei Sun, Chao Zhao, Chunzhu Xu, Honghai Zhang
Summary: The study analyzed TLR genes from the genomes and transcriptomes of 102 amphibian species to understand their evolutionary patterns. Results showed that TLR genes can be divided into seven subfamilies, with TLR4 subfamily found only in Anura. Purification selection played a leading role in amphibian TLR evolution, and different domains had different evolution rates. Positive selection patterns and single-nucleotide polymorphisms were observed in TLR genes.
INTERNATIONAL JOURNAL OF BIOLOGICAL MACROMOLECULES
(2022)
Article
Biotechnology & Applied Microbiology
Xiaochuan Sun, Liang Xu, Yan Wang, Rugang Yu, Xianwen Zhu, Xiaobo Luo, Yiqin Gong, Ronghua Wang, Cecilia Limera, Keyun Zhang, Liwang Liu
Article
Biochemistry & Molecular Biology
Liang Xu, Yan Wang, Wei Liu, Jin Wang, Xianwen Zhu, Keyun Zhang, Rugang Yu, Ronghua Wang, Yang Xie, Wei Zhang, Yiqin Gong, Liwang Liu
Article
Multidisciplinary Sciences
Wei Liu, Liang Xu, Yan Wang, Hong Shen, Xianwen Zhu, Keyun Zhang, Yinglong Chen, Rugang Yu, Cecilia Limera, Liwang Liu
SCIENTIFIC REPORTS
(2015)
Article
Biotechnology & Applied Microbiology
Jian Lin, Jing Xia, Keyun Zhang, Qian Yang
Article
Immunology
Jian Lin, Jing Xia, Ya T. Chen, Ke Y. Zhang, Yan Zeng, Qian Yang
Article
Microbiology
Jian Lin, Jing Xia, Chong Z. Tu, Ke Y. Zhang, Yan Zeng, Qian Yang
FRONTIERS IN MICROBIOLOGY
(2017)
Article
Biochemistry & Molecular Biology
Jian Lin, Jing Xia, Tian Zhang, Keyun Zhang, Qian Yang
Article
Biochemistry & Molecular Biology
Keyun Zhang, Weixian Ding, Jie Sun, Bin Zhang, Fujiao Lu, Ren Lai, Yong Zou, Gabriel Yedid
Article
Chemistry, Medicinal
Jie Sun, Wei-Xian Ding, Xiao-Ping Hong, Ke-Yun Zhang, Yong Zou
CHEMISTRY OF NATURAL COMPOUNDS
(2012)
Article
Biochemistry & Molecular Biology
Guoxiang Wang, Ying Wang, Dongying Ma, Huan Liu, Jianxu Li, Keyun Zhang, Xiaolong Yang, Ren Lai, Jingze Liu
MOLECULAR BIOLOGY REPORTS
(2013)
Article
Zoology
Keyun Zhang, Tiffany Baiocchi, Dihong Lu, Dennis Z. Chang, Adler R. Dillman
JOURNAL OF INVERTEBRATE PATHOLOGY
(2019)
Article
Horticulture
Runsheng Ren, Xingping Yang, Jinhua Xu, Keyun Zhang, Man Zhang, Guang Liu, Xiefeng Yao, Lina Lou, Jian Xu, Lingli Zhu, Qian Hou
Summary: This study systematically analyzed the GDSL esterase/lipase gene family in watermelon, revealing their roles in morphological development, response to abiotic stress, pathogen defense, and secondary metabolism, and also established their phylogenetic relationship with GDSL genes in other plants like Arabidopsis. Transcriptome analysis showed that some GDSL genes are responsive to gummy stem blight, indicating their potential involvement in disease stress response pathways.
SCIENTIA HORTICULTURAE
(2021)
Article
Zoology
Guixin Zhou, Huan Yang, Feng Wang, Haoran Bao, Guoxiang Wang, Xianglong Hou, Jian Lin, Gabriel Yedid, Keyun Zhang
JOURNAL OF NEMATOLOGY
(2017)
Article
Zoology
Pengpeng Li, Chensheng Dai, Haoran Bao, Long Chen, Di Gao, Guoxiang Wang, Jin Wang, Hui Wang, Gabriel Yedid, Keyun Zhang
JOURNAL OF NEMATOLOGY
(2015)
Article
Zoology
Ke Y. Zhang, Xiu H. Liu, Jin Tan, Ying Wang, Lei Qiao, Gabriel Yedid, Chen S. Dai, Ru L. Qiu, Xiu W. Yan, Hao W. Tan, Zhen Y. Su, Ren Lai, Guo F. Gao
JOURNAL OF NEMATOLOGY
(2012)