Article
Biochemistry & Molecular Biology
Yuan Hu, Chenyang Qi, Jiaoyu Shi, Weiqiang Tan, Zhonghua Zhao, Yanyong Xu, Huijuan Wu, Zhigang Zhang
Summary: UCHL1 deficiency leads to severe kidney damage and proteinuria in podocytes, which is associated with ER stress, protein accumulation, and cell apoptosis. UCHL1 may be a potential target for preventing non-immune complex-mediated glomerulopathy.
CELLULAR AND MOLECULAR LIFE SCIENCES
(2023)
Review
Plant Sciences
Runbang Luo, Kun Yang, Wei Xiao
Summary: This article provides a comprehensive review of recent advances in plant deubiquitinases, with a focus on their biochemical activities and biological functions.
PLANT CELL REPORTS
(2023)
Review
Biochemistry & Molecular Biology
Liuqin Zhang, Yifan Guo, Yujie Zhang, Yuxin Li, Yan Pei, Mi Zhang
Summary: The action of auxin largely depends on the establishment of auxin concentration gradient within plant organs, where PIN-mediated directional auxin movement plays an important role. Recent studies have highlighted the importance of PIN degradation in reestablishing auxin gradient. This review summarizes the current understanding on controlling PIN degradation and discusses the possible mechanisms.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Kun Gao, Qing Shi, Ye Gu, Wanqi Yang, Yuanlong He, Zeheng Lv, Yan Ding, Wenxin Cao, Chenji Wang, Xiaoping Wan
Summary: Blockade of PD-1/PD-L1 has shown promise in cancer immunotherapy. This study reveals the role of SPOP gene mutations in regulating PD-L1 expression in endometrial cancer, shedding light on tumor immune evasion mechanisms.
CELL DEATH AND DIFFERENTIATION
(2023)
Article
Chemistry, Multidisciplinary
Anna Sankiewicz, Tomasz Guszcz, Ewa Gorodkiewicz
Summary: The study investigates the relationship between the ubiquitin-proteasome system and transitional cell carcinoma, using SPRi-based biosensors to detect 20S proteasome and UCH-L1 in body fluids of TCC patients. The concentrations of both proteins were found to be significantly elevated in TCC patients compared to healthy subjects, suggesting their potential as cancer biomarkers.
APPLIED SCIENCES-BASEL
(2021)
Article
Pediatrics
Shuying Zeng, Yubo Huang, Tao Zhong, Tao Huang, Xianyan Dong, Huadong Zhu, Fulian Ouyang
Summary: This study found that the expression levels of serum UCH-L1 were significantly elevated in newborns with HIE, correlated with the severity of brain injury, and negatively associated with the development quotient, intelligence index, and NBNA score of newborns.
TRANSLATIONAL PEDIATRICS
(2021)
Article
Biochemistry & Molecular Biology
Han Zhang, Hui Chen, Shutao Yin, Lihong Fan, Caiwei Jin, Chong Zhao, Hongbo Hu
Summary: In this study, it was demonstrated for the first time that omega-3 polyunsaturated fatty acid docosahexaenoic acid can reverse PD-L1-mediated immune suppression by reducing the expression of PD-L1 in cancer cells. The study also showed that DHA inhibits fatty acid synthase and palmitoyltransferase, promoting the degradation of PD-L1.
JOURNAL OF NUTRITIONAL BIOCHEMISTRY
(2023)
Article
Oncology
Macarena Quiroga, Andrea Rodriguez-Alonso, Gloria Alfonsin, Juan Jose Escuder Rodriguez, Sara M. Breijo, Venancio Chantada, Angelica Figueroa
Summary: This review discusses the fundamental role of E3 ubiquitin ligases in controlling cancer stem cells and highlights their potential as effective therapeutic targets for anticancer drug development. Emerging evidence suggests that protein degradation controlled by E3 ubiquitin ligases plays a crucial role in the self-renewal, maintenance, and differentiation of cancer stem cells.
Review
Plant Sciences
Siti Nor Akmar Abdullah, Azzreena Mohamad Azzeme, Kobra Yousefi
Summary: This paper reviews the mechanisms of plant response to cold stress, including transcription factor regulation, early and late responses, and long-term memory reprogramming. The regulatory network can be divided into CBF-dependent and independent pathways. In addition, post-transcriptional regulation by miRNAs, control during ribosomal translation, and post-translational regulation have important effects on the cellular abundance of key regulatory factors.
FRONTIERS IN PLANT SCIENCE
(2022)
Article
Multidisciplinary Sciences
Zhibin Lin, Peijun Yang, Yufeng Hu, Hao Xu, Juanli Duan, Fei He, Kefeng Dou, Lin Wang
Summary: This study reveals the important role of RNF13 protein in NAFLD progression, which can alleviate NAFLD phenotypes by facilitating TRIM29-mediated degradation of STING.
NATURE COMMUNICATIONS
(2023)
Article
Biochemistry & Molecular Biology
Cuihong Jiang, Lili He, Shuai Xiao, Wenqiong Wu, Qi Zhao, Feng Liu
Summary: This study suggests that RNF125 plays a role in inhibiting immune escape in HNSCC by promoting the ubiquitinational degradation of PD-L1. Overexpression of RNF125 inhibits proliferation, migration, and invasion of HNSCC cells, while increasing proliferation of CD8(+) T cells and elevating levels of IL-2 and TNF-alpha. RNF125 also suppresses tumorigenesis and tumor growth in vivo.
MOLECULAR BIOTECHNOLOGY
(2023)
Article
Urology & Nephrology
Aude Dorison, Irene Ghobrial, Alison Graham, Thanushi Peiris, Thomas A. A. Forbes, Michael See, Mithun Das, Moin A. A. Saleem, Catherine Quinlan, Kynan T. T. Lawlor, Mirana Ramialison, Sara E. E. Howden, Melissa H. H. Little
Summary: NPHS2 variants are the most common cause of steroid-resistant nephrotic syndrome in children. This study used induced pluripotent stem cell lines to investigate the effects of NPHS2 variants on the expression and subcellular localization of PODOCIN protein, and found that different variants resulted in distinct intracellular localization of PODOCIN, providing insight into the pathobiology of NPHS2 variants.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Pediatrics
Mallory L. Downie, Sanjana Gupta, Melanie M. Y. Chan, Omid Sadeghi-Alavijeh, Jingjing Cao, Rulan S. Parekh, Carmen Bugarin Diz, Agnieszka Bierzynska, Adam P. Levine, Ruth J. Pepper, Horia Stanescu, Moin A. Saleem, Robert Kleta, Detlef Bockenhauer, Ania B. Koziell, Daniel P. Gale
Summary: A genetic risk score can help classify patients with INS presenting in different ways when monogenic causes are excluded.
PEDIATRIC NEPHROLOGY
(2023)
Article
Urology & Nephrology
David Pitcher, Fiona Braddon, Bruce Hendry, Alex Mercer, Kate Osmaston, Moin A. A. Saleem, Retha Steenkamp, Katie Wong, A. Neil Turner, Kaijun Wang, Daniel P. P. Gale, Jonathan Barratt
Summary: Background IgA nephropathy can progress to kidney failure, and risk assessment soon after diagnosis has advantages both for clinical management and the development of new therapeutics. We present relationships among proteinuria, eGFR slope, and lifetime risks for kidney failure. The study found that almost all patients were at risk of progression to kidney failure within their expected lifetime unless a rate of eGFR loss <= 1 ml/min per 1.73 m(2) per year was maintained.
CLINICAL JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Cell Biology
Tomasz Kulesza, Marlena Typiak, Patrycja Rachubik, Dorota Rogacka, Irena Audzeyenka, Moin A. Saleem, Agnieszka Piwkowska
Summary: Podocytes play a crucial role in blood filtration, and their proper function depends on efficient insulin responsiveness. Insulin resistance of podocytes, characterized by a reduced sensitivity to insulin, is the earliest mechanism leading to microalbuminuria in metabolic syndrome and diabetic nephropathy. This resistance is mediated by NPP1, an enzyme involved in phosphate homeostasis regulation, which inhibits downstream cellular signaling by binding to the insulin receptor. Hyperglycemic conditions also affect Pit 1, a protein involved in phosphate balance, and our study found an interaction between NPP1 and Pit 1 after stimulation with insulin. Downregulation of the SLC20A1 gene, encoding Pit 1, resulted in insulin resistance in podocytes, indicating that Pit 1 may be a key factor in NPP1-mediated insulin signaling inhibition.
JOURNAL OF CELLULAR PHYSIOLOGY
(2023)
Article
Transplantation
Maarten W. Taal, Bethany Lucas, Paul Roderick, Paul Cockwell, David C. Wheeler, Moin A. Saleem, Simon D. S. Fraser, Rosamonde E. Banks, Tim Johnson, Lorna J. Hale, Uwe Andag, Philipp Skroblin, Michaela Bayerlova, Robert Unwin, Nicolas Vuilleumier, Rodolphe Dusaulcy, Fiona Robertson, Elizabeth Colby, David Pitcher, Fiona Braddon, Melissa Benavente, Elaine Davies, Michael Nation, Philip A. Kalra
Summary: The NURTuRE-CKD cohort is a prospective study that aims to investigate risk factors for adverse outcomes in patients with chronic kidney disease (CKD). The study found that a majority of participants were in high-risk CKD categories, with the primary renal diagnosis being unknown cause. Older participants with lower estimated glomerular filtration rate had higher blood pressure and were less likely to be treated with renin-angiotensin system inhibitors, but were more likely to receive a statin.
NEPHROLOGY DIALYSIS TRANSPLANTATION
(2023)
Review
Pediatrics
Samantha Hayward, Kevon Parmesar, Moin A. Saleem
Summary: Nephrotic syndrome (NS) is a clinical triad of hypoalbuminemia, high proteinuria, and edema, with various underlying drivers. The existence of circulating factor disease (CFD) as a driver of NS has been proven by disease recurrence in some transplant patients and laboratory work. Several circulating factors, including protease components and immune components, have been proposed and studied. Promising work is being done to assess CFD risk pre-transplantation through the analysis of 'multi-omic' bioinformatic data.
PEDIATRIC NEPHROLOGY
(2023)
Article
Biochemical Research Methods
Carlos Ramirez R. Medina, Ibrahim Ali, Ivona Baricevic-Jones, Aghogho Odudu, Moin A. Saleem, Anthony D. Whetton, Philip A. Kalra, Nophar Geifman
Summary: Using SWATH-MS analysis, we identified 25 biomarkers associated with progression in chronic kidney disease (CKD), which are related to subtype classification and disease-relevant pathways. These findings provide important leads for prevention and treatment of CKD.
CLINICAL PROTEOMICS
(2023)
Article
Cell Biology
Rim Bouslama, Vincent Dumont, Sonja Lindfors, Lassi Paavolainen, Jukka Tienari, Harry Nisen, Tuomas Mirtti, Moin A. Saleem, Daniel Gordin, Per-Henrik Groop, Shiro Suetsugu, Sanna Lehtonen
Summary: Changes in the podocytes, the glomerular epithelial cells, can lead to kidney dysfunction. The phosphorylation of PACSIN2 at serine 313 (S313) is increased in the glomeruli of rats with diabetic kidney disease, and this phosphorylation is associated with kidney dysfunction and increased free fatty acids. PACSIN2 phosphorylation is a dynamic process that regulates cell morphology and cytoskeletal arrangement in cooperation with N-WASP. The phosphorylation of PACSIN2 at S313 is important for regulating cytoskeletal reorganization.
Review
Biochemistry & Molecular Biology
Samantha Hayward, Kevon Parmesar, Gavin I. Welsh, Matthew Suderman, Moin A. Saleem
Summary: A small subset of people with nephrotic syndrome (NS) have genetically driven disease, while the mechanisms for the remaining majority are unknown. Epigenetic marks have potential as biomarkers and therapeutic targets. The majority of published human studies on epigenetic mechanisms in NS have examined micro-RNAs (miRNAs), and there are some promising concordant results. However, the study quality is low and larger clinical studies focusing on DNA methylation and histone modifications are needed to address this critical gap in the literature.
Article
Biochemistry & Molecular Biology
Maria D. Sanchez-Nino, Maria I. Ceballos, Sol Carriazo, Aranzazu Pintor-Chocano, Ana B. Sanz, Moin A. Saleem, Alberto Ortiz
Summary: The interaction between diabetes and Fabry disease may increase the severity of kidney damage by modulating the Gb3 synthesis pathway and downregulating kidney protective genes.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Urology & Nephrology
Masaomi Nangaku, A. Richard Kitching, Peter Boor, Alessia Fornoni, Jurgen Floege, P. Toby Coates, Jonathan Himmelfarb, Rachel Lennon, Hans-Joachim Anders, Benjamin D. Humphreys, Fergus J. Caskey, Agnes B. Fogo
Summary: The International Society of Nephrology organized the TRANSFORM meeting to provide guidance on translational animal studies for new drug development in kidney disease. The meeting covered various themes such as disease model selection, pharmacokinetics, interventions, choice of animal, statistical power, organoids and organ-on-a-chip models, and reporting of results. These recommendations aim to accelerate the development of new drugs for efficacious diseases.
KIDNEY INTERNATIONAL
(2023)
Article
Cell Biology
Wen Y. Ding, Valeryia Kuzmuk, Sarah Hunter, Abigail Lay, Bryony Hayes, Matthew Beesley, Ruth Rollason, Jennifer A. Hurcombe, Fern Barrington, Catrin Masson, William Cathery, Carl May, Jack Tuffin, Timothy Roberts, Geraldine Mollet, Colin J. Chu, Jenny McIntosh, Richard J. Coward, Corinne Antignac, Amit Nathwani, Gavin I. Welsh, Moin A. Saleem
Summary: Gene therapy using AAV vector has successfully addressed the challenges of kidney diseases, showing particular success in targeting monogenic diseases in other organs. By utilizing AAV-LK03 and AAV 2/9, gene therapy has been able to rescue genetic defects and improve kidney disease in human and mouse models, establishing the podocyte as a viable target for gene therapy.
SCIENCE TRANSLATIONAL MEDICINE
(2023)
Article
Medicine, Research & Experimental
Emily E. Bowen, Jennifer A. Hurcombe, Fern Barrington, Lindsay S. Keir, Louise K. Farmer, Matthew D. Wherlock, Carolina G. Ortiz-Sandoval, Valentina Brun, Arlette Bohorquez-Hernandez, Daniel Diatlov, Niyousha Rostam-Shirazi, Sara Wells, Michelle Stewart, Lydia Teboul, Abigail C. Lay, Matthew J. Butler, Robert J. P. Pope, Eva M. S. Larkai, B. Paul Morgan, John Moppett, Simon C. Satchell, Gavin I. Welsh, Patrick D. Walker, Christoph Licht, Moin Saleem, Richard J. M. Coward
Summary: This study elucidates the pathogenesis of STEC-HUS and suggests the early use of terminal complement pathway inhibitors as a treatment option.
Article
Urology & Nephrology
Howard Trachtman, Rosanna Coppo, Moin Saleem, Alex Mercer, Radko Komers
JOURNAL OF NEPHROLOGY
(2023)
Article
Multidisciplinary Sciences
Irena Audzeyenka, Maria Szrejder, Dorota Rogacka, Stefan Angielski, Moin A. A. Saleem, Agnieszka Piwkowska
Summary: This study found that human podocytes express the BAIBA receptor MRGPRD, and stimulation with BAIBA can improve the respiratory parameters and promote mitochondrial dynamics in podocytes, indicating the importance of mitochondrial function in maintaining the renal filtration barrier.
SCIENTIFIC REPORTS
(2023)