Article
Medicine, General & Internal
Myungsik Nam, Sun Woong Kim
Summary: Corneal epithelial thickness (CET) was found to be smaller in patients with glaucoma receiving topical medications compared to controls. Among various factors influencing CET, the use of beta-blockers, prostaglandin, and frequency of BAK-containing instillations were significant.
JOURNAL OF CLINICAL MEDICINE
(2021)
Article
Cell & Tissue Engineering
Yu Yoshinaga, Takeshi Soma, Shohei Azuma, Kazuichi Maruyama, Yoshiko Hashikawa, Tomohiko Katayama, Yuzuru Sasamoto, Hiroshi Takayanagi, Naoki Hosen, Takashi Shiina, Kazumasa Ogasawara, Ryuhei Hayashi, Kohji Nishida
Summary: This study investigated the impact of human leukocyte antigen (HLA) on corneal epithelial cell sheet (CEPS) transplantation. The results showed that there was no difference in immune response to CEPS between HLA-matched and -unmatched peripheral blood mononuclear cells. Additionally, programmed death ligand 1 was identified to have an immunosuppressive effect on CEPS under inflammatory conditions.
Article
Biotechnology & Applied Microbiology
Ezgi Ulkuseven, David J. McCanna, Lakshman N. Subbaraman, Lyndon W. Jones
Summary: Antimicrobial peptides are a class of polypeptides composed of less than 100 amino acids, demonstrating strong activity against infectious bacteria. Among the peptides studied, polymyxin E and nisin showed potential for treating ocular infections with low toxicity to human corneal epithelial cells.
PROBIOTICS AND ANTIMICROBIAL PROTEINS
(2021)
Article
Cell & Tissue Engineering
David Hui-Kang Ma, Yi-Jen Hsueh, Kevin Sheng-Kai Ma, Yueh-Ju Tsai, Shiang-Fu Huang, Hung-Chi Chen, Chi-Chin Sun, Ming-Tse Kuo, An-Shine Chao, Jui-Yang Lai
Summary: The study presented long-term outcomes of two clinical cases of COMET transplantation, demonstrating the promising potential of collagenase-based suspension culture technique for enhancing the long-term survival of transplanted OMECs.
STEM CELL RESEARCH & THERAPY
(2021)
Article
Engineering, Biomedical
William Marcondes Facchinatto, Joana Galante, Leticia Mesquita, Daniella Souza Silva, Danilo Martins dos Santos, Tiago Bueno Moraes, Sergio Paulo Campana-Filho, Luiz Alberto Colnago, Bruno Sarmento, Jose das Neves
Summary: This study explored the use of novel nanoparticles as carriers for clotrimazole, demonstrating good drug release properties and improved drug permeability in cell monolayer models.
ACTA BIOMATERIALIA
(2021)
Article
Immunology
Mengliang Wu, Laura E. Downie, Lisa J. Hill, Holly R. Chinnery
Summary: The study found that topical decorin can regulate the dynamics of immune cells during the acute phase after corneal injury, promote corneal regeneration, and potentially achieve this by regulating the expression of nerve-related and cytokine genes.
JOURNAL OF NEUROINFLAMMATION
(2022)
Article
Ophthalmology
Joo-Hee Park, Kangmin Lee, Choul Yong Park
Summary: This study investigated the effects of magnetic microparticles on the movement of magnet-controlled human corneal endothelial cells (HCECs). The results showed that magnetic microparticles had a certain impact on cell viability and cell movement, and could increase cell density at the appropriate concentration.
TRANSLATIONAL VISION SCIENCE & TECHNOLOGY
(2023)
Article
Biochemistry & Molecular Biology
Iman Fatima, Akhtar Rasul, Shahid Shah, Malik Saadullah, Nayyer Islam, Ahmed Khames, Ahmad Salawi, Muhammad Masood Ahmed, Yosif Almoshari, Ghulam Abbas, Mohammed A. S. Abourehab, Sajid Mehmood Khan, Zunera Chauhdary, Meshal Alshamrani, Nader Ibrahim Namazi, Demiana M. Naguib
Summary: The occurrence of fungal infections has increased over the past two decades. Traditional treatment methods are ineffective for deep infections. Therefore, there is a need for topical preparations with advanced penetration techniques. This research successfully encapsulated fluconazole in novasomes to improve topical delivery. The FN7 formulation showed high drug release and antifungal activity.
Article
Microbiology
Maizbha U. Ahmed, Mohammad A. K. Azad, Mengyao Li, Darren J. Creek, Meiling Han, Fanfan Zhou, Kim Chan, Qi Tony Zhou, Tony Velkov, Jian Li
Summary: This study found that after 24 hours of treatment, polymyxin significantly reduced taurine and hypotaurine in lung epithelial cells, while significantly depleting reduced glutathione and increasing oxidized glutathione levels. In addition, polymyxins also affect key metabolic pathways such as cellular redox balance, mitochondrial beta-oxidation, and membrane lipid biogenesis.
ANTIMICROBIAL AGENTS AND CHEMOTHERAPY
(2021)
Article
Ophthalmology
Andrew R. Griswold, Hsin-Che Huang, Daniel A. Bachovchin
Summary: The study demonstrates the functional role of NLRP1 inflammasome in human corneal epithelial cells and sheds light on the mechanism through which aberrant inflammasome activation causes corneal damage.
INVESTIGATIVE OPHTHALMOLOGY & VISUAL SCIENCE
(2022)
Article
Multidisciplinary Sciences
Shinya Watanabe, Ryuhei Hayashi, Yuzuru Sasamoto, Motokazu Tsujikawa, Bruce R. Ksander, Markus H. Frank, Andrew J. Quantock, Natasha Y. Frank, Kohji Nishida
Summary: A subpopulation of cells in hiPSC-derived corneal epithelial cell sheets, positive for ABCB5, demonstrate remarkable holoclone-forming capabilities that can be suppressed by ABCB5 blockade. These cells first emerge in 2D eye-like organoids around six weeks of differentiation and display corneal epithelial characteristics, including sustained expression of ABCB5. This study highlights the translational potential of ABCB5-enriched, hiPSC-derived corneal epithelial cell sheets for vision recovery in stem cell-deficient human eyes and represents the first report of holoclone-forming stem cells identified in an hiPSC-derived organoid.
Article
Multidisciplinary Sciences
Fuad Ameen, Saleh AlNAdhari, Ali A. Al-Homaidan
Summary: Multifunctional drugs have great potential in biomedicine. Fungi isolated from the Red Sea and Arabian Gulf coasts show antimicrobial, anticancer, and antioxidant activity, although the activity varies among different marine fungal isolates.
Article
Medicine, General & Internal
Ahmed Esmail, Mohamed Ibrahim, Sara Nage
Summary: This study aimed to evaluate the effect of topical insulin in treating recurrent epithelial corneal erosion. The results showed that topical insulin can promote corneal regeneration, decrease recurrence, and has advantages of excellent tolerance, availability, and cost-effectiveness.
IRISH JOURNAL OF MEDICAL SCIENCE
(2023)
Article
Ophthalmology
Chunxiao Dong, Dulei Zou, Haoyun Duan, Xiangyue Hu, Qingjun Zhou, Weiyun Shi, Zongyi Li
Summary: Stem cell therapy using retinal pigment epithelium (RPE) cells holds promise for treating corneal endothelial dysfunction. The study showed that RPE cells have high structural and functional similarities to corneal endothelial cells (CECs). Injection of primary RPE cells and human embryonic stem cell (hESC)-derived RPE cells effectively restored corneal clarity and thickness in rabbits. This study is the first to demonstrate the feasibility of RPE cells as an equivalent substitute for CECs.
Article
Ophthalmology
Shafi Balal, Nizar Din, Christopher Ashton, Sajjad Ahmad
Summary: The effectiveness of insulin eye drops for treating refractory persistent epithelial defects (PEDs) was evaluated in this study. Results showed that the use of insulin eye drops led to successful resolution of PED in 9 of 11 cases. This study provides evidence for the use of insulin for closure of PEDs in chemical eye injury.
