Journal
JOURNAL OF NEUROSCIENCE RESEARCH
Volume 89, Issue 7, Pages 1079-1090Publisher
WILEY-BLACKWELL
DOI: 10.1002/jnr.22616
Keywords
adhesion; glioma; invasion; ITSN1-s; migration
Categories
Funding
- China 973 Program [2009CB521705]
- 863 Program [2006AA02A249, 2007AA021802]
- National Scientific Foundation of China [30700253, 30800355, 30930038]
- China Program of Changjiang Scholar and Innovative Research Team in University [IRT0743]
Ask authors/readers for more resources
Malignant gliomas have a tendency to invade diffusely into surrounding healthy brain tissues, thereby precluding their successful surgical removal. Intersectin1 (ITSN1) as a molecular linker in the central nervous system is well known as an important regulator of endocytosis and exocytosis. ITSN1 has two isoforms: ITSN1-l and ITSN1-s. In this study, we show that siRNA-mediated down regulation of ITSN1-s inhibited migration and invasion of glioma cells. In addition, we demonstrate the possible mechanisms by which ITSN1-s functions in migration and invasion. Several key proteins, including cofilin, LIMK, PAK, FAK, integrin beta 1, and MMP-9, which are critical for cells migration and invasion, were probably involved in ITSN1-s signaling pathways. These results suggest that ITSN1-s contributes to glioma cells migration and invasion by regulating the formation of cytoskeleton, influencing adhesion and increasing expression of MMP-9. Our results indicate that ITSN1-s is a critical factor in glioms invasion and identify that ITSN1-s is a new potentially antiinvasion target for therapeutic intervention in gliomas. (C) 2011 Wiley-Liss, Inc.
Authors
I am an author on this paper
Click your name to claim this paper and add it to your profile.
Reviews
Recommended
No Data Available