Article
Medicine, General & Internal
Malin Lager, Peter Wilhelmsson, Andreas Matussek, Per-Eric Lindgren, Anna J. Henningsson
Summary: Clinical diagnostics for Lyme neuroborreliosis (LNB) are primarily based on serology with PCR used as a supplemental tool in certain cases. A study evaluated parameters for improving the sensitivity of detecting Borrelia bacteria in CSF through PCR targeting the 16S rRNA gene. Results showed that centrifugation time and speed, use of complementary DNA, and absence of inhibitors had the greatest impact on sensitivity. A protocol for optimized CSF sample handling was proposed based on these findings, but clinical evaluation and further investigations are needed.
Review
Biochemical Research Methods
Rainer Lehmann
Summary: The stability of lipids and other metabolites in human body fluids varies greatly, requiring careful handling during sample collection and processing. Critical pre-analytical steps include patient preparation, collection of body fluids, sample handling, transport, storage, and thawing to ensure valid profiling data for metabolomics and lipidomics investigations. Proper attention to these steps is essential for accurate analysis and assessment of sample quality.
ANALYTICAL AND BIOANALYTICAL CHEMISTRY
(2021)
Article
Biochemistry & Molecular Biology
Johann Zwirner, Sven Anders, Simone Bohnert, Ralph Burkhardt, Ugo Da Broi, Niels Hammer, Dirk Pohlers, Rexson Tse, Enjamin Ondruschka
Summary: The study suggests that using creatine kinase (CK) immunoassays in postmortem cerebrospinal fluid can serve as an adjunct quantitative test aid in diagnosing acute TBI-related fatalities. TBI cases showed a significant increase in CK and CK-MB, with moderate to high diagnostic accuracy.
Article
Biochemistry & Molecular Biology
Hilal A. Rather, Shalini Mishra, Yixin Su, Ashish Kumar, Sangeeta Singh, Biswapriya B. Misra, Jingyun Lee, Cristina M. Furdui, Lindsey R. Hamilton, Robert W. Gould, Susan H. Nader, Michael A. Nader, Gagan Deep
Summary: This study investigates the effects of prenatal cocaine use on postnatal development and long-term neurobiological outcomes. The researchers found that extracellular vesicles (EVs) could potentially serve as biomarkers to assess the adverse effects due to prenatal cocaine exposure.
Article
Multidisciplinary Sciences
Sharifah Zakiah Syed Sulaiman, Wei Miao Tan, Rozanaliza Radzi, Intan Nur Fatiha Shafie, Mokrish Ajat, Rozaihan Mansor, Suhaila Mohamed, Norasfaliza Rahmad, Angela Min Hwei Ng, Seng Fong Lau
Summary: This study compared the proteomic changes in the synovial fluid of surgically and chemically induced osteoarthritis (OA) models. The surgical model showed a wider range of protein changes, while the chemical model had slower OA progression.
Article
Neurosciences
Marion Ortner, Korbinian Lanz, Oliver Goldhardt, Felix Mueller-Sarnowski, Janine Diehl-Schmid, Hans Foerstl, Dennis M. Hedderich, Igor Yakushev, Chad A. Logan, Jan-Philipp Weinberger, Maryline Simon, Timo Grimmer
Summary: Elecsys cerebrospinal fluid (CSF) immunoassays can detect pathological changes in early Alzheimer's disease patients, regardless of concomitant dementia due to small vessel disease. These assays have reliability and sensitivity, and are important for the diagnosis and treatment of patients with early pathological dementia.
JOURNAL OF ALZHEIMERS DISEASE
(2023)
Article
Medical Laboratory Technology
Kaj Blennow, Erik Stomrud, Henrik Zetterberg, Niels Borlinghaus, Veronika Corradini, Ekaterina Manuilova, Laura Muller-Hubner, Frances-Catherine Quevenco, Sandra Rutz, Oskar Hansson
Summary: This study evaluated the analytical performance of second-generation Elecsys CSF Gen II immunoassays and adjusted existing cut-offs to evaluate their potential utility in clinical routine. The findings suggest that the Gen II immunoassays have potential utility in aiding the diagnosis of Alzheimer's disease.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Review
Biochemistry & Molecular Biology
Xin-Yu Ma, Ting-Ting Yang, Lian Liu, Xiao-Chun Peng, Feng Qian, Feng-Ru Tang
Summary: The loss of neurons caused by progressive damage to the nervous system is proposed as the main pathogenesis of neurodegenerative diseases. Ependyma, composed of ciliated ependymal cells, plays a role in the formation of the brain-cerebrospinal fluid barrier (BCB) and the circulation and material exchange of cerebrospinal fluid (CSF) and brain interstitial fluid. Radiation-induced brain injury (RIBI) leads to impairments of the blood-brain barrier (BBB). The vulnerability of ependyma to immune responses may disrupt the integrity of BCB, affecting CSF flow and material exchange, and contributing to the pathogenesis of neurodegenerative diseases.
Article
Pediatrics
Kristin Leifsdottir, Kerstin Jost, Veronica Siljehav, Eric P. Thelin, Philipp Lassaren, Peter Nilsson, Asgeir Haraldsson, Staffan Eksborg, Eric Herlenius
Summary: This study found differences in the protein profiles of cerebrospinal fluid (CSF) between preterm infants and full-term infants. Preterm infants had increased levels of proteins associated with neurodevelopment and neuroinflammatory pathways, which may serve as potential biomarkers for unfavorable neurodevelopmental outcomes in preterm infants. Several proteins were found to be associated with favorable outcomes in preterm infants.
FRONTIERS IN PEDIATRICS
(2022)
Article
Multidisciplinary Sciences
Marlies Gijs, Sinthuja Arumugam, Nienke van de Sande, Carroll A. B. Webers, Swaminathan Sethu, Arkasubhra Ghosh, Rohit Shetty, Jelle Vehof, Rudy M. M. A. Nuijts
Summary: Tear fluid has potential as a non-invasive biomarker source for ocular and systemic conditions, but standardized methods for collecting and processing tear fluid are needed to improve the accuracy of tear protein quantification. This study investigated sample handling factors that may affect tear protein biomarker profiles. Differences in wetting and migration length were observed between Schirmer's strips from different manufacturers and protein-free and protein-rich solutions. The study highlights the importance of standardization in Schirmer's strip manufacturing, tear fluid processing, and analyte concentration normalization.
SCIENTIFIC REPORTS
(2023)
Article
Biochemistry & Molecular Biology
Svitlana Rozanova, Julian Uszkoreit, Karin Schork, Bettina Serschnitzki, Martin Eisenacher, Lars Toenges, Katalin Barkovits-Boeddinghaus, Katrin Marcus
Summary: Proteomic studies using mass spectrometry (MS)-based quantification are important for discovering new biomarkers, but the accuracy of the outcomes can be affected by various analytical conditions. Therefore, it is crucial to assess the quality of the acquired data, including raw data, identification, and quantification levels. This study demonstrates the assessment of four independent datasets from cerebrospinal fluid, highlighting the impact of sample processing-based differences on the quality of quantitative data. Additionally, a free and open source quality control tool called MaCProQC is provided for systematic and rapid data comparison.
Article
Biochemistry & Molecular Biology
Hagen M. Gegner, Thomas Naake, Aurelien Dugourd, Torsten Mueller, Felix Czernilofsky, Georg Kliewer, Evelyn Jaeger, Barbara Helm, Nina Kunze-Rohrbach, Ursula Klingmueller, Carsten Hopf, Carsten Mueller-Tidow, Sascha Dietrich, Julio Saez-Rodriguez, Wolfgang Huber, Rudiger Hell, Gernot Poschet, Jeroen Krijgsveld
Summary: Metabolomic and proteomic analyses of human plasma and serum samples have the potential to advance our understanding of disease biology. However, pre-analytical factors such as temperature and time can impact the integrity of the samples and there is a need for standardized operating procedures to ensure reliable data.
FRONTIERS IN MOLECULAR BIOSCIENCES
(2022)
Article
Medical Laboratory Technology
Mohammed F. F. Alkadhem, Lucie M. F. Wagenmakers-Huizenga, Marjan Wouthuyzen-Bakker, Anneke C. C. Muller Kobold
Summary: This study investigates the stability of synovial calprotectin and its susceptibility to potential interfering factors. It is found that factors such as time, storage temperature, EDTA, freeze-thaw cycles, viscosity, and blood and lipid contamination do not significantly affect the analysis of synovial calprotectin.
CLINICAL CHEMISTRY AND LABORATORY MEDICINE
(2023)
Article
Biochemistry & Molecular Biology
Carolin Kurz, Laura Stoeckl, Isabelle Schrurs, Ivonne Suridjan, Selim Uestuen Guersel, Tobias Bittner, Alexander Jethwa, Robert Perneczky
Summary: An investigation was conducted on the impact of different pre-analytical sample handling procedures on plasma biomarkers for Alzheimer's disease (AD). The effects of storage time, freeze/thaw cycles, anticoagulants, tube transfer, and tube types were evaluated. The results provide recommendations for an optimal sample handling protocol to improve the reproducibility of future studies on plasma biomarkers assays for AD.
JOURNAL OF NEUROCHEMISTRY
(2023)
Article
Mathematical & Computational Biology
Dan Shao, Lan Huang, Yan Wang, Xueteng Cui, Yufei Li, Yao Wang, Qin Ma, Wei Du, Juan Cui
Summary: The study highlights the importance of human body fluid proteome as a source for disease biomarker discovery and the lack of a centralized database for published body fluid proteins. The newly developed Human Body Fluid Proteome (HBFP) database provides a valuable resource for research in clinical proteomics and biomarker discovery.
DATABASE-THE JOURNAL OF BIOLOGICAL DATABASES AND CURATION
(2021)
Article
Medicine, Research & Experimental
Anna Akesson, Veronica Lindstrom, Ulf Nyman, Magnus Jonsson, Magnus Abrahamson, Anders Christensson, Jonas Bjorle, Anders Grubb
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
(2020)
Article
Urology & Nephrology
Jonas Bjork, Ulf Nyman, Marie Courbebaisse, Lionel Couzi, R. Neil Dalton, Laurence Dubourg, Natalie Ebert, Bjorn O. Eriksen, Francois Gaillard, Cyril Garrouste, Anders Grubb, Magnus Hansson, Lola Jacquemont, Ian Jones, Nassim Kamar, Edmund J. Lamb, Christophe Legendre, Karin Littmann, Christophe Mariat, Toralf Melsom, Lionel Rostaing, Andrew D. Rule, Elke Schaeffner, Per-Ola Sundin, Stephen Turner, Anna Akesson, Pierre Delanaye, Hans Pottel
CLINICAL KIDNEY JOURNAL
(2020)
Review
Biotechnology & Applied Microbiology
Sara Diana Lolansen, Nina Rostgaard, Eva Kjer Oernbo, Marianne Juhler, Anja Hviid Simonsen, Nanna MacAulay
Summary: This systematic review evaluated inflammatory markers in CSF from patients with hydrocephalus and found that IL-6, IL-1 beta, LRG, IL-18, VEGF, and IFN-gamma are elevated in these patients, potentially promoting the development and progression of hydrocephalus. These markers may serve as novel disease biomarkers and represent targets for pharmacological management of hydrocephalus.
Article
Biochemical Research Methods
Liana Xhakollari, Amra Jujic, John Molvin, Peter Nilsson, Hannes Holm, Erasmus Bachus, Margret Leosdottir, Anders Grubb, Anders Christensson, Martin Magnusson
Summary: In heart failure patients, the Shrunken Pore Syndrome (SPS) is associated with proteins linked to atherosclerosis and cell proliferation, indicating a potential impact of SPS on heart health.
PROTEOMICS CLINICAL APPLICATIONS
(2021)
Article
Pediatrics
Anders Grubb
Summary: The kidney plays a crucial role in maintaining the steady-state of 5-30 kDa proteins, including signaling molecules, through glomerular filtration. This function has been often underestimated due to the focus on measuring GFR with low-molecular-mass substances <1 kDa. Using cystatin C as a marker for GFR has helped identify the selective impairment of filtration of 5-30 kDa molecules, leading to the discovery of shrunken pore syndrome and its associated morbidity and mortality, mainly affecting cardiovascular health.
Article
Medicine, General & Internal
Lesley A. Inker, Nwamaka D. Eneanya, Josef Coresh, Hocine Tighiouart, Dan Wang, Yingying Sang, Deidra C. Crews, Alessandro Doria, Michelle M. Estrella, Marc Froissart, Morgan E. Grams, Tom Greene, Anders Grubb, Vilmundur Gudnason, Orlando M. Gutierrez, Roberto Kalil, Amy B. Karger, Michael Mauer, Gerjan Navis, Robert G. Nelson, Emilio D. Poggio, Roger Rodby, Peter Rossing, Andrew D. Rule, Elizabeth Selvin, Jesse C. Seegmiller, Michael G. Shlipak, Vicente E. Torres, Wei Yang, Shoshana H. Ballew, Sara J. Couture, Neil R. Powe, Andrew S. Levey
Summary: New equations for estimating GFR without considering race, using cystatin C and creatinine, reduce estimation errors between Black and non-Black participants. Compared to equations using creatinine alone, the new equations without race are more accurate and result in smaller differences between race groups.
NEW ENGLAND JOURNAL OF MEDICINE
(2021)
Article
Cardiac & Cardiovascular Systems
Liana Xhakollari, Anders Grubb, Amra Jujic, Erasmus Bachus, Peter M. Nilsson, Margret Leosdottir, Anders Christensson, Martin Magnusson
Summary: This study found an association between 'Shrunken pore syndrome' (SPS) and increased risk of death, 30 day rehospitalization, as well as impaired health-related quality of life in heart failure patients.
Article
Physiology
Carl M. Oberg, Martin Lindstrom, Anders Grubb, Anders Christensson
Summary: This study found that thickening of the glomerular filter in DKD patients is associated with an increased diffusion length of cystatin C, resulting in a lower eGFR(cystatin)/eGFR(creatinine) ratio. This has important clinical and theoretical implications for further research and understanding of DKD diagnosis and treatment.
PHYSIOLOGICAL REPORTS
(2021)
Article
Pharmacology & Pharmacy
Pierre Delanaye, Jonas Bjork, Marie Courbebaisse, Lionel Couzi, Natalie Ebert, Bjorn O. Eriksen, R. Neil Dalton, Laurence Dubourg, Francois Gaillard, Cyril Garrouste, Anders Grubb, Lola Jacquemont, Magnus Hansson, Nassim Kamar, Edmund J. Lamb, Christophe Legendre, Karin Littmann, Christophe Mariat, Toralf Melsom, Lionel Rostaing, Andrew D. Rule, Elke Schaeffner, Per-Ola Sundin, Ulla B. Berg, Kajsa Asling-Monemi, Luciano Selistre, Anna Akesson, Anders Larsson, Arend Bokenkamp, Hans Pottel, Ulf Nyman
Summary: The Cockcroft-Gault creatinine-based equation is still commonly used for estimating glomerular filtration rate for drug dosage adjustment, but it has been found to perform worse than more recent equations in terms of bias, imprecision and accuracy. Specifically, the CG equation showed poor ability to estimate GFR overall and in analyses stratified for mGFR, age, and BMI, as well as inferior classification of patients in mGFR staging compared to newer equations.
BRITISH JOURNAL OF CLINICAL PHARMACOLOGY
(2022)
Review
Medicine, Research & Experimental
Carl Ottosson Frost, Per Gille-Johnson, Emanuel Blomstrand, Viggo St-Aubin, Felicia Leion, Anders Grubb
Summary: Estimation or measurement of glomerular filtration rate (GFR) is important for patient treatment. Plasma creatinine has been used for GFR estimation since 1926, but it requires race coefficients for improved accuracy. In contrast, cystatin C-based GFR-estimating equations do not require race coefficients and are recommended for reliable estimation. Sex terms may also be controversial, but are not necessary when using cystatin C-based equations for GFR estimation.
SCANDINAVIAN JOURNAL OF CLINICAL & LABORATORY INVESTIGATION
(2022)
Article
Public, Environmental & Occupational Health
Axel Andersson, Erik Hansson, Ulf Ekstrom, Anders Grubb, Magnus Abrahamson, Kristina Jakobsson, Yiyi Xu
Summary: This study explored the relationship between creatinine and cystatin C based estimated glomerular filtration rate (eGFR) in actively working sugarcane cutters. The findings showed that creatinine increases reflected reduced glomerular filtration, while the estimation based on cystatin C differed from creatinine. This discrepancy may be attributed to the limitations of creatinine estimation.
OCCUPATIONAL AND ENVIRONMENTAL MEDICINE
(2022)
Review
Medicine, General & Internal
Linnea Malmgren, Carl Oberg, Emil den Bakker, Felicia Leion, Joanna Siodmiak, Anna Akesson, Veronica Lindstrom, Erik Herou, Alain Dardashti, Liana Xhakollari, Gabriel Grubb, Helena Strevens, Magnus Abrahamson, Johanna Helmersson-Karlqvist, Martin Magnusson, Jonas Bjork, Ulf Nyman, Johan Arnlov, Peter Ridefelt, Torbjorn Akerfeldt, Magnus Hansson, Anna Sjostrom, Johan Martensson, Yoshihisa Itoh, David Grubb, Olav Tenstad, Lars-Olov Hansson, Isleifur Olafsson, Araceli Jarquin Campos, Martin Risch, Lorenz Risch, Anders Larsson, Gunnar Nordin, Hans Pottel, Anders Christensson, Henrik Bjursten, Arend Bokenkamp, Anders Grubb
Summary: Estimation of kidney function is commonly done using endogenous GFR-markers creatinine or cystatin C. The recommendation in 2010 to use both markers in parallel led to the identification of selective glomerular hypofiltration syndromes, which are associated with increased mortality and morbidity. These syndromes, characterized by a selective reduction in the glomerular filtration of certain molecules, such as cystatin C, have proteomic changes that promote the development of atherosclerosis and may require new treatment modalities.
JOURNAL OF INTERNAL MEDICINE
(2023)
Article
Medicine, General & Internal
H. Pottel, J. Bjork, A. D. Rule, N. Ebert, B. O. Eriksen, L. Dubourg, E. Vidal-Petiot, A. Grubb, M. Hansson, E. J. Lamb, K. Littmann, C. Mariat, T. Melsom, E. Schaeffner, P. -O. Sundin, A. Akesson, A. Larsson, E. Cavalier, J. B. Bukabau, E. K. Sumaili, E. Yayo, D. Monnet, M. Flamant, U. Nyman, P. Delanaye
Summary: The study developed a cystatin C-based equation (EKFC eGFRcys) to estimate glomerular filtration rate (GFR). This equation had similar accuracy to a creatinine-based equation (EKFC eGFRcr) and outperformed other commonly used equations. Furthermore, taking the arithmetic mean of EKFC eGFRcr and EKFC eGFRcys further improved the accuracy of GFR estimation.
NEW ENGLAND JOURNAL OF MEDICINE
(2023)
Editorial Material
Urology & Nephrology
Anders O. Grubb, Martin Magnusson, Anders Christensson
AMERICAN JOURNAL OF KIDNEY DISEASES
(2023)
Article
Urology & Nephrology
Lesley A. Inker, Hocine Tighiouart, Ogechi M. Adingwupu, Michael G. Shlipak, Alessandro Doria, Michelle M. Estrella, Marc Froissart, Vilmundur Gudnason, Anders Grubb, Roberto Kalil, Michael Mauer, Peter Rossing, Jesse Seegmiller, Josef Coresh, Andrew S. Levey
Summary: New CKD-EPI and EKFC eGFR equations have sufficient accuracy for clinical practice. Differences in performance across subgroups reflect diversity in source populations and use of variables. Using eGFR equations with both cystatin C and creatinine can improve accuracy and uniformity of GFR estimation.
JOURNAL OF THE AMERICAN SOCIETY OF NEPHROLOGY
(2023)
Article
Biochemical Research Methods
Aline Silva da Cruz, Maria Margarida Drehmer, Wagner Baetas-da-Cruz, Joao Carlos Machado
Summary: This study quantified microcirculation cerebral blood flow in a rat model of ischemic stroke using ultrasound biomicroscopy and ultrasound contrast agents. The results showed high sensitivity and specificity of this method, making it a valuable tool for preclinical studies.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Christina Dalla, Ivana Jaric, Pavlina Pavlidi, Georgia E. Hodes, Nikolaos Kokras, Anton Bespalov, Martien J. Kas, Thomas Steckler, Mohamed Kabbaj, Hanno Wuerbel, Jordan Marrocco, Jessica Tollkuhn, Rebecca Shansky, Debra Bangasser, Jill B. Becker, Margaret McCarthy, Chantelle Ferland-Beckham
Summary: Many funding agencies have emphasized the importance of considering sex as a biological variable in experimental design to improve the reproducibility and translational relevance of preclinical research. Omitting the female sex from experimental designs in neuroscience and pharmacology can result in biased or limited understanding of disease mechanisms. This article provides methodological considerations for incorporating sex as a biological variable in in vitro and in vivo experiments, including the influence of age and hormone levels, and proposes strategies to enhance methodological rigor and translational relevance in preclinical research.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Wenyu Gu, Dongxu Li, Jia-Hong Gao
Summary: We developed a precise and rapid method for positioning and labelling triaxial OPMs on a wearable magnetoencephalography (MEG) system, improving the efficiency of OPM positioning and labelling.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Kai Lin, Linhang Zhang, Jing Cai, Jiaqi Sun, Wenjie Cui, Guangda Liu
Summary: The article introduces an EEG feature map processing model for emotion recognition, which achieves significantly improved accuracy by fusing EEG information at different spatial scales and introducing a channel attention mechanism.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
John E. Parker, Asier Aristieta, Aryn H. Gittis, Jonathan E. Rubin
Summary: This work presents a toolbox that implements a methodology for automated classification of neural responses based on spike train recordings. The toolbox provides a user-friendly and efficient approach to detect various types of neuronal responses that may not be identified by traditional methods.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Yun Liang, Ke Bo, Sreenivasan Meyyappan, Mingzhou Ding
Summary: This study compared the performance of SVM and CNN on the same datasets and found that CNN achieved consistently higher classification accuracies. The classification accuracies of SVM and CNN were generally not correlated, and the heatmaps derived from them did not overlap significantly.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Antonino Visalli, Maria Montefinese, Giada Viviani, Livio Finos, Antonino Vallesi, Ettore Ambrosini
Summary: This study introduces an analytical strategy that allows the use of mixed-effects models (LMM) in mass univariate analyses of EEG data. The proposed method overcomes the computational costs and shows excellent performance properties, making it increasingly important in the field of neuroscience.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Xavier Cano-Ferrer, Alexandra Tran -Van -Minh, Ede Rancz
Summary: This study developed a novel rotation platform for studying neural processes and spatial navigation. The platform is modular, affordable, and easy to build, and can be driven by the experimenter or animal movement. The research demonstrated the utility of the platform, which combines the benefits of head fixation and intact vestibular activity.
JOURNAL OF NEUROSCIENCE METHODS
(2024)