Article
Cell Biology
Fernando Montano-Rendon, Glenn F. W. Walpole, Matthias Krause, Gerald R. V. Hammond, Sergio Grinstein, Gregory D. Fairn
Summary: PI 3-kinase is crucial for phagocytosis, with PI3,4P2, Lamellipodin, and VASP playing essential roles in actin-driven pseudopod extension and particle engulfment. Phosphoinositides are key regulators of vesicular traffic and signaling during phagocytosis, with locally generated PtdIns(3,4)P-2 influencing pseudopod progression and phagocytosis. Lamellipodin and VASP are important actin-regulatory proteins involved in phagosome formation downstream of PtdIns(3,4)P-2.
JOURNAL OF CELL BIOLOGY
(2022)
Article
Cell Biology
Lisa Dobson, William B. Barrell, Zahra Seraj, Steven Lynham, Sheng-Yuan Wu, Matthias Krause, Karen J. Liu
Summary: This study demonstrates that murine neural crest cells display actin-based lamellipodia and filopodia in vivo. Serine-threonine kinase GSK3 and cytoskeletal regulator Lpd are found to be required for lamellipodia formation while preventing focal adhesion maturation. These findings improve the understanding of cytoskeletal regulation in mammalian neural crest migration and have implications for neural crest anomalies and cancer.
Article
Cell Biology
Sara S. Parker, Kenneth Tran Ly, Adam D. Grant, Jillian Sweetland, Ashley M. M. Wang, James D. D. Parker, Mackenzie R. R. Roman, Kathylynn Saboda, Denise J. J. Roe, Megha Padi, Charles W. W. Wolgemuth, Paul Langlais, Ghassan Mouneimne
Summary: Dendritic filopodia (DF) are precursors of dendritic spines and play a critical role in synaptic formation and neural connectivity. The study reveals that the Ena/VASP protein EVL complexes with the I-BAR protein MIM/MTSS1 to promote protrusion of DF. This finding enhances our understanding of neural plasticity and has implications for the treatment of neurodevelopmental disorders.
JOURNAL OF CELL BIOLOGY
(2023)
Article
Neurosciences
Sana Al Awabdh, Florian Donneger, Marie Goutierre, Martial Seveno, Oana Vigy, Pauline Weinzettl, Marion Russeau, Imane Moutkine, Sabine Levi, Philippe Marin, Jean Christophe Poncer
Summary: KCC2, a neuron-specific chloride extruder, regulates the strength and polarity of GABAA receptor-mediated transmission and also plays a role in spinogenesis and excitatory synaptic function. The study identifies gephyrin as a novel anchoring molecule for KCC2 that stabilizes its plasma membrane expression and promotes its clustering near GABAergic synapses.
JOURNAL OF NEUROSCIENCE
(2022)
Article
Biochemistry & Molecular Biology
Seonhye Cheon, Allison M. Culver, Anna M. Bagnell, Foster D. Ritchie, Janay M. Vacharasin, Mikayla M. McCord, Carin M. Papendorp, Evelyn Chukwurah, Austin J. Smith, Mara H. Cowen, Trevor A. Moreland, Pankaj S. Ghate, Shannon W. Davis, Judy S. Liu, Sofia B. Lizarraga
Summary: ASH1L plays a role in regulating neuronal morphogenesis by counteracting the catalytic activity of PRC2. Depletion of ASH1L leads to decreased neurite outgrowth and decreased expression of the gene TrkB, which is linked to neuronal morphogenesis. The neuronal morphogenesis defect can be overcome by inhibiting PRC2 activity.
MOLECULAR PSYCHIATRY
(2022)
Article
Neurosciences
Sisu Han, Satoshi Okawa, Grey Atteridge Wilkinson, Hussein Ghazale, Lata Adnani, Rajiv Dixit, Ligia Tavares, Imrul Faisal, Matthew J. Brooks, Veronique Cortay, Dawn Zinyk, Adam Sivitilli, Saiqun Li, Faizan Malik, Yaroslav Ilnytskyy, Vladimir Espinosa Angarica, Jinghua Gao, Vorapin Chinchalongporn, Ana-Maria Oproescu, Lakshmy Vasan, Yacine Touahri, Luke Ajay David, Eko Raharjo, Jung-Woong Kim, Wei Wu, Waleed Rahmani, Jennifer Ai-wen Chan, Igor Kovalchuk, Liliana Attisano, Deborah Kurrasch, Colette Dehay, Anand Swaroop, Diogo S. Castro, Jeff Biernaskie, Antonio del Sol, Carol Schuurmans
Summary: The research reveals that Neurog2 and Ascl1 genes sustain neurogenic continuity and lissencephaly, while double+ NPCs show unique features of multipotency and play a key role in neurogenic symmetry and cortical folding.
Article
Cell Biology
Ugo Tomasello, Esther Klingler, Mathieu Niquille, Nandkishor Mule, Antonio J. Santinha, Laura de Vevey, Julien Prados, Randall J. Platt, Victor Borrell, Denis Jabaudon, Alexandre Dayer
Summary: This study reveals the regulatory role of the outer subventricular zone (oSVZ) and two microRNAs (miR-137 and miR-122) in cortical expansion, enhancing our understanding of cortical development and evolution.
Article
Chemistry, Analytical
Takaha Mizuguchi, Atsuya Momotake, Mafumi Hishida, Masato Yasui, Yasuhiko Yamamoto, Toshiharu Saiki, Mutsuo Nuriya
ANALYTICAL CHEMISTRY
(2020)
Article
Physics, Applied
Takaha Mizuguchi, Mutsuo Nuriya, Masato Yasui, Takanori Iino, Yasuyuki Ozeki, Toshiharu Saiki
Summary: Sensitive detection of alkyne-terminated ethinylestradiol (EE2) was achieved in a cetyltrimethylammonium bromide (CTAB)-coated gold nanorod (AuNR) suspension using surface-enhanced stimulated Raman scattering (SRS). The CTAB coating enabled the EE2 to exist in the plasmonic hotspot of AuNRs without binding to the surface, improving the detection limit for SRS signals from EE2 by one order of magnitude.
APPLIED PHYSICS EXPRESS
(2021)
Article
Multidisciplinary Sciences
Satoshi Yoshinaga, Minkyung Shin, Ayako Kitazawa, Kazuhiro Ishii, Masato Tanuma, Atsushi Kasai, Hitoshi Hashimoto, Ken-Ichiro Kubo, Kazunori Nakajima
Summary: The study revealed regional differences in neuronal migration profiles in different regions of the mammalian cerebral neocortex, depending on developmental stage. These differences, observed using FlashTag technology, were not well-replicated by thymidine analogs, suggesting a biological significance different from neurogenic gradient. The findings advance understanding of cortical development and demonstrate the utility of FlashTag in studying migration for future neurodevelopmental research.
Article
Pharmacology & Pharmacy
Julia Ramadhanti, Tomoko Yamada, Masato Yasui, Mutsuo Nuriya
Summary: Aquaporin-4 (AQP4) is a key water channel protein in the brain, enriched in the detergent-resistant membrane (DRM) fraction, and shows distinct molecular characteristics under oxygen-glucose deprivation conditions.
JOURNAL OF PHARMACOLOGICAL SCIENCES
(2021)
Article
Chemistry, Analytical
Mutsuo Nuriya, Yosuke Ashikari, Takanori Iino, Takuya Asai, Jingwen Shou, Keiko Karasawa, Kaho Nakamura, Yasuyuki Ozeki, Yukari Fujimoto, Masato Yasui
Summary: The development of alkyne-tagged dopamine compounds (ATDAs) provides a minimally invasive and uniquely identifiable tool to mimic dopamine in neurons and brain tissues, allowing for detailed characterization of dopamine dynamics. This approach can potentially be applied to other small-sized neurotransmitters as well, facilitating research on their dynamics in the brain.
ANALYTICAL CHEMISTRY
(2021)
Article
Multidisciplinary Sciences
Jingwen Shou, Robert Oda, Fanghao Hu, Keiko Karasawa, Mutsuo Nuriya, Masato Yasui, Bruce Shiramizu, Wei Min, Yasuyuki Ozeki
Summary: Observing multiple molecular species simultaneously with high spatiotemporal resolution is crucial for comprehensive understanding of biological systems. The super-multiplex optical imaging technique breaks the color barrier of fluorescence, achieving multiplexing number over six in living systems. Integrated stimulated Raman and fluorescence microscopy enables super-multiplex imaging with high speed and temporal resolution of seconds, providing a powerful tool to elucidate spatiotemporal organization and interactions in biological systems.
Article
Neurosciences
Debabrata Hazra, Satoshi Yoshinaga, Keitaro Yoshida, Norio Takata, Kenji F. Tanaka, Ken-ichiro Kubo, Kazunori Nakajima
Summary: This study investigates the effects of activating excitatory neurons in the superficial layers of the prefrontal cortex in a rhythmic or nonrhythmic pattern on cognitive function in mice. The results showed that rhythmic activation enhanced spatial working memory and social recognition, while nonrhythmic activation had no significant effect. This suggests that the pattern of activation plays a crucial role in cognitive enhancement.
Article
Multidisciplinary Sciences
Robert Oda, Jingwen Shou, Wenying Zhong, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya
Summary: Using SRS microscopy, we directly visualize the interactions of a deuterated analog of propofol in living cells, supporting the theory that propofol primarily interacts with the plasma membrane of neurons through non-specific binding. Additionally, we demonstrate that SRS microscopy can be used to monitor the dynamics of propofol binding in real-time.
Article
Neurosciences
Satoshi Yoshinaga, Takao Honda, Ken-ichiro Kubo, Kazunori Nakajima
Summary: This study found that the absence of Dab1 leads to the migration of superficial layer neurons into the cortical plate and mispositioning of deep layer neurons. It was also observed that Dab1-deficient superficial layer neurons accumulate below the cortical plate, while deep layer neurons show higher cell density. These phenotypes were corrected by reducing the probability of gene knockout and expressing Dab1 in deep layer neurons.
NEUROSCIENCE RESEARCH
(2022)
Article
Chemistry, Analytical
Kaho Nakamura, Keiko Karasawa, Masato Yasui, Mutsuo Nuriya
Summary: Scientists have developed a new probe for oxytocin that allows specific and highly sensitive detection of the hormone in the brain. Using this probe, they discovered high-affinity binding sites for oxytocin in the hippocampus and gained insights into the cellular basis of its volume transmission in brain tissue. The technique also shows potential for studying other centrally acting peptides.
ANALYTICAL CHEMISTRY
(2022)
Article
Neurosciences
Kota Oshima, Satoshi Yoshinaga, Ayako Kitazawa, Yuki Hirota, Kazunori Nakajima, Ken-Ichiro Kubo
Summary: This study investigated the development of the claustrum (CLA) in mice and revealed the migration profiles of CLA neurons. The findings showed that CLA neurons migrate radially outward and then change direction to migrate inward after reaching the brain surface. The study also demonstrated the essential role of Reelin signal in the distribution of CLA neurons.
JOURNAL OF NEUROSCIENCE
(2023)
Article
Biochemical Research Methods
Wenying Zhong, Robert Oda, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya
Summary: This study presents a protocol to visualize deuterated propofol in living rat neurons using stimulated Raman scattering (SRS) microscopy, which is important for clinical applications.
Article
Biochemical Research Methods
Takanori Shinotsuka, Tsuyoshi Miyazawa, Keiko Karasawa, Yasuyuki Ozeki, Masato Yasui, Mutsuo Nuriya
Summary: The spatiotemporal dynamics of substances in the brain are influenced by the water dynamics, which have been poorly understood due to the lack of suitable analytical tools. In this study, we used stimulated Raman scattering multimodal multiphoton microscopy to visualize deuterated water, fluorescence-labeled solutes, and cellular structures in live brain tissues, revealing that water diffuses more quickly than fluorescent molecules. Unlike solutes, water diffuses homogeneously in brain tissues without differences between intra- and extracellular routes. Our findings also show that water dynamics remain steady during development and ischemia, despite the severe effects on solute diffusion. Therefore, our approach provides insights into the routes and robust properties of water diffusion in brain tissues.
CELL REPORTS METHODS
(2023)
Article
Optics
Yujiro Eto, Mutsuo Nuriya
Summary: The study investigates the application of the bunching effect caused by parametric down-conversion in optical parametric generators to nonlinear imaging in biology. Experimental results show that in sufficiently intense pulse light, the bunching effect induced by ultrafast fluctuations can enhance two-photon excited fluorescence, improving biological imaging efficiency.
Meeting Abstract
Biophysics
Takaha Mizuguchi, Atsuya Momotake, Mafumi Hishida, Masato Yasui, Yasuhiko Yamamoto, Mutsuo Nuriya
BIOPHYSICAL JOURNAL
(2020)