Article
Virology
Liling Zhou, Chuncong Mo, Yujie Yang, Zhichao Zhou, Aiping You, Ye Fan, Wenkuan Liu, Xiao Li, Rong Zhou, Xingui Tian
Summary: The ongoing COVID-19 pandemic has had a significant impact on the world, emphasizing the importance of accurate virus detection. In this study, researchers generated 90 monoclonal antibodies that exclusively reacted with the nucleocapsid protein of SARS-CoV-2. They identified major epitopes using enzyme-linked immunoassay and found that some antibodies showed no cross-reaction with other coronaviruses. These antibodies were used for antigen detection, and novel B-cell epitopes of the viral nucleocapsid protein were identified.
JOURNAL OF MEDICAL VIROLOGY
(2023)
Article
Chemistry, Applied
Yifang Cui, Huiwen Wang, Fangfang Guo, Xiaoya Cao, Xue Wang, Ximin Zeng, Guolin Cui, Jun Lin, Fuzhou Xu
Summary: This study developed a monoclonal antibody-based ELISA method for quantifying Enterobacteriaceae levels in the intestine, which can be used to assess gut inflammation. The ELISA showed high accuracy, repeatability, and reproducibility, and was able to detect Enterobacteriaceae levels produced in different bacterial cultures.
Article
Food Science & Technology
Wanyao Song, Mengyu Luo, Huaming Li, Jiaxu Xiao, Xiuping He, Jixiang Liang, Dapeng Peng
Summary: In this study, an ic-ELISA method was developed using a new generation metabolite (DMEQ)-based monoclonal antibody (mAb) for the rapid detection of Qx residues in food. The method exhibited high sensitivity and a wide linear range, and showed good cross-reactivity with multiple DQx. Furthermore, the method demonstrated good correlation with LC-MS/MS results in animal-derived foods, indicating its potential for rapid screening of Qx residues.
Article
Food Science & Technology
Yaozhou Zhu, Yun-Hwa Peggy Hsieh
Summary: This study successfully detected a wide spectrum of commercially important fish species using three specific monoclonal antibodies in the indirect enzyme-linked immunosorbent assay, exhibiting high sensitivity, specificity, and reproducibility.
Article
Plant Sciences
Kaewta Rattanapisit, Tharita Kitisripanya, Atthaphon Konyanee, Worapol Sae-Foo, Apisit Burapapiruin, Waraporn Putalun, Seiichi Sakamoto, Waranyoo Phoolcharoen, Gorawit Yusakul
Summary: The plant expression platform is a new source for producing IgG targeting small low-molecular-weight molecules. The study demonstrated that plant-produced functional IgG maintained the binding activity and specificity of IgG derived from mammalian cells, showing potential as an efficient and cost-effective platform for antibody production in immunoassays targeting low-molecular-weight compounds.
PLANT CELL REPORTS
(2021)
Article
Engineering, Chemical
Sobhana A. Sripada, Driss Elhanafi, Leonard B. Collins, Taufika I. Williams, Marina Y. Linova, John M. Woodley, Cristiana Boi, Stefano Menegatti
Summary: This study presents the development and validation of a novel adsorbent, PichiaGuard, which is functionalized with peptide ligands and targets the whole spectrum of K. phaffii HCPs for protein purification. The PichiaGuard adsorbent has high HCP binding capacity and successfully purified monoclonal antibodies and fragments, showing its potential for modern biopharmaceutical processing.
SEPARATION AND PURIFICATION TECHNOLOGY
(2023)
Article
Cell Biology
Carina Z. Segato-Vendrameto, Camila Zanluca, Amanda Z. Zucoloto, Tiago H. Zaninelli, Mariana M. Bertozzi, Telma Saraiva-Santos, Camila R. Ferraz, Larissa Staurengo-Ferrari, Stephanie Badaro-Garcia, Marilia F. Manchope, Amanda M. Dionisio, Felipe A. Pinho-Ribeiro, Sergio M. Borghi, Ana Luiza Pamplona Mosimann, Rubia Casagrande, Juliano Bordignon, Victor Fattori, Claudia N. Duarte dos Santos, Waldiceu A. Verri
Summary: This article investigates therapeutic strategies against Chikungunya virus, finding that inhibiting viral proteins and using recombinant antibodies can alleviate virus-induced pain symptoms.
Article
Food Science & Technology
Shibei Shao, Wenhua Shang, Yuchen Bai, Leina Dou, Suxia Zhang, Jianzhong Shen, Zhanhui Wang, Kai Wen
Summary: In this study, a highly specific and high affinity monoclonal antibody 8A9 was prepared for the detection of diacetoxyscirpenol (DAS), a type A trichothecene, in rice samples. An indirect competitive enzyme-linked immunosorbent assay (ic-ELISA) and a lateral flow immunoassay (LFIA) based on the monoclonal antibody were developed, with detection limits as low as 0.65 μg/kg and 100 μg/kg, respectively. Molecular docking analysis revealed the molecular recognition mechanism of the antibody, demonstrating the highly specific recognition of DAS through hydrogen bonds and a pi-sigma bond.
Article
Microbiology
Xueqing Ma, Jiaxin Huang, Kun Li, Kailu Ding, Yuanfang Fu, Jing Zhang, Zhixun Zhao, Pinghua Li, Xingwen Bai, Dong Li, Xia Liu, Qiaoying Zeng, Zaixin Liu, Pu Sun, Zengjun Lu
Summary: In this study, two porcine-derived monoclonal antibodies against SVA were generated, and a competitive ELISA for the detection of neutralizing antibodies against SVA was successfully developed using these two porcine monoclonal antibodies. The NAC-ELISA was SVA specific with high sensitivity and specificity, and can be used for serodiagnosis and surveillance of SVA, thus contributing to the prevention and control of SVA.
MICROBIOLOGY SPECTRUM
(2023)
Article
Chemistry, Analytical
Rui Feng, Mian Wang, Jingqi Qian, Qingqing He, Man Zhang, Jiaqi Zhang, Hongwei Zhao, Baomin Wang
Summary: A new method for detecting paraquat, a highly toxic herbicide, in adulterated herbicides was developed using icELISA and LFIA. The LFIA showed high accuracy and reliability for rapid screening of paraquat in adulterated herbicide products.
MICROCHEMICAL JOURNAL
(2022)
Article
Veterinary Sciences
Yunlei Cao, Dongmei Han, Yujiao Zhang, Kuan Zhang, Nannan Du, Wu Tong, Guoxin Li, Hao Zheng, Changlong Liu, Fei Gao, Guangzhi Tong
Summary: African swine fever (ASF) is a deadly viral disease affecting pigs with no commercial vaccines available. A monoclonal antibody (mAb) against the p54 protein of ASFV was successfully obtained and shown to have potential for detecting ASF vaccine strains. The synthetic epitope peptide used in the ELISA showed high sensitivity and specificity, indicating promise for ASF epidemic monitoring and control.
RESEARCH IN VETERINARY SCIENCE
(2021)
Article
Chemistry, Applied
Sol-A Kim, Sazzad Hossen Toushik, Jeong-Eun Lee, Won-Bo Shim
Summary: To protect consumers with buckwheat allergies, a high-sensitivity detection method was developed using thermally stable-soluble proteins (TSSPs) in buckwheat to produce monoclonal antibodies (MAbs). The MAbs cocktail solution was used to enhance the sensitivity of an indirect enzyme-linked immunosorbent assay (iELISA), allowing for the successful detection of buckwheat adulteration in processed foods. This study suggests that TSSPs can serve as suitable immunogens and MAbs as bioreceptors for developing immunoassays and biosensors in food facilities.
Article
Engineering, Environmental
Sumei Ling, Qiang Zhao, Muhammad Naeem Iqbal, Mingke Dong, Xiulan Li, Ming Lin, Rongzhi Wang, Feiya Lei, Caizhen He, Shihua Wang
Summary: The study successfully developed an immunoassay method based on mAb for the detection of cadmium ions, which has a wide linear range and high sensitivity. Therefore, it has great potential in monitoring cadmium ions in environmental samples.
JOURNAL OF HAZARDOUS MATERIALS
(2021)
Article
Biotechnology & Applied Microbiology
Madhusudan Hosamani, Shreya Gopinath, B. P. Sreenivasa, Subhasmita Behera, Suresh H. Basagoudanavar, Ashok Boora, Durlav P. Bora, Pankaj Deka, Veerakyathappa Bhanuprakash, Raj Kumar Singh, Aniket Sanyal, Klaas Weerdmeester, Aldo Dekker
Summary: This study developed a new NSP antibody blocking ELISA test and validated it on large panels of sera from different susceptible species. The test showed high diagnostic sensitivity and specificity, making it applicable for a wide range of animals.
APPLIED MICROBIOLOGY AND BIOTECHNOLOGY
(2022)
Article
Chemistry, Analytical
Qingqing He, Mian Wang, Yajie Zhao, Guiyu Tan, Man Zhang, Rui Feng, Yujie Chen, Baomin Wang, Qing X. Li
Summary: To improve the selection efficiency of nanobodies against atrazine in a phage display library, anti-carrier protein phages were depleted using immunomagnetic beads conjugated with bovine serum albumin. This depletion strategy tripled the number of atrazine-positive phage clones and resulted in the selection of a sensitive phage clone with greater thermostability and organic solvent tolerance compared to a monoclonal antibody. The Nb3-based immunoassay showed similar specificity but higher IC50 and method detection limit compared to the mAb 5D9-based immunoassay.
ANALYTICA CHIMICA ACTA
(2023)
Article
Biochemical Research Methods
Xiaoning Li, Steven B. Geng, Mark L. Chiu, Dorina Saro, Peter M. Tessier
BIOCONJUGATE CHEMISTRY
(2015)
Article
Biochemistry & Molecular Biology
Stephen W. Jarantow, Barbara S. Bushey, Jose R. Pardinas, Ken Boakye, Eilyn R. Lacy, Renouard Sanders, Manuel A. Sepulveda, Sheri L. Moores, Mark L. Chiu
JOURNAL OF BIOLOGICAL CHEMISTRY
(2015)
Article
Oncology
Sheri L. Moores, Mark L. Chiu, Barbara S. Bushey, Kristen Chevalier, Leopoldo Luistro, Keri Dorn, Randall J. Brezski, Peter Haytko, Thomas Kelly, Sheng-Jiun Wu, Pauline L. Martin, Joost Neijssen, Paul W. H. I. Parren, Janine Schuurman, Ricardo M. Attar, Sylvie Laquerre, Matthew V. Lorenzi, G. Mark Anderson
Article
Biochemistry & Molecular Biology
Mark L. Chiu, Gary L. Gilliland
CURRENT OPINION IN STRUCTURAL BIOLOGY
(2016)
Article
Endocrinology & Metabolism
Anne M. Cieniewicz, Thomas Kirchner, Simon A. Hinke, Rupesh Nanjunda, Katharine D'Aquino, Ken Boayke, Philip R. Cooper, Robert Perkinson, Mark L. Chiu, Stephen Jarantow, Dana L. Johnson, Jean M. Whaley, Eilyn R. Lacy, Russell B. Lingham, Yin Liang, Anthony J. Kihm
Article
Biochemistry & Molecular Biology
Di Zhang, Monica V. Goldberg, Mark L. Chiu
JOURNAL OF BIOLOGICAL CHEMISTRY
(2016)
Article
Medicine, Research & Experimental
Songmao Zheng, Sheri Moores, Stephen Jarantow, Jose Pardinas, Mark Chiu, Honghui Zhou, Weirong Wang
Article
Medicine, Research & Experimental
Katharine D. Grugan, Keri Dorn, Stephen W. Jarantow, Barbara S. Bushey, Jose R. Pardinas, Sylvie Laquerre, Sheri L. Moores, Mark L. Chiu
Article
Biochemical Research Methods
Suparna Paul, Judy Connor, Tom Nesspor, Peter Haytko, Ken Boakye, Mark L. Chiu, Haiyan Jiang
PROTEIN EXPRESSION AND PURIFICATION
(2016)
Article
Medicine, Research & Experimental
Di Zhang, Anthony A. Armstrong, Susan H. Tam, Stephen G. McCarthy, Jinquan Luo, Gary L. Gilliland, Mark L. Chiu
Article
Multidisciplinary Sciences
Aran F. Labrijn, Joyce I. Meesters, Matthew Bunce, Anthony A. Armstrong, Sandeep Somani, Tom C. Nesspor, Mark L. Chiu, Isil Altintas, Sandra Verploegen, Janine Schuurman, Paul W. H. I. Parren
SCIENTIFIC REPORTS
(2017)
Article
Medicine, Research & Experimental
Di Zhang, Brian Whitaker, Mehabaw G. Derebe, Mark L. Chiu
Article
Endocrinology & Metabolism
Simon A. Hinke, Anne M. Cieniewicz, Thomas Kirchner, Katharine D'Aquino, Rupesh Nanjunda, Jason Aligo, Robert Perkinson, Philip Cooper, Ken Boayke, Mark L. Chiu, Steve Jarantow, Eilyn R. Lacy, Yin Liang, Dana L. Johnson, Jean M. Whaley, Russell B. Lingham, Anthony J. Kihm
MOLECULAR METABOLISM
(2018)
Review
Immunology
Mark L. Chiu, Dennis R. Goulet, Alexey Teplyakov, Gary L. Gilliland
Article
Biochemistry & Molecular Biology
Dennis R. Goulet, Adam Zwolak, Mark L. Chiu, Abhinav Nath, William M. Atkins
Article
Biochemical Research Methods
Aline Silva da Cruz, Maria Margarida Drehmer, Wagner Baetas-da-Cruz, Joao Carlos Machado
Summary: This study quantified microcirculation cerebral blood flow in a rat model of ischemic stroke using ultrasound biomicroscopy and ultrasound contrast agents. The results showed high sensitivity and specificity of this method, making it a valuable tool for preclinical studies.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Christina Dalla, Ivana Jaric, Pavlina Pavlidi, Georgia E. Hodes, Nikolaos Kokras, Anton Bespalov, Martien J. Kas, Thomas Steckler, Mohamed Kabbaj, Hanno Wuerbel, Jordan Marrocco, Jessica Tollkuhn, Rebecca Shansky, Debra Bangasser, Jill B. Becker, Margaret McCarthy, Chantelle Ferland-Beckham
Summary: Many funding agencies have emphasized the importance of considering sex as a biological variable in experimental design to improve the reproducibility and translational relevance of preclinical research. Omitting the female sex from experimental designs in neuroscience and pharmacology can result in biased or limited understanding of disease mechanisms. This article provides methodological considerations for incorporating sex as a biological variable in in vitro and in vivo experiments, including the influence of age and hormone levels, and proposes strategies to enhance methodological rigor and translational relevance in preclinical research.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Wenyu Gu, Dongxu Li, Jia-Hong Gao
Summary: We developed a precise and rapid method for positioning and labelling triaxial OPMs on a wearable magnetoencephalography (MEG) system, improving the efficiency of OPM positioning and labelling.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Kai Lin, Linhang Zhang, Jing Cai, Jiaqi Sun, Wenjie Cui, Guangda Liu
Summary: The article introduces an EEG feature map processing model for emotion recognition, which achieves significantly improved accuracy by fusing EEG information at different spatial scales and introducing a channel attention mechanism.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
John E. Parker, Asier Aristieta, Aryn H. Gittis, Jonathan E. Rubin
Summary: This work presents a toolbox that implements a methodology for automated classification of neural responses based on spike train recordings. The toolbox provides a user-friendly and efficient approach to detect various types of neuronal responses that may not be identified by traditional methods.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Yun Liang, Ke Bo, Sreenivasan Meyyappan, Mingzhou Ding
Summary: This study compared the performance of SVM and CNN on the same datasets and found that CNN achieved consistently higher classification accuracies. The classification accuracies of SVM and CNN were generally not correlated, and the heatmaps derived from them did not overlap significantly.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Antonino Visalli, Maria Montefinese, Giada Viviani, Livio Finos, Antonino Vallesi, Ettore Ambrosini
Summary: This study introduces an analytical strategy that allows the use of mixed-effects models (LMM) in mass univariate analyses of EEG data. The proposed method overcomes the computational costs and shows excellent performance properties, making it increasingly important in the field of neuroscience.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
Article
Biochemical Research Methods
Xavier Cano-Ferrer, Alexandra Tran -Van -Minh, Ede Rancz
Summary: This study developed a novel rotation platform for studying neural processes and spatial navigation. The platform is modular, affordable, and easy to build, and can be driven by the experimenter or animal movement. The research demonstrated the utility of the platform, which combines the benefits of head fixation and intact vestibular activity.
JOURNAL OF NEUROSCIENCE METHODS
(2024)
