Article
Biochemistry & Molecular Biology
Jing Zheng, Yan Xie, Toru Yoshitomi, Naoki Kawazoe, Yingnan Yang, Guoping Chen
Summary: This study investigated different microenvironmental conditions for the controlled proliferation and chondrogenic differentiation of human bone-marrow-derived mesenchymal stem cells (hMSCs). The results showed that collagen sponges promoted cell proliferation and chondrogenic differentiation. Combining collagen sponges with collagen hydrogels and induction factors had the highest promotive effect on chondrogenic differentiation. These findings provide useful information for stem cell chondrogenic differentiation and cartilage tissue engineering.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell & Tissue Engineering
Daniele Zuncheddu, Elena Della Bella, Dalila Petta, Cecilia Bartschi, Sonja Hackel, Moritz C. Deml, Martin J. Stoddart, Sibylle Grad, Valentina Basoli
Summary: This study investigates the effects of glucose and fructose on the chondrogenic differentiation of BMSCs. The results show that fructose may enhance chondrogenic differentiation, while decreasing glucose concentration may decrease differentiation yield. The study also reveals the expression and regulation of GLUT3 and GLUT5 in BMSCs during chondrogenesis. The findings provide valuable insights into the sugar metabolism of BMSCs during cartilage formation.
STEM CELL RESEARCH & THERAPY
(2022)
Article
Cell & Tissue Engineering
Akihiro Yamashita, Hiroyuki Yoshitomi, Shunsuke Kihara, Junya Toguchida, Noriyuki Tsumaki
Summary: This study demonstrated that hiPSCs could form cartilage on Matrigel but not on laminin 511-E8, with differences observed in cell morphology and YAP activity. Transient knockdown of YAP around the start of chondrogenic differentiation successfully addressed the issue of cartilage formation from hiPSCs on laminin 511-E8.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Oncology
Shiqi Xiang, Zixuan Lin, Meagan J. J. Makarcyzk, Kanyakorn Riewruja, Yiqian Zhang, Xiurui Zhang, Zhong Li, Karen L. Clark, Eileen Li, Silvia Liu, Tingjun Hao, Madalyn R. R. Fritch, Peter G. G. Alexander, Hang Lin
Summary: This study elucidates the difference between hiMPCs and hMSCs in response to TGF beta and presents a robust method for obtaining low hypertrophy hiMPCs-derived cartilage. The results also indicate the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Biochemistry & Molecular Biology
Zhongrun Yuan, Sa Liu, Wenjing Song, Ying Liu, Gangyuan Bi, Renjian Xie, Li Ren
Summary: Galactose has been found to promote chondrogenesis and cartilage matrix formation, which suggests its potential for cartilage repair and regeneration.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Engineering, Biomedical
Jiabin Zhang, Ming Zhang, Rongcai Lin, Yuguang Du, Liming Wang, Qingqiang Yao, Andrew Zannettino, Hu Zhang
Summary: Stem cell therapy using magnetic scaffolds has been developed to treat osteoarthritis. The magnetic scaffolds enhance chondrogenesis of mesenchymal stem cells through mechanical stimulation and magnetic field effects. The fiber topography of the scaffolds can be tuned with different coatings on magnetic nanoparticles, which selectively up-regulate chondrogenesis or osteogenesis-related genes.
Article
Biochemistry & Molecular Biology
Marija Mazor, Eric Lespessailles, Thomas M. Best, Mazen Ali, Hechmi Toumi
Summary: Recent research has shown that cells isolated from osteoarthritic cartilage have the potential to form hyaline-like cartilage tissue. In this study, the researchers examined the marker expression and chondrogenetic potential of cells from mild, moderate, and severe osteoarthritic tissue. They found that only cells from moderate osteoarthritis were able to form hyaline cartilage-like tissue, while cells from severe and mild osteoarthritis did not. These findings have implications for understanding the repair capacity of articular cartilage in osteoarthritis and suggest that these progenitor cells may be a candidate for cartilage repair.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Cell Biology
Denise Zujur, Ziadoon Al-Akashi, Anna Nakamura, Chengzhu Zhao, Kazuma Takahashi, Shizuka Aritomi, William Theoputra, Daisuke Kamiya, Koichi Nakayama, Makoto Ikeya
Summary: We developed a stepwise differentiation method to generate matrix-rich cartilage spheroids from induced pluripotent stem cell-derived mesenchymal stem/stromal cells (iMSCs) via the induction of neural crest cells under xeno-free conditions. By using a thienoindazole derivative, TD-198946, we achieved enhanced chondrogenesis in iMSCs. Our findings provide a novel cell source for stem cell-based cartilage repair and offer the potential for biofabrication of larger cartilage tissues using chondrogenic spheroids and technologies such as Kenzan Bioprinting.
FRONTIERS IN CELL AND DEVELOPMENTAL BIOLOGY
(2023)
Article
Biotechnology & Applied Microbiology
Chantal Voskamp, Wendy J. L. M. Koevoet, Gerjo J. V. M. Van Osch, Roberto Narcisi
Summary: This study investigated the impact of senescence and the senescence-associated secretory phenotype (SASP) on chondrogenic differentiation of MSCs. Inducing senescence in cells during expansion or early differentiation significantly reduced cartilage matrix formation. However, inducing senescence at a later stage of differentiation did not significantly affect chondrogenesis.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2023)
Article
Pharmacology & Pharmacy
Natalia Carballo-Pedrares, Clara Sanjurjo-Rodriguez, Jose Senaris, Silvia Diaz-Prado, Ana Rey-Rico
Summary: This study utilized new non-viral carriers based on niosomes to promote effective chondrogenesis of human mesenchymal stem cells, showing promise for gene therapy approaches focused on cartilage repair.
Article
Biotechnology & Applied Microbiology
Yuxiang Ge, Yixuan Li, Zixu Wang, Lan Li, Huajian Teng, Qing Jiang
Summary: The study found that early application of dynamic compression had a suppressive effect on the expression of chondrocyte-specific markers, while late application of dynamic compression promoted cartilage matrix gene expression and suppressed hypertrophic development. Dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in maintaining the cartilage phenotype.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Nanoscience & Nanotechnology
Aman Mahajan, Akhilesh Singh, Dipak Datta, Dhirendra S. Katti
Summary: A triple-network injectable hydrogel system was developed, which increased stiffness and contraction and promoted chondrogenesis by mimicking key steps in cartilage development, showing great potential for cartilage repair.
ACS APPLIED MATERIALS & INTERFACES
(2022)
Article
Immunology
Cristina Duarte-Olivenza, Juan Antonio Montero, Carlos Ignacio Lorda-Diez
Summary: The study revealed the dual and opposite effects of berberine on the growth and differentiation of skeletal progenitors, showing an inhibitory or promoting effect on chondrogenesis depending on the stage of culture. Furthermore, berberine may be involved in cartilage differentiation through transcriptional influence on DNA methyltransferase genes, suggesting the potential role of epigenetic factors in its effects. These findings have important implications for the clinical use of berberine in the treatment of cartilage degenerative diseases.
JOURNAL OF INFLAMMATION RESEARCH
(2021)
Article
Cell & Tissue Engineering
Mira Hammad, Alexis Veyssiere, Sylvain Leclercq, Vincent Patron, Catherine Bauge, Karim Boumediene
Summary: Ear cartilage malformations are challenging problems in reconstructive surgery, and current treatment methods have limitations. Tissue engineering using a natural scaffold and cells from different origins shows promise in regenerating cartilage for future clinical applications. This study investigates the viability and differentiation of various cell types on a natural scaffold, and highlights the superior performance of auricular perichondrial cells and the effect of hypoxia on these cells.
INTERNATIONAL JOURNAL OF STEM CELLS
(2023)
Article
Biochemistry & Molecular Biology
Christopher A. Smith, Paul A. Humphreys, Nicola Bates, Mark A. Naven, Stuart A. Cain, Mona Dvir-Ginzberg, Susan J. Kimber
Summary: The activation of SIRT1 has a positive impact on the expression of main extracellular matrix (ECM) proteins during human cartilage development, but it also alters ECM composition and suppresses the content of glycosaminoglycans (GAGs). The activation of SIRT1 is associated with increased expression of ECM genes and chondrogenic transcription factors, as well as an interaction with ARID5B. However, the activation of SIRT1 also leads to a decrease in GAGs and down regulation of genes responsible for GAG chain initiation/elongation.