Article
Endocrinology & Metabolism
Maysa S. S. Campos, Jose B. Volpon, Joao Paulo B. Ximenez, Ana Paula Franttini, Christopher E. Dalloul, Manoel D. Sousa-Neto, Raquel A. A. Silva, Melissa A. A. Kacena, Ariane Zamarioli
Summary: This study investigated the effects of vibration therapy on fracture healing in diabetic and non-diabetic rats. The results showed that diabetes had detrimental effects on bone healing, but vibration therapy was effective at counteracting these effects in diabetic rats. Vibration therapy promoted osteogenic and chondrogenic cell proliferation, improved bone healing structure and quantity, and restored serum levels of certain factors involved in bone repair.
FRONTIERS IN ENDOCRINOLOGY
(2022)
Article
Endocrinology & Metabolism
Melanie Haffner-Luntzer, Deniz Ragipoglu, Mubashir Ahmad, Astrid Schoppa, Lena Steppe, Verena Fischer, Julia Luther, Timur Yorgan, Ernesto Bockamp, Michael Amling, Thorsten Schinke, Anita Ignatius
Summary: Despite improvements in fracture care, a percentage of fractures still heal poorly. The study investigated the potential of Wnt1 as a molecule to improve bone fracture healing. The results showed that Wnt1 promotes bone formation during fracture healing through YAP/BMP signaling. This suggests that Wnt1 could be used as a therapeutic agent for orthopedic complications.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Multidisciplinary Sciences
Esther Wehrle, Graeme R. Paul, Duncan C. Tourolle Ne Betts, Gisela A. Kuhn, Ralph Muller
Summary: The study demonstrated the significant impact of individualized cyclic mechanical loading on the remodelling phase of fracture healing in a mouse femur defect model. Changes in dynamic parameters led to increased formation of mineralized callus. The maintenance of callus remodelling was associated with distinct effects on molecular targets, showing the potential for further understanding the mechano-molecular regulation of fracture healing phases.
SCIENTIFIC REPORTS
(2021)
Article
Biology
Dominik Saul, David G. Monroe, Jennifer L. Rowsey, Robyn Laura Kosinsky, Stephanie J. Vos, Madison L. Doolittle, Joshua N. Farr, Sundeep Khosla
Summary: Senescent cells and senescence-related secretory phenotype markers increase during fracture healing. Intermittent treatment with senolytics reduces senescence markers in the fracture callus and accelerates the time course of fracture healing.
Article
Materials Science, Multidisciplinary
Yan Peng, Huaizhi Liu, Hao Peng, Jiuyang Zhang
Summary: Self-healing materials have long struggled to achieve both high mechanical strength and self-healing ability. This work successfully achieves self-repair in mechanically robust materials, inspired by biological self-healing. The mechanical performance and self-healing behavior of these materials can be precisely controlled through temperature and composition. An artificial bone-tissue system is created with bionic structure, effective self-healing, and high modulus.
Article
Biotechnology & Applied Microbiology
Lena Steppe, Benjamin Thilo Krueger, Miriam Eva Angelica Tschaffon, Verena Fischer, Jan Tuckermann, Anita Ignatius, Melanie Haffner-Luntzer
Summary: Biomechanical stimulation by whole-body low-magnitude high-frequency vibration has shown to have anabolic effects on bone metabolism. Specifically, osteoblast lineage-specific ER alpha plays a critical role in LMHFV-induced effects on fracture healing, with WNT and Cox2/PGE(2) signaling pathways involved in the mechanotransduction process.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Review
Endocrinology & Metabolism
Dominik Saul, Sundeep Khosla
Summary: More than 2.1 million age-related fractures occur in the United States annually, resulting in a significant socioeconomic burden. Age-related deterioration of bone structure is associated with impaired bone healing, which occurs in four stages: inflammation, angiogenesis and cartilage formation, soft callus development, and remodeling. Impaired fracture healing due to aging is related to detrimental changes at the cellular level, and recent studies show that clearing senescent cells enhances fracture repair.
Article
Biochemistry & Molecular Biology
Silvia Concetta Colucci, Cinzia Buccoliero, Lorenzo Sanesi, Mariella Errede, Graziana Colaianni, Tiziana Annese, Mohd Parvez Khan, Roberta Zerlotin, Manuela Dicarlo, Ernestina Schipani, Kenneth M. Kozloff, Maria Grano
Summary: The study found that irisin promotes bone formation and accelerates the fracture healing process, suggesting its potential use as a novel pharmacologic modulator.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Radiology, Nuclear Medicine & Medical Imaging
Andre L. B. D. Cardoso, Eric H. F. F. Frederico, Carlos A. S. Guimaraes, Eliane O. Guedes-Aguiar, Eloa Moreira-Marconi, Laisa L. Paineiras-Domingos, Danubia C. Sa-Caputo, Mario Bernardo-Filho, Nasser R. Asad
Summary: The study investigated the effect of whole-body vibration exercise on bone density in rats through X-ray analysis, revealing an increase in bone density dependent on vibration stimulus frequency, particularly at 10Hz and 20Hz frequencies.
ACADEMIC RADIOLOGY
(2021)
Article
Medicine, General & Internal
Chul-Ho Kim, Dong Hwan Kim, Han-Vit Kang, Won Jun Kim, Minkyu Shin, Ji Wan Kim
Summary: Percutaneous intramedullary nailing is an effective technique for metacarpal fractures, but the fracture site and number of K-wires used can affect the time to achieve bone union.
Article
Biotechnology & Applied Microbiology
Helen Rinderknecht, Andreas K. Nussler, Konrad Steinestel, Tina Histing, Sabrina Ehnert
Summary: Angiogenesis plays a crucial role in the early healing process of bone fractures. This study developed an in vitro model to simulate smoking conditions and investigate the impact of smoking on angiogenesis and fracture repair.
BIOENGINEERING-BASEL
(2022)
Review
Geriatrics & Gerontology
Christel Harijanto, Anthony Lim, Sara Vogrin, Gustavo Duque
Summary: The study assessed the effects of whole-body vibration (WBV) on bone and muscle parameters in older people. The meta-analysis showed that WBV did not have significant synergistic effects on measured bone and muscle outcomes. Further research with larger sample sizes and longer follow-up periods is needed to establish the clinical efficacy of WBV on bone and muscle health in older individuals.
Article
Endocrinology & Metabolism
Lena Steppe, Benjamin Krueger, Miriam Eva Angelica Tschaffon-Mueller, Jan-Moritz Ramge, Astrid Schoppa, Anita Ignatius, Melanie Haffner-Luntzer
Summary: External mechanostimulation induced by LMHFV has different effects on fracture healing in estrogen-competent and estrogen-deficient rodents. ER alpha signaling in osteoblasts is required for these effects, with ligand-dependent and -independent ER alpha signaling playing different roles. The AF-2 domain of the ER alpha receptor is crucial for the negative effects of vibration during bone fracture healing in estrogen-competent mice, suggesting that the osteoanabolic effects of vibration are mediated by ligand-independent ER alpha signaling.
Article
Biology
Kai Sun, Chun Wang, Jianqiu Xiao, Michael D. Brodt, Luorongxin Yuan, Tong Yang, Yael Alippe, Huimin Hu, Dingjun Hao, Yousef Abu-Amer, Matthew J. Silva, Jie Shen, Gabriel Mbalaviele
Summary: Amino-terminal fragments of proteolytically cleaved GSDMs form plasma membrane pores, leading to pyroptosis. GSDMD and GSDME play roles in bone microenvironment and contribute to fracture healing. Both GSDMD and GSDME regulate inflammatory responses through the GSDM-IL-1 axis, promoting bone healing after fracture.
Article
Cell & Tissue Engineering
R. M. Y. Wong, V. M. H. Choy, J. Li, T. K. Li, Y. N. Chim, M. C. M. Li, J. C. Y. Cheng, K-S Leung, S. K-H Chow, W. H. Cheung
Summary: The study showed that LMHFV can enhance fibrinolysis, accelerate fracture healing, and improve mechanical properties. It is a promising intervention for osteoporotic fracture healing that justifies further translation to clinical studies.
BONE & JOINT RESEARCH
(2021)
Article
Orthopedics
Andreas M. Seitz, Janina Leiprecht, Jonas Schwer, Anita Ignatius, Heiko Reichel, Thomas Kappe
Summary: The purpose of this study was to investigate the potential of a doubled semitendinosus (ST) and a single gracilis tendon (GT) lateral meniscus autograft to restore knee joint kinematics and tibiofemoral contact after total lateral meniscectomy (LMM). The results showed that the ST autograft significantly improved knee joint kinematics and restored tibiofemoral contact mechanics, while the GT autograft had limited effectiveness.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Article
Immunology
Melanie Haffner-Luntzer, Birte Weber, Kazuhito Morioka, Ina Lackner, Verena Fischer, Chelsea Bahney, Anita Ignatius, Miriam Kalbitz, Ralph Marcucio, Theodore Miclau
Summary: Clinical and preclinical data suggest accelerated bone fracture healing in subjects with an additional traumatic brain injury (TBI). Mechanistically, altered metabolism and neuro-endocrine regulations have been shown to influence bone formation after combined fracture and TBI, thereby increasing the bone content in the fracture callus. However, the early inflammatory response towards fracture and TBI has not been investigated in detail so far.
FRONTIERS IN IMMUNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Jana Riegger, Helga Joos, Valentin Moehler, Frank Leucht, Katrin Rading, Christian Kubisch, Anita Ignatius, Markus Huber-Lang, Rolf E. Brenner
Summary: The study suggests that the terminal complement complex (TCC) plays a role in the pathogenesis of posttraumatic osteoarthritis (PTOA). However, the absence of TCC may protect against OA progression and have minor effects on the micro-structure of the subchondral bone.
Review
Oncology
Franziska Lioba Breulmann, Luan Phelipe Hatt, Boris Schmitz, Esther Wehrle, Robert Geoff Richards, Elena Della Bella, Martin James Stoddart
Summary: This systematic review aimed to identify common miRNAs involved in fracture healing or non-union fractures. Out of 151 miRNAs, miR-21, miR-140 and miR-214 were the most investigated miRNAs in fracture healing in general, while miR-31-5p, miR-221 and miR-451-5p were identified to be regulated specifically in non-union fractures. Despite heterogeneity between studies, these miRNAs have the potential to serve as biomarkers for non-union fractures.
CLINICAL AND TRANSLATIONAL MEDICINE
(2023)
Article
Endocrinology & Metabolism
Mubashir Ahmad, Nadine Stirmlinger, Irfana Jan, Ulrich Stifel, Sooyeon Lee, Marcel Weingandt, Ulrike Kelp, Juergen Bockmann, Anita Ignatius, Tobias M. Boeckers, Jan Tuckermann
Summary: Mutations in the postsynaptic scaffold protein Shank2 are associated with autism spectrum disorders (ASD) and an increased risk of fractures in affected patients. This study investigates the direct role of Shank2 in regulating bone mass. The findings indicate that Shank2 is expressed in bone and plays a crucial role in osteoblast differentiation and bone formation. The interaction between Shank2 and proteins known from the central nervous system is identified as important for osteoblast differentiation.
Article
Biochemistry & Molecular Biology
Franziska Widmayer, Cornelia Neidlinger-Wilke, Fiona Witz, Jan U. Jansen, Anita Ignatius, Melanie Haffner-Luntzer, Graciosa Q. Teixeira
Summary: Postmenopausal women have an increased risk of intervertebral disc degeneration, possibly due to decreased estrogen levels. This study investigated the effects of 17-beta-estradiol (E2) and low-magnitude, high-frequency vibration (LMHFV) on intervertebral discs (IVDs). The results showed that E2 treatment had minor effects on gene expression, cellularity, and matrix metabolism, while LMHFV alone led to cell loss and increased production of IL-6. However, the combination of E2 and LMHFV had a protective effect against cell loss and decreased IL-6 production.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Biochemistry & Molecular Biology
Verena Fischer, Jasmin Maria Buelow, Benjamin Thilo Krueger, Deniz Ragipoglu, Anna Vikman, Melanie Haffner-Luntzer, Konstantinos Katsoulis-Dimitriou, Anne Dudeck, Anita Ignatius
Summary: Previous studies have shown that an increase in mast cells in the bone marrow is associated with age-related and post-menopausal osteoporosis. However, this study found that mast cell-derived RANKL does not contribute to the development of post-menopausal osteoporosis, suggesting that other factors may be responsible for this condition.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2023)
Article
Endocrinology & Metabolism
Melanie Haffner-Luntzer, Deniz Ragipoglu, Mubashir Ahmad, Astrid Schoppa, Lena Steppe, Verena Fischer, Julia Luther, Timur Yorgan, Ernesto Bockamp, Michael Amling, Thorsten Schinke, Anita Ignatius
Summary: Despite improvements in fracture care, a percentage of fractures still heal poorly. The study investigated the potential of Wnt1 as a molecule to improve bone fracture healing. The results showed that Wnt1 promotes bone formation during fracture healing through YAP/BMP signaling. This suggests that Wnt1 could be used as a therapeutic agent for orthopedic complications.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Review
Endocrinology & Metabolism
Merle Stein, Florent Elefteriou, Bjorn Busse, Imke A. K. Fiedler, Ronald Young Kwon, Eric Farrell, Mubashir Ahmad, Anita Ignatius, Liam Grover, Liesbet Geris, Jan Tuckermann
Summary: Major achievements in bone research have relied on animal models and in vitro systems. However, the ethical debate surrounding animal experimentation and advancements in in vitro and in silico techniques have challenged their use. This review provides an overview of the strengths and limitations of animal models, as well as in vitro and in silico technologies, in skeletal research. The authors propose that a combination of the right animal model and state-of-the-art technology is essential for advancing bone research.
JOURNAL OF BONE AND MINERAL RESEARCH
(2023)
Article
Engineering, Biomedical
Lena Schroeter, Friederike Kaiser, Anna-Lena Preissler, Philipp Wohlfahrt, Oliver Kueppers, Uwe Gbureck, Anita Ignatius
Summary: To address the challenges of time-consuming and error-prone preparation of hydroxyapatite (HA) cements for bone defect treatment, researchers have developed a prefabricated magnesium phosphate cement paste based on glycerol. This ready-to-use paste can be directly applied during surgery and shows a promising degradation rate and support for bone regeneration.
ADVANCED HEALTHCARE MATERIALS
(2023)
Article
Orthopedics
Matthias Sukopp, Maoz Shemesh, Elena Pruech, Eran Linder-Ganz, Scott Hacker, Vincenzo Condello, Jonas Schwer, Anita Ignatius, Lutz Duerselen, Andreas Martin Seitz
Summary: The purpose of this in-vitro study was to examine the kinematics of an artificial, free-floating medial meniscus replacement device under dynamic loading situations and different knee joint states. A dynamic knee simulator was used to perform dynamic loading exercises on left human cadaveric knee joints to investigate the knee joint and implant kinematics. The results showed that the non-anchored free-floating device remains within the medial knee joint gap under challenging dynamic loading situations without indicating any luxation tendencies.
JOURNAL OF EXPERIMENTAL ORTHOPAEDICS
(2023)
Article
Endocrinology & Metabolism
Lena Steppe, Benjamin Krueger, Miriam Eva Angelica Tschaffon-Mueller, Jan-Moritz Ramge, Astrid Schoppa, Anita Ignatius, Melanie Haffner-Luntzer
Summary: External mechanostimulation induced by LMHFV has different effects on fracture healing in estrogen-competent and estrogen-deficient rodents. ER alpha signaling in osteoblasts is required for these effects, with ligand-dependent and -independent ER alpha signaling playing different roles. The AF-2 domain of the ER alpha receptor is crucial for the negative effects of vibration during bone fracture healing in estrogen-competent mice, suggesting that the osteoanabolic effects of vibration are mediated by ligand-independent ER alpha signaling.
Article
Orthopedics
Luisa de Roy, Kerstin Eichhorn, Martin Faschingbauer, Klaus Schlickenrieder, Anita Ignatius, Andreas Martin Seitz
Summary: The purpose of this in vitro study was to investigate the effect of hyaluronic acid supplementation on knee joint friction during osteoarthritis progression. The results showed that knee joint friction does not increase with degeneration, and hyaluronic acid supplementation does not lead to an initial decrease in friction.
KNEE SURGERY SPORTS TRAUMATOLOGY ARTHROSCOPY
(2023)
Meeting Abstract
Dermatology
P. Maity, K. Singh, L. Krug, A. Koroma, W. Bloch, S. Kochanek, M. Wlaschek, M. Schorpp-Kistner, P. Angel, A. Ignatius, H. Geiger, K. Scharffetter-Kochanek
EXPERIMENTAL DERMATOLOGY
(2023)
Review
Biochemistry & Molecular Biology
Jana Riegger, Astrid Schoppa, Leonie Ruths, Melanie Haffner-Luntzer, Anita Ignatius
Summary: This article discusses oxidative stress as a major driver of pathophysiological processes in cartilage and bone, including aging, misdirected differentiation, cell death, mitochondrial dysfunction, and impaired mitophagy. It also elaborates on cellular defense mechanisms and therapeutic strategies to improve cell and tissue protection.
CELLULAR & MOLECULAR BIOLOGY LETTERS
(2023)
