Journal
JOURNAL OF NEUROTRAUMA
Volume 26, Issue 7, Pages 1029-1042Publisher
MARY ANN LIEBERT, INC
DOI: 10.1089/neu.2008.0785
Keywords
BDNF; cAMP; embryonic stem cells; IL-6; regeneration; spinal cord injury
Funding
- North Carolina Biotechnology Center [2004-MRG-1104]
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The mechanism of embryonic stem (ES) cell therapeutic action remains far from being elucidated. Our recent report has shown that transplantation of ES cells, predifferentiated into neuronal progenitors, prevented appearance of chronic pain behaviors in mice after experimentally induced spinal cord injury. In the current study, we tested the hypothesis that this beneficial effect is mediated by antiapoptotic and regenerative signaling pathways activated in the host tissue by transplanted ES cells. Spinal cord injury was induced by unilateral microinjections of quisqualic acid at spinal levels T12-L2. At 1 week after injury, the pre-differentiated towards neuronal phenotype ES cells were transplanted into the site of injury. Here we show that transplantation of predifferentiated ES cells activate both brain-derived neurotrophic factor (BDNF) and interleukin-6 (IL-6) signaling pathways in the host tissue, leading to activation of cAMP/PKA, phosporylation of cofilin and synapsin I, and promoting regenerative growth and neuronal survival.
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