Review
Biochemistry & Molecular Biology
Christina Bogensperger, Julia Hofmann, Franka Messner, Thomas Resch, Andras Meszaros, Benno Cardini, Annemarie Weissenbacher, Rupert Oberhuber, Jakob Troppmair, Dietmar ofner, Stefan Schneeberger, Theresa Hautz
Summary: Transplantation is limited by the shortage of healthy donor organs, but ex situ machine perfusion has the potential to expand the donor pool. Mesenchymal stem cells are considered as a tool with the potential to improve organs, but clinical benefits have not been conclusively demonstrated.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2021)
Article
Cardiac & Cardiovascular Systems
Mamoru Takahashi, Hei Yu Andrew Cheung, Tatsuaki Watanabe, Ricardo Zamel, Marcelo Cypel, Mingyao Liu, Shaf Keshavjee
Summary: This study aimed to achieve stable 24-hour normothermic ex vivo lung perfusion by examining modifications to the perfusate administration in a large animal model. Results showed that modifying the perfusate and adding total parenteral nutrition significantly extended perfusion time, improved lung function, and reduced inflammatory cytokine production compared to the control group. Further research is needed to develop stable lung support beyond 24 hours.
JOURNAL OF THORACIC AND CARDIOVASCULAR SURGERY
(2021)
Article
Reproductive Biology
Martin Koehne, Christin Unruh, Denny Boettcher, Anna Toenissen, Reiner Ulrich, Harald Sieme
Summary: This study established an ex vivo blood-perfused model for equine uteri and investigated the effects of different cycle stages on the model's applicability. The results showed that the model supported uterine functionality for 6 hours, but viability and histomorphology of the endometrium appeared to be impaired after 4 hours of perfusion. The cycle stage did not affect the applicability of the model.
Article
Multidisciplinary Sciences
Mukhammad Kayumov, In Seok Jeong, Dowan Kim, Yujin Kwak, Francis O. Obiweluozor, Namsik Yoon, Hyo Sin Kim, Hwa Jin Cho
Summary: Heart transplantation is the most effective treatment for end-stage heart failure, but the number of patients awaiting transplantation is still increasing. The normothermic ex situ preservation technique has been shown to be comparable to traditional cold storage, allowing donor hearts to be preserved for up to 12 hours in a physiological condition. We present a rat model of this technique, which is cost-effective and can be completed by a single experimenter.
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2023)
Review
Biotechnology & Applied Microbiology
Kyle D. Kovacs, Thomas A. Ciulla, Szilard Kiss
Summary: Ocular gene therapy is a rapidly evolving field with various strategies and techniques for treating inherited and acquired retinal diseases. Recent advancements in ocular gene therapy have expanded its potential applications and administration techniques, raising the possibility of in-office gene therapy for common retinal disorders in the near future.
EXPERT OPINION ON BIOLOGICAL THERAPY
(2022)
Article
Gastroenterology & Hepatology
Wei Kelly Wu, Rei Ukita, Yatrik J. Patel, Michael Cortelli, Vincent Q. Trinh, Ioannis A. Ziogas, Sean A. Francois, Meredith Mentz, Nancy L. Cardwell, Jennifer R. Talackine, William M. Grogan, John W. Stokes, Youngmin A. A. Lee, Jinho Kim, Sophoclis P. Alexopoulos, Matthew Bacchetta
Summary: Xenogeneic cross-circulation technique has been successfully used to support and rehabilitate declined donor livers, resulting in preservation of organ structure and function, improved viability markers, and potential for further research in organ recovery and rehabilitation.
Article
Biotechnology & Applied Microbiology
Eva Bongaerts, Leonie Aengenheister, Battuja B. Dugershaw, Pius Manser, Maarten B. J. Roeffaers, Marcel Ameloot, Tim S. Nawrot, Hannelore Bove, Tina Buerki-Thurnherr
Summary: The study found that diesel exhaust particles (DEPs) can accumulate in key cell types of the placental tissue and cross the human placenta, although in limited amounts. These findings are crucial for risk assessment and protection of pregnant women, emphasizing the urgent need for further research on the developmental toxicity of ambient particulates mediated by the placenta, both directly and indirectly.
JOURNAL OF NANOBIOTECHNOLOGY
(2021)
Article
Ophthalmology
Xuehui Lu, Guihua Zhang, Lingping Cen, Yali Du, Lifang Liu, Chuang Jin, Haoyu Chen
Summary: The study aims to analyze choroidal vascular density alteration in high myopia with or without choroidal neovascularization using OCTA. Results showed that patients with choroidal neovascularization had higher age, thinner choroidal thickness, and lower choroidal vascular density than those without. Multivariate analysis revealed that decreased choroidal vascular density and older age were independent factors associated with choroidal neovascularization in high myopic eyes.
JOURNAL OF OPHTHALMOLOGY
(2023)
Article
Urology & Nephrology
Jonathan G. Pavlinec, Mark Martin, William Donelan, Elizabeth Kwenda, Paul Dominguez-Gutierrez, Vincent G. Bird, Benjamin K. Canales
Summary: The ex vivo porcine renal perfusion model appears to be a feasible alternative for studying oxalate excretion, as it demonstrates promising results. However, longer validation studies are necessary to establish this technique as a model for kidney stone pathogenesis.
Article
Multidisciplinary Sciences
D. Chan, G. J. Won, A. T. Read, C. R. Ethier, E. Thackaberry, S. R. Crowell, H. Booler, V Bantseev, J. M. Sivak
Summary: This study investigates the movement of particles in the eye during intravitreal drug delivery. Using an organotypic perfusion strategy and experiments in pig and human eyes, it is found that the aggregate particle size plays a crucial role in determining their movement within the eye.
JOURNAL OF THE ROYAL SOCIETY INTERFACE
(2022)
Article
Ophthalmology
Nicholas E. Dudenhoefer, Carl W. Noble, Michael R. Petersen, Robert E. Foster, Christopher D. Riemann, Robert A. Sisk, Robert K. Hutchins, Daniel M. Miller
Summary: This study assessed the long-term efficacy of IVI and IVI/PDT for managing choroidal neovascularization secondary to POHS, finding both treatments effective with IVI/PDT group having a lower average number of annual injections and longer treatment-free interval.
RETINA-THE JOURNAL OF RETINAL AND VITREOUS DISEASES
(2022)
Article
Radiology, Nuclear Medicine & Medical Imaging
Alexis Slama, Benedikt M. Schaarschmidt, Oezlem Okumus, Ken Herrmann, Clemens Aigner, Stephane Collaud, Hubertus Hautzel
Summary: This experiment demonstrates the feasibility and benefits of using ILP combined with PET/CT imaging. The results show that ILP can preserve near physiologic gas exchange and glucose metabolism. The peripheral area of the carcinoid exhibits delayed but high somatostatin receptor agonist uptake compared to the surrounding parenchyma and the intrapulmonary artery, while the central area of the carcinoid shows very low uptake.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2023)
Article
Radiology, Nuclear Medicine & Medical Imaging
Alexis Slama, Benedikt M. Schaarschmidt, Oezlem Okumus, Ken Herrmann, Clemens Aigner, Stephane Collaud, Hubertus Hautzel
Summary: This experiment demonstrates the potential and benefits of using isolated lung perfusion (ILP) combined with positron emission tomography-computed tomography (PET/CT) imaging for real-time visualization of tumors in ventilated and perfused lung tissue. The experiment involved a 49-year-old male patient who underwent lung surgery, and the results showed that ILP combined with PET/CT imaging can provide important insights into tumor physiology and molecular imaging.
QUANTITATIVE IMAGING IN MEDICINE AND SURGERY
(2023)
Article
Ophthalmology
Haoxing D. Jin, John W. Surbeck, Henry R. Marsh, Kai Ding, Ronald M. Kingsley, Kamran M. Riaz, Stanley S. K. Park, Vinay A. Shah
Summary: The study found that dissolvable collagen punctal plugs were not effective in reducing post-injection ocular irritation in patients with mild dry eye symptoms, but showed significant improvement in patients with dry eye symptoms and objective findings, especially in relieving symptoms.
Review
Ophthalmology
Ruchi Goel, Shalin Shah, Gangadhara Sundar, Ritu Arora, Swati Gupta, Tamanna Khullar
Summary: Thyroid eye disease (TED) is characterized by enlargement of extraocular muscles, an increase in retrobulbar fat, orbital fibrosis, and fluctuations in plasma thyroid hormone levels in most patients, often associated with raised autoantibody titers. The occurrence of orbital space conflict compromises the orbital perfusion, resulting in irreversible loss of visual acuity and visual fields. Various imaging techniques can be used to assess the orbital and ocular perfusion in TED, including color Doppler imaging, optical coherence tomography angiography, and optical coherence tomography.
SURVEY OF OPHTHALMOLOGY
(2023)
Article
Veterinary Sciences
N. E. Himebaugh, B. C. Gilger
Summary: This retrospective case series study found that positive leptospiral testing results were associated with younger horses and ocular posterior segment clinical disease, leading to poor prognosis for vision in horses with aqueous humour titres to any leptospiral serotype, especially L. bratislava serovar.
EQUINE VETERINARY EDUCATION
(2022)
Review
Biochemistry & Molecular Biology
Brian C. Gilger, Matthew L. Hirsch
Summary: This paper reviews the role of human leukocyte antigen G (HLA-G) in the eye, explores the potential therapeutic applications of AAV gene transfer and expression of HLA-G in different ocular tissues, and discusses the efficacy of these treatments in animal models of various eye diseases. The findings suggest that AAV-HLA-G could be an effective treatment for immune-mediated, inflammatory, and neovascular diseases of the eye.
INTERNATIONAL JOURNAL OF MOLECULAR SCIENCES
(2022)
Article
Veterinary Sciences
Tara M. Stonex, Ashley E. Zibura, Michael Andres, Brian C. Gilger, Annie Oh
Summary: This study describes the use of 1% polidocanol as the sole treatment for a superficial orbital venous malformation in a horse. The results show that polidocanol treatment was well tolerated and led to clinical improvement in the horse.
VETERINARY OPHTHALMOLOGY
(2022)
Article
Agriculture, Dairy & Animal Science
Jose F. Fernandez-Garayzabal, Stacey LaFrentz, Almudena Casamayor, Eva Abarca, Haitham H. Mohammed, Rosemary S. Cuming, Cova R. Arias, Lucas Dominguez, Ana Vela
Summary: This study identified and characterized a new commensal species, Corynebacterium conjunctivae, recovered from the conjunctival sac of healthy adult horses. The bacterial isolates displayed specific cellular morphology, biochemical properties, and gene sequences. This research provides important insights into the diversity of commensal bacteria in horse eyes.
Article
Veterinary Sciences
Brian C. Gilger
Summary: This article discusses the importance of studying immune-mediated ocular diseases in the development of innovative therapies, such as cell and gene therapy. Recent studies on cell and gene therapy are reviewed to highlight the significance of One Health initiatives in treating ocular diseases. The results suggest that autologous subconjunctival BM-MSC therapy may be a promising alternative treatment for immune-mediated ocular diseases.
AMERICAN JOURNAL OF VETERINARY RESEARCH
(2022)
Article
Veterinary Sciences
Brian C. Gilger
Summary: This article reviews naturally occurring ocular diseases in animals and emphasizes how understanding these diseases contributes to one health initiatives, particularly in the context of common ocular diseases shared by animals and humans.
JAVMA-JOURNAL OF THE AMERICAN VETERINARY MEDICAL ASSOCIATION
(2022)
Article
Multidisciplinary Sciences
Elizabeth Crabtree, Katy Uribe, Sara M. Smith, Darby Roberts, Jacklyn H. Salmon, Jacquelyn J. Bower, Liujiang Song, Prabhakar Bastola, Matthew L. Hirsch, Brian C. Gilger
Summary: This study investigated the therapeutic efficacy of AAV8-Equine-IL10 gene therapy for the treatment of experimental autoimmune uveitis (EAU) in rats. The results showed that AAV8-Equine-IL10 treatment effectively reduced inflammation and cell counts, indicating its potential as a safe and effective treatment for uveitis.
Editorial Material
Veterinary Sciences
Brian C. Gilger
EQUINE VETERINARY EDUCATION
(2023)
Article
Ophthalmology
Nicole E. Himebaugh, James B. Robertson, Keith Weninger, Brian C. Gilger, Bjorn Ekesten, Annie Oh
Summary: The study aimed to assess the transmission of UV radiation through intact enucleated globes of different species using spectrophotometry. The results showed that cats and dogs allowed the most transmission of UV radiation, while pigs and humans allowed the least. A small amount of transmission was detected in the globes of rabbits and horses. These findings will support further vision research for training companion, working, and service animals.
EXPERIMENTAL EYE RESEARCH
(2023)
Article
Veterinary Sciences
Hannah L. Smith, Alix K. Berglund, James B. Robertson, Lauren V. Schnabel, Richard J. McMullen, Brian C. Gilger, Annie Oh
Summary: The study aimed to investigate the effect of gentamicin on CD3+ T-lymphocyte proliferation and cell viability using an in vitro cell culture model. Three adult horses were used for the experiment. Peripheral blood lymphocytes were treated with different concentrations of gentamicin and stimulated to proliferate. Live cell counts and fluorescent intensity data were collected. Statistical analysis showed no significant differences between gentamicin concentrations or formulations in terms of cell viability and proliferation. The results suggest that low-dose intravitreal gentamicin may not suppress the immune response.
VETERINARY OPHTHALMOLOGY
(2023)
Article
Medicine, Research & Experimental
Jacob P. Nilles, Darby Roberts, Jacklyn H. Salmon, Liujiang Song, Carly O'Dea, Lindsay T. Marjoram, Jacquelyn J. Bower, Matthew L. Hirsch, Brian C. Gilger
Summary: This study evaluated the efficacy of adeno-associated virus (AAV) gene therapy encoding human leukocyte antigen G (HLA-G) to inhibit ocular graft versus host disease (OGvHD). The results showed that eyes treated with AAV had significantly reduced clinical signs of OGvHD and lower limbal mononuclear cell count. These findings support the potential of HLA-G-based gene therapy as an effective treatment for OGvHD.
MOLECULAR THERAPY-METHODS & CLINICAL DEVELOPMENT
(2023)
Article
Veterinary Sciences
Elizabeth Esdaile, Kelly E. Knickelbein, Callum G. Donnelly, Michelle Ferneding, Monica J. Motta, Brett D. Story, Felipe Avila, Carrie J. Finno, Brian C. Gilger, Lynne Sandmeyer, Sara Thomasy, Rebecca R. Bellone
Summary: This study identifies the presence of congenital stationary night blindness (CSNB) in multiple horse breeds and identifies the genetic variations associated with this disease. The study also provides recommendations for treatment, emphasizing the importance of genetic testing in horses with the identified genetic variations.
VETERINARY OPHTHALMOLOGY
(2023)
Article
Microbiology
Darby Roberts, Jacklyn Salmon, Marc A. Cubeta, Brian C. Gilger
Summary: This study aims to explore the differences in fungal response to topical antifungal treatment at different stages of fungal keratitis (FK) development. The results show that fungal development stage is correlated with efficacy, and traditional antifungal susceptibility testing methods are not applicable to more advanced developmental stages. A revised method of antifungal susceptibility testing may better predict fungal response in the clinical setting.
Article
Veterinary Sciences
Brian Christopher Gilger
VETERINARY CLINICS OF NORTH AMERICA-EQUINE PRACTICE
(2023)
Article
Cell & Tissue Engineering
Nasif Mahmood, Taylor Cook Suh, Kiran M. Ali, Eelya Sefat, Ummay Mowshome Jahan, Yihan Huang, Brian C. Gilger, Jessica M. Gluck
Summary: Deficiency and dysfunction of corneal cells can lead to blindness in corneal diseases. However, there is a lack of adequate cell sources for regenerative cell therapies and engineered corneal tissue. The discovery of induced pluripotent stem cells (iPSCs) provides an opportunity to generate corneal cells autologously and in unlimited quantities. iPSCs have advantages over other stem cell sources in terms of differentiation potential and ethical concerns. They have been used to model corneal disorders and diseases, perform drug testing, and explore regenerative medicine. Building stem cell banks with HLA-homozygous cell lines can provide cost- and time-efficient allogeneic alternatives to expensive autologous treatments.
STEM CELL REVIEWS AND REPORTS
(2022)
