4.4 Article

Phase maintenance in a rhythmic motor pattern during temperature changes in vivo

Journal

JOURNAL OF NEUROPHYSIOLOGY
Volume 111, Issue 12, Pages 2603-2613

Publisher

AMER PHYSIOLOGICAL SOC
DOI: 10.1152/jn.00906.2013

Keywords

central pattern generator; pyloric rhythm; stomatogastric ganglion; robustness

Funding

  1. National Science Foundation
  2. Integrative Graduate Education and Research Traineeship
  3. National Institutes of Health [NS-81013]
  4. Grass Fellowship
  5. Deutsche Forschungsgemeinschaft [STE 937/7-1, STE 937/9-1]
  6. Ulm University

Ask authors/readers for more resources

Central-pattern-generating neural circuits function reliably throughout an animal's life, despite constant molecular turnover and environmental perturbations. Fluctuations in temperature pose a problem to the nervous systems of poikilotherms because their body temperature follows the ambient temperature, thus affecting the temperature-dependent dynamics of various subcellular components that constitute neuronal circuits. In the crustacean stomatogastric nervous system, the pyloric circuit produces a triphasic rhythm comprising the output of the pyloric dilator, lateral pyloric, and pyloric constrictor neurons. In vitro, the phase relationships of these neurons are maintained over a fourfold change in pyloric frequency as temperature increases from 7 degrees C to 23 degrees C. To determine whether these temperature effects are also found in intact crabs, in the presence of sensory feedback and neuromodulator-rich environments, we measured the temperature dependence of the pyloric frequency and phases in vivo by implanting extracellular electrodes into Cancer borealis and Cancer pagurus and shifting tank water temperature from 11 degrees C to 26 degrees C. Pyloric frequency in the intact crab increased significantly with temperature (Q(10) = 2-2.5), while pyloric phases were generally conserved. For a subset of the C. borealis experiments, animals were subsequently dissected and the stomatogastric ganglion subjected to a similar temperature ramp in vitro. We found that the maximal frequency attained at high temperatures in vivo is lower than it is under in vitro conditions. Our results demonstrate that, over a wide temperature range, the phases of the pyloric rhythm in vivo are generally preserved, but that the frequency range is more restricted than it is in vitro.

Authors

I am an author on this paper
Click your name to claim this paper and add it to your profile.

Reviews

Primary Rating

4.4
Not enough ratings

Secondary Ratings

Novelty
-
Significance
-
Scientific rigor
-
Rate this paper

Recommended

No Data Available
No Data Available