Article
Biochemical Research Methods
Khaled Youssef, Daphne Archonta, Terrance J. Kubiseski, Anurag Tandon, Pouya Rezai
Summary: This paper reveals the novel effect of electric field on adult C. elegans egg-laying behavior, showing that the electric egg-laying rate is significantly influenced by EF strength, direction, and exposure duration, as well as worm aging. The involvement and sensitivity of specific neurons and muscles to the EF are demonstrated, and the assay can be used for cellular screening and mapping of the neural basis of electrosensation.
Article
Chemistry, Physical
Siyi Zhuang, Mubin He, Jiaqi Feng, Shiyi Peng, Haochen Jiang, Yunhao Li, Ning Hua, Yujie Zheng, Qizhen Ye, Miaojin Hu, Ying Nie, Peilin Yu, Xiaomin Yue, Jun Qian, Wei Yang
Summary: A noninvasive NIR photothermal technique is developed to modulate cellular excitability and animal behaviors in vivo via the TRPV1 channel with the FDA-approved photothermal agent ICG. The study demonstrates the application of this technique in different types of muscle cells, providing potential implications in mammals and clinical therapy.
Article
Environmental Sciences
Khaled Youssef, Daphne Archonta, Terrance J. Kubiseski, Anurag Tandon, Pouya Rezai
Summary: This study introduced a novel microfluidic electric egg-laying assay for phenotypical assessment of multiple worms in parallel. The effects of glucose and polystyrene microplastics on worm behavior and neurodegeneration were studied, revealing significant impacts on egg-laying and growth. It also highlighted intra-population variability in microplastics uptake and its correlation with physiological and behavioral phenotypes at a single-worm level.
SCIENCE OF THE TOTAL ENVIRONMENT
(2021)
Article
Biology
Sean Harrington, Jessica J. Knox, Andrew R. Burns, Ken-Loon Choo, Aaron Au, Megan Kitner, Cecile Haeberli, Jacob Pyche, Cassandra D'Amata, Yong-Hyun Kim, Jonathan R. Volpatti, Maximillano Guiliani, Jamie Snider, Victoria Wong, Bruna M. Palmeira, Elizabeth M. Redman, Aditya S. Vaidya, John S. Gilleard, Igor Stagljar, Sean R. Cutler, Daniel Kulke, James J. Dowling, Christopher M. Yip, Jennifer Keiser, Inga Zasada, Mark Lautens, Peter J. Roy
Summary: Nematode parasites have a significant impact on human health, livestock, and crops. Researchers have discovered a compound called nementin that selectively kills and immobilizes these parasites. Nementin enhances cholinergic signaling and has the potential to reduce the environmental impact of toxic acetylcholinesterase inhibitors used to control nematode infections.
COMMUNICATIONS BIOLOGY
(2022)
Article
Biochemistry & Molecular Biology
Xue Mei, Katherine A. Maniates, Amaya Looper, Amber R. Krauchunas, Marina Druzhinina, Sunny Dharia, Julie Ni, Gunasekaran Singaravelu, Sam Guoping Gu, Diane C. Shakes, Barth D. Grant, Andrew W. Singson
Summary: Fertilization is a crucial process in sexual reproduction that involves the fusion of gametes and the initiation of a new life cycle. However, the molecular mechanisms underlying sperm-egg interaction are not well understood. In a genetic study using Caenorhabditis elegans, researchers identified the spe-51 gene, which codes for a secreted protein involved in the interactions between sperm and egg. This study provides the first experimental evidence of a secreted protein required for fertilization and highlights the importance of sperm-secreted proteins in influencing sperm function.
Article
Multidisciplinary Sciences
Xue Yan Ho, Sean Coakley, Rumelo Amor, Victor Anggono, Massimo A. Hilliard
Summary: This study uncovers the key role of metalloprotease ADM-4 in axonal fusion, providing a molecular target for axonal repair.
Article
Genetics & Heredity
Bhavya Ravi, Jian Zhao, Sana Chaudhry, Rossana Signorelli, Mattingly Bartole, Richard J. Kopchock, Christian Guijarro, Joshua M. Kaplan, Lijun Kang, Kevin M. Collins
Summary: The study demonstrates that G alpha(o) signaling within the HSN inhibits egg-laying circuit activity, preventing entry into the active state. Loss of inhibitory G alpha o signaling causes precocious entry into the egg-laying active state. Feedback mechanisms involving vulval opening and egg release activate neuroendocrine cells.
Article
Biochemistry & Molecular Biology
Amal Widaad, Ihsan Nazurah Zulkipli, Mark I. R. Petalcorin
Summary: Mimosine in the Leucaena leucocephala (LL) extract has a significant anthelmintic effect on Caenorhabditis elegans (C. elegans), causing worm mortality and reducing head thrashing, egg-laying, and pharyngeal pumping activities.
Article
Multidisciplinary Sciences
Mark G. Sterken, Marijke H. van Wijk, Elizabeth C. Quamme, Joost A. G. Riksen, Lucinda Carnell, Laura D. Mathies, Andrew G. Davies, Jan E. Kammenga, Jill C. Bettinger
Summary: The study examined the transcriptional responses of Caenorhabditis elegans to ethanol exposure across different timepoints, revealing that short exposures induced expression of metabolic enzymes while longer exposures had more profound effects on the transcriptome. Genes involved in cilia function were regulated by longer ethanol exposure, which may play a role in the deleterious effects of chronic ethanol consumption in humans. Additionally, food deprivation-induced gene expression changes were somewhat alleviated by ethanol, supporting previous observations that worms can use ethanol as a calorie source.
SCIENTIFIC REPORTS
(2021)
Article
Genetics & Heredity
Pravat Dhakal, Sana Chaudhry, Rossana Signorelli, Kevin M. Collins
Summary: The study demonstrates the essential roles of phospholipase-C beta and Trio Rho GTPase exchange factor in the egg-laying behavior of Caenorhabditis elegans, coordinating cellular responses to neurotransmitter signals by regulating neurotransmitter release and postsynaptic muscle excitability.
Article
Genetics & Heredity
Soren Emerson, Megan Hay, Mark Smith, Ricky Granger, David Blauch, Nicole Snyder, Rachid El Bejjani
Summary: This study explored the cholinergic effects of cocaine in the egg laying circuit of Caenorhabditis elegans, revealing a novel cocaine-induced egg laying behavior. Results showed that cocaine-stimulated egg laying is dependent on acetylcholine synthesis and release, functional nicotinic acetylcholine receptors, and acetylcholinesterases in C. elegans.
G3-GENES GENOMES GENETICS
(2021)
Article
Genetics & Heredity
Chuanman Zhou, Qian Zhou, Xiaohui He, Yunxia He, Xiaoqin Wang, Xiaowei Zhu, Yujia Zhang, Long Ma
Summary: This study investigates the effects of K2P and NALCN on behavior using the nematode Caenorhabditis elegans as a model. It finds that the K2P gene twk-40 can regulate motor behavior in C. elegans, and identifies five other K2P genes that may have similar effects. Additionally, it suggests that some K2P and NALCN may oppositely affect behavior in C. elegans.
Article
Biotechnology & Applied Microbiology
Ameni Loulou, Noura Chihani Hammas, Sadreddine Kallel
Summary: The amenability to axenic culture in vitro of several nematode species (Oscheius tipulae, Caenorhabditis elegans, Acrobeloides tricornis, Acrobeloides bodenheimeri and Teratorhabditis synpapillata) was tested on different solid and liquid media. The results showed that the nematodes were unable to grow in MacConkey, soybean casein, and nutrient broth media, except for C. elegans which could multiply in soybean casein medium. However, all nematodes could be successfully mass cultured using egg yolk medium. Furthermore, egg yolk liquid medium supported the reproduction of O. tipulae, A. bodenheimeri, and A. tricornis, but not T. synpapillata.
BIOCONTROL SCIENCE AND TECHNOLOGY
(2023)
Article
Zoology
Hannah J. Wileman, Roland N. Perry, Keith G. Davies
Summary: Plant-parasitic nematodes deposit all necessary nutrients, including proteins like vitellin, within their eggs to support the development and survival of their offspring. The vitellin protein is formed from a precursor protein called vitellogenin and has various roles in lipid transport, amino acid provision, and post-embryonic development. While vitellogenin genes have been well studied in Caenorhabditis elegans, not much is known about vitellogenin in plant-parasitic nematodes.
Article
Biology
Kevin M. Collins, Addys Bode, Robert W. Fernandez, Jessica E. Tanis, Jacob C. Brewer, Matthew S. Creamer, Michael R. Koelle
Article
Genetics & Heredity
Navonil Banerjee, Raja Bhattacharya, Michael Gorczyca, Kevin M. Collins, Michael M. Francis
Article
Neurosciences
Bhavya Ravi, Jessica Garcia, Kevin M. Collins
JOURNAL OF NEUROSCIENCE
(2018)
Article
Chemistry, Multidisciplinary
Sicheng Tang, Yang Zhang, Pravat Dhakal, Laura Ravelo, Cheyenne L. Anderson, Kevin M. Collins, Francisco M. Raymo
JOURNAL OF THE AMERICAN CHEMICAL SOCIETY
(2018)
Article
Multidisciplinary Sciences
Bhavya Ravi, Layla M. Nassar, Richard J. Kopchock, Pravat Dhakal, Michael Scheetz, Kevin M. Collins
JOVE-JOURNAL OF VISUALIZED EXPERIMENTS
(2018)
Article
Biology
Pengpeng Li, Kevin M. Collins, Michael R. Koelle, Kang Shen
Article
Genetics & Heredity
Jacob C. Brewer, Andrew C. Olson, Kevin M. Collins, Michael R. Koelle
Article
Neurosciences
Richard J. I. I. I. I. I. I. Kopchock, Bhavya Ravi, Addys Bode, Kevin M. Collins
Summary: The VC motor neurons play a crucial role in the contraction of vulval muscles and egg laying in response to serotonin. The coordination between presynaptic VC neurons and postsynaptic vulval muscles facilitates efficient execution of egg-laying behavior.
JOURNAL OF NEUROSCIENCE
(2021)
Article
Ecology
Bo Zhang, King-Yeung Lam, Wei-Ming Ni, Rossana Signorelli, Kevin M. Collins, Zhiyuan Fu, Lu Zhai, Yuan Lou, Donald L. DeAngelis, Alan Hastings, Jonathan Chase
Summary: This study experimentally confirmed the theory that species with slower diffusion prevail in heterogeneous environments, and found that moderate levels of directed movement allow species to coexist. These results broaden the theory of species coexistence in heterogeneous space and provide empirical confirmation of mathematical predictions.
Article
Genetics & Heredity
Pravat Dhakal, Sana Chaudhry, Rossana Signorelli, Kevin M. Collins
Summary: The study demonstrates the essential roles of phospholipase-C beta and Trio Rho GTPase exchange factor in the egg-laying behavior of Caenorhabditis elegans, coordinating cellular responses to neurotransmitter signals by regulating neurotransmitter release and postsynaptic muscle excitability.
Article
Chemistry, Multidisciplinary
Catherine J. J. Munro, Michelle A. A. Nguyen, Christian Falgons, Sana Chaudhry, Mary O. O. Olagunju, Addys Bode, Carla Bobe, Manuel E. E. Portela, Marc R. R. Knecht, Kevin M. M. Collins
ENVIRONMENTAL SCIENCE-NANO
(2020)
Proceedings Paper
Engineering, Biomedical
Ek Raj Thapaliya, Yang Zhang, Pravat Dhakal, Adrienne S. Brown, James N. Wilson, Kevin M. Collins, Francisco M. Raymo
COLLOIDAL NANOPARTICLES FOR BIOMEDICAL APPLICATIONS XIII
(2018)
Article
Biochemical Research Methods
Ek Raj Thapaliya, Yang Zhang, Pravat Dhakal, Adrienne S. Brown, James N. Wilson, Kevin M. Collins, Francisco M. Raymo
BIOCONJUGATE CHEMISTRY
(2017)
Article
Multidisciplinary Sciences
Kevin M. Collins, William T. Wickner
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA
(2007)