Journal
JOURNAL OF NEUROSCIENCE
Volume 28, Issue 21, Pages 5611-5618Publisher
SOC NEUROSCIENCE
DOI: 10.1523/JNEUROSCI.5378-07.2008
Keywords
brain-derived neurotrophic factor; TrkB receptor; nicotinic acetylcholine receptor; protein kinases; interneurons; hippocampus
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Nicotinic mechanisms acting on the hippocampus influence attention, learning, and memory and constitute a significant therapeutic target for many neurodegenerative, neurological, and psychiatric disorders. Here, we report that brain-derived neurotrophic factor (BDNF) (1-100 ng/ml), a member of the neurotrophin gene family, rapidly decreases alpha 7 nicotinic acetylcholine receptor responses in interneurons of the hippocampal CA1 stratum radiatum. Such effect is dependent on the activation of the TrkB receptor and involves the actin cytoskeleton; noteworthy, it is compromised when the extracellular levels of the endogenous neuromodulator adenosine are reduced with adenosine deaminase (1 U/ml) or when adenosine A(2A) receptors are blocked with SCH 58261 (2-(2-furanyl)-7-(2-phenylethyl)-7H-pyrazolo[4,3-e][ 1,2,4] triazolo[1,5-c] pyrimidin-5-amine) ( 100 nM). The intracellular application of U73122 (1[6[[(17 beta)-3-methoxyestra-1,3,5(10)-trien-17-yl] amino]hexyl]-1H-pyrrole-2,5- dione) (5 mu M), a broad-spectrum inhibitor of phospholipase C, or GF 109203X (bisindolylmaleimide I) (2 mu M), a general inhibitor of protein kinase C isoforms, blocks BDNF-induced inhibition of alpha 7 nicotinic acetylcholine receptor function. Moreover, in conditions of simultaneous intracellular dialysis of the fast Ca2+ chelator BAPTA (10mM) and removal of extracellular Ca2+ ions, the inhibitory action of BDNF is further prevented. The present findings disclose a novel target for rapid actions of BDNF that might play important roles on synaptic transmission and plasticity in the brain.
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