Article
Biotechnology & Applied Microbiology
Yuxiang Ge, Yixuan Li, Zixu Wang, Lan Li, Huajian Teng, Qing Jiang
Summary: The study found that early application of dynamic compression had a suppressive effect on the expression of chondrocyte-specific markers, while late application of dynamic compression promoted cartilage matrix gene expression and suppressed hypertrophic development. Dynamic mechanical compression loading not only promotes chondrogenic differentiation of SMSCs, but also plays a vital role in maintaining the cartilage phenotype.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Oncology
Shiqi Xiang, Zixuan Lin, Meagan J. J. Makarcyzk, Kanyakorn Riewruja, Yiqian Zhang, Xiurui Zhang, Zhong Li, Karen L. Clark, Eileen Li, Silvia Liu, Tingjun Hao, Madalyn R. R. Fritch, Peter G. G. Alexander, Hang Lin
Summary: This study elucidates the difference between hiMPCs and hMSCs in response to TGF beta and presents a robust method for obtaining low hypertrophy hiMPCs-derived cartilage. The results also indicate the importance of activating both Smad2/3 and Smad1/5 in the initiation of chondrogenesis.
CLINICAL AND TRANSLATIONAL MEDICINE
(2022)
Article
Orthopedics
Khay-Yong Saw, Adam W. Anz, Reza Ching-Soong Ng, Caroline Siew-Yoke Jee, Soo Fin Low, Christopher Dorvault, Kevin B. Johnson
Summary: The study confirmed that arthroscopic marrow stimulation with subchondral drilling followed by postoperative intra-articular injections of autologous PBSCs plus HA significantly improved knee joint function and reduced pain, with good safety profile.
ARTHROSCOPY-THE JOURNAL OF ARTHROSCOPIC AND RELATED SURGERY
(2021)
Article
Orthopedics
Lukas Zak, Anne Kleiner, Christian Albrecht, Brigitte Tichy, Silke Aldrian
Summary: The 2-year follow-up of patients who received third-generation ACI using the Igor scaffold at the knee joint showed improvements in clinical and radiological outcomes, with a decrease in pain, an increase in activity, and the majority of patients reporting good results.
ORTHOPAEDIC JOURNAL OF SPORTS MEDICINE
(2021)
Article
Cell & Tissue Engineering
Akihiro Yamashita, Hiroyuki Yoshitomi, Shunsuke Kihara, Junya Toguchida, Noriyuki Tsumaki
Summary: This study demonstrated that hiPSCs could form cartilage on Matrigel but not on laminin 511-E8, with differences observed in cell morphology and YAP activity. Transient knockdown of YAP around the start of chondrogenic differentiation successfully addressed the issue of cartilage formation from hiPSCs on laminin 511-E8.
STEM CELLS TRANSLATIONAL MEDICINE
(2021)
Article
Cell Biology
Felicia A. M. Klampfleuthner, Benedict Lotz, Tobias Renkawitz, Wiltrud Richter, Solvig Diederichs
Summary: PI3K/AKT signaling plays an important role in the speed of chondrogenesis in human mesenchymal stromal cells (MSCs), but not in hypertrophic degeneration. This study provides valuable insights for improving MSC chondrogenesis and generating high-quality cartilage.
Article
Cell & Tissue Engineering
H. Binder, L. Hoffman, L. Zak, T. Tiefenboeck, S. Aldrian, C. Albrecht
Summary: This retrospective study aimed to compare short-term clinical outcomes among four different types of matrix-associated autologous chondrocyte transplantation (MACT) for full-thickness chondral lesions of the tibiofemoral joint. The study found that two years postoperatively, there were no significant differences in terms of outcomes, demonstrating that MACT resulted in good clinical improvement regardless of the implants used for localized tibiofemoral defects.
BONE & JOINT RESEARCH
(2021)
Article
Critical Care Medicine
Jose Marchan, Olga Wittig, Dylana Diaz-Solano, Marcos Gomez, Jose E. Cardier
Summary: This study investigated the capacity of mesenchymal stromal cells (MSCs) to promote chondrogenesis from chondrocytes or chondrons in 2D and 3D coculture systems. The results demonstrated that MSCs can promote the proliferation of functional chondrocytes, enhance the expression of cartilage genes, and increase GAG production. These findings provide a potential treatment approach for cartilage repair using chondrogenic constructs based on MSCs and chondrocytes.
INJURY-INTERNATIONAL JOURNAL OF THE CARE OF THE INJURED
(2022)
Review
Chemistry, Multidisciplinary
Nilotpal Majumder, Sourabh Ghosh
Summary: Successful recapitulation of the anatomical microarchitecture and biomechanics of the native articular cartilage under in vitro culture conditions remains challenging. The major obstacle is maintaining the stable chondrogenic phenotype, which is often transformed into chondrocyte hypertrophy. This review summarizes the underlying causes of hypertrophic phenotype development in chondrocytes under in vitro chondrogenic conditions, highlighting the role of cell type, culture conditions, and microenvironment.
ADVANCED FUNCTIONAL MATERIALS
(2023)
Article
Biochemistry & Molecular Biology
Yikai Wang, Zibo Liu, Chuqiao Pan, Yi Zheng, Yahong Chen, Xiang Lian, Yu Jiang, Chuhsin Chen, Ke Xue, Yuanyuan Zhang, Peng Xu, Kai Liu
Summary: This study found that extracellular vesicles derived from chondrocytes can enhance the chondrogenesis of adipose-derived stem cells in vitro, and low-intensity ultrasound stimulation can increase the secretion of these vesicles. Additionally, ultrasound-driven healing enhances the quantity and quality of the extracellular vesicles by enriching cartilage-regeneration-associated miRNAs.
Article
Engineering, Biomedical
Jinrui Zhang, Bo Li, Jianlin Zuo, Rui Gu, Bin Liu, Chao Ma, Jingjing Li, Kai Liu
Summary: The development of a novel protein adhesive with strong tissue adhesion and chondrogenic capabilities for cartilage repair has been reported in this study. This adhesive, fabricated by the electrostatic combination of chondroitin sulfate (CS) and positively charged elastin-like protein, showed robust adhesion properties on different tissue substrates and promoted cartilage tissue integration. It exhibited beneficial healing effects in a rat model of cartilage defect, making it a promising candidate for translational application in the field of cartilage repair.
ADVANCED HEALTHCARE MATERIALS
(2021)
Article
Orthopedics
Raquel Vayas, Ricardo Reyes, Maria Rosa Arnau, Carmen Evora, Araceli Delgado
Summary: The study evaluated a minimally invasive approach against conventional MFX for repairing chondral defect in rabbits using an injectable scaffold with BMP-2. Results showed that treatments with MSCs or BMP-2 efficiently repaired the chondral lesion with less invasiveness than MFX.
Review
Cell Biology
Inga Urlic, Alan Ivkovic
Summary: Cell-based therapy is a promising strategy for treating cartilage defects, but the choice of the optimal cell source is complex. Research is currently focusing on different types of cells to objectively assess their advantages and disadvantages in repairing damaged articular cartilage.
Review
Biotechnology & Applied Microbiology
Yanxi Liu, Karan M. Shah, Jian Luo
Summary: Articular cartilage is avascular and has limited ability to repair itself, leading to degenerative joint diseases. Current clinical treatments often result in mechanically inferior cartilage. Understanding factors affecting cartilage homeostasis and exploring emerging regenerative approaches are crucial for future treatment options.
FRONTIERS IN BIOENGINEERING AND BIOTECHNOLOGY
(2021)
Article
Orthopedics
Hunter Bohlen, Theofilos Karasavvidis, Deborah Wen, Francis K. L. Wong, Dean Wang
Summary: This study compared the 30-day complication rates of isolated cartilage restoration (ICR) and concomitant cartilage restoration and osteotomy (CRO). Multivariate analysis showed no significant differences in rates of pulmonary embolism, venous thromboembolism, and all-cause readmission between the two groups. The CRO group had no events of wound disruption, surgical site infection, or reoperation, while the ICR group had low rates of these complications.